The method of obtaining material for drainage

 

(57) Abstract:

The proposed method can be used in ophthalmology in surgical treatment of patients with glaucoma. The method consists in obtaining material for drainage, connective tissue use the pericardium, which secrete collagen, then treated with a solution of collagen glycosaminoglycans in concentration from 50 to 400 μg/g, and then dried at 4oC for 16-24 h before skinning.

The invention relates to medicine and, more specifically, to ophthalmology, and can be used in surgical treatment of patients with glaucoma.

A method of obtaining material from the tissue of the pericardium [1] which is used in the treatment of progressive myopia and selected by the authors for the prototype because it is the closest technical solution.

The task of the invention to provide material for drainage with high enzymatic stability, low solubility and high filtration capacity.

The technical result that is achievable with the use of the invention is to obtain antiglaucomatous drainage, capable of a long time to filter Bolshaia is achieved by the drainage for antiglaucomatous operations consists of collagen, pericardium farm animals, which processed the sulfated glycosaminoglycans (sgag) at a concentration of from 50 to 400 mcg/g, dried at 4oC for 16-24 h before formation of the film, then the film again handle of sgag from 200 to 600 µg/g, dry and crumble in the form of a roll.

It is known that collagen pericardium consists mainly of collagens I and III types, which unlike collagen pig sclera has a low solubility and greater resistance to the action of proteolytic enzymes. Additional strength and stability of the collagen gets in the processing of sgag, as in this case, the structuring of collagen fibers.

sgag/keratan-sulfate, chondroitin-sulfate, heparan-sulfate, heparin/ are part of the connective tissue and regulate it water-salt metabolism, holding large amounts of water. When applying them to the surface of the collagen films have strong dehydrating properties and therefore amplify the filtered water from the anterior chamber of the eye. When rolling in the rolls perform the inner surface drainage and prevent its overgrowth. the ElCat and processed within 24 h in 0.1 HOH, then it is put in a 1-2 M solution of acetic acid for 24 h, dialist against 0.1 M acetic acid buffer solution of sodium.

Poured into a solution of collagen sgag concentration from 50 to 400 mcg/g At a concentration of sgag below 50 µg/g linking of collagen fibers does not occur, and if sgag concentration above 40 µg/g stitching stops.

After that, the solution of collagen is poured into moulds and dried at 4oC for 16-24 hours At the drying less than 16 h collagen, skateway in the roll stick together, and when dried 24 h in the films formed microcracks. Then the film again treated sgag concentration from 200 to 600 µg/g, dry and crumble in the form of a roll. When the sgag concentration below 200 µg/g surface drainage does not have sufficient enzymatic stability, and at concentrations above 600 µg/g factor binding sgag surface is reduced, and the excess of sgag quickly washed away from the surface drainage, in addition, the inner surface drainage with a large concentration becomes more viscous, which may inhibit fluid filtration.

The dry film is rolled into a roll, then cut with a razor for a certain size.

Example 1. To the resulting dia is ivali at 4oC for 16 h before formation of the film, and then processed sgag concentration of 200 ág/g, was dried in a constant current of air and rolled into a roll. After the final drying drainage sterilized by irradiation dose of 2.5 Mgrad.

Example 2. Received and otdelitelnogo the pericardium collagen (10 g) was added with constant stirring sgag concentration of 250 μg/g, was poured into molds and dried at 4oC for 18 h before formation of the film, after which the film was re-processed sgag concentration of 400 mcg/g, was dried in a constant current of air and rolled into a roll, and then finally dried and the resulting drainage was sterilizable g-irradiation dose of 2.5 Mguard.

Example 3. To dialysis collagen pericardium (100 g) under stirring was added 400 µg/g sgag and poured into the mold, dried at 4oC for 24 h before formation of the film, after which the film was processed sgag concentration of 600 µg/g, was dried her and rolled into a roll, finally dried and the resulting drainage sterilized by g-irradiation.

The method of obtaining material for drainage, including the processing of the pericardium, characterized in that is used as raw material of ncentratio from 50 to 400 mcg/g then dried at 4oC for 16 - 24 h before formation of the film, after which the film is treated with glycosaminoglycans from 200 to 600 µg/g, dry and crumble in the form of a roll.

 

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