The drug "tevtropin" for disinfection of objects sanitary supervision

 

(57) Abstract:

The invention relates to the field of veterinary medicine and the development of a technologically advanced and environmentally friendly disinfectant - atropina (C8H16N4) and its use for disinfection of pathogenic viruses and bacteria premises, equipment, implements, utensils, tools, instruments and other objects of sanitary supervision. The invention consists in that for disinfection (disinfection) drug use, Tevtropin with virucidal and bactericidal action, low toxicity and chemical aggressiveness. Disinfection is performed with 0.1 - 5%-tion aqueous solutions of the preparation method of irrigation or aerosol. Tevtropin has no toxic effect on animals and birds, is synthesized from locally produced raw materials and stores disinfectant properties in aqueous solutions of not less than 120 days. table 4.

The invention relates to the field of veterinary medicine and the development of a drug for disinfection (disinfection) from pathogenic viruses and bacteria premises, equipment, implements, utensils, tools, instruments, solutions and environmentally corrosive be the technological and storage facilities, in research and medical institutions, enterprises of biological and food industry. Disinfection can be done by sprinkling or spraying method, including in the presence of animals.

It is known that for disinfection method of irrigation used various disinfectants, including: 10-20% suspension of hydrated lime, 5% hot solution of sodium carbonate, 3% hot emulsion disinfection creoline, 2% hot emulsion xylonite, 5% emulsion naphtalite, 3% hot solution caustification adaptarnos mixture, 1% solution of formaldehyde, 2% sodium hydroxide solution, bleach solution, containing 2% active chlorine 5%th solution of bleach and others, at the rate of 1 - 2 l 1 m2decontaminate the surface depending on its density. The hot solution should have a temperature of not lower than 70oC, and a solution of soda ash at least 90oC. If the disinfection is carried out by aerosol method used: 6% solution of hydrogen peroxide, formalin-cialisbuy mixture or formalin (36 - 40% aqueous solution of formaldehyde) (Veterinary legislation. -M.: Kolos, 1973).

However, it is known that in many e as chlorine, formaldehyde, atomic oxygen, etc. that can create an explosive mixture, lead to emergency situations and the poisoning of animals and people. This makes it dangerous and almost impossible to carry out disinfection in the presence of animals. Solutions such disinfectants are electrolytes, causing corrosion of metal surfaces, assemblies, parts and components. Aggressive gases produced during disinfection, cause discoloration of pigments and changes in the structure of organic polymers, causing damage to objects that have protective or insulating coating of paint and polymeric organic compounds.

The proposed drug eliminates the above mentioned disadvantages.

Among described in domestic and foreign literature, as also indicated in the "Veterinary legislation" disinfectants failed to find even close to the chemical structure of the analog prototype.

The goal is to develop environmentally friendly disinfectant with bactericidal and virucidal effect, and a method for its use for disinfection of livestock, medical, technological and warehouse space and objects.

The essence of the proposed concentrations, characteristic: the lack of toxicity in humans and animals, no odor and irritant to skin and mucous membranes, no corrosive action on metals and aggressive actions on the paint and synthetic polymeric materials and coatings, high solubility in water and stability of aqueous solutions in a wide range of temperatures, a pronounced bactericidal and virucidal activity in the temperature range from +4 to +40oC, the possibility of disinfection in the presence of animals and birds.

The specified requirements are met by aqueous solutions of atropina. Preparation of Tevtropin first synthesized at the Moscow Institute of fine chemical technology doctor of chemical Sciences Kuznetsov, A. I., which is the coauthor of the invention. Synthesis of atropina is carried out according to the following scheme

< / BR>
Interaction of ethylene diamine with formaldehyde in an aqueous medium at a temperature of 10-12oC received tetramethylenedisulfotetramine (tevtropin). The structure of the obtained compound is confirmed by elemental analysis, NMR1H and N14the reaction course was monitored by TLC.

In a round bottom flask with a capacity of 1000 ml place >C, kept at this temperature for 4 hours, add KOH or NaOH, the precipitate is filtered off and crystallized. Get 168 g (70%) tetramethylenedisulfotetramine (atropina).

Found, %: C - 57,0; H - 9,5; N - 33,2.

Calculated, %: C - 57,1; H - 9,5: N - 33,3.

Melting point 196-201oC.

