The method of obtaining protein

 

(57) Abstract:

Usage: as an additive in the manufacture of bakery products for dietetic purposes, in the canning industry. The inventive method of producing a protein, comprising shredding fat-free plant material, extraction of protein electroactivated aqueous solution of NaCl at a pH of 9.2 - 9,4, the separation of the liquid phase, precipitation of protein electroactivated aqueous solution of NaCl at a pH of 4.2 to 4.7. Yield of 13 - 16%. Fractional composition of the obtained product, % of total proteins: albumin - 10,85, globulins - 46,2, glutelin - 28,1. The solubility of the protein: the amount of soluble precipitate 78,2%, the amount of insoluble precipitate with 21.4%. table 2.

The invention relates to the field of protein extraction from natural sources.

A method of obtaining protein from skim plant material extraction at 60oC aqueous solution of Ca(OH)2concentration 0,002 0,004 mol/L. the Ratio of meal to solvent of 1:10. Precipitated protein at 50oC and pH 5, created in an aqueous solution of HCl (U.S. patent N 2785155). The disadvantage of this method is the necessity of using solutions of alkali and acid, that is more close known is a method of obtaining protein from skim plant material by alkaline extraction. Extraction is carried out with an aqueous solution of NaOH at a pH slightly above the largest isoelectric point of glycerol (pH > 7).

The resulting mixture is filtered, separating the liquid phase from the precipitate, which is washed with an alkaline solution. From the liquid phase precipitated protein by acidification to pH to 4.2 with acetic, hydrochloric or phosphoric acids. Separate the protein from the liquid phase by centrifugation and dried (French patent N 2089704).

The disadvantage of this method is the necessity of using aqueous solutions of alkali and acid, which complicates the technological process, as well as carrying out the extraction at a pH of not allowing to achieve maximum yield of protein from the material.

The aim of the invention is the reduction of process operations training extractant and precipitating solution, increasing the yield of the protein and improve its quality.

The invention consists in the extraction protein extraction from crushed fat-free plant material. The liquid phase containing the protein is separated from the material. The protein from the liquid phase precipitated and then separated from the liquid phase and dried.

Extraction of protein carry out the extraction of electroic is electroplated in an aqueous solution of NaCl at a pH of 4.2 to 4.7.

The extraction was carried out for 15 minutes as extractant using of electroactivated aqueous solution of NaCl concentration 1 g/l m pH 12, which for the extraction of protein from the neutral material is neutralized, resulting in the extraction is carried out at a pH of 9.2 to 9.4. Separated from the material liquid phase having a pH > 7, during the precipitation of the protein electroactivated aqueous solution of NaCl concentration 1 g/l with a pH of 2.3 neutralized them and deposition occurs in the interval pH of 4.2 to 4.7.

Electroactivated aqueous solution consider the solution subjected to electrolysis in a cell with the separation of cathode and anode space aperture. In the electrolysis of water with an inert anode, excluding the transfer of substances from the electrode into the solution, the electrode is oxidation and reduction of water molecules: in the area of the cathode 2H2O+H 2O2+2OH-(hydrogen system, catholyte), in the area of the anode (oxygen system, the anolyte).

In near-electrode zones are formed of different redox systems, the concentration of electrically charged ions (pH, redox potential), which varies considerably.

If the water is exposed electry causes a low concentration in catholyte hydrogen ions, i.e. high pH value.

On the surface of the anode along with the oxidation of water in chloride solutions is the separation of gaseous chlorine:

2Cl - 2e _ Cl2< / BR>
Chlorine is partially soluble in water with formation of chlorapatite and hydrochloric acid:

< / BR>
This may explain the strong decolorizing ability of electroactivated aqueous solutions of NaCl.

Depending on the conditions on the surface of the anode also reactions proceed with the formation of HClO2, ClO-2, ClO-3, ClO-4ClO2. In the anolyte of this type (chloride) formed active free radicals ClO'; Cl'; OH'.

During the process, the formation of molecular oxygen by recombination of two atoms or ClO radical bimolecular reaction of the second order:

2ClO _ Cl2+ O2< / BR>
The reaction of oxidizing or reducing ability of the products of the anodic or cathodic reactions increases with changes in the concentration of hydrogen ions, respectively, with the decrease in the pH of the anolyte or increase the pH of Catolica.

