A method of treatment of diseases of the central nervous system

 

(57) Abstract:

The invention relates to medicine and can be used in the treatment of diseases of the Central nervous system. Patients in the CSF space of the brain give 1 ml suspension of embryonic brain tissue with nucleic acid-protein extracts of this fabric in an amount of 0.01-0.05 mg of dry residue of the mixture per 1 kg of patient's weight with the subsequent introduction of a similar dose of the solution of the extract administered 4 times a day 2 drops in each nostril for 7 days. The method provides interaction implantable material with brain tissues of the patient and has the advantage of efficiency and limited in comparison with known methods.

The invention relates to medicine, namely to methods for treating diseases of the Central nervous system.

Known methods of treatment of diseases of the Central nervous system with the use of embryonic and fetal human tissues. Used for this injection route of administration of these tissues subcutaneously or intramuscularly [1]. However, this method is effective only in early childhood [1]. There is also known a method of transplantation of embryonic and fetal tissues by ogicheskom intervention [2, 3]. The disadvantage of this method is its low efficiency at high morbidity and limitations associated with the impossibility of its use in somatically debilitated patients [3].

Closest to the proposed method - transplantation of fetal neural cells in the subarachnoid space of the spinal cord via lumbar puncture [4] . The limited way in using only the cellular components of implanted fetal tissue.

An object of the invention is a method of treatment of diseases of the Central nervous system with a higher efficiency and less limitations.

The problem is solved by the method lies in the fact that the CSF space of the brain produce the introduction of a mixture of 1 ml of suspended embryonic tissues of the human brain together with protein-nucleic acid extracts of this fabric in an amount of 0.01 - 0.05 mg extract per 1 kg of patient's weight with the subsequent introduction of a similar dose of the solution of the extract route to 2 drops in each nostril 4 times a day for 7 days.

The method is as follows. 1) Pre make the extraction of proteins and , is sterilized and freeze-dried. Just before the medical procedure prepare drugs for injection, sterile conditions, remove 1 ml of brain bubble their embryo or human fetus, suspensorium it in 2 ml of physiological solution pipettes to a homogeneous state and the resulting solution was added to the extract at the rate of 0.01 - 0.05 mg extract per 1 kg of patient's weight. As primary source material used donors whose research has demonstrated the absence of contamination by viruses, AIDS, herpes, hepatitis A and B, and with a negative Wasserman. 2) Using standard methods, penetrate into the CSF space of the Central nervous system transcranial, suboccipital lymphadenopathy or abandonable accesses. 3) Extract the cerebrospinal fluid of a patient in excess of the amount of an administered drug in 2 times. 4) the Drug is slowly injected from a syringe needle through the needle into the CSF space of the brain. 5) Lyophilized protein-nucleic acid extract was dissolved in 8 ml of sterile water for injection and buried nose 4 times a day 2 drops in each nostril for 7 days.

Example 1. Patient K., 59 years old. Admitted to the neurology hospital with globigii self-service the stiffness of the legs when walking, dragging pain in the muscles. Anamnestically the patient is suffering from Lou Gehrig's disease, the diagnosis is verified in the leading neurological hospitals of the Russian Federation. Effect from the classical methods of treatment were not observed, the disease has steadily progressed. When inspecting a state of moderate severity, dominated pathology of skeletal muscle, which manifested itself in the spastic-atrophic paralysis of the extensors of both feet and hands. Marked atrophy of the shoulder muscles, muscles of the exaltation of the thumb and little finger of the right and left hand, atrophy of the muscles of the forearm. Notes fascicularia deltoids, the muscles of the forearms and shoulder girdle. Legs are expressed tendon hyperreflexia no difference parties. Disorders of sensitivity is not revealed. Diagnosis is confirmed by the results of biografii and magnetic resonance imaging. On the background of integrated traditional therapies conducted suboccipital lymphadenopathy implantation in a CSF space of the brain of the patient a mixture of suspended fabric of the human brain obtained at the stage of prenatal development, together with protein-nucleic acid extracts of this tissue total volume of 3 ml of 0.01 mg extract per 1 kg of the patient, the latter is the philosophy of muscles decreased, disappeared zapadenia interdigital intervals, the patient increased muscle mass of 2.5 kg, was independently eat, comb, improved letter, dramatically reduced the spasticity in my legs has disappeared fascicularia trunk muscles.

