A method of producing human immunoglobulin against tick-borne encephalitis intravenous
(57) Abstract:The invention relates to the production of specific immunoglobulins for intravenous administration. The method consists in the fact that the allocation of specific immunoglobulin fraction conduct alcohol method at low temperatures. Fractionation of plasma donors lead to the stage of obtaining a dry Deposit of immunoglobulin, which is dissolved in 2% glucose solution, add pepsin. After proteolysis removes the enzyme aluminum hydroxide, the pH of the solution was adjusted to 6.5 and 7.5, conduct sterilizing filtration and maturation of the drug for the formation of a precipitate unstable proteins. The precipitate is removed by centrifugation at 6000 rpm and after sterilizing filtration obtain the target product. Effect: receiving intravenous immunoglobulin against tick-borne encephalitis with low anticomplementary activity. The invention relates to the production of specific immunoglobulins for intravenous administration.Raw material for producing the drug is selected according to the response inhibition of haemagglutination (rtga) plasma donors with a title encephalitis antibodies of at least 1:20. Plasma Calendarist human (HIV) and hepatitis C virusThe prototype of the invention is a method of producing human immunoglobulin against tick-borne encephalitis for intramuscular (FS 42-3155-95, the date of introduction is installed with 3.10.95 , the validity of 3.10.2000).It is known that intramuscular human immune globulin has a number of disadvantages: in the muscle breaks down to 30% of the administered drug; in the treatment of severe forms of infection, it is impossible in the short term to achieve a high concentration of antibodies in the serum of the patient; the drug has a high anticomplementary activity that prevents injected intravenously.The aim of the present invention is to obtain a human immunoglobulin against tick-borne encephalitis with low anticomplementary activity.Combined pool of immune plasma fractionary ethanol by cold; crude residue of the immunoglobulin lyophilizer; dry powder dissolved in 2% glucose solution and treated with pepsin in an acid environment within 18 hours; remove the calcium hydroxide of aluminum; bring the pH to neutral values; conduct sterilizing filtration, the maturation of the drug for the formation of a precipitate unstable proteins that remove centrifuging plasma in the amount of 360 l obtained by the method of plasmapheresis donors having natural titer of antibodies to tick-borne encephalitis virus and detected by screening. In boiler load, the title encephalitis antibody was 1:20 according to RTG.The allocation of specific immunoglobulin fraction was performed with the alcohol method at low temperatures. Received 6650 g wet sediment fraction II. The weight after drying was 2300, To receive intravenous drug 500 g of dry cake mix immunoglobulin dissolved in 2% glucose solution, bring the pH to 3.9 - 4.1 and add pepsin activity of at least 2000 IU of 25 - 50 mg per 100 g of protein. After 18 hours of proteolysis at a temperature of +37oC remove the calcium hydroxide of aluminum, bring the pH to 6.5 and 7.5, spend bleaching and sterilizing filtration and maturation of the drug for 1 - 3 months at a temperature of (6 4)oC for the formation of a precipitate colloidal-volatile components. In order to accelerate sedimentation drug thermostatic within 24 hours. The precipitate is removed by centrifugation at 6000 rpm for 40 min, hold sterilizing filter, get a 3.5 l of the finished product with a title encephalitis antibody 1:40 and above in rtga. Intravenous immunoglobulin spill the AI Hematology and blood transfusion received 8 episodes of the drug.The human immunoglobulin against tick-borne encephalitis for intravenous injection is a colorless, transparent or slightly opalescense liquid.Fraction of immunoglobulins is not less than 100% of the total protein.The protein content is about 5%.Fractional composition: immunoelectrophoresis reveals intense arc precipitation of IgG and not more than one additional arc.Molecular parameters: monomers on average 77% of the dimers is not more than 7%, fragments not more than 16%.Specific activity: the titer of antibodies to tick-borne encephalitis virus was 1:40 and above according to RTG.Compared with the prototype of the product has a low anticomplementary activity: two 50% hemolytic unit of complement (2CH50retain