A method of treating patients with demyelination diseases

 

(57) Abstract:

The invention relates to medicine and can be used in the treatment of patients with demyelinizing disease of the nervous system such as multiple sclerosis. Impose a suspension of live fetal human cells corresponding to the affected sections of the brain and spinal cord of the patient, in lymph node volume 05-10 ml, using the number of cells is of the order of 15-35 million. Manipulation spend 3-4 times with an interval of 5-7 days between manipulations. The method reduces the risk of complications and reduce the number of exacerbations of the disease and prolong the period of remission. table 1.

The invention relates to medicine and can be used in the treatment of, for example, patients with demyelination diseases.

The essence of the method is that the patient with demyelinizing disease of the nervous system such as multiple sclerosis. impose a suspension of live fetal human cells corresponding to the affected sections of the brain and spinal cord of the patient's lymph nodes in the amount of 0.5-1.0 ml, the number of cells is of the order of 15-35 million, this manipulation spend 3-4 times with an interval of 5-7 days between manipulative (see Table).

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However, this method is difficult to perform and requires specific skills to carry out [1,2].

As a prototype method selected intravenous fetal human cells [2].

The disadvantage of the prototype is that this procedure has a high risk for side effects and requires special skills.

Medico-technical objective of the proposed invention is the decrease in the rate of disease progression and achieve long-term remission.

This problem is solved by the fact that the patient with demyelinizing disease of the nervous system such as multiple sclerosis, impose a suspension of live fetal human cells corresponding to the affected sections of the brain and spinal cord of the patient's lymph nodes in the amount of 0.5-1.0 ml, the number of cells is of the order of 15-35 million, this manipulation spend 3-4 times with an interval of 5-7 days between manipulations.

This technique is performed in the following way [5]. From purified fetal cells of the second trimester of pregnancy, by selected recipe [5] to prepare a suspension. The method is performed in the following sequence. For social reasons produce controlled abortion. Of the embryo is a, relevant affected sections of the brain and spinal cord of the patient, also in this suspension add elements of the endocrine and immune systems of the embryo, for example, liver cells, spleen, thymus gland, to correct abnormalities of the specified systems of the patient.

The obtained suspension is injected to the patient's lymph nodes in the amount of 15-35 million cells in a volume of 0.5-1.0 ml of the Introduction of less than 15 million fetal cells is not effective, due to the weak effect, and the use of more than 35 million cells is accompanied by the occurrence of negative side effects. Preparation of suspensions of fetal cells in a volume less than 0.5 ml leads to increased aggregation, which affects the functioning of cells, and the use of suspensions of more than 1.0 ml of this method of introduction may cause damage to the lymph node.

The specified treatment repeat 3-4 times with an interval of 5-7 days. Repeating this procedure, the introduction of fetal human cells in lymph node less than 3 times does not lead to a pronounced positive results. It was established experimentally that 3-4-fold introduction of fetal cells in the lymph node provides long-lasting positive effect without further the Nations. Delay refresher on time, more than 7 days reduces the effectiveness of the treatment.

Example 1. Sick So 49 years with a diagnosis of multiple sclerosis, spinal form. Complaints about General weakness, shortness of movement of the limbs, dizziness, fatigue. In neurological status: FMN without pathology, tetraparesis more pronounced in the lower limbs, tendon reflexes high, quickened D=S, muscle tone increased by spastic type, coordinatorsee samples with intention, in the Romberg staggers. For the patient was prepared suspension of fetal cells corresponding to the affected sections of the brain and spinal cord of the patient 20 million in volume 0.7 ml of This suspension was injected 3 times with an interval of 6 days. After the introduction of fetal cells in the lymph node was observed stabilization of the immune status, the normalization of the level of interferon; improved General condition, slightly decreased muscle tone, better run coordinatorsee samples became more stable in the Romberg position. Extending the period of remission, reduced the number of exacerbations during the year approximately in 2 times.

Example 2. Patient B., 32 years old with a diagnosis of multiple sclerosis, spinal form. Complaints of weakness, slagstone FMN without features, left-side hemiparesis to 3-3,5 points, tendon reflexes increased left, right - reduced muscle tone is increased, more pronounced on the left, coordinatorsee samples uncertainly, with intention, worse on the left, in the Romberg unstable. Patient was prepared suspension of fetal cells corresponding to the affected sections of the brain and spinal cord of the patient 30 million in volume 1.0 ml of This suspension was injected 4 times with an interval of 7 days. On the background of treatment with the introduction of fetal cells in the lymph nodes four times the patient's condition significantly improved muscle tone has improved, coordinatorsee sample is satisfactory, in the Romberg position became more stable. Stabilized immune status, normalized level of interferon. Lengthened the time of remission, reduced the number of exacerbations during the year, approximately 2.5 times.

The efficiency of the proposed method is shown in the table, where it is additionally a comparison with the prototype.

Comparison with the prototype shows:

- reduced the risk of complications is only 2 patients out of 9, compared with the prototype 4 of 17.

unlike the prototype, this method allows to reduce the number of exacerbations of the disease and ADLINK., Dry, So Endolyumbalno method neurotransplantation fetal cells of the human brain in neurology. - The Medical Market.- N3, 1995 - S. 84-87.

2. Mironov N. In., Shmyrev Century. And., Bugaev B. C., Anikin, A. Y. Application of neurotransplantation fetal cells of the human brain in the treatment of certain neurological diseases. - Transplantation of fetal tissue and human cell - Sat. Art.) 1996 - S. 50-52.

3. Levin Y. M. foundations of medical lymphology. - Moscow, "Medicine". - 1986

4. Polezhaev L. C., Alexandrov, M. C. Transplantation of brain tissue in health and disease. -M., 1986.

5. Mironov N. In., Shmyrev Century. And., Bugaev C. S., Mironov, I. N., Dry, So a Method of treatment of neurological patients by the intravenous injection of fetal human cells - application N 96112682 from 8 iuoe 1996

A method of treating patients with demyelination diseases through the introduction of fetal cells, characterized in that the patient with demyelinizing disease of the nervous system such as multiple sclerosis, impose a suspension of live fetal human cells corresponding to the affected sections of the brain and spinal cord of the patient's lymph nodes in the order of 0.5 - 1.0 ml, the number of cells of about 15 to 35 million, this man is

 

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