Preparation of Tevtropin structural formula

< / BR>
Chemical name - tetramethylenedisulfotetramine;

- 1,8,3,6-vandommelen-1,3,6,8-tetraazacyclododecane;

- 1,3,6,8-tetraazatricyclo/4.4.1.1.3.8./dodecan.

Empirical formula C8H16N4.

Molecular weight of 168.

Physical condition - crystals of white and yellow, soluble in water.

The effectiveness of the proposed method proved experimentally in the laboratory and confirmed by production testing.

Virucidal activity of atropina studied with DNA - and RNA-containing viruses that cause: African swine fever (ASF), classical swine fever (CSF), Aujeszky's disease (AD), foot and mouth disease, vesicular exanthema of swine (WPP), vesicular disease swine (WBS), bluetongue (BT), Teschen disease (BT), bird flu (SE) and bolezn is aparatow", M., 1984. Virucidal activity was determined by the decrease in the titre of virus in experimental samples relative to the control after 18-20 h of contact of a test sample with a solution of atropina and expressed in lg AI50(hemadsorbing units), lg TCD50(tissue cytopathic doses), lg FIGHT50(plaque-forming units), lg EDS50(embryonic lethal doses).

In table. 1 shows the results of determining the inactivating action of atropina on the number of DNA - and RNA-containing viruses, the causative agents of animal diseases.

From the data presented in table. 1, it is seen that tevtropin in a concentration of 0.1% (1000 g/ml) for 18 to 20 h of exposure at 37oC fully inaktiverad samples as DNA-containing, RNA-containing viruses, pathogens dangerous diseases of animals and birds.

Antibacterial activity of atropina studied with the causative agents of anthrax in spore and vegetative forms, colibacillosis, mycoplasmosis and Listeria according to the "Methodological guidance on the definition of sensitivity to antibiotics of infectious diseases of agricultural animals", M., 1972.

The minimum bactericidal concentration (MBC) was determined by the results of a comparative titration of conm broth (BCH) followed by plating on mastopathy agar (MPA). In table. 2 presents the results of these studies.

From the data table.2 shows that tevtropin at a concentration of 0.65% (6.5 mg/ml) for 24 h inactivate spore forms of anthrax, and at a concentration of 0.06% (0.6 mg/ml) - pathogens colibacillosis, mycoplasmosis and listeriosis.

Disinfectant action of atropina was tested on test objects, imitating objects livestock buildings, stekloposudy and overalls.

On the test objects made of wood, glass, steel, brick, tile, concrete, ceramic floor tiles caused the suspension of anthrax spores at the rate of 50 million spores/ml) and was dried in air. After that, the test objects were placed in an incubator at +37oC and sprayed from electroslag generator 30 ml of a 5% solution of atropina, i.e. a rate of 60 ml/m3. After 18-20 h from the surface of the test object sterile Vaticani did the swabs in the BCH, followed by seeding in a Petri dish filled with MPA. After 3-4 days. incubation in Petri dishes with sowing swabs with experienced test objects colony growth of the pathogen anthrax were not observed, whereas in the cups with sowing swabs from the test-objects, not treated with a solution of atropina, and water, said solid growth number is terylene test objects was applied at 0.25 ml vaccinated blood and 0.2 g of sterile pig manure, thoroughly mixed with a spatula and evenly distributed across the surface of the test object. After drying (2-3 h at 20-23oC) pilot test objects evenly watered examinees solutions (2,3 and 5% indigenous) tevtropin rate of 1 l/m2. For control test objects instead of the solution of atropina inflicted the same amount of water. After 18-20 h of incubation at 20-23oC with test objects scraped vaccinated material, suspended it in 5 ml medium Hanks, were extracted for 30 min at 20-23oC and osvetleni by centrifugation at 3000g for 30 min the Supernatant fluid was infected cell culture bone marrow pigs (CCMS) with the following two passages and intact pigs (bioassays). The results of the verification of the completeness of decontamination test objects contaminated by the virus, are presented in table.3.

From the data table.3 shows that 5 and 3% solutions of atropina disinfect contaminated virulent virus test objects that mimic fragments of livestock premises.

