The method of producing diagnosticum for the determination of antigens and antibodies of infectious and other diseases
(57) Abstract:Usage: in medicine, veterinary medicine, food industry for the diagnosis of infectious diseases and cancer, alcoholism, drug abuse, drug testing, AIDS. The inventive method of producing diagnosticum, including sensitization of latex type core-shell, where the core - polystyrene latex sheath - copolymer of styrene and a salt of iron and/or cobalt and/or Nickel unsaturated carboxylic acid at a mass ratio of 1 links : 0.3 to 0.5, and the latex pre-incubated in buffer at pH 6.5 to 9.0 in the presence of peroxide, sensitization is carried out at pH 7.0 - 9.0 and incubated first at 37 - 56oWith over 80 to 300 rpm, and then at 2 - 8oWith within 24 - 72 hours of 8 tablets. The invention relates to medicine and veterinary medicine and food industry, and more specifically to a method for the diagnosticum for the determination of antigens and antibodies of infectious diseases, cancer, alcoholism, drug abuse and doping control.Currently, for the diagnosis of viral infections has received widespread the so-called indirect reaction, based on the method of activated particles. As dia is elficiency carriers of organic or inorganic nature. Such carriers are often erythrocytes, coal, bentonite clay containing A protein, a synthetic latex.A method of obtaining the diagnosticum for determination of viral antigens and meningococcal infections based latex media . In accordance with this method, 2% synthetic latex containing functional groups such as carboxyl, aldehyde, or epoxypropyl incubated in 0.1 M glycine buffer at pH 7.0 - 8.2 for 2 h at 37oC to obtain the target product.Get diagnosticum more stable, has a high activity and specificity and allows results in a shorter time.The closest is the way to get diagnosticum for the determination of antigens and antibodies and other infectious diseases, such as antigens, HBsAg hepatitis B virus, according to which polystyrene latex sensibiliser antibodies in 0.1 M glycine buffer, pH which to 7.0, and then carry out incubation at a temperature of 37oC for 2 to 8 hours .Diagnosticum received this and the above methods allows to detect HBsAg hepatitis B virus only in civicnet such diagnosticum is expressed by the value (68,01 + 0,88). In addition, the resulting diagnosticum allows you to get the results of the study in 20 minutesThe purpose of the invention is the increased sensitivity and specificity to proteins and organic substances.This objective is achieved in that in the method of producing diagnosticum for the determination of antigens and antibodies of infectious diseases, including sensitization of the modified polystyrene latex at pH 7.0 and incubation sensitized latex, use a latex type core-shell, where the core - polystyrene latex sheath - copolymer of styrene and metal salts and unsaturated carboxylic acid at a mass ratio of 1 links : 0.3 to 0.5, as the metal salt of unsaturated carboxylic acid using iron salts or cobalt and/or Nickel. The shell is 0.2 - 20% by weight of the whole particle.The specified latex pre-incubated in buffer at pH 6.5 to 9.0 in the presence of 0.5 - 0.7 wt.h. organic gidroperekisi within 1 to 3 months, followed by sensitization with pH 7.0 - 9.0 and incubating at 37 - 56oC for 80 to 300 rpm, and then at 2 - 8oC within 24 - 72 hours Received diagnosticum used to determine HBSAg hepatitis B virus in the concentration of the of thenov doping control and alcohol at a concentration of 50 PG/ml, as well as the AIDS virus in a known concentration contained in the serum of patients with AIDS. For diagnostic use serum, or urine, or saliva of the patient specificity of diagnosticum the 98.9 0,2. Received combined diagnosticum to different specific antigens of cancer, infectious and other diseases.For diagnostic antigens and antibodies of infectious and other diseases of the method of activating particles (MAH), namely, that the evaluation of the results of reactions carried out according to the degree of activation of latex particles, visual or optical device. Assessment of the MAH on the degree of activation of the particles is as follows:
4+ - large flakes of activated particles in a fully transparent background, positive result;
