The method of therapy of pollinosis

 

(57) Abstract:

The invention relates to medicine, Allergology. Spend endonasal electrophoresis of the supernatant of the culture of autologous mononuclear cells stimulated phytohemagglutinin. The method reduces the time of treatment.

The invention relates to medicine, in particular to clinical immunology and Allergology. It can be used in the clinic to treat patients with pollinosis.

It is known that one of the well-known methods of treatment of allergic rhinitis is specific immunotherapy (SIT). According to the literature there are many methods of SIEVES, including specific electrophoretic immunotherapy (E. I. A. Salimov M. Nazarov. Heredity and comparative effectiveness of various methods specific hyposensitization with allergic rhinitis in children - Pediatrics, 1989, N 6).

There is a method of treatment of hay fever by endonasal electrophoresis intala, in the course of 10 treatments (Bezpalko N. N. and other "Endonasal electrophoresis intala for the prevention and treatment of hay fever in children in the conditions of sanatorium", published in proceedings of "Current issues of sanatorium-resort treatment of children with tubercular diseases." what is what clinical effect in the appointment of membrane stabilizers, including intala, is only possible with continued use, in the conditions of the electrophoretic method of introduction can be shown not all patients.

The essence of the proposed method is that the patient is in remission of the disease introduced by electrophoresis endonasal supernatant of the culture of autologous mononuclear cells (MNCs), stimulated phytohemagglutinin (PHA), in the amount of 1-2 ml per procedure, with a protein concentration of 150-200 μg/ml for 10 days.

Introduction to shock the body supernatant autologous MNCs ensures the supply of natural cytokines (COC), such as interleukin-1, interleukin-2, interleukin-6, interferon and tumor necrosis factor, triggering local cytokine cascade. At the same time, - interferon, interleukin-2 control allergic response through the regulation of production by b-lymphocytes immunoglobulin E.

The ability electrophoretic introduction of the PCC with the positive pole is due to the fact that they are acidic polypeptides of low molecular weight (<80 KD).

Endonasal the introduction of the PCC occurs due to not only the local, but the overall response of the whole organism. As you know the positive effect of DC is not only in a variety of physico-chemical metabolic and cellular reactions aimed at mobilizing protection systems, but also to create a favorable background for the specific action of substances introduced with it.

When selecting the dosage of the input complex, we have taken into account the fact that in an electrophoretic method of introduction into the body receives a small percentage of drug substances, in contrast to the application of the application of the PCC on the wound surface. Dosage for protein, given the above, increased to 150-200 mcg/ml in comparison with the use of PCC in other types of pathology.

The amount of COC in each procedure can vary from 1.0 to 2.0 ml depending on the physiological characteristics of the shock body and chosen by us because it is this amount of fluid is necessary and sufficient for one procedure endonasal electrophoresis.

The method is as follows. The patient in the period of remission collect 20 ml of venous blood from the cubital vein into sterile siliconized tube, aderente density ficoll-urografin. The selected cells are washed three times with a solution of Versene and prepare a suspension in their concentration 5106ml in medium 199. To the resulting suspension type 80 μg/ml gentamicin and PHA rate of 20 μg/ml, then incubated for 3 hours in a thermostat at a temperature of 37oC. Remove the stimulator, three times washing with a solution of Versene continue to cultivate 20 hours in serum-free medium at a temperature of 37oC. Separating autologous supernatant by centrifugation of the suspension of mononuclear cells for 10 minutes at 1500 rpm, the Supernatant filled into sterile vials of 2 ml and stored at a temperature of - 20oC. the remaining cell draught add medium 199 and gentamicin to the original volume, incubated in a thermostat at a temperature of 37oC for 24 hours. Autologous supernatant is separated in a similar way. These procedures are performed under sterile conditions in compliance with the rules of asepsis and antisepsis. The resulting supernatant filtered through sterile filters synpore or millipore 0,22. The biological activity of the supernatant evaluated in the test phagocytic activity of neutrophils. The total protein content in the supernatant is determined according to Lowry spectrophotometric method.

Sick electrophoresis with the positive pole of the apparatus Thread-1 for 10 days. The current density in an electrophoretic introduction of supernatant from 0.2 to 2 mA/cm2the duration of the procedure from 10 to 25 minutes depending on the individual sensitivity of the organism to DC.

Example. Patient L..., 36 years old, diagnosis at admission: hay fever; the sensitization to pollen and grass cultivated cereals; rhinoconjunctivitis syndrome; tracheobronchitis moderate severity, the interseasonal period of remission.

