The two-stage method of particle separation and device for its implementation

 

(57) Abstract:

The invention is intended for separating magnetic particles from a mixture containing. In the mixture injected component for separating particles form inside the vessel magnetic field to pull the separating particles on the surface of the component, collect particles from separating component at the end of the collecting component, which has the form of a tip, by application of a second magnetic field with a maximum intensity at the tip, and then carry out the transfer of particles to the second vessel and enter into contact with the separating surface. The invention solves the problem of effective transfer of particles from a large volume of small. 2 S. and 11 C.p. f-crystals, 5 Il.

The technical field

The invention relates to the separation of magnetic particles from a mixture containing, in the first vessel, with the transfer of the particles in the second vessel is substantially smaller. The invention may have various applications, especially in the field of biotechnology, biochemistry and Biomedicine.

Art

Magnetic microparticles are widely used for binding the biomaterial. One of the advantages of microparticles - this large surface area of the solid's materials and has found various applications. Magnetic particles can be moved by means of a magnet.

Commonly used methods of separating magnetic particles provide the location of the vessel of the reactor in a magnetic field so that the particles accumulate in the so-called tablet at the bottom of the vessel. After that, the liquid that is released from the particles, is decanted or sucked. However, removal of the fluid from the vessel must be very careful not to delete and particles.

In European application EP-140787 (corresponding to U.S. patent 4649116) proposed a method, according to which magnetic microparticles are separated from the liquid by using the insertion into it of the magnetic core. Particles are separated from the rod by using a stronger magnet.

In the international application WO-86/06493 proposed method, intended for use in immunological analysis, according to which the magnetic particles and the associated marked complex are separated from the fluid using a magnetic rod and then selected for measurements. At the end of the rod are fixed magnet and a removable protective housing, on the outer surface of which particles are held. After separation of the particles corpus removed together with the particles, discarded and replaced with new buildings for the next particle separation. According to the application, as the magnet can be an electromagnet so that the magnetic field can be removed when required.

In the international application WO-87/05536 offers the device and the two-stage method of separating magnetic particles from the mixture in the vessel. The device described in this application, is a component for separating magnetic particles made with the possibility of entry into the vessel. For forming the inside of the vessel magnetic field to pull particles from the mixture by separating the surface of the specified component, made in the form of a short circular cylinder, and to remove the influence of this field upon completion of the drawing process, this component is provided with a rod movable in the vertical bore of the housing, and a magnet at its lower end. According to the method, opened in this application, the component is introduced into the liquid containing the particles, when the magnet is in the lower position, causing the particles accumulated in the separating surface, located at the lower end of the rod. When the magnet means in the upper position, it is possible to branch cascio describes devices and methods for separating magnetic particles provide transfer of separated particles in another vessel, they can be used effectively in cases where it is necessary to collect particles from a rather large volume and transfer to a significantly smaller size. In particular, in the case of using any of the known device on the basis of a single component for separating particles, for example, of the type described in the application WO-87/05536, for effective separation of the particles from the mixture in a vessel large amount of surface for the Department must have dimensions commensurate with the internal diameter of the vessel. However, such surface cannot enter inside of very small vessels, for example, a cell type in the so-called HLA-Board (Board for research with human leukocyte antigen).

An additional disadvantage of the known device for the specified application is that the form of the rod with the surface for the Department in the form of a circular cylinder makes it very awkward to collect particles from the surface for the Department with any other magnetic media suitable for input into a vessel of small size.

The invention

The invention is directed to solving tasks easy and efficient collection of magnetic particles contained within a fairly large vessel, with obespecheniya new two-stage method of separating magnetic particles from a mixture by their concentration on the separating surface by pulling particles from the mixture under the action of magnetic field and subsequent collection from the surface and transfer to the second vessel, and a new device for implementing this method. The method according to the present invention includes the following operations:

put in the mixture in the first vessel component for separating particles containing separating surface;

exposure to the mixture of the magnetic field generated by the component for particle separation to remove particles from the mixture by accumulation them on the separating surface,

transfer separated from the mixture of particles held by the magnetic field, the second vessel;

the release of particles into the second vessel when removing the induced magnetic field.

The main distinctive feature of this method is that after pulling the particles, i.e., in the second stage of implementation of the method, the influence of the magnetic field generated by the component for particle separation, shoot, and collect particles from the separating surface at the tip of the collecting component, made in the form of a tip, by application of a second magnetic field with a maximum intensity at the specified edge, formed by collecting component and is concentrated at the tip, and bring the tip, the diameter of which is selected smaller OSD carried out by holding them to the specified tip under the influence of the second magnetic field.