3+ is a large homogeneous grains of activated particles with a small amount of smaller to slightly turbid background, a positive result;
2+ - medium grain sizes of activated particles in turbid background, equivocal result;
1+ - small-activated particles in turbid background, negative result;
- - murky background, active particles, a negative result.The method of producing latex depoliticising latex was charged to the reactor 10 wt. including styrene, 0.1 wt.h. of potassium persulfate, to 89.9 wt.h. water and the reaction mixture thermostatic under stirring at 70oC until complete conversion of the monomer. Part A received polystyrene latex was added 1 wt.h. methacrylate iron, 0.5.h. of potassium persulfate, 1.0 wt.h. ethoxylated Nonylphenol OP-7. The latex is stirred until dissolution of all components within 15 - 20 minutes and thermostatic at 70oC for 2 h and Then injected with 0.1 wt. including sodium sulfate, the latex is cooled and washed by dialysis against 20 volumes of distillate from water-soluble impurities. The latex particles part A consist of two parts: the inner core is polystyrene, the outer shell is a copolymer of styrene and methacrylate iron at a mass ratio of 1 : 0.3 to. The mass ratio between the links of iron methacrylate and styrene, which is part of the core and shell is 0,297 - 100.Then the latex is diluted glycine buffer solution up to 2% concentration and mixed in a ratio of 1 : 1 with monoclonal antibodies in a buffer solution at pH 7.0. The resulting mixture was sequentially incubated in 0.01 M glycine solution at pH 7.0 for 80 min at 37oM, then 24 h at 2oC.The results of the diagnostic issuedata iron, 0.5.h. sodium sulfite and 0.7 wt.h. the gidroperekisi GEPCPB.10 ml of 6% aqueous latex incubated in 1 M glycerine-glycine buffer solution pH 9.0 in 3 months.Then the concentration of the latex was adjusted with glycine solution to a value of 2%, then mix equal volumes of 2% latex and monoclonal antibodies in 1 M glycine buffer solution at a pH of 9.0. Sensitized latex is then incubated at a pH of 9.0 during the first 5 h at 56oC, and then at the 8oC for 72 hThe results of diagnostic tests are given in table. 1 - 8.To determine the sensitivity of diagnosticum use serum AIDS patients, the serum of pregnant women, the antigens of the virus of hepatitis A and B and cancer.The sensitivity of the combined latex or antibody-based test-based assays (FRET) is shown in table. 7 and 5.Examples 3 and 4. Latex and diagnosticum prepared as in example 1, but using 0.5 wt.h. the gidroperekisi p-Mentana and tertbutylphenol (HPCUPS). The results of diagnostic tests are given in table 1.Examples 5 and 6. Latex and diagnosticum prepared as in example 1, but using methacrylate of cobalt elasticum prepared as in example 1, but using a 0.3 wt.h. acrylate iron and 0.3 wt.h. itaconate iron.The results of diagnostic tests are given in table. 1.Example 9. The latexes A and B prepared as in example 1, the latexes A and B separately subjected to sensitization and incubation in example 1, and then mixed. The results of diagnostic tests are given in table. 1.Example 10. Latex and diagnosticum prepared as in example 1, but using a 0.3 wt.h. and iron methacrylate and 0.2 wt.h. methacrylate cobalt. The results of diagnostic tests are given in table 1.Example 11.Latex and diagnosticum prepared as in example 1, but using 0.2 wt.h. the iron methacrylate and 0.3 wt.h. methacrylate Nickel. The results of diagnostic tests are given in table 1. The method of producing diagnosticum for the determination of antigens and antibodies of infectious and other diseases, including sensitization of polystyrene latex antibodies and antigens in a buffer solution at pH 7 and incubation sensitized latex, characterized in that, in order to increase the sensitivity and specificity to proteins, use a latex type core - shell, where the core - polystyrene latex, Obolon the ratio of parts of 1 : 0.3 to of 0.5, and the specified latex pre-incubated in buffer at 6.5 to 9.0 in the presence of 0.5 - 0.7 PM by weight of polymer organic gidroperekisi within 1 to 3 months with subsequent sensitization at pH 7 to 9 and incubated first at 37 - 56oWith over 80 to 300 rpm, and then at 2 - 8oWith within 24 - 72 hours
FIELD: polymer materials.