Example 2. Patient M. , 37 years. Hospitalized with complaints of pain in the lower back and lower extremities, as well as in the neck and shoulder blades. Pain wore paroxysmal unpleasantly painful character. Complaints of weakness in his right leg, which the patient could not walk more than 10 meters, a sharp decrease in surface sensitivity. On examination: the patient titration, on the skin of the hands and feet increased scars painless burns, severe hypotrophy of the right gluteal muscles and the right leg. Legs prepares on pyramid gipu. Peripheral paresis of the right hand, accompanied by a decrease in tendon reflexes. Reduced pain and temperature sensitivity on the right arm and the legs, when the safety of the musculoskeletal and tactile. Based on the data of computer tomography and magnetic resonance imaging were diagnosed to those of syringomyelia, slowly progressive course. Conducted endolyumbalno introduction to the CSF space of the brain of the patient mix suspensionsystems this tissue total volume of 3 ml of 0.01 mg extract per 1 kg the weight of the patient, followed by instillation of a solution of the extract administered for 7 days. A positive result was obtained in six months. The patient began to walk up to 2 to 3 km per day, restored muscle mass right buttocks, decreased discomfort in the legs, became active, energetic, took the first group of disability.

Example 3. Patient A. 18. Complaints adequately state does not show. According to his mother, and medical documents 13 months ago, I was in a car accident on a motorcycle. He received a severe contusion, subarachnoid hemorrhage, acute subdural gidromy left and right, hemopneumothorax right, traumatic shock 3 degrees. Conducted resection trepanation in the right and left temporal areas, removed subdural gidromy. In the treatment process developed purulent meningoencephalitis. Diagnosed with post-traumatic akineticalkie mutism imposed by tracheostomy. After completing the course of treatment prescribed for home surveillance. Objectively admission: patient contact is not available, obrezannuyu it does not understand the command does not execute. Contracture of the muscles of the limbs, movements in the limbs not expressed tetraparesis limbs more unique is good symptoms Babinski, Oppenheim, Gordon, Rossolimo on both sides. Positive phenomena of oral automatism. Conducted introduction to the CSF space of the spinal cord of the patient a mixture of suspended fabric of the human brain obtained at the stage of prenatal development, together with protein-nucleic acid extracts of this tissue total volume of 3 ml in the amount of 0,05 mg extract per 1 kg of patient's weight. The patient regained consciousness, understands addressed speech, performs basic instructions: opens and closes his eyes, learns relatives, holding his head, turns it to the side, his tongue out for the team, trying to pronounce the sounds began to raise his right hand, waving "goodbye", was the beam to swallow, there were movements in the fingers and toes.

The proposed method of treatment used in 7 patients. The insertion procedure is carried out in a neurological hospital, during the first days after the procedure shown in bed and symptomatic analgesic therapy is indicated. All patients not identified individual intolerances and allergic reactions. Contraindication for the procedure is the presence in a patient of acute inflammatory process.

Sources of information

1. the Aoun in children.//Bully experts. Biol. the honey. - 1994. - T. 117, No. 4. S. 373 - 374.

2. Vinogradov, I. N. The neurotransplantation in the treatment of diseases of Parkinson//Ukr. questions neurosurgery. - 1993. - N 1. - S. 35 - 38.

3. Polezhaev L. C., M. Alexandrov A. Transplantation of the brain in health and disease. - M.: Nauka, 1986. - 152 C.

4. Mironov N. C. and et al. Endolyumbalno method neurotransplantation fetal cells of the human brain in neurology. The Medical Market, M., 1995. 3. 19. page 84 - 87.

5. Zbarskaya I. B. , Chemistry and biochemistry of nucleic acids. M.: Medicine, 1968. 430.

6. Orekhovich C. N., Analytical methods of protein chemistry. M., 1963, 644.

A method of treatment of diseases of the Central nervous system, including the introduction of embryonic brain tissue of a person in a CSF space of the brain, characterized in that the injected 1 ml of suspended embryonic brain tissue with nucleic acid-protein extracts of this fabric in an amount of 0.01 - 0.05 mg per 1 kg of patient's weight with the subsequent introduction of a similar dose of the solution and the extract is administered 4 times a day 2 drops in each nostril for 7 days.

 

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FIELD: medicine, otorhinolaryngological surgery.

SUBSTANCE: one should apply thin layer of "Solcoseryl" gel onto osseous facial walls of frontal and maxillary sinuses at the border with trepanation opening after removing pathological content out of them and before applying a transplant out of flat bone of human fetal cranial arch that exceeds the diameter of trepanation opening by 3-4 mm. Then, one should additionally fix the transplant by affecting with distal edge part of a light guide of semi-conductor laser "ATKUS-15" with contact-type technique at output power of laser radiation being 8 W at constant mode. The method enables to increase fixation density of allobrefobone to osseous walls of sinus along its whole diameter.

EFFECT: higher efficiency of fixation.

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