activity in the presence of at least 10 mg of protein.The human immunoglobulin against tick-borne encephalitis intravenous sterile, apyrogenic, non-toxic, tested in the absence of HBsAg and antibodies to HIV.The purpose of the drug treatment of patients with viral encephalitis.Currently, the human immunoglobulin against tick encephal is, , Yekaterinburg, Kirov.Thus, the resulting new drug for the treatment of patients with viral encephalitis, with significant differences from the prototype and found application in health care. A method of producing human immunoglobulin against tick-borne encephalitis for intravenous injection, comprising low-temperature ethanol fractionation of plasma donors containing antibodies to tick-borne encephalitis virus titer of at least 1 : 10 according to RTCA, characterized in that the dry residue of the immunoglobulin dissolved in 2% glucose solution, treated with pepsin in acid medium for 18 h, remove excess pepsin hydroxide of aluminum, bring the pH to 6.5 and 7.5, sterile filtration, hold the maturation of the drug at the temperature of (6 4)oC for 1 - 3 months for the formation of a precipitate unstable proteins, and to accelerate sedimentation immunoglobulin thermostatic within 24 h, remove the precipitate by centrifugation at 6000 rpm and after sterilizing filtration obtain the target product with the titer of antibodies to tick-borne encephalitis virus not less than 1 : 40.
FIELD: genetic engineering, immunology, medicine.
SUBSTANCE: invention relates to new antibodies directed against antigenic complex CD3 and can be used in therapeutic aims. Antibody IgG elicits the affinity binding with respect to antigenic complex CD3 wherein heavy chain comprises skeleton of the human variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 2, 4 and 6 and their corresponding conservatively modified variants. Light chain comprises skeleton of the rodent variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 8, 10 and 12 and their corresponding conservatively modified variants. Antibody is prepared by culturing procaryotic or eucaryotic cell co-transformed with vector comprising recombinant nucleic acid that encodes antibody light chain and vector comprising recombinant nucleic acid that encodes antibody heavy chain. Antibody is administrated in the patient suffering with malignant tumor or needing in immunosuppression in the effective dose. Invention provides preparing chimeric antibodies against CD3 that are produced by expression systems of procaryotic and eucaryotic cells with the enhanced yield.
EFFECT: improved preparing methods, valuable medicinal properties of antibody.
33 cl, 5 dwg, 1 ex
FIELD: medicine, pharmaceutical industry and technology, pharmacy.
SUBSTANCE: invention relates to a composition eliciting an antiviral effect. The composition comprises hydrophilic conglomerate of immunoglobulins consortium adsorbed with polyethylene glycol 4000-6000, recombinant interferon-α2 and a special additive taken among the following substances: glycine, glucose, maltose, sodium chloride taken in the definite ratio of components. Invention provides elevating solubility of composition eliciting an antiviral effect and enhanced release of biologically active substances to solution.
EFFECT: valuable medicinal properties of composition.
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to a composition eliciting an antibacterial effect. Composition comprises hydrophilic conglomerate of immunoglobulins consortium adsorbed with polyethylene glycol 4000-6000 and a special additive taken among the following substances: glycine, glucose, maltose, sodium chloride taken in the definite ratio of components. Invention provides sufficient desorption of biologically active substances in resuspending the composition eliciting an antibacterial effect and comprising consortium of immunoglobulins.
EFFECT: valuable medicinal properties of composition.
SUBSTANCE: the innovation deals with new immunogenic conjugates of beta-propionamide-bound polysaccharide and N-propionamide-bound oligosaccharide with protein, and the method to obtain these conjugates has been suggested, as well. Conjugates should be applied to obtain vaccines against infectious diseases and cancer that enables to broaden the number of preparations applied in treating the above-mentioned diseases.
EFFECT: higher efficiency.
1 dwg, 2 ex, 8 tbl
FIELD: microbiology and immunology, in particular immunodiagnosis.
