A method of producing collagen

 

(57) Abstract:

Usage: food and medical industries. The invention: the invention concerns a method of producing collagen, providing for the cleaning of raw materials, adding water, hydrolysis with a proteolytic enzyme at a constant pH and temperature, Department of collagen, its homogenization, maturation. After purification of the raw materials, it is immersed in ice water at pH 5.5 and the mixture is ground, add water in a weight ratio of 1:1, the hydrolysis is carried out at a temperature of 40-42oC and pH 11 for 1 h 45 min 3 h 30 min, and before separating the collagen pH set at the level of 5.5. 3 C.p. f-crystals.

The invention concerns a method of producing collagen or collagen films used in various fields of technology.

Collagen is a protein of 18 amino acids, which are formed collagen fibers, which is the main constituent of connective tissue in animals kept in the intestine, stomach, skin, lungs, udder and other animal organs. Previously, the collagen used mainly in its natural form in the form of treated intestines as sausage casings. Recently, collagen polonich sausage casings, surgical threads, etc.

A method of obtaining collagen/collagen film described in patent WO 81/03261 (1981), which provides for the purification of raw materials, adding water, hydrolysis with a proteolytic enzyme at a constant pH and temperature, Department of collagen, its homogenization, maturation, and in the case of obtaining the film adding a plasticizer, a crosslinking agent, and extruding. The known method, however, requires long-term hydrolysis under conditions in which it is difficult to achieve a high quality product.

The invention allows to reduce the duration of the process, and use options to reduce the impact of bacterial impact on raw materials, which is beneficial to the quality of the final product.

According to the invention, a method of producing collagen/collagen film of the type indicated above provides that after cleaning the raw materials they are immersed in ice water at pH 5.5 and the mixture is ground, add water in a weight ratio of 1:1, the hydrolysis is carried out at a temperature of 40-42oC and pH 11 for 1 h 45 min 3 h 30 min, and before separating the collagen pH set at the level of 5.5, and in the case of obtaining Plessey hydrolysis refers to the alkaline type. As alkali typically use sodium hydroxide. For the separation of a mixture of collagen, its pH should be lowered to slightly acidic level (5,5).

The separated collagen can be collected on the sieve, rinsed with water at 40oWith, as well as by centrifugation or decantation, though can be restricted to washing.

According to the invention, preferably carry out the homogenization of collagen machining at pH 3, which is achieved by adding acid and water during processing, and processing of, and adding hold until then, until the desired concentration of collagen.

In the case of obtaining collagen crosslinked form, i.e. in the form of a collagen film, the resulting crude collagen is mixed with reducing agents such as ascorbic acid and sodium sulfite in an amount up to 2 wt. and with suturing, for example, with glutaraldehyde in an amount of about 0.1 wt. and with 5-10 wt. glycerol as softener (all based on dry collagen).

The collagen obtained by the method according to the invention, can be used as a binder in meat products, as a film for packaging food and drug administration, media coverage of electronic chips, and for coating paper.

The feedstock used is preferably the internal organs of animals, in particular, parts of the gastrointestinal tract, such as first, second, and fourth stomachs (rumen, grid, abomasum, respectively), guts. Can also be used lungs, udder and large intestine of animals.

In the case of guts, their pre-process on the well-known equipment, removing content, fat and mesentery. Cleaned, washed raw material is mixed with water with ice, in which it is desirable to add acetic acid to reduce the pH to 5.5. This rapid cooling of guts and acidic environment inhibits the growth of bacteria. The raw materials thus maximally cool, not freezing it, so as freezing adversely affects the quality of collagen.

On site processing of raw materials in collagen, raw materials add clean water to the contents of the source materials in the mixture was about 50% by weight. The mixture is then heated to 40-42oC with constant stirring and add the sodium hydroxide to achieve a pH of 10.5. The temperature during the hydrolysis support level 40-42oC. it is Important that the temperature does not exceed n. When the specified temperature and pH in the mixture of proteolytic enzymes, which preferably are alcalase. At high pH and a significant number of added enzymes duration of hydrolysis can be reduced to 105-210 min (1 h 45 min 3 h 30 min). During hydrolysis alkalosis hydrolyzes proteins, which is not part of collagen, and as a result consumed alkali, so the lye is added continuously at a pH level of 10.5. The end of hydrolysis is determined by the termination of the reduction in pH, and the lye is no longer added. the pH of the mixture is reduced to 5.5 by adding acid, such as hydrochloric, acetic, citric or lactic.

At a pH of 5.5 is the compaction of collagen lumps form. This is due to the fact that the isoelectric point of this protein is about pH 5.5, which is well known. In this form of collagen is easier to separate from the mixture, for example, through a sieve or similar

Finally, the collagen is washed with water at 40oC, and then centrifuged or decanted. After centrifugation, the collagen typically contains about 22 dry matter, and after decanting for about 30 dry matter.

