The way to increase radioprotective action of vitamin e
(57) Abstract:The invention relates to radiobiology and solves the problem of increasing radioprotective action of vitamin E and increasing the life span of irradiated animals. This result is achieved in that in the method, including intravenous animal 3 days before irradiation - rays at a dose of 800 glad vitamin E, vitamin E is administered in combination with hialuronowy acid, while doses of vitamin E and hialuronowy acid respectively and 80-100 10-20 mg/kg of animal weight. table 1. The invention relates to radiobiology, and in particular to methods of increasing the radioprotective action of vitamin E.There are many radioprotectors used to prevent the death of animals subjected to irradiation by ionizing radiation. Among the most effective are mercaptoethylamine, serotonin and histamine, Maximin, some vitamins. However, the known radioprotectors or maintain the efficiency of the body's defence or demand for its manifestation is the use of large doses that leads to toxic effects.Special attention has been paid recently to the study of radioprotective on the new chain.< / BR>in and t a m I n E (tocopherol)
According to modern concepts vitamin E prevents the destructive action of molecular oxygen, is capable of nonenzymatic way to attack the double bonds of polyunsaturated fatty acids included in the composition of tissue lipids, i.e., to show antioxidant activity.Known technical solutions aimed at improving the antioxidant activity of vitamin E through the use of its combinations with substances of different chemical nature.Closest to the proposed adopted for the prototype is a way to increase radioprotective action of vitamin E, according to which animal 3 days before x-ray irradiation at a dose of 800 glad injected a mixture of vitamin E (200 mg/kg) and enol forms of the lactone diketogulonic acid (285 mg/kg).The disadvantage of this method is that the used mixture only slightly (13-15%) enhances the radioprotective effect of vitamin E and do little to protect animals from the effects of ionizing radiation.The invention solves the problem of increasing radioprotective action of vitamin E and increasing the life span of irradiated animals.The advantages of the proposed method over the known are that it provides a lower dose of vitamin E in 2-2,5 times and provides a more pronounced radioprotective effect due to joint injection with hyaluronic acid, which is reflected in the significant increase in life span of irradiated animals (median survival 25 days compared to 13 days by a known method).It is important to emphasize that the introduction of smaller doses of vitamin E (less than 80 mg/kg) and hyaluronic acid (less than 10 mg/kg) does not provide radioprotective effect of this combination, and the large doses of vitamin E (100 mg/kg) and hyaluronic acid (20 mg/kg) does not lead to additional strengthen the radioprotective effect and is therefore impractical.The basis of the synergism of the claimed combination is not a simple additive effect, and different mechanism of radioprotective action, as shown by the example of other chemical compounds.It should also be noted that hyaluronic acid E.Example 1. 40 outbred white mice weighing 251,5 g were divided into 4 equal groups by random selection. All animals were irradiated with g-rays in the apparatus ROOM-3 in aluminum chambers to achieve a dose of 800 rad. 3 days before irradiation to mice 1 (control) group were injected intravenously with 1 ml of saline, the mice of group II-1 ml of an aqueous solution containing 0.25 mg of hyaluronic acid (used domestic product of hyaluronic acid obtained from the crests of birds on THE 9185-003-17204830-93.) (10 mg/kg), mice of group III and 1 ml of the oil solution containing 2 mg of vitamin E (used Pharmacopeia drug of vitamin E in the form of a solution of tocopherol acetate in oil.) (80 mg/kg) and mice of group IV 1 ml of water-oil emulsion containing 0.25 mg of hyaluronic acid and 2 mg of vitamin E (respectively 10 and 80 kg/kg). The animals were observed for 30 days. Monitoring found that all animals 1 (control) group died from radiation sickness within 5-8 days (cf. 6, 5 days). The life span of irradiated mice of group II and III were respectively 7, 8 and 11, 5 days, and mice of group IV 23.2 day, i.e., respectively, above of 2.97 and 2.01 times than when using separate connections.Example 3. 40 outbred white mice weighing 251,5 g were divided into 4 equal groups and further went on to example 1 except that the animals of group IV was injected intravenously at 1 mg emulsion containing 0.5 mg (20 mg/kg) of hyaluronic acid and 2.5 mg (100 mg/kg) of vitamin E. the life span of irradiated animals of the 1st group 6.43 day, mice groups II and III respectively of 7.64 and 11,54 days, and group IV 27.4 days, i.e. increased 3.58 and 2.37 times compared to using separate connections.Technical-economic indicators of well-known and proposed methods are presented in the table.Thus, the invention allows in 2-2,5 times lower dose of vitamin E and about 2 times to increase the life span of irradiated animals compared with the known method. The method of increasing the dose of 800 glad vitamin E, characterized in that the vitamin E is administered in combination with hyaluronic acid, with doses of vitamin E and hyaluronic acid are respectively 80 100 10 20 mg/kg of animal weight.
