The method of recovering damaged corneal endothelium

 

(57) Abstract:

The invention relates to ophthalmology and is designed to repair damaged corneal endothelium. In front of the camera enter solution, consisting of a complex of heparin with copper ions in the buffer saline solution at the following ratio of components in 1 ml buffer saline at pH 7.4 heparin 20 to 150%, of copper ions 0,001 - 0,003 mg Solution you can enter the injection and by phonophoresis. The result is activation of proliferation of corneal endothelium by placing the stimulator of proliferation of endothelial - fibroblast fact growth contained in descemets membrane from an inactive form to an active and increasing mobility of the endothelium. 2 C.p. f-crystals, 2 Il.

The invention relates to the field of medicine and more specifically to ophthalmology.

It is known that the possibility of mitotic division in the endothelium (rear epithelium) of the cornea of a person is very limited. Regeneration of the endothelium occurs as a result of hypertrophy and expansion of endothelial cells and their migration. By reducing the density of endothelial cells of the cornea (less than 1000 cells/mm2as a result of accidental injury or operating, or illness is vosstanovleniya damaged endothelium by introducing purified fibroblast growth factor in the anterior chamber of the eye. However, the author obtained positive results only in experiment (Exp. Eyl. Res. 1987, 45 805-811).

The disadvantage of the proposed method is the necessity to use for insertion into the eye cavity substances protein as fibroblast growth factor is a polypeptide. This can result in allergic reaction.

Object of the invention is to provide a method for recovery of damaged corneal endothelium.

The technical result of the invention is the activation of proliferation of corneal endothelium as a result of translation of a stimulator of proliferation of endothelial - fibroblast growth factor contained in descemetocele the membrane from the inactive form to the active and increase mobility of the endothelium.

The technical result is achieved by the fact that in the known method of recovering damaged corneal endothelium according to the invention in front of the camera enter solution, consisting of a complex of heparin with copper ions in a balanced salt solution at the following ratio of components in 1 ml of balanced salt buffer solution at pH 7.4 heparin 20-150%

Cu++- 0.001-0.003 mg

Engl is in the culture medium, containing that we offer the drug, then after 7 days in contrast to the control of the defect of the endothelium in the damage zone is not defined, and the density of endothelial cells in this zone is high enough 3400-2800 cells/mm2and corresponds to the norm.

In Fig.1 is seen a high enough density of endothelial cells in the damage zone after placing the cornea in kulturalny environment with the drug. In Fig.2 - in the damage zone detected a significant defect in endothelium.

The drug is administered after the operation cataract extraction with IOL implantation in the early postoperative period when keratopathy, with the development of the initial (stage I-II) endothelial-epitheliale dystrophy, as well as by reducing the density of endothelial cells in patients with artiphakia and risk of endothelial-epithelial dystrophy.

After cataract extraction with IOL implantation, the drug is injected in front of the camera, filling her in amount of 0.2-0.3 ml, and in all other cases the method phonophoresis with the help of the device UST-307 in continuous mode for 10 minutes at the intensity of 0.2-0.3 W/cm2. In one session using 5 ml of the drug. Just spend 5 sessions.

Example 1. The patient P. the Diagnosis of immature s with IOL implantation model T-26 without complications. The operation was completed by the introduction in front of the camera 0.2 ml of the proposed drug. 3 months after the operation, the density of endothelial cells 2100-2200 cells/mm2. The cell loss was 4.6%.

Example 2. Patient M. Diagnosis: artiphakia, iris-clip lens implanted in 1980, the Density of endothelial cells gradually decreased to 1200-1300 kg/mm2. On the second eye artiphakia, endothelial-epithelial dystrophy. The course phonophoresis with the drug. At the end of treatment disappeared subjective complaints after 3 months PEK 1300-1450 cells/mm2.

1. The method of recovering damaged corneal endothelium, including the introduction of the solution into the anterior chamber, characterized in that as a solution using a solution consisting of a complex of heparin with copper ions in a salt buffer solution in the following ratio of components in 1 ml of saline buffer solution at pH 7.4

Heparin - 20-150 units

Cu++- 0,001-0,003 mg

2. The method according to p. 1, characterized in that after cataract extraction with IOL implantation anterior chamber is filled with a solution in a quantity of 0.2-0.3 mil

3. The method according to p. 1, characterized in that in the postoperative period when keratopathy, when the initial development is yunam mode for 10 minutes, when the intensity of 0.2-0.3 W/cm2and use 5 ml per session and spend 5 sessions.

 

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