SUBSTANCE: atypical strain of melioidose Burkholderia pseudomallei-111-6-1 with altered phenotype defected with respect to synthesis of 8 antigen and acting as immunosuppressor is used as antigen for animal immunization. Immune serum is obtained after 2 immunization cycles of animal-producer with titer in gel immunodiffusion reaction not less than 1:128.
EFFECT: immune serum with increased specific activity.
2 tbl, 2 ex
FIELD: medicine and immunology, in particular treatment and prevention immunodeficiency conditions and diseases associated with bacterial or viral aggression.
SUBSTANCE: claimed method includes administration to a patient immunoglobulin drug (e.g., pharmaceutical composition containing 6-12 % of specific heterologous secreted immunoglobulin A, isolated from milk or foremilk of immunized ungulates). Administration is performed parenterally wherein single dose is at least 10 IU/kg of patient weight for treatment or at least 5 IU/kg for prophylaxis; or perorally in dose of 0.2-0.5 g and/or topically one-two times per day for 1-5 days. Method of present invention makes it possible to decrease dose of administrating immunoglobulin due to prolonged retention of its high titers in body fluids.
EFFECT: enlarged range of application and assortment of immunoglobulin drugs.
4 cl, 5 ex
SUBSTANCE: the present innovation deals with cryoprotective ointment containing recombinant interferon-α2. The suggested cryoprotective ointment contains recombinant interferon-α2, glycerol, polyethylene glycol 300-6000, polyglucin, buffered 0.02%-Trilon B solution at pH of 5.5-7.0 and ointment foundation at a certain content of components per 1.0 g ointment. Additionally, cryoprotective ointment could contain glycine 3,7-bis(dimethylamino)phenothiazonium chloride, dry immunoglobulin preparation or dry immunoglobulin preparation for enteral application. Ointment foundation of cryoprotective ointment could contain water-free lanolin, Vaseline and Vaseline oil, at the following ratio of components: 2.5;3.5:1 - 6.5:0.5:1. The innovation provides maximal safety of recombinant interferon-α2 activity in cryoprotective ointment at multiple alteration of positive and negative environmental temperature and at keeping cryoprotective ointment under these conditions.
EFFECT: higher efficiency of application.
8 cl, 8 ex
FIELD: medicine, pharmaceutics, pharmacology.
SUBSTANCE: one should apply mammalian anti-HBP-antibodies. The ways are being suggested to identify monoclonal antibody bound, at least, with one epitope upon native HBP (heparin-binding protein) and methods to detect whether a mammal produces HBR being bound with a monoclonal antibody and, also, the kits for the above-mentioned purpose. The present innovation provides the opportunity to apply the mentioned antibodies in preventing and treating disorders associated with bradykinin releasing.
EFFECT: higher efficiency of application.
25 cl, 11 dwg, 3 ex, 1 tbl
FIELD: veterinary science.
SUBSTANCE: animals should be introduced with antihistamine serum (AHS) subcutaneously at the dosage of 4.0-5.0 ml in combination with myxoferon at the quantity of 60-75 dosages and vitamin C at the dosage of 1.0-1.5 ml/animal, once daily, thrice at interval of 5-7 d. Application of AHS in combination with myxoferon and ascorbic acid provides active stimulation of immunological reactivity, increases total body resistance I animals and causes no toxic effects and allergic reactions.
EFFECT: higher efficiency of correction.
FIELD: immunology, biotechnology, medicine.
SUBSTANCE: invention relates to antiidiotypical monoclonal antibody or fragment thereof for BSW17 antibody effecting on LgE Cε3-region bonding to high affinity LgE receptor. Amino acid sequence is as described in specification. antiidiotypical antibody is useful as pharmaceutical composition ingredient for LgE-mediated disease treatment. Invention make in possible to prevent allergic disorders and inflammations due to inhibiting interaction between LgE Cε3-region with high affinity receptor by claimed antibody.
EFFECT: new agent for allergic and inflammation disorder treatment.
7 cl, 32 dwg, 5 tbl, 10 ex