The method of obtaining funds with radioprotective activity
(57) Abstract:The invention relates to the field of pharmacy and concerns receiving means having a radioprotective effect. Mixed lichens, as well as excipients lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup in the following ratio, wt.: lichen 50, lactose 25, starch 25. The method allows to extend the range of natural plant materials to obtain protective remedies. table 2. The invention relates to the field of pharmacy and relates to a method of receiving means having a radioprotective effect.Due to the increased threat of increasing radiation pollution, as well as features of chronic radiation sickness and the ability of the damaging effects of radiation to cumulative effects (the summation), the ongoing risk of exposure of radionuclides in the body and their accumulation in the tissues, especially important is the use of radioprotective drugs that do not have side effects and harmless with long-term use. This problem is particularly acute for residents of the areas affected by the accident at Cannabalizing action occupy a special place drugs of natural origin as a means of prevention and treatment of radiation injuries, having several advantages over synthetic analogues.First of all natural radioprotectors promising under the action of small doses of radiation and chronic exposure, low toxicity and capable of long-term save activity when administered orally.Synthetic drugs, such as selenium or sulfur, used most often with simultaneous irradiation with a powerful dose and is not used for chronic radiation sickness, because these substances can accumulate, leading to their toxic effect on the body (1).Among the natural radioprotective tools there are tools like animal poisons, extragenic, immunomodulators, isolated from different tissues) and plant origin (photoadaptive, polysaccharides, alkaloids), are able to exert a specific action and to improve the overall nonspecific resistance of the organism (2, 7, 8). However, some of them, for example, an extract of St. John's wort (Hypericum perforatum L.), according to the latest data (9), which has a radioprotective effect, has limitations apply burns, accompanying radiation damage, because it is characterized by slowing of the healing process.Main mA the teachings of the finished product.Upon receipt of the extracts of the starting substance (e.g., plant material) is subjected to prolonged heating, which can lead to the loss of biologically active substances. For example, when the extraction of plant raw materials containing such compounds as iridoid, when heated to temperatures above 40oC lost a significant portion of biologically active substances.The objective of the proposed technical solution is the expansion of the range of natural herbal remedies used in the treatment of common radiation reactions, as well as providing a marked reduction in the severity of radiation injuries when using for a long time and the acceleration of reparative post-radiation processes in the skin and mucous membranes.To solve the problem in the method of obtaining funds with radioprotective activity by mixing lichen with auxiliary substances - lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup, in the following ratio, wt.h.:
Lichen - 50
Lactose - 25
Starch - 25.We have developed the structure meets the requirements predyavlyaemie, flowability, hygroscopicity, filling mass.Revealed technological properties (or characteristics) describe the product as a means of preserving the constancy of composition, trade dress, as well as pharmacological effect during the entire time of use.Example 1.0.1 kg of vegetable raw materials lichen ground in a mill to a particle size diameter of 0.5 mm (sieve of 0.5 N GOST 214-83). Sifted powdered lichen, lactose in amounts of 0.05 kg and starch in the amount of 0.05 kg mixed in a porcelain mortar until a homogeneous mass.To a homogeneous mixture of the components of the chemical glass carefully add a 64% solution of sugar syrup in the number 0,160 l to education uravnoveshennoi mass, compression forming a lump, crumbling under pressure and not sticking to the glove.Moist mass is forced through a sieve with openings of 3 mm Get wet granules in the number 0,402 kg Wet granules are scattered on enamel tray and dried in a laboratory drying Cabinet at a temperature of 40oC with periodic mixing to a moisture content of 3,91%. Get 0,342 kg of granulate. The dried granulate is sieved che the possible number of small particles. Got 0,336 kg of finished product. The output on usninovoy acid is 92,86% of the finished granules.Radioprotective effect of granules developed lichen composition was evaluated according to the requirements of the normative document "guidelines for experimental and clinical study of radioprotective recipes" (1982). In particular, when determining the radioprotective action of Prime importance are changes in hematological parameters, the morphofunctional state of the mucous membranes of the gastrointestinal tract and changes in the weights of bodies involved in the restoration of cellular homeostasis of the blood system. On this basis have been used generally accepted methods blood tests (4). Assessment of the condition of the mucous membrane of the stomach was performed on the integral indicator - index Pauls (6).The experiments were carried out on outbred rats-males with initial mass 180,010,0, Animals were irradiated simultaneously on the remote machine "agate". The radiation source Co60activity of 1.84 1014Bq, power source 0,010100 Gy/s, field exposure 2020 (RIP = 75). Specific (radioprotective action of granules of lichen were evaluated under irradiation of animals is tava was added to the feed 1 time per day at the rate of 1 g/kg animal mass 15 days before and 30 days after irradiation. After 30 days of exposure in animals has produced a blood sample for analysis and then immediately killed by decapitation.The results of the experiments are presented in table. 