Stekloposudy contaminated by the virus, they were immersed in 2, 3 and 5% solutions of atropina (in the ratio of 1/10 by volume) for 18 to 20 h at 20-23oC, and then used rasana size 15X15 cm and rubber gloves, contaminated vaccinated blood, was immersed in 2, 3 and 5% solutions of atropina (in the ratio of 1/10 by volume) for 18-20 h at 20-23oC. After processing, the material was removed from the disinfecting solution, were crushed in a porcelain mortar, transferred to Hanks solution, was extracted under stirring for 30 min, and extracting the solution was infected CCMS and pigs. These studies were tested solutions tevtropin stored in 15, 20 and 120 day (time of observation) at 20-23oC. quality control of the used water.

The results of the assessment of completeness of decontamination of glassware, fabric and rubber materials contaminated by the virus, are presented in table.4.

From the data table. 4 shows that 2, 3 and 5% solutions of atropina kept up to 120 days (observation period) for 18-20 h fully disinfect stekloposudy, fabric and rubber materials. The results were confirmed by bioassays on the pigs, which are not contacted within 30 days, whereas animals injected materials control (untreated Tiotropium test objects), fell ill with fever in the acute form, and fell to 6-8 days, the titer of virus in the blood of dead pigs was in the range of 6.5 to 8.0 lg AI50/ml. Commission Act test, a confirmation is lethal dose (EDS50for developing chick embryos is 364 g/kg and the LD50(lethal doses) for white mice 375 ml/kg after subcutaneous administration. Tevtropin did not show teratogenic and embryotoxic. When spray applied at a rate of 40 ml of 10 and 25%-aqueous solution of 1 m3and exposure for 60 min, tevtropin had no toxic effect on the chickens and pigs. Acts of Commission and production tests are available.

Thus, the proposed drug Tevtropin for disinfection (disinfection) of objects sanitary inspection simple and industrially applicable in animal husbandry, medical and research institutions, technology, engineering, manufacturing and warehouse facilities, businesses biological and food industry. Tevtropin domestic production is synthesized from locally produced raw materials and stores disinfectant properties in aqueous solutions of not less than 120 days (observation period).

Connection tetramethylenedisulfotetramine structural formula

< / BR>
having virucidal and bactericidal action for disinfection of objects of sanitary supervision.

 

Same patents:

The invention relates to the production of antimicrobial agents, in particular, can be used for disinfection treatment, prevent the formation of fungi and other undesirable microorganisms in the premises, equipment, food industry, veterinary medicine, medicine, can also be used to protect foods, as additives in paints, varnishes, vodoemulsionnye compositions
The invention relates to medicine and can be used in the practice of surgery and traumatology
The invention relates to the field of sanitation and applies disinfectants
Antiseptic // 2118174
The invention relates to the cleaning and sterilizing medical instrument, in particular endoscopes, and can be used in medical institutions using endoscopes to diagnose and/or treat

The invention relates to compositions and method to quickly and efficiently determine whether it's been functional component, such as catalase, transparent solution, such as hydrogen peroxide solution, by mixing with the specified functional component of an effective amount of vitamin B-12 so that when adding the mixture into a clear solution, this solution took the pink color

The invention relates to medicine and can be used in clinical medicine as antimicrobial, disinfectant and sterilizing agent, used as a skin antiseptic for treatment of surgical and injectable fields elbow bends donors

The invention relates to medical engineering, in particular to a device for cleaning and sterilization of endoscopes, and can be used in medical institutions using endoscopes to diagnose and/or treat

The invention relates to the production of antimicrobial agents, in particular, can be used for disinfection treatment, prevent the formation of fungi and other undesirable microorganisms in the premises, equipment, food industry, veterinary medicine, medicine, can also be used to protect foods, as additives in paints, varnishes, vodoemulsionnye compositions

The invention relates to medicine and can be used in surgery, obstetrics, cardioangiography etc
The invention relates to medicine and can be used in the practice of surgery and traumatology
The invention relates to the field of sanitation and applies disinfectants

The invention relates to biotechnology and can be used in medicine
Antiseptic // 2118174

Disinfectant for // 2117491
The invention relates to chloractive drugs used for disinfection
The way the animals // 2115434

The invention relates to the development of methods of disposal of sewage in which the neutralized effluent can be used in agriculture, in the production of organo-mineral fertilizers, etc
The invention relates to medicine, namely dezinficiruy-detergents intended for disinfection of surfaces in premises, sanitary-technical equipment, linens, Housewares, medical devices, and for sterilization of medical products
Up!