The method of obtaining funds with radioprotective activity

 

(57) Abstract:

The invention relates to the field of pharmacy and concerns receiving means having a radioprotective effect. Mixed lichens, as well as excipients lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup in the following ratio, wt.: lichen 50, lactose 25, starch 25. The method allows to extend the range of natural plant materials to obtain protective remedies. table 2.

The invention relates to the field of pharmacy and relates to a method of receiving means having a radioprotective effect.

Due to the increased threat of increasing radiation pollution, as well as features of chronic radiation sickness and the ability of the damaging effects of radiation to cumulative effects (the summation), the ongoing risk of exposure of radionuclides in the body and their accumulation in the tissues, especially important is the use of radioprotective drugs that do not have side effects and harmless with long-term use. This problem is particularly acute for residents of the areas affected by the accident at Cannabalizing action occupy a special place drugs of natural origin as a means of prevention and treatment of radiation injuries, having several advantages over synthetic analogues.

First of all natural radioprotectors promising under the action of small doses of radiation and chronic exposure, low toxicity and capable of long-term save activity when administered orally.

Synthetic drugs, such as selenium or sulfur, used most often with simultaneous irradiation with a powerful dose and is not used for chronic radiation sickness, because these substances can accumulate, leading to their toxic effect on the body (1).

Among the natural radioprotective tools there are tools like animal poisons, extragenic, immunomodulators, isolated from different tissues) and plant origin (photoadaptive, polysaccharides, alkaloids), are able to exert a specific action and to improve the overall nonspecific resistance of the organism (2, 7, 8). However, some of them, for example, an extract of St. John's wort (Hypericum perforatum L.), according to the latest data (9), which has a radioprotective effect, has limitations apply burns, accompanying radiation damage, because it is characterized by slowing of the healing process.

Main mA the teachings of the finished product.

Upon receipt of the extracts of the starting substance (e.g., plant material) is subjected to prolonged heating, which can lead to the loss of biologically active substances. For example, when the extraction of plant raw materials containing such compounds as iridoid, when heated to temperatures above 40oC lost a significant portion of biologically active substances.

The objective of the proposed technical solution is the expansion of the range of natural herbal remedies used in the treatment of common radiation reactions, as well as providing a marked reduction in the severity of radiation injuries when using for a long time and the acceleration of reparative post-radiation processes in the skin and mucous membranes.

To solve the problem in the method of obtaining funds with radioprotective activity by mixing lichen with auxiliary substances - lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup, in the following ratio, wt.h.:

Lichen - 50

Lactose - 25

Starch - 25.

We have developed the structure meets the requirements predyavlyaemie, flowability, hygroscopicity, filling mass.

Revealed technological properties (or characteristics) describe the product as a means of preserving the constancy of composition, trade dress, as well as pharmacological effect during the entire time of use.

Example 1.

0.1 kg of vegetable raw materials lichen ground in a mill to a particle size diameter of 0.5 mm (sieve of 0.5 N GOST 214-83). Sifted powdered lichen, lactose in amounts of 0.05 kg and starch in the amount of 0.05 kg mixed in a porcelain mortar until a homogeneous mass.

To a homogeneous mixture of the components of the chemical glass carefully add a 64% solution of sugar syrup in the number 0,160 l to education uravnoveshennoi mass, compression forming a lump, crumbling under pressure and not sticking to the glove.

Moist mass is forced through a sieve with openings of 3 mm Get wet granules in the number 0,402 kg Wet granules are scattered on enamel tray and dried in a laboratory drying Cabinet at a temperature of 40oC with periodic mixing to a moisture content of 3,91%. Get 0,342 kg of granulate. The dried granulate is sieved che the possible number of small particles. Got 0,336 kg of finished product. The output on usninovoy acid is 92,86% of the finished granules.

Radioprotective effect of granules developed lichen composition was evaluated according to the requirements of the normative document "guidelines for experimental and clinical study of radioprotective recipes" (1982). In particular, when determining the radioprotective action of Prime importance are changes in hematological parameters, the morphofunctional state of the mucous membranes of the gastrointestinal tract and changes in the weights of bodies involved in the restoration of cellular homeostasis of the blood system. On this basis have been used generally accepted methods blood tests (4). Assessment of the condition of the mucous membrane of the stomach was performed on the integral indicator - index Pauls (6).

The experiments were carried out on outbred rats-males with initial mass 180,010,0, Animals were irradiated simultaneously on the remote machine "agate". The radiation source Co60activity of 1.84 1014Bq, power source 0,010100 Gy/s, field exposure 2020 (RIP = 75). Specific (radioprotective action of granules of lichen were evaluated under irradiation of animals is tava was added to the feed 1 time per day at the rate of 1 g/kg animal mass 15 days before and 30 days after irradiation. After 30 days of exposure in animals has produced a blood sample for analysis and then immediately killed by decapitation.

The results of the experiments are presented in table. 1, 2.

At the opening of observed pathological changes of gastric mucosa in animals of the control group: the relief of stomach was smooth, watched sharply pronounced hyperemia of the gastric fundus, multiple visible point of hemorrhage of the gastric mucosa. Liver and spleen were enlarged, marked involution of the thymus.

Animals treated granules lichen, relief of stomach was partially smoothed, the destruction of the mucosa were noted. The liver and spleen within the physiological norm, the involution of the thymus moderate (table. 2).

The results showed that exchange rate applying granules of lichen in the form of feed additives in the dose has a radioprotective effect: provides 100% survival of animals in the control group were killed 30%), retains and supports the functional state of organs (liver, spleen, thymus) (table. 2) protects the cellular composition of peripheral blood (table. 1), gastric mucosa.

Sources detail the EB: Naukova Dumka, 1989. - 264 S.

2. Goncharenko E. N. , Kudryashov Y. B. Radioprotective means of natural origin. //Successes of modern biology. - 1991. - So 3, vol. 2 - S. 302-316.

3. Alexey Zhurbin, E. A., Chuchalin A. B. Hematology Radiation. - M.: Medicine, 1989.

4. Laboratory methods in the clinic. //Menshikov Centuries, Delektorskaya L. N., Zolotnitskaya R. P. and others - M.: Medicine. - 1987. - S. 367

5. Methodological guidelines for experimental and clinical study of radioprotective recipes. - M - 1982.

6. Obolentseva, Century, Haji L. I. Influence of some flavonoid compounds on the formation of experimental gastric ulcers in rats. //Bull. the experimental. biology and medicine. - 1964. - N 9. - S. 86-88.

7. Prospects radioprotective application of lichen acids. /Baraboi C. A., Maksimov, O. B., Krivoshekova O. E., Stepanenko HP, Mishchenko, N. P. //Proc. Dokl. fifth all-Union Symposium on phenolic compounds. - Tallinn, 1987. - S. 14-15.

8. Preliminary results of the application of the phenolic drug lichens for the treatment of local complications of radiotherapy and combined therapy of malignant tumors. /Alexandrov S. C., Baraboi C. A., Krivoshekova O. E. , O. Maksimov B., Procyk C. S., Stepanenko HP //Proc. Nguyen Lien Huong, Kudryashov Y. B. Modification of radiation injury of animals by an aqueous extract of Hypericum perforatum L. 1 Message. //Biological science. - 1992, - N 4. - C. 7.0

The method of receiving means having a radioprotective effect, on the basis of lichen, characterized in that the lichen mixed with auxiliary agents, lactose and starch, as humidifiers add 0,16 l 64% solution of sugar syrup in the following ratio, wt.h.:

Lichen - 50

Lactose - 25

Starch - 25s

 

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