To use a crude collagen for certain purposes, it should nd setele type tectonically, in which along with the water, add the acid, such as hydrochloric or lactic until pH 3, and the desired concentration of collagen. After homogenization of the collagen is left to ripen at low temperature, usually at 8-10oC, within 24 hours

In the case of obtaining a collagen film, for example for use as an artificial sausage casings, collagen type crosslinking agent, e.g. glutaraldehyde in an amount of about 0.1 wt. and the softener

the glycerol in the amount of 5-10 wt. In order to get the film was bright and transparent, it is necessary to add an antioxidant, such as ascorbic acid or sodium bisulfate in an amount up to 2 wt. preferably from 0.02 to 0.1 wt.

As a by-product in the production of collagen, hydrolyzed water by evaporation and addition of vitamins, minerals and flavoring agents can be obtained feed pellets. Hydrolytic water can also be used as a liquid feed, but not long-term storage due to the fact that it spoils quickly.

As the proteolytic enzyme is preferably used alcalase 2,6 company NOVO activity of 60 units of memory in 25 ml. of This alkaligrass, this alcalase allows you to speed up the process of hydrolysis. The product obtained according to the invention has a very low fat content, which is of great importance for the quality of the collagen film.

Below the invention is illustrated by the example of its implementation.

Example 1. Purified porcine intestine in the amount of 6.5 kg immersed in ice water with a pH of 5.5. The pH-value is set by adding acetic acid. Intestine and water with ice grind, then add water from that calculation, that the total amount of water together with guts was 13 kg, i.e. about 6.5 kg of water and 6.5 kg guts.

The resulting mixture is stirred and heated to 40-41oC, adding 143 ml of 4M sodium hydroxide solution until the pH reaches of 10.5. Then the mixture was added proteolytic enzyme alcalase 2.6 L company NOVO in the amount of 150 ml the pH Value is maintained at the constant level (10,5) for 1 h 45 min by the addition of 4M sodium hydroxide solution so that the total number of added hydroxide amounted to 253 ml

After hydrolysis, the pH decrease to 5.5 by adding hydrochloric acid. The obtained collagen press and formed into large pieces.

the. Backwash time is 20-25 minutes Collagen are then harvested by centrifugation and homogenized in a vacuum mixer type tectonically. When homogenization in collagen add so much water and hydrochloric acid to obtain a pH of 3 and the collagen content of dry matter 8% of the Temperature during homogenization does not support higher 15-18oC.

After homogenization of the collagen is left to Mature for 24 hours at a temperature of 8-10oC, after which the collagen is sifted to remove lumps and impurities on the special sieve under pressure.

Example 2. The receiving film. In collagen, obtained in example 1, add ascorbic acid in the amount of 0.05% glutaraldehyde in an amount of 0.1% and glycerin in an amount of 7% Collagen then ekstragiruyut obtaining transparencies.

Expert it is clear that although the invention is described above on individual examples of its implementation, there are various modifications of the invention, without departing from its scope defined solely by the claims.

1. A method of producing collagen, including cleaning materials, adding water, hydrolysis with a proteolytic enzyme at a constant pH value and temperature, separating collagemachine pH 5.5 and the mixture is ground, add water in a mass ratio of 1 to 1, the hydrolysis is carried out at a temperature of 40 to 42oC, the pH value of not more than 11 for 105 210 min before separation of collagen establish the pH of the mixture at the level of 5.5.

2. The method according to p. 1, characterized in that the hydrolysis is carried out at a pH value of 10.5.

3. The method according to p. 1, characterized in that the separation of collagen carried out by sieving, washing with water at a temperature of 40oWith, centrifugation or decantation.

4. The method according to p. 1, characterized in that the homogenization of collagen is carried out at a pH value of 3.0 by means of mechanical processing.

 

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FIELD: film for food packing.

SUBSTANCE: claimed tubular film contains at least three polyamide layers, wherein each layer consists mixture of (mass parts) polyamide 6 70-77; polycaprolactam-hexamethylenediamine-adipic acid copolymer 5-11; amorphous resin 20-25; aromatic polyamide containing hexamethylene diamine and terephthalic acid units in molecule 3-8; and mixture of acid modified copolymers 0.3-5.

EFFECT: film of improved barrier properties, moldability, abrasive and oil resistance.

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FIELD: food-processing industry, in particular, machines for producing artificial protein casing, more particular, construction of forming head.

SUBSTANCE: head has housing with branch pipe provided with bushing. Immovable shaft with longitudinal opening for supplying of compressed air is incorporated inside housing. Immovable shaft carries sleeve arranged so as to define collagen mass slot relative to branch pipe bushing, and rotary sleeve connected with ring, on which conical roller bearings and spiral gear are disposed. Head has collagen mass supplying system connected with housing mid portion, and cooling system. Head is equipped with movable and immovable end water and mass tight gaskets respectively positioned in mid portion of housing, and water-tight gaskets arranged in its rear portion. Helical grooves of 0.8-0.9 mm depth and thread angle of 60±2 deg are formed on rotary sleeve.

EFFECT: increased efficiency and provision for producing of required amounts of products owing to complete collecting of collagen mass for forming of casing and elimination of carrying away of said mass with water.

3 cl, 4 dwg

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