The method of evaluation of the spinal cord
(57) Abstract:The invention relates to medicine, can be used in clinical laboratory diagnostics, in particular for assessment of functional state of the spinal cord in patients with spinal injury. The patient under local anesthesia by a lumbar puncture spinal cord, get the liquor. On a special object glass put two drops of the liquor. In one drop add drop of 0.9-aqueous NaCl solution. Every drop cover cover glass. After drying at room temperature the drugs studied in polarized light using a light meter, determine the transmittance in the optical units. Calculate the index of the functional condition (ANDf) as the difference between the transmittance of native CSF and CSF in saline environment and the importance of ANDf0,035 determine quickly progressing processes of disorders of the spinal cord. In this case the patient carry out urgent surgical treatment. When the value off0,035 evaluate processes of disorders of the spinal cord as a stable and decide about the choice of tactics of treatment. Technical result: simply, quickly and accurately activley patients and prevent disability. The method relates to medicine, can be used in clinical laboratory diagnostics, in particular for assessment of functional state of the spinal cord in patients with spinal injury.Known methods of assessment of the functioning of the spinal cord to study the cellular and chemical composition of the liquor, which allow to evaluate the properties with diagnostic value lymphocytosis, protein, glucose, chloride, etc. (Laboratory research methods in the clinic. Handbook edited by Prof. centuries Menshikov. M.: Medicine, 1987, S. 101-105).These methods have some diagnostic information content only if the integrated use. Studies of the cellular and chemical composition require a large amount of liquor, and a large number of clinical and biochemical techniques that use expensive reagents or test systems.Closest to the present invention is a method crystallographic studies of lacovara (C. I. Neretin, C. A. Kiryakov Crystallographic method studies of the cerebrospinal fluid in diseases of the Central nervous system, J. Soviet medicine, No. 7, 1977, S. 96-102).The method According to crystallographic picture of the liquor define diseases of the Central nervous system of the patient - the presence of tumor or inflammatory processes.However, the functional state of the spinal cord is not evaluated. In addition, the disadvantages of this method are: the use for research of a large number of CSF (2.0 ml); the duration of microscopic analysis (20 min); microscopic (subjective) view of the results of the study (on crystallographic pattern); low specificity of the method.The task is to assess the function of the spinal cord of patients with spinal injury, a simplified method of evaluating and improving its accuracy, is solved as follows.According to the invention, study of structural and optical properties of native and liquor in saline medium polarized light, determine the transmission coefficient, calculate the index of the functional condition (ANDf) as the difference between the transmittance of native CSF and CSF in saline medium, and when the value off0,035 determine rapidly progressive disorders of the functional state of the spinal cord, and a value off< a 0.035 evaluate processes of disorders of the spinal cord of the patient as stable.Structure-optical property is simple in execution, does not require expensive reagents, allows the quantification of structural-optical properties in a minimal volume of liquor only 0.02 ml (in the prototype 2.0 ml).The accuracy and information content of the method is proved by the authors experimentally on 18 patients in the Ural research Institute of traumatology and orthopedics.Patients with rapidly progressing processes of violations of the functional state of the spinal cord (ANDfa 0.035) in the early and acute periods of cerebrospinal trauma (PSMT), was carried out by urgent surgical intervention. The function of the spinal cord was fully restored, allowing the patient to return to the earlier work.Patients who have functional state of the spinal cord, the proposed method was defined as stable (ANDf< a 0.035) - the treatment of choice was conservative or operative, aimed at the destruction of pain, to increase the motor activity of the patient, the stimulation of the lost functions of the spinal cord, orthopedic correction and stabilization of damaged spine with elements closed decompression of the spinal cord and meningoradiculitis.
SUBSTANCE: method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.
EFFECT: accelerated examination process; reduced risk of traumatic injuries of fetus or newborn.
FIELD: veterinary medicine.
SUBSTANCE: method involves carrying out blood erythrocyte micro electrophoresis in alternating electric field with sign change frequency equal to 0.3-1 Hz. Electric field having current intensity equal to 4.5-5.0 mA is applied. Treatment is applied at 36-38°C. Oscillation amplitude being equal to 3 mm and less, endotoxicosis is detected.
EFFECT: high accuracy and simplicity of the method.
SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.
EFFECT: high reliability of diagnosis.
FIELD: analytical methods in medicine.
SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.
EFFECT: increased drug selection efficiency.
FIELD: medicine, hepatology.
SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, otorhinolaryngology.
SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.
EFFECT: higher efficiency.
5 dwg, 3 ex, 2 tbl
FIELD: medical engineering.
SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.
EFFECT: high reliability of analysis results.
20 cl, 9 dwg
SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.
EFFECT: higher efficiency and accuracy of diagnostics.
1 cl, 9 dwg, 5 ex, 1 tbl
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.
EFFECT: higher accuracy of differential diagnostics.
3 dwg, 2 ex, 1 tbl
FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.
SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.
EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.
3 cl, 4 dwg