The method of obtaining colored diagnosticum for the determination of antigens and antibodies of infectious and other diseases
(57) Abstract:Usage: in medicine, veterinary medicine, food industry for the determination of antigens and antibodies of infectious diseases and cancer, alcoholism, drug abuse, drug testing, AIDS. The inventive method of getting colored diagnosticum, including sensitization of latex - type core-shell, where the core - polystyrene latex sheath - copolymer of styrene and salts of zinc and copper unsaturated carboxylic acid at a mass ratio of links 1 : 0,3 - 3,0 : 0,3 - 3,0, moreover, the latex pre-incubated in buffer at pH 6.5 to 9.0 in the presence of 0.15 to 5.0 wt.h by weight of polymer of sodium dimethyldithiocarbamate within 1 to 3 months with subsequent sensitization at pH 7.0 - 9.0 and incubating at 37 - 56°C for 80 to 300 rpm, and then at 2 - 8oWith within 24 - 72 hours of 8 tablets. The invention relates to medicine, veterinary medicine and food industry, namely the method of producing diagnosticum for the determination of antigens and antibodies of infectious diseases and cancer, alcoholism, drug abuse, doping control and AIDS.Closest to the proposed method is a method of obtaining diagnosticum for operosa polystyrene latex sensibiliser antibodies in 0.1 M glycine buffer at pH 7.0, and then carry out incubation at 37oC for 2 to 8 h .Diagnosticum obtained by this method allows to detect HBsAg hepatitis B virus only at a concentration of 1 μg/ml of protein, it has a high enough frequency monopolistically reactions (30 - 40%). The specificity of such diagnosticum is expressed by the value 68,02 0,88. In addition, the resulting diagnosticum allows you to get the results of the study in 20 minutesThe purpose of the invention is improving the sensitivity and specificity of diagnosticum to proteins and organic substances.This objective is achieved in that in a method of producing colored diagnosticum for the determination of antigens and antibodies of infectious diseases, including sensitization of the modified polystyrene latex antibodies or antigens in a buffer solution at pH 7.0 and incubation sensitized latex, use a latex type core-shell, where the core - polystyrene latex sheath - copolymer of styrene and salts of copper and zinc unsaturated carboxylic acid at a mass ratio of 1 links : 0,3 - 3,0, with the specified latex pre-incubated in buffer pH of 6.5 - 9.0 in the presence of 0.15 to 5.0 wt.h. from the weight of the polymer is started when a 37 - 56oC within 24 - 72 hours Received diagnosticum can be used to determine HBsAg hepatitis B virus in a concentration of 1 PG/ml, hepatitis A concentration of 1 PG/MK, cancer antigens at a concentration of 10 PG/ml, protein doping control and alcoholism at a concentration of 50 PG/ml and serum of patients with AIDS, the virus-containing AIDS in known concentration. Diagnostics can be performed using serum, saliva and urine of the patient.The specificity of diagnosticum is 97,0 2,2.For diagnostic antigens and antibodies of infectious and other diseases used method of activated particles (MAH), namely, that the evaluation of the results of reactions performed according to the degree of activation of latex particles, visual or optical device. The degree of activation of the particles. Evaluation of the results of MAH,
+++ - large homogeneous flakes with a small amount of smaller to slightly turbid background, a positive result;
++++ big flakes in a fully transparent background, positive result;
++ - medium size particles in turbid background, equivocal result;
+ - fine grain particles in turbid background, negative result;
- - muteena latex type core-shell and diagnosticum is illustrated by the following examples.Example 1. With the purpose of obtaining a polystyrene latex was charged to the reactor 10 wt.h. styrene, 0.1 wt.h. of potassium persulfate, to 89.9 wt.h. water, and the reaction mixture thermostatic under stirring at 70oC until complete conversion of the monomer.For part (A) of the obtained polystyrene latex was added 1 wt.h. styrene by weight of the total polymer (A), 1.0 wt.h. ethoxylated Nonylphenol OP-7, 0.3 wt.h. methacrylate zinc, 0.5.h. of potassium persulfate. The latex is stirred until dissolution of all components within 15 - 20 minutes and thermostatic at 70oC for 2 h Then enter 0.5.h. of sodium dimethyldithiocarbamate in the form of a 1% aqueous solution, the latex is washed by dialysis against 20 volumes of distillate from water-soluble impurities.For