1, 2.At the opening of observed pathological changes of gastric mucosa in animals of the control group: the relief of stomach was smooth, watched sharply pronounced hyperemia of the gastric fundus, multiple visible point of hemorrhage of the gastric mucosa. Liver and spleen were enlarged, marked involution of the thymus.Animals treated granules lichen, relief of stomach was partially smoothed, the destruction of the mucosa were noted. The liver and spleen within the physiological norm, the involution of the thymus moderate (table. 2).The results showed that exchange rate applying granules of lichen in the form of feed additives in the dose has a radioprotective effect: provides 100% survival of animals in the control group were killed 30%), retains and supports the functional state of organs (liver, spleen, thymus) (table. 2) protects the cellular composition of peripheral blood (table. 1), gastric mucosa.Sources detail the EB: Naukova Dumka, 1989. - 264 S.2. Goncharenko E. N. , Kudryashov Y. B. Radioprotective means of natural origin. //Successes of modern biology. - 1991. - So 3, vol. 2 - S. 302-316.3. Alexey Zhurbin, E. A., Chuchalin A. B. Hematology Radiation. - M.: Medicine, 1989.4. Laboratory methods in the clinic. //Menshikov Centuries, Delektorskaya L. N., Zolotnitskaya R. P. and others - M.: Medicine. - 1987. - S. 3675. Methodological guidelines for experimental and clinical study of radioprotective recipes. - M - 1982.6. Obolentseva, Century, Haji L. I. Influence of some flavonoid compounds on the formation of experimental gastric ulcers in rats. //Bull. the experimental. biology and medicine. - 1964. - N 9. - S. 86-88.7. Prospects radioprotective application of lichen acids. /Baraboi C. A., Maksimov, O. B., Krivoshekova O. E., Stepanenko HP, Mishchenko, N. P. //Proc. Dokl. fifth all-Union Symposium on phenolic compounds. - Tallinn, 1987. - S. 14-15.8. Preliminary results of the application of the phenolic drug lichens for the treatment of local complications of radiotherapy and combined therapy of malignant tumors. /Alexandrov S. C., Baraboi C. A., Krivoshekova O. E. , O. Maksimov B., Procyk C. S., Stepanenko HP //Proc. Nguyen Lien Huong, Kudryashov Y. B. Modification of radiation injury of animals by an aqueous extract of Hypericum perforatum L. 1 Message. //Biological science. - 1992, - N 4. - C. 7.0 The method of receiving means having a radioprotective effect, on the basis of lichen, characterized in that the lichen mixed with auxiliary agents, lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup in the following ratio, wt.h.:
Lichen - 50
Lactose - 25
Starch - 25s
< / BR>in which R1and R2each independently H, lower alkyl, lower alkenyl, phenyl, phenylalkyl, pyridyl or imidazolyl, and each of the groups lower alkyl, lower alkenyl, phenyl, phenylalkyl is optional from 1 to 5 substituents selected from carboxy, carbamoyl, cyano and hydroxy-group; or the group-Y - R2where Y is a carbonyl, thiocarbonyl or sulfonyl, and R3- H, lower alkyl, trifluoromethyl, phenyl, lower alkoxy, hydrazino, amino, phenylamino, carbarnoyl or Peregrina group, the lower alkyl or phenyl group are optionally 1 to 4 substituents such as hydroxy-group adjacent to the hydroxy-group-protected alkalinous group, carboxy or cyano, or R1and R2taken together with the nitrogen atom to which they are attached, form a piperazinilnom or pyrrolidinyloxy the group may hydroxylamino lower alkyl group, G - pentasa or hexana group, X1and X2independently H or halogen, OH, lower alkoxy - or benzyloxy
SUBSTANCE: method involves carrying out hernia removal in intralaminar way. Posterior longitudinal ligament defect is covered with Tacho-Comb plate after having done disk cavity curettage. Subcutaneous fat fragment on feeding pedicle is brought to dorsal surface of radix and dural sac.
EFFECT: enhanced effectiveness of treatment; reduced risk of traumatic complications.
FIELD: medicine, obstetrics, gynecology.
SUBSTANCE: at the background of therapy conducted one should introduce derinate immunomodulator into the body of pregnant woman additionally nasally per 1-2 drops of 0.25%-solution into each nasal canal 5-8 times daily for 3-5 d and - parenterally per 5.0 ml of 1.5%-solution once daily for 3-8 d along with preparation that improves microcirculation and along with antioxidant at a certain sequence, moreover, derinate should be introduced 30-40 min after application of microcirculation-improving preparation, and antioxidant - 20-30 min after derinate's introduction. The present innovation favors decreased edemas, decreased body weight, stabilization of Macluer-Aldrich test that in its turn enables to avoid perinatal losses, decrease the risk for the development of fetoplacental insufficiency and intrauterine fetal infection.