Electroactivated liquid aqueous medium has the following anomalous physical and chemical properties:

advanced explore the surface of solid particles, in electroactivated liquid medium;

has catalytic ability;

allows you to neutralize the corrosive properties of the liquid systems;

enhances the properties of the substances dissolved in the electroactivated liquid medium;

has a high extraction capacity;

has biological activity, including antibacterial properties and properties of stimulant metabolic processes.

The fraction of activated solution from the anode (the anolyte) and the area of the cathode (catholyte) have different properties. So, in the anolyte there is a decrease in pH to sharply acidic region (1.5 to 1.0 and below) and the increase in redox potential; Catolica an increase in pH (up to 10-13) to extremely alkaline region and a decrease in redox potential.

When removing the protein by the method of use for the extraction and deposition of electroactivated aqueous solutions eliminates the need to use to obtain food chemicals. Electroactivated aqueous solution is obtained from an aqueous solution of sodium chloride, which is a food product. Extractively water resposablity, use it as extractant allows to obtain protein with less severe colouring, because of the bleaching ability of the electroactivated aqueous solutions. To obtain solutions of alkaline and acidic characters by electric activation requires fewer process operations than when receiving fluids of the same properties of the chemicals.

The sequence of technological operations in obtaining the extractive agent and precipitating solution:

1) from the chemical reagents

< / BR>
2) by electric activation

< / BR>
As can be seen, the use of chemical reagents increases the number of process operations training extractant and the precipitating solution. When electric activation are both essential fluids get one technological operation.

The quality of the proposed method the protein is not getting worse, its color is more bright. The yield of protein is increased compared with the method of its extraction with the use of chemical reagents.

The choice of the interval of pH values at which the extraction is carried out, caused the highest yield of protein compared with fewer pH, henia on the yield of protein is not affected, but at pH > 4,7 time is increased deposition of protein.

These studies are listed in table 1.

Example. The experiment was conducted in laboratory conditions. Electroactivated aqueous solution of NaCl was obtained in a laboratory setup electroactuator of aqueous NaCl concentration of 1 g/L. protein Extraction was carried out with 10 g of crushed sunflower meal electroactivated aqueous solution of NaCl with a pH of 12 for 15 min at pH extraction of 9.4; temperature logging process 20oC. the Process is carried out with constant stirring in a beaker. The solid phase is separated from the liquid by filtration through a folded paper filter. Precipitation of protein from the liquid phase carried out by acidification of electroactivated aqueous solution of NaCl with a pH of 2.3 in the beaker at t 20oC for 30 min, the pH of the deposition of 4.5. Precipitated protein is separated from the liquid phase by filtration through a folded paper filter at t 20oC. Drying the protein is held to a constant weight in a drying Cabinet at t 60oC.

The rest of the experiments, with values:

the pH of the extraction 9,0; 9,2; 9,3; 9,6;

the pH of the deposition 4,2; 4,5; 4,5; 4,7

carried out similarly and the results of these experte), which is typical skim material, so the extraction of protein from meal other oilseed crops will be obtained similar results.

For comparison in the table.2 shows the values for some indicators seeds and sunflower meal and soybean.

In a production environment electroactivated aqueous solutions can be obtained at industrial installations for electric activation of water:

UAV-7 performance up to 2000 l/h when the pH of the resulting solution of 1.5 13;

"Progress" capacity 2000 l/h when the pH of the resulting solutions 3 12.

Fractional composition of the produced protein from the total protein albumin 10,85, globulins 46,2, glutelin 21,1. The solubility of the obtained protein: the amount of soluble precipitate 78.2% of the amount of insoluble sludge 21,4%

The protein obtained in the described manner, can be obtained as an additive in the manufacture of bakery products for dietetic purposes, in tin production in the manufacture of vegetable concentrates.

A method of producing a protein, comprising grinding skim material, removing the protein from the extraction, separation of the liquid phase containing the protein, sedimentation FR 9,4, the deposition carried out electroactivated aqueous solution of NaCl at pH 4.2 to 4.6.

 

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FIELD: food-processing industry, in particular, processing of white mustard seeds free from anti-nutritive substance of sinalbin.

SUBSTANCE: method involves processing ground white mustard seeds by extracting sinalbin thioglycoside at water temperature of at least 95-1000C for at least 40 min; separating phases upon completion of extraction procedure; repeatedly washing solid phase at the same temperature and drying by known methods until residual moisture content is not in the excess of 10%. Resultant product may be used as food additive.

EFFECT: improved quality of product free from anti-nutritive substances of sinalbin and decomposition products thereof.

1 tbl, 1 ex

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