Blood test on admission: e-4,551012/l; Hb 149 g/l; C. P.-0,99; L-4,41109/l; e-3% n 3% C-69%; LF-18%; M-6%; ESR-3 mm/hour. The immunological: E-ROCK-80%; TGF-ROCK-60%; TFC-ROCK-8%; IRI-7,5; PHI-60%; FC-7,0; IgA-1.5 g/l; IgM-0.55 g/l; IgG-8.5 g/l; IgE-273 IU/ml; CEC-0.9 g/l of Skin testing with pollen allergens: cocksfoot++++; fescue++++; bluegrass +++; ryegrass +++; Timothy ++; rye; Alopecurus -. Provocative nasal test is positive when the concentration of allergen cocksfoot 10-5.

The patient before Palencia plants were introduced into the nasal cavity by total nasal move the PCC method endonasal electrophoresis with the positive pole of the apparatus Thread-1. The volume of supernatant for each procedure was 2 ml, the amount of protein in the supernatant 150 μg/ml, the duration of the procedure up to 20 minutes, to which were short-term, independently intractable subjective feelings in the form of nasal congestion, rinorea, sneezing and itching eyes.

After 10 treatments (after treatment) blood test: e-4,311012/l; Hb-142 g/l; C. P-0,99; L-6,44109/l; e-2%; n 5%; C-57%; LF-24%; M-12%; ESR-2 mm/hour. The immunological: E-ROCK-60%; TGF-ROCK-64%; TFC ROCK-16%; IRI-4,0; PHI - 62%; FC-6,0; IgA-1.4 g/l; IgM-1.0 g/l; IgG-7,05 g/l; IgE-226 IU/ml; CEC-0.6 g/L. the sensitivity of the shock body Jerzy team with provocative natalina test decreased by 1 point (10-4) in comparison with (10-5). The degree of sensitization to pollen allergens Jerzy team, Timothy, fescue, bluegrass, ryegrass in the control of skin testing remains the same.

In a follow-up period in the period of Palencia plants watched the severity of clinical symptoms of volume of drug therapy and duration of exacerbation.

In a follow-up period after Palencia plants conducted an assessment of the effectiveness of treatment on a scoring system (A. D. ADO), it was 3 points (good).

The study included 16 patients in remission of the disease. All patients were divided into two groups: main and control. When scoring indicators of clinical symptoms in obese from 15 to 36 years, the average age 29,8 years, 5 of them are men, women - 2; control consisted of 9 people aged from 19 to 35 years, with an average age of 27.7 years, men and 4 women 5.

Patients of the main group has introduced the PCC method endonasal electrophoresis N 10, patients in the control group spent sit in an expedited manner by subcutaneous injection of allergens.

In a follow-up period in the period of Palencia plants watched the severity of clinical symptoms of volume of drug therapy and duration of exacerbation. The effectiveness of the treatment in each group was evaluated in a follow-up on a scoring system A. D. ADO. In the endonasal electrophoresis introduction the PCC has been a clear tendency to decrease the severity of clinical symptoms during Palencia plants.

Using the proposed method of treatment of patients with pollinosis has the following advantages:

1. Procedure of introduction of the PCC atraumatic;

2. The reduction of treatment time;

3. No risk of reactions to allergens.

The method of treatment of hay fever by endonasal electrophoresis medicinal substances, the course of 10 treatments, characterized in that zolirovany phytohemagglutinin, in quantities of 1 to 2 ml with a protein concentration of 150 - 200 µg/ml

 

Same patents:
The invention relates to medicine, gastroenterology

The invention relates to medicine, more specifically to a method of normalizing the physiological state by drug therapy and physiotherapy, and can be used for the prevention and treatment of a wide range of diseases

The invention relates to medicine, obstetrics

The invention relates to medicine, physical therapy

The invention relates to medicine, namely to the section of gastroenterology and for the treatment of patients with peptic ulcer physical methods

The invention relates to medicine, physiotherapy, gynecology
The invention relates to medicine, physiotherapy, andrology

The invention relates to medicine, physiotherapy, neurology

The invention relates to a medicine for the treatment of encephalopathy (e.g., the liquidators of consequences of the accident at the Chernobyl nuclear power plant)
The invention relates to medicine, in particular to the treatment of diseases characterized by autoimmune aggression

The invention relates to medicine, more specifically to the Neuroimmunology, namely to test for antigens associated with multiple sclerosis

The invention relates to medicine, in particular to Oncology

The invention relates to pharmaceutical industry and the receipt of liofilizirovannogo preparation of alpha-fetoprotein (AFP), belonging to the group of immunomodulators and used as a treatment for cancer

The invention relates to the field of medicine and are expected to be used in the treatment of malignant tumors
The invention relates to the food industry

The invention relates to medicine, specifically to pharmacology, and relates to the use of previously known antimicrobial colloidal preparations of silver of the protargol and colloid for a new purpose as antihypoxants

Eye film // 2076672
The invention relates to medicine, in particular to ophthalmology, and can be used in the treatment of traumatic injuries, trophic disorders and chemical burns of the cornea, conjunctiva
The invention relates to medicine, namely to a method for producing an anti-shock solution plazmozomenauchi
Up!