Due to the fact that the component for separating particles not used for their transfer to the second vessel, the dimensions of the separating surface of this component can be optimally coordinated with the dimensions of the first vessel. As a result, particles can be efficiently collected even from high volume. In addition, particles can be easily transferred to another, small volume, since it uses a separate component that is optimized with respect to the transfer function.

There are also preferred embodiments of the invention in terms of both the method and the device.

Thus, a component for separating particles, preferably, provided with a magnetic core with a magnet in its lower part. In the process of separating particles from a mixture of magnetic component is inside a cavity formed by the separating surface, in the position in which particles are attracted to the surface of funds for the Department. When the particles are collected with a separating surface, this magnetic rod is moved to a position in which it no longer acts on the particles.

On the separating surface can be performed one or more grooves or similar component is C preferably has an elongated shape. Therefore, the magnetic field inside it is oriented mainly in a transverse direction relative to this component and has a large length in the direction of its length. Preferably performed on the surface of the specified component grooves for accumulation of particles were oriented in the direction of its length, i.e., perpendicular to the orientation of the magnetic field.

Collecting and removing particles from the surface of the component for separating is carried out by using a collecting component that forms the second longitudinal magnetic field intensity and gradient which reaches a maximum at the tip of the collecting component. This ensures that the concentration of collected particles on the tip of the collecting component, with which they can be transferred even in a small amount. While the application of this component facilitates the collection and concentration of particles. Collecting particles from the liquid may be conducted at any convenient time.

Forming a magnetic field with a maximum intensity at the end of the collecting component preferably is achieved by using long magnetic rod. Length magnitone 12:1.

The magnetic core preferably consists of a permanent magnet located at its lower end, and a ferromagnetic rod, which is its continuation. Due to this, the magnet and the magnetized rod together act as long magnetic rod. The rod reduces the gradient at the upper pole of the magnet, so that the upper pole does not collect particles. This way you can get the long magnetic rod at low cost. However, even in the presence of a ferromagnetic rod is preferable to use relatively long permanent magnet (the length of which is 2-10 times greater than its diameter). The length of the magnet is preferably selected in such a way to provide for this magnet the greatest possible tension constant internal magnetic field. The junction between the magnet and the rod is preferably performed so that in a short length of the rod and the magnet included in each other. The result eliminates the formation of strong gradients in the joint area, which could lead to the collection of particles in this zone.

The cross-section of the magnetic core can be, for example, round or rectangular. The round shape is the best from the point of view of manufacturing and perfectov. In principle, to simplify the drive mechanisms of the rod can be made curved.

Particles can be separated from the rod, when necessary, by removing the magnetic field generated by collecting component.

Preferably, the collecting component contained protective housing, and a rod with a magnet was made movable relative to the body.

The extremity of the protective housing (corresponding to the tip of the collecting component as a whole) is preferably provided with a downward directed arrow. This minimizes the amount of liquid remaining in the tip casing. Typical shape of the tip is a cone. When migrating particles in very small vessels of the extremity is preferably performed as a cone with a concave surface.

The shape of the protective housing on the rod collecting component depends on the specific application. As a rule, from the standpoint of manufacture and use, the most convenient round shape. To increase strength, the body can be performed tapering, which will also simplify the manufacture of the body by the method of injection molding.

The method according to the invention can be used as a simple ruchnym be used for particle sizes of 1-10 μm.

List of figures

Further, as examples, will be described some preferred embodiments of the invention. On the accompanying drawings shows:

in Fig. 1 - collecting device component according to the invention;

in Fig. 2 is a component for separating particles from a mixture, side view;

in Fig. 3 is a top view of the component according to Fig. 2;

in Fig. 4 - separation of particles from the mixture;

in Fig. 5 - collection and removal of particles from the component for the Department.

Information confirming the possibility of carrying out the invention

As shown in Fig. 1, the collecting component includes an elongated protective housing 1 in which there is a channel 2. The lower ends of the housing 1 and channel 2 is made slightly tapering. So it's easier to keep on the top end of the housing 2 has a flange 3.

Channel 2 is freely magnetic rod 4. It consists of a vertical rod magnet 5 in the lower part thereof and located above him ferromagnetic rod 6, is designed as a continuation of a rod magnet. At the end of the rod 6 has a handle 7.

The lower end of the protective housing is fitted with a tip in the form of a conical tip 8 with a concave surface. The length of this OST is approximately 10:1, and the ratio of the length of the rod to the length of the magnet is about 5:1. The stem is slightly thicker than the magnet, and the upper end of the magnet enters the lower end of the rod to a length about twice the diameter of the magnet.

