The way to increase radioprotective action of vitamin e

 

(57) Abstract:

The invention relates to radiobiology and solves the problem of increasing radioprotective action of vitamin E and increasing the life span of irradiated animals. This result is achieved in that in the method, including intravenous animal 3 days before irradiation - rays at a dose of 800 glad vitamin E, vitamin E is administered in combination with hialuronowy acid, while doses of vitamin E and hialuronowy acid respectively and 80-100 10-20 mg/kg of animal weight. table 1.

The invention relates to radiobiology, and in particular to methods of increasing the radioprotective action of vitamin E.

There are many radioprotectors used to prevent the death of animals subjected to irradiation by ionizing radiation. Among the most effective are mercaptoethylamine, serotonin and histamine, Maximin, some vitamins. However, the known radioprotectors or maintain the efficiency of the body's defence or demand for its manifestation is the use of large doses that leads to toxic effects.

Special attention has been paid recently to the study of radioprotective on the new chain.

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in and t a m I n E (tocopherol)

According to modern concepts vitamin E prevents the destructive action of molecular oxygen, is capable of nonenzymatic way to attack the double bonds of polyunsaturated fatty acids included in the composition of tissue lipids, i.e., to show antioxidant activity.

Known technical solutions aimed at improving the antioxidant activity of vitamin E through the use of its combinations with substances of different chemical nature.

Closest to the proposed adopted for the prototype is a way to increase radioprotective action of vitamin E, according to which animal 3 days before x-ray irradiation at a dose of 800 glad injected a mixture of vitamin E (200 mg/kg) and enol forms of the lactone diketogulonic acid (285 mg/kg).

The disadvantage of this method is that the used mixture only slightly (13-15%) enhances the radioprotective effect of vitamin E and do little to protect animals from the effects of ionizing radiation.

The invention solves the problem of increasing radioprotective action of vitamin E and increasing the life span of irradiated animals.

The advantages of the proposed method over the known are that it provides a lower dose of vitamin E in 2-2,5 times and provides a more pronounced radioprotective effect due to joint injection with hyaluronic acid, which is reflected in the significant increase in life span of irradiated animals (median survival 25 days compared to 13 days by a known method).

It is important to emphasize that the introduction of smaller doses of vitamin E (less than 80 mg/kg) and hyaluronic acid (less than 10 mg/kg) does not provide radioprotective effect of this combination, and the large doses of vitamin E (100 mg/kg) and hyaluronic acid (20 mg/kg) does not lead to additional strengthen the radioprotective effect and is therefore impractical.

The basis of the synergism of the claimed combination is not a simple additive effect, and different mechanism of radioprotective action, as shown by the example of other chemical compounds.

It should also be noted that hyaluronic acid E.

Example 1. 40 outbred white mice weighing 251,5 g were divided into 4 equal groups by random selection. All animals were irradiated with g-rays in the apparatus ROOM-3 in aluminum chambers to achieve a dose of 800 rad. 3 days before irradiation to mice 1 (control) group were injected intravenously with 1 ml of saline, the mice of group II-1 ml of an aqueous solution containing 0.25 mg of hyaluronic acid (used domestic product of hyaluronic acid obtained from the crests of birds on THE 9185-003-17204830-93.) (10 mg/kg), mice of group III and 1 ml of the oil solution containing 2 mg of vitamin E (used Pharmacopeia drug of vitamin E in the form of a solution of tocopherol acetate in oil.) (80 mg/kg) and mice of group IV 1 ml of water-oil emulsion containing 0.25 mg of hyaluronic acid and 2 mg of vitamin E (respectively 10 and 80 kg/kg). The animals were observed for 30 days. Monitoring found that all animals 1 (control) group died from radiation sickness within 5-8 days (cf. 6, 5 days). The life span of irradiated mice of group II and III were respectively 7, 8 and 11, 5 days, and mice of group IV 23.2 day, i.e., respectively, above of 2.97 and 2.01 times than when using separate connections.

Example 3. 40 outbred white mice weighing 251,5 g were divided into 4 equal groups and further went on to example 1 except that the animals of group IV was injected intravenously at 1 mg emulsion containing 0.5 mg (20 mg/kg) of hyaluronic acid and 2.5 mg (100 mg/kg) of vitamin E. the life span of irradiated animals of the 1st group 6.43 day, mice groups II and III respectively of 7.64 and 11,54 days, and group IV 27.4 days, i.e. increased 3.58 and 2.37 times compared to using separate connections.

Technical-economic indicators of well-known and proposed methods are presented in the table.

Thus, the invention allows in 2-2,5 times lower dose of vitamin E and about 2 times to increase the life span of irradiated animals compared with the known method.

The method of increasing the dose of 800 glad vitamin E, characterized in that the vitamin E is administered in combination with hyaluronic acid, with doses of vitamin E and hyaluronic acid are respectively 80 100 10 20 mg/kg of animal weight.