SUBSTANCE: invention relates to preparing aqueous cationic latexes with hollow polymeric particles serving as multifunctional additives used when preparing polymer compositions, paintwork materials, coatings (including paper coatings), and in other applications as white pigment and filler reducing density of material and internal stresses arising during formation of coatings or polymer materials. Process comprises at least three following stages: (A) preparing functional core copolymer, (B) preparing particles with core-shell morphology, and (C) ionization of functional groups of core copolymer. All process stages are carried out as a series of consecutive transformations in the same reactor without discharge of intermediate products. As monomer containing functional groups in stage A, vinylbenzyl chloride is used, and stage C represents amination of vinylbenzyl chloride units with tertiary aliphatic amines, pyridine, or derivatives of the latter.
EFFECT: improved and simplified technology and improved characteristics of cationic latex.
10 cl, 6 ex
SUBSTANCE: N (4-anilinophenyl)amide of alkenylsuccinic acid of general formula , where n=6-18 is used as a stabiliser of phenylenediamine type. The stabiliser is introduced into latex in amount 0.1-1.5 weight fractions per 100 weight fractions of rubber as aqueous alkaline liquor. While using the stabiliser of low content of ballast substance less than 3% and high content of fragments of phenylenediamine type, is suitable to be introduced into latex.
EFFECT: maintained effective stabilisation and eliminated odour nuisance.
4 tbl, 12 ex
SUBSTANCE: rubber is extracted from latex continuously by mixing latex with a coagulant. Consumption of coagulant is varied depending on the given turbidity value of serum (primary serum), which is maintained by the amount of coagulant fed. The given turbidity value of primary serum is adjusted depending on the turbidity of the serum released (secondary serum) towards the minimum consumption of coagulant to obtain minimum turbidity of the released serum.
EFFECT: method of controlling the coagulation process enables to reduce contamination of waste water through loss of partially coagulated latex with minimum consumption of coagulants.
2 dwg, 6 ex
SUBSTANCE: method of extracting butadiene-nitrile rubber from latex is carried out by feeding sodium sulphite or sodium bisulphite or sodium pyrosulphite in amount of 0.05-0.8 wt % per latex, into a stream of latex degassed beforehand, into which an antioxidant emulsion is also fed. Further, the stream of latex, containing sulphite, is directed into a coagulation apparatus into which aqueous sulphuric acid and an organic amine coagulant in form of a quaternary polymer ammonium salt are also simultaneously fed, said salt being selected from: polydimethyl diallyl ammonium chloride, a methacrylamide and dimethyl aminoethyl methacrylate hydrochloride copolymer, and polydiethyl aminoethyl methacrylate hydrochloride. The amount of the polymer ammonium salt varies from 0.2-0.5 wt %, per rubber, depending on content of leukanol content in latex in the range of 0.1-0.4 wt %, per rubber, at coagulation pH 3-6 and temperature 30-70°C.
EFFECT: invention improves environmental friendliness of the process, specifically reduces content of unreacted monomer - free acrylic acid nitrile, avoids excess synthetic coagulant and the amount of salt used for coagulation.
3 tbl, 7 ex
SUBSTANCE: nitrile rubber contains repeating units of at least one α,β-unsaturated nitrile and at least one conjugated diene and has ionic index in the range of 7-26 ppm×mol/g. The nitrile rubber is obtained by emulsion polymerisation. The obtained latex, which contains nitrile rubber, is coagulated and the coagulated nitrile rubber is washed. Polymerisation is carried out in the presence of at least one alkylthiol. Before coagulation, the pH of the latex is set to at least 6, and coagulation is then carried out in the presence of at least one salt of a monovalent metal. The latex coagulation temperature ranges from 60 to 90°C and the washing temperature ranges from 50 to 90°C. The obtained nitrile rubber is used to obtain curable mixtures which contain said rubber and at least one cross-linking agent. The curable mixtures are cured by moulding to obtain moulded articles.