In Fig. 2 and 3 presents one of the options component 9 for separating particles according to the invention, which is implemented in a fairly high first vessel 10. The vessel 10 contains magnetic particles in the mixture, which is a suspension in the liquid. Component 9 for separating particles comprises a cylindrical housing 11, the height of which is slightly greater than the height of the first vessel 10. The outer surface of the housing 11 in its part contained inside the vessel 10, forms a separating surface forming the cavity 12 with a rectangular cross-section, passing from the upper end of the body almost to its lower end. In the cavity 12 on its longitudinal (vertical) axis freely mounted magnetic rod 13, on the lower end of which has an elongated magnet 14. The field of the magnet is oriented in the transverse direction relative to the cavity (i.e., perpendicular to the image plane in Fig. 2).

The main embodiment of the cross-section of the housing 11 is highly flattened ellipse, each of the parties kotigoroshek sides of which are formed two longitudinal collecting grooves 15, appropriate to the ends of the magnet. The transverse size and the profile of the grooves aligned with the dimensions of the tip, forming the extremity 8 of the protective shell collecting component. The housing 11 has a pointed tip in the bottom part.

When the rod 13 is in the cavity 12, the magnetic particles under the influence generated by the rod 13 of the magnetic field are drawn from the mixture in the vessel 10 and accumulated in the longitudinal grooves 15 which separates the surface, forming an elongated vertical strips 16 (Fig. 4).

When you need to collect particles from strips 16 to transfer them to the second vessel, a component for separating particles removed from the vessel 10. However, due to the concentration detachable magnetic particles in the form of elongated strips in a narrow grooves 15 on the separating surface, the amount of liquid and, therefore, impurities are extracted together with the particles is very small. However, the particles are so moist that they are easy to assemble with separating surface after exposure to a magnetic field generated by the magnetic core 13 of the component 9, is removed by the discharge of this rod from separating surface of this component.

Collect particles from the grooves 15 with what hnestly component for the Department in the area of the grooves 15. Performing lower extremity of the housing 1 collecting component as the edge 8, which are provided with the maximum intensity and the maximum gradient of the magnetic field generated by collecting component using a magnetic rod 4, to optimize the mechanical contact between the separating surface and the collecting component and to create optimal conditions for particle collection.

Collected on the edge 8 of the particles is transferred to the second vessel (not illustrated) very small volume, for example of the type of cells in HLA-Board.

The extremity of the housing 1 is particularly well adapted for transport of the particles it is in very small vessels. The length of the edge in this case is slightly higher than the height of the cell. When the tip 8 is introduced into the cell, the surface of the liquid due to the action of surface tension, rises on the surface of the tip. Because when you enter the tip cell of the impact of the second magnetic field is removed (by removal of ferromagnetic rod 6 with the magnet 5, forming part of the magnetic core 4, from the lower end of the protective enclosure 1), the edge of the moving surface of the liquid washes away particles from the tip in the liquid. Separation of particles from the edge 8 may be enhanced by vzbaltyvaya, the liquid moves in the direction of the tip apex as a single film. Due to this, the liquid particles are completely separated from the edge.

In addition to these, there are other possible embodiments of the present invention. For example, deflector surface instead of two can be performed one groove 15 for concentration of magnetic particles. When using the second vessel special shaped cross-sectional profile of the tip, is made at the tip of the collecting component, may be non-circular. Therefore, in the context of the present description, the expressions "inner diameter of the second vessel and the diameter of the tip" should be interpreted as the minimum size in the transverse direction. It is also obvious that the particles allocated on the surface of the component for separating particles from a mixture that was in the vessel is relatively large volume can be migrated using collecting component not in one but in several vessels of small diameter.

1. The two-stage method of separating magnetic particles from the mixture in the first vessel, with the transfer of particles to the second vessel with an internal diameter substantially smaller internal diameter of the first vessel, by typing in the mixture is gnanam field generated component for particle separation to remove particles from the mixture by accumulation them on the separating surface; transferring separated from the mixture of particles held by the magnetic field, the second vessel; the liberation of the particles in the second vessel when removing the induced magnetic field, characterized in that after completion of the extrusion of particles removed, exposure to magnetic fields generated by the component for particle separation and collect particles from the separating surface at the tip of the collecting component, made in the form of a tip, by application of a second magnetic field with a maximum intensity at the specified edge, formed by collecting component, and bringing the tip, the diameter of which is selected smaller inner diameter of the second vessel, in contact with the separating surface, and the transfer of particles into the second vessel is carried out by keeping them on the edge under the influence of the second magnetic field.