EFFECT: higher efficiency of therapy.
1 ex, 2 tbl
FIELD: medicine, otolaryngology.
SUBSTANCE: the present innovation deals with introducing neomycin sulfate antibiotic in granules prepared by the following technique. Tablet of neomycin sulfate 1.0g should be put into a vial with 100 ml distilled water till tablet's decomposition. Then vial's content should be shaken and kept till suspension sedimentation. In a day one should take 1 ml of supernatant liquid to be put into another vial and diluted with distilled water at 1:100 ratio. This procedure should be repeated 4 times more, moreover, during the last procedure one should apply alcohol for dilution. Then one should transfer the drop of alcoholic solution into a vial with granules out of milk sugar to then shaken and kept open for 1 d till granules" drying up. The suggested preparation should be applied per 1 granule under the tongue, moreover, multiplicity and duration of the above-suggested intake should be matched individually by patient's sensitivity and obtaining the clinic effect. The method enables to improve the value of tonic threshold audiometry by about 30-50 dB, decrease perception threshold of vocal range frequencies and widen the range towards high frequencies.
EFFECT: higher efficiency of therapy.
FIELD: medicine, oncology.
SUBSTANCE: invention relates to a method for treatment of chronic lympholeukosis. Method involves intravenous drop and jet administration of antitumor chemopreparations and carrying out the autochemotherapy. At the 1-st and 8-th day of treatment cyclophosphan in the dose 750 mg/m2, vincristine in the dose 1.4 mg/m2 and doxorubicin in the dose 30 mg/m2 incubated with 200 ml of autoblood are administrated to patients. From the 1-st to 14-th day of treatment prednisolone is used every day in the therapeutic dose. The treatment course is repeated in 30-35 days depending on blood indices and patient state. The total treatment of courses is 4-5. Method provides reducing cardiotoxicity of doxorubicin and cumulative toxicity of chemopreparations that allows carrying out administration of antitumor chemopreparations in the full volume to patients of elderly age groups.
EFFECT: improved method for treatment.
FIELD: medicine, oncohematology.
SUBSTANCE: the present innovation deals with treating elderly patients with chronic lympholeukosis accompanied with cardiovascular failure. The method deals with applying chemopreparations and cytoprotector. Moreover, 1 wk before the onset of chemotherapeutic therapy one should prescribe preductal at the dosage of 105 mg daily. At this background one should sample blood out of elbow vein at the volume of 200 ml into a vial with glugicir to centrifuge it, isolate plasma, divide into two portions, add into the 1st vial - cyclophosphan 600-800 mg/sq. m, vincristin 1.4 mg/sq. m, into the 2nd vial - adriamycin 50 mg/sq. m to be incubated for 30 min at 37 C and intravenously injected by drops for patients. Simultaneously, the intake of prednisolone should be prescribed at the dosage of 60 mg/sq. m since the 1st d and during the next 5 d and preductal at the dosage of 105 mg daily during a week, and then 2 wk more at the dosage of 60 mg daily. All the procedures should be repeated in above-mentioned sequence 4-6 times. The method enables to decrease toxic manifestations of chemotherapy while applying adequate dosages of cytostatics, anthracycline antibiotics, among them, at no great manifestations of their toxicity due to preductal's cardioprotective action.
EFFECT: higher efficiency of therapy.
1 ex, 5 tbl
FIELD: medicine, immunology, nucleic acids.
SUBSTANCE: invention relates to a method for stimulation of the immune response using nucleic acids-containing immunostimulating compositions, oligonucleotide-containing composition and to a method for treatment or prophylaxis of allergy or asthma. For stimulation of the immune response thymidine-enriched nucleic acid comprising poly-T sequences and/or comprising above 60% of thymidine-containing nucleotide residues is administrated. Invention provides the development of new method for stimulation of the immune response due to administration of the proposed immunostimulating nucleic acid.
EFFECT: valuable medicinal properties of nucleic acid.
27 cl, 12 dwg, 12 ex
FIELD: veterinary science.
SUBSTANCE: the suggested method should be performed under conditions of experimental modeling dystrophic process due to intraosseous introduction of glucosamine hydrochloride at the dosage of 15-25 mg/kg 1-2 times weekly during a month. The method provides high local concentrations of medicinal preparation, at its steady entering the circulation by leaving aside hepatic barrier and, as a result, optimization of chondroprotector action of glucosamine hydrochloride and better treatment of articular alterations induced in the course of an experiment in animals.
EFFECT: higher efficiency of correction.
8 dwg, 1 ex