EFFECT: nitrile rubber has excellent curing rate and exceptional properties of cured products.
26 cl, 7 tbl, 13 ex
SUBSTANCE: invention relates to nitrile rubber, method of its obtaining and products, obtained from it. Claimed nitrile rubber contains structure repeat units of, at least, one α,β- unsaturated nitrile and, at least, one conjugated diene, and has ion indicator in range 0-60 ppm×mole/g. Nitrile rubber is obtained by emulsion polymerisation. Obtained latex, which contains nitrile rubber, is subjected to coagulation, and then coagulated nitrile rubber is washed. Polymerisation is carried out in presence of, at least, one alkylthiolate. Before coagulation value of latex pH is set at level, at least, 6, and then is coagulated in presence of, at least, one magnesium salt. Temperature of latex before adding at least one salt of magnesium is set at value 45°C. Obtained nitrile rubber is applied for obtaining capable of vulcanisation mixtures, which contain claimed rubber, at least, one linking agent and, if necessary, additional target additives to rubbers. Capable of vulcanisation mixtures are vulcanised by casting with obtaining cast products.
EFFECT: claimed nitrile rubbers have exclusive speed of vulcanisation, as well as exclusive properties of vulcanisates.
24 cl, 13 tbl, 17 ex
SUBSTANCE: invention relates to a method of producing synthetic latex from rubber. The method comprises steps of: (a) emulsifying a binding substance containing rubber, which is dissolved in a suitable organic solvent, together with an aqueous surfactant solution to form an oil-in-water emulsion; (b) step-by-step reduction of content of solvent in the oil-in-water emulsion in two or more steps, resulting in formation of synthetic latex. Also described is a reactor with continuous mixing for removing the organic solvent from the oil-in-water emulsion, which contains rubber dissolved in the organic solvent.
EFFECT: reducing the amount of residual solvent to very low levels with high efficiency of the process.
19 cl, 2 dwg, 1 tbl, 11 ex
FIELD: process engineering.
SUBSTANCE: invention relates to latex emulsions that can be used in producing coating compositions that are not sensitive to water, have good resistance to clouding and a good ability to be sterilized in an autoclave. In some embodiments, the coating compositions are used for appltyng the coatings to such bases as cans or packaging materials for storing food and beverages. The coating compositions of the invention can be prepared by polymerizing at least one ethylenically unsaturated monomer component, a stabilizer containing a strong acid, and an initiator in a carrier to form an emulsion; by polymerizing the emulsion with at least one other ethylenically unsaturated monomer component to obtain multicomponent latex emulsion; and by the interaction of the multicomponent latex emulsion with a neutralizing agent to form a coating composition. Wherein the multicomponent latex emulsion comprises a core and a shell, the core having less functional monomers than the shell. The methods of applying the coating on bases by means of coating compositions are disclosed as well.
EFFECT: invention allows to prepare latex with the gel content of morer than 35 wt %, providing the coatings with elasticity / adhesion with good curability in a short exposure time while processing in an oven the films in on a metal base, used for container covers for beer or a beverage.
33 cl, 3 tbl, 8 ex
FIELD: microbiology and immunology, in particular immunodiagnosis.
SUBSTANCE: atypical strain of melioidose Burkholderia pseudomallei-111-6-1 with altered phenotype defected with respect to synthesis of 8 antigen and acting as immunosuppressor is used as antigen for animal immunization. Immune serum is obtained after 2 immunization cycles of animal-producer with titer in gel immunodiffusion reaction not less than 1:128.
EFFECT: immune serum with increased specific activity.
2 tbl, 2 ex