part (B) of polystyrene latex was added 1 wt.h. styrene by weight of the total polymer (B), 0.3 wt.h. methacrylate copper (Cu2+), 1,0 wt.h. OP-7, 0.5. including potassium persulfate. The latex is stirred for 15 to 20 minutes and thermostatic at 70oC for 2 hours, the Latex is cooled and washed by dialysis. Part of the latexes (A and B) are mixed and used for diagnosticum.Latex is characterized by a particle size of 0.5 μm and adsorption saturation of the surface Cham, glycerine-glycine buffer solution, pH of which 6,5 within 1 month.Then the latex is diluted glycine buffer solution up to 2% concentration and mixed in a ratio of 1 : 1 with monoclonal antibodies in a buffer solution at pH 7.0. The resulting mixture was sequentially incubated in 0.01 M glycine solution at pH 7.0 for 80 min at 37oC then 24 hours at 2oC.The latex is also used for composite-based assays, for example, to different specific antigens of different cancers.The results of diagnostic tests are given in table. 1 - 8.Example 2. The latex was prepared as in example 1, but using 3.0 mass.h. methacrylate copper, 3.0 mass.h. methacrylate zinc and 5.0 wt.h. dimethylthiocarbamate sodium.The resulting latex incubated in 1 M glycerine-glycine buffer solution at pH 9.0 in 3 months. Then the concentration of the latex was adjusted with glycine buffer solution up to 2%, then mix equal volumes of 2% latex and monoclonal antibodies in 1 M glycine buffer solution at a pH of 9.0. Sensitized latex incubated for 300 min at 56oC, and then at the 8oC for 72 hours.The results of the diagnostic sleetgrout zinc and copper and 0.15 wt.h. of sodium dimethyldithiocarbamate.The results are shown in table. 1.Example 4. Get the latex of example 1, using 5 wt.h. the methacrylates of copper and zinc, sodium dimethyldithiocarbamate to enter failed, the latex was Smagulova. The results are shown in table. 1.Example 5. Get the latex of example 1, using 0.3 wt.h. acrylate, copper and zinc. The results of diagnostic tests are given in table. 1.Example 6. Get the latex of example 1, using 0.3 wt.h. itaconate copper and zinc. The results of diagnostic tests are given in table. 1. The method of obtaining colored diagnosticum for the determination of antigens and antibodies of infectious and other diseases, including sensitization of polystyrene latex antibodies and antigens in a buffer solution at pH 7 and incubation sensitized latex, characterized in that, in order to increase the sensitivity and specificity to proteins and organic substances, as latex uses latex type core - shell, where the core - polystyrene latex sheath - copolymer of styrene and salts of zinc and copper unsaturated carboxylic acid, in mass ratio units 1 : 0,3 - 3,0 : 0,3 - 3,0, paiticipate sodium for 1 3 months with subsequent sensitization at pH 7 to 9 and a first incubation at 37 - 56oWith over 80 to 300 rpm, and then at 2 - 8oWith within 24 - 72 hours
FIELD: polymer materials.
SUBSTANCE: invention relates to preparing aqueous cationic latexes with hollow polymeric particles serving as multifunctional additives used when preparing polymer compositions, paintwork materials, coatings (including paper coatings), and in other applications as white pigment and filler reducing density of material and internal stresses arising during formation of coatings or polymer materials. Process comprises at least three following stages: (A) preparing functional core copolymer, (B) preparing particles with core-shell morphology, and (C) ionization of functional groups of core copolymer. All process stages are carried out as a series of consecutive transformations in the same reactor without discharge of intermediate products. As monomer containing functional groups in stage A, vinylbenzyl chloride is used, and stage C represents amination of vinylbenzyl chloride units with tertiary aliphatic amines, pyridine, or derivatives of the latter.
EFFECT: improved and simplified technology and improved characteristics of cationic latex.
10 cl, 6 ex
SUBSTANCE: N (4-anilinophenyl)amide of alkenylsuccinic acid of general formula , where n=6-18 is used as a stabiliser of phenylenediamine type. The stabiliser is introduced into latex in amount 0.1-1.5 weight fractions per 100 weight fractions of rubber as aqueous alkaline liquor. While using the stabiliser of low content of ballast substance less than 3% and high content of fragments of phenylenediamine type, is suitable to be introduced into latex.
EFFECT: maintained effective stabilisation and eliminated odour nuisance.