2. The method according to p. 1, characterized in that the particles are pulled out from the mixture by separating the surface with the formation of elongated strips.

3. The method according to p. 2, characterized in that the particles pull with the formation of vertical stripes.

5. The method according to any of paragraphs.2 to 4, characterized in that the particles are pulled using a magnetic field, oriented perpendicular to the orientation of the strips.

6. The method according to any of paragraphs.2 to 5, characterized in that the particles are pulled into the groove made on the separating surface in the zone of formation of the strips.

7. The method according to p. 6, characterized in that the second magnetic field formed by the magnetic rod, movably installed at the lower end of the collecting component, and the influence of this field on the particles removed by the removal of the magnetic core from the lower end of the collecting component.

8. The method according to p. 7, characterized in that the magnetic rod collecting component is oriented along the elongated body, the ratio of rod length to thickness is chosen not less 2 : 1, preferably 3 : 1 and especially preferably 12 : 1.

9. The method according to p. 7, characterized in that the removal of the magnetic core perform longitudinal movement included in the composition of the ferromagnetic rod, to the lower end of which is attached a magnet.

10. Device for separating magnetic particles from a mixture located in the first vessel, with the transfer of the particles in the second vessel is Otdelenia particles, made with the possibility of entry into the first vessel formation within the vessel of a magnetic field to pull particles from the mixture by separating the surface of the component and removing the magnetic field upon completion of the extrusion of particles, characterized in that it further comprises a collecting component configured to collect particles from separating the surface of a component for separating particles on its tip after removal of the magnetic field generated by the component for separating particles, with the tip, the diameter of which is selected smaller inner diameter of the second vessel, made with the possibility of contact with the separating surface and the collecting component is made with the possibility of forming a second magnetic field with a maximum intensity in the zone of the tip and removing the impact of this field.

11. The device according to p. 10, characterized in that the collecting component includes a protective housing with the lower extremity in the form of points, preferably in the form of a cone with a concave surface, and the magnetic rod installed inside the protective housing can move relative to the housing in the direction of its longitudinal the outer cylindrical surface of the component for particle separation preferably in the form venutolo ellipsoid, the longitudinal axis of which is aligned with the longitudinal axis of the specified component, and an elongated magnet, designed for forming a magnetic field, is installed on the specified axis within the cylindrical cavity formed by the surface for the Department.

13. The device according to p. 12, characterized in that the separating surfaces are made one or two longitudinal grooves with a cross-sectional dimension, consistent with the size of the edge of the collecting component.

 

Same patents:

The invention relates to the separation of magnetic particles from the containing mixture and transfer them to the liquid and can mainly be used in biotechnology, biochemistry and Biomedicine

The invention relates to medicine, namely to traumatology and orthopedics, and can be used when addressing the issue of individualization mode lengthening limbs

The invention relates to medicine, namely, neurology, and can be used to differentiate multiple sclerosis and tumors of the Central nervous system, especially in cases of atypical course of multiple sclerosis and in the initial stages of pathological process
The invention relates to medicine, namely to methods for determining the content of sodium chloride in the sweat in the diagnosis of cystic fibrosis

The invention relates to medicine, namely to biophysical methods of diagnostics of various forms of neurosyphilis
The invention relates to medicine, in particular for dentistry

The invention relates to cell biology, can be used in experimental and clinical practice

The invention relates to medicine, namely cardiology, in particular to methods of selecting individual therapy during the laser

The invention relates to medicine, namely to pulmonology

The invention relates to the separation of magnetic particles from the containing mixture and transfer them to the liquid and can mainly be used in biotechnology, biochemistry and Biomedicine

The invention relates to the separation of magnetic particles from the containing mixture and can be applied mainly in biotechnology, biochemistry and Biomedicine

The invention relates to medical equipment, namely to determine the quality of liquid dosage formulations based on optical measurements

The invention relates to opto-electronic industry and can be used for a comprehensive study of the suspended particles of micron and submicron sizes (10-8- 10-4m): distribution of the particle groups with defined dimensions, chemical composition of particles, speed of change of these characteristics

The invention relates to medicine, in particular to the immunodiagnostics, and can be used to assess progenote medicinal preparations intended for parenteral administration, as well as to control contamination of products by bacterial endotoxin at all stages of pharmaceutical production
The invention relates to medicine, namely to neonatology

The invention relates to the separation of magnetic particles from the containing mixture and transfer them to the liquid and can mainly be used in biotechnology, biochemistry and Biomedicine
Up!