SUBSTANCE: before applying substitute hormonal therapy (SHT) on should evaluate antithrombogenic activity of vascular wall in women. For this purpose one should determine quantitative values of ADP-induced aggregation of thrombocytes, activity of antithrombin III in blood and fibrinolytic blood activity both before and after "cuff"-test. Then one should detect the indices calculated as the ratio of mentioned values both before and after carrying out the mentioned test. If mentioned indices are decreased against the norm by 20-40% women should be prescribed to undergo SHT at additional introduction of aspirin and supradin. The method provides prophylaxis of cardio-vascular diseases in this category of female patients due to correcting affected functional activity of vascular endothelium.
EFFECT: higher efficiency of prophylaxis.
1 cl, 1 ex, 4 tbl
FIELD: veterinary science.
SUBSTANCE: about 20-25 d before calving one should introduce intramuscularly 0.5%-sodium selenite solution for cows at the dosage of 10 ml. Twice before and twice after calving at 10-d-long interval - tetravit at the dosage of 10 ml at the content of 50000 IU vitamin A, 25000 IU vitamin D, 20 mg vitamin E and 5 mg vitamin F per 1 ml. Succinic acid should be introduced 20-25 d both before and after calving at the dosage of 1.0 g. The method provides efficient correction of the main values of homeostasis in cows after calving.
EFFECT: higher efficiency of normalization.
2 ex, 4 tbl
FIELD: medicine, radiation therapy.
SUBSTANCE: it is suggested to apply mixture prepared ex tempore out of: 5 mg hyoxison ointment, 1 ml 10% alpha-tocopherol acetate and 100 mcg superlymph-1 dissolved in 1 ml sterile distilled water. The suggested mixture should be applied twice daily onto affected part with thin layer to accelerate regeneration of affected tissue.
EFFECT: higher efficiency of therapy.
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to an antiviral medicinal preparation. The preparation comprises ribavirin, phosphatidylcholine, cholesterol, α-tocopherol, sucrose, sodium chloride and represents lyophylizate in isotonic solution. Invention provides preparing the liposomal ribavirin eliciting high biological and therapeutic effectiveness with low toxicity.
EFFECT: improved, enhanced and valuable medicinal properties of preparation.
3 cl, 2 tbl
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to manufacturing medicinal preparations with wound-healing effect, in particular, to preparation with wound-healing effect comprising an agent stimulating epithelization. The wound-healing agent comprises vitamin E in concentrations 0.01-5.0 wt.-% in chitosan gel. Preferable variant represents applying chitosan gel formed by fractionated low-molecular chitosan of molecular mass from 10 to 20 kDa, or from 20 to 50 kDa, or from 50 to 300 kDa taken in the concentration from 1.0 to 10.0 wt.-%. Except for, it is possible additional applying antibacterial agent in wound-healing agent taken among the following order: chloramfenicol, lyncomycin, metronidazol, ciprofloxacin, benzalkonium chloride, additional using an antibacterial agent and lidocaine in agent, additional using an antibacterial agent and adrenaline in agent, and additional using hydrocortisone in the concentration 0.5 wt.-% in agent. Invention provides preparing wound-healing effect that occurs early essentially in epithelization and without formation of colloidal scars and reduces time for appearance of tissue regeneration markers.
EFFECT: valuable properties of agent.
8 cl, 2 tbl, 3 ex