4 tbl, 12 ex
SUBSTANCE: rubber is extracted from latex continuously by mixing latex with a coagulant. Consumption of coagulant is varied depending on the given turbidity value of serum (primary serum), which is maintained by the amount of coagulant fed. The given turbidity value of primary serum is adjusted depending on the turbidity of the serum released (secondary serum) towards the minimum consumption of coagulant to obtain minimum turbidity of the released serum.
EFFECT: method of controlling the coagulation process enables to reduce contamination of waste water through loss of partially coagulated latex with minimum consumption of coagulants.
2 dwg, 6 ex
SUBSTANCE: method of extracting butadiene-nitrile rubber from latex is carried out by feeding sodium sulphite or sodium bisulphite or sodium pyrosulphite in amount of 0.05-0.8 wt % per latex, into a stream of latex degassed beforehand, into which an antioxidant emulsion is also fed. Further, the stream of latex, containing sulphite, is directed into a coagulation apparatus into which aqueous sulphuric acid and an organic amine coagulant in form of a quaternary polymer ammonium salt are also simultaneously fed, said salt being selected from: polydimethyl diallyl ammonium chloride, a methacrylamide and dimethyl aminoethyl methacrylate hydrochloride copolymer, and polydiethyl aminoethyl methacrylate hydrochloride. The amount of the polymer ammonium salt varies from 0.2-0.5 wt %, per rubber, depending on content of leukanol content in latex in the range of 0.1-0.4 wt %, per rubber, at coagulation pH 3-6 and temperature 30-70°C.
EFFECT: invention improves environmental friendliness of the process, specifically reduces content of unreacted monomer - free acrylic acid nitrile, avoids excess synthetic coagulant and the amount of salt used for coagulation.
3 tbl, 7 ex
SUBSTANCE: nitrile rubber contains repeating units of at least one α,β-unsaturated nitrile and at least one conjugated diene and has ionic index in the range of 7-26 ppm×mol/g. The nitrile rubber is obtained by emulsion polymerisation. The obtained latex, which contains nitrile rubber, is coagulated and the coagulated nitrile rubber is washed. Polymerisation is carried out in the presence of at least one alkylthiol. Before coagulation, the pH of the latex is set to at least 6, and coagulation is then carried out in the presence of at least one salt of a monovalent metal. The latex coagulation temperature ranges from 60 to 90°C and the washing temperature ranges from 50 to 90°C. The obtained nitrile rubber is used to obtain curable mixtures which contain said rubber and at least one cross-linking agent. The curable mixtures are cured by moulding to obtain moulded articles.
EFFECT: nitrile rubber has excellent curing rate and exceptional properties of cured products.
26 cl, 7 tbl, 13 ex
SUBSTANCE: invention relates to nitrile rubber, method of its obtaining and products, obtained from it. Claimed nitrile rubber contains structure repeat units of, at least, one α,β- unsaturated nitrile and, at least, one conjugated diene, and has ion indicator in range 0-60 ppm×mole/g. Nitrile rubber is obtained by emulsion polymerisation. Obtained latex, which contains nitrile rubber, is subjected to coagulation, and then coagulated nitrile rubber is washed. Polymerisation is carried out in presence of, at least, one alkylthiolate. Before coagulation value of latex pH is set at level, at least, 6, and then is coagulated in presence of, at least, one magnesium salt. Temperature of latex before adding at least one salt of magnesium is set at value 45°C. Obtained nitrile rubber is applied for obtaining capable of vulcanisation mixtures, which contain claimed rubber, at least, one linking agent and, if necessary, additional target additives to rubbers. Capable of vulcanisation mixtures are vulcanised by casting with obtaining cast products.
EFFECT: claimed nitrile rubbers have exclusive speed of vulcanisation, as well as exclusive properties of vulcanisates.
24 cl, 13 tbl, 17 ex
SUBSTANCE: invention relates to a method of producing synthetic latex from rubber. The method comprises steps of: (a) emulsifying a binding substance containing rubber, which is dissolved in a suitable organic solvent, together with an aqueous surfactant solution to form an oil-in-water emulsion; (b) step-by-step reduction of content of solvent in the oil-in-water emulsion in two or more steps, resulting in formation of synthetic latex. Also described is a reactor with continuous mixing for removing the organic solvent from the oil-in-water emulsion, which contains rubber dissolved in the organic solvent.
EFFECT: reducing the amount of residual solvent to very low levels with high efficiency of the process.
19 cl, 2 dwg, 1 tbl, 11 ex