The method of obtaining health drink

 

(57) Abstract:

The invention relates to biotechnology and can be used for the prevention and treatment of cancer. Sterile liquid nutrient medium for the cultivation of mycelium of basidiomycetes filling containers with observance of the rules of asepsis, which will be added sterile agar particles up to 10% of the total volume of the nutrient medium used in the tank. This tank is made of crushed using a homogenizer Royal mycelium of basidiomycetes, obtained during the preliminary its cultivation on agar nutrient medium. Then the mycelium is cultivated on the mode of deep cultivation or on the swings at the optimum temperature for growth. Mycelial biomass agar particles in a liquid nutrient medium Packed in sterile containers for subsequent storage and delivery to the consumer. Using sterile agar particles in the composition of the nutrient medium during the cultivation of mycelium contributes to greater biomass yield of mycelium of fungi, which improves the organoleptic properties of the beverage and increases the yield of biologically active substances.

The invention relates is abuses the use of the obtained mycelium of basidiomycetes.

A method of obtaining a health drink from a solid nutrient medium after growing edible mushrooms (Iizuka Ticity, Japan (JP) B, N 62-37015, 1987). The essence of the method lies in the fact that a nutrient medium containing crushed sugar cane and/or sugar beet dried, and the thus obtained nourishing Foundation inoculant edible mushrooms grow in the normal way their mycelium, then crush nutritive base containing mycelium, add water pressurized in the tank, accelerate spontaneous fermentation mycelium and filtering the resulting suspension.

The disadvantages of this method are its great complexity, the time and the need to use complex and expensive equipment, as well as the technological complexity of the stages of this method, especially when forcing the fruiting bodies of fungi, which greatly increases the total material costs for the implementation of this method.

Also known "Method of production of hygienic drink" (European patent application N 0134257, 20.03.1985), where as raw material for this drink uses a certain type of fungi basidiomes is yemou amount of water at a certain temperature. The extract is acidified with an organic acid and maintained at a low temperature for the deposition of insoluble substances, which are then separated (by filtration). The filtrate is subjected to heat sterilization after placing it in the appropriate container.

The disadvantage of this method is that it requires special equipment for extraction at a certain low temperature and subsequent filtration of biologically active component, as well as special containers and modes of sterilization them in the obtained filtrate.

Also known as the Japan patent - Antiviral agent and method thereof (Iizuka Ticity, Japan (JP) B, application N 62-36009, 05.08.1987), where cooking tools stepwise through the following stages:

A) Cultivation of hyphae of basidiomycetes on solid or liquid nutrient medium;

B) Extraction of the active component by addition of an aqueous solvent to the resulting mycelial product cultivation;

C) Filtering the liquid extract.

The disadvantages of this method is the breeding of biologically active product a large number of water solvent and in connection with this matter which is an optional component, enter in the composition of the final product, because when it is filtered at the final stage (stage C), it is not deleted and remains in the mixture of biologically active substances. This, of course, affects the activity of the obtained substance, and the response of the host that receives or uses the final product obtained by this patent.

For the prototype we have chosen the Method of obtaining funds with radioprotective action" (SU 1824195).

The disadvantages of this method is that the extraction radioprotective means is ethyl alcohol with subsequent time-consuming and energy-intensive operation concentration under vacuum and a certain temperature (55-40 degrees Celsius), which significantly increases the cost of the final useful product. In addition, the final product always found the presence of residual ethanol in 1-2%, and it is not always desirable for the consumer.

The proposed method of obtaining a health drink from the mycelium of basidiomycetes in stages as follows:

stage 1: Preparation of sterile liquid culture medium for subsequent cultivar the vision of the rules of asepsis, where additional paid sterile food grains agar to 10% of the total volume of the nutrient medium used in the tank.

stage 3: Making crushed using a homogenizer uterine mycelium of basidiomycetes, obtained during the preliminary its cultivation on agar nutrient medium.

stage 4: Performing cultivation of mycelium on the mode of deep cultivation or rocking at a temperature of 25-27 degrees Celsius growth.

stage 5: Packing wavesi mycelial biomass agar grains in a liquid nutrient medium, where the development of the mycelium was separated biologically active substance in sterile containers for subsequent storage and delivery to the consumer.

Thus, the main distinctive advantages of the proposed Wellness drink are the following:

- full use of the nutrient medium without dilution of biologically active substances obtained during cultivation of mycelium;

- no extrarenal type of ethyl alcohol in the final product;

- use sterile agar grain in the composition of the nutrient medium during the cultivation of mycelium leads to the TV in a confined volume of liquid nutrient medium. This results in greater biomass yield of mycelium of fungi, which improves the organoleptic properties of the beverage and increases the yield of biologically active substances;

- use the drink sterile grain food agar prepared in aseptic conditions, increases the adsorption abilities of the drink, because the individual particles of agar, acting in this case as carriers of mycelial biomass, retain their adsorption capacity;

- save after packaging of the finished product agar particles with a viable mycelium of basidiomycetes significantly increases the shelf life of this product;

- obtaining a health drink in a clean environment closed cultivation allows you to prepare biologically active product with a significantly greater biological activity and adsorption capacity in relation to the salts of heavy metals and radionuclides, as well as with a large saving of material resources.

We offer a health drink used for prevention and treatment of cancer, the removal of radionuclides from the human body, is used as immunine mycelium of basidiomycetes, homogenization, making the crushed mycelium in liquid nutrient medium, deep cultivation at 25 - 27o, Characterized in that the culture medium was additionally contribute sterile agar particles up to 10% of the total volume of the nutrient medium used for submerged cultivation of mycelium of basidiomycetes, and Packed a suspension of mycelial biomass in sterile conditions.

 

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