The way to get oxytetracycline
(57) Abstract:Usage: medical industry, production of antibiotics. The essence of the invention: upon receipt of the antibiotic oxytetracycline produce cultivation of Streptomyces rimosus in a nutrient medium containing sources of nitrogen, carbon, mineral salts under conditions of aeration, mixing, regulation of the content of ammonia nitrogen and pH, while in the process of cultivation carried out dolive carbonaceous substrate in an amount sufficient to maintain the viscosity of the culture liquid at 60 100.10-3PA.with, and the first topping is produced when reaching the viscosity of the culture fluid 60.10-3PA.with. The invention relates to the medical industry, namely the method of obtaining biologically active compounds, in particular of the antibiotic oxytetracycline.A method of obtaining oxytetracycline  by cultivation of a producer in a nutrient medium containing sources of carbon, nitrogen, under conditions of aeration and mixing. However, due to increasing the viscosity of the culture liquid in the fermentation process with 10.10-3PA.to 200.10-3PA.is the deterioration of the MIA is to increase the level of antibioticaccutane.The aim is achieved in that in the method of producing the antibiotic oxytetracycline by growing producer Str.rimosus in a nutrient medium containing sources of carbon, nitrogen, mineral salts, in the conditions of aeration and mixing is additionally adjusting the viscosity of the feed solution of the substrate in the fermentation process.Example 1.Vegetative seeds producer of the antibiotic oxytetracycline grown on a medium containing, by weight.corn flour 7,0
corn extract 2,4
fish oil 0,5
soy flour 0,5
ammonium sulphate 0,6
in volume 8 of the 10% of the inoculated fermentation medium of the following composition, wt.corn flour 14
ammonium sulphate 0,72
propanol B-400 0,1
soy flour 0,72
chloride cobalt 0,0003
The fermentation is carried out in the apparatus Ankum 2 M with a capacity of 10 L. the Initial amount together with seeds of 6.6 liters of the fermentation Process is carried out at 27 1oC, aeration 1 volume of air to 1 volume of the medium, the speed of rotation of the stirrer 600 rpmIn the course of the process control and regulate the content of ammonium nitrogen, carbohydrates, pH, and viscosity.The viscosity of ODA is and begin feeding glucose with such speed, to viscosity values were in the range of 60 100.10-3PA.with.When the concentration of ammonia nitrogen in the culture fluid will be reduced to 0.2 to 0.4 mg/ml, begin to apply a 25% solution of ammonium sulfate, maintaining the concentration of ammonium nitrogen in the culture fluid of 0.2 to 0.4 mg/ml While lowering the pH to 5.8 stop filing solution of ammonium sulfate and begin to regulate the pH at 5.8 to 6.0 submission of a 10% ammonia solution.When increasing the volume of the culture fluid in the apparatus to 8 l begin daily cast of 250 to 300 ml of the culture fluid. The activity of oxytetracycline, the volume of the culture fluid is drained from the apparatus 300 h of growth was 26000 U/ml, 8 l, respectively.The drained volume of the culture fluid during the process 2.5 l with an average activity 16400 IU/mlExample 2.Vegetative AMF inoculum producer of the antibiotic oxytetracycline grown in flasks, inoculated seed medium in the apparatus with a capacity of 750 litres, containing, by weight.corn flour 7,0
corn extract 2,4
soy flour 0,5
ammonium sulfate 0,6
fish oil 0,5
and grow seeds for 24 to 30 h perelom in the amount of 200 l inoculated fermentation medium of the following composition, wt.corn flour 14
corn extract 3,0
soy flour 0,72
ammonium sulfate 0,72
propanol B-400 0,1
chloride cobalt 0,72
The process of cultivation are in fermenters with a capacity of 3 m3when 27 1oC, overpressure -0,04 MPa, aeration 1 volume of air to 1 volume of medium per minute, the speed of the mixer 170 rpmBy reducing the content of ammonium nitrogen to 0.4 mg/ml begin filing of a 25% aqueous solution of ammonium sulfate, maintaining the concentration of ammonium nitrogen in the culture fluid of 0.2 to 0.4 mg/ml While lowering the pH to 5.8 stop filing solution of ammonium sulfate and begin to regulate the pH at 5.8 to 6.0 submission to the office of 25% ammonia solution. In the fermentation process control and regulate the content of ammonia nitrogen, pH, and viscosity.When you reach a viscosity 60.10-3PA.with 40 to 50 h produced 1 topping 200 l (10% of the volume of the medium) of the hydrolysate of corn flour, the second and third dolive carry on the viscosity of the culture fluid, keeping it in the range of 60 100.10-3PA.with. Foam breaking exercise as in the known method: up to 100 hours as foaming produce a supply of fish oil.is etracycline 206 h in culture liquid, performance was 18530 U/ml of 2.8 m3; 52 billion Units. 0,084 billion U/m3h, respectively.By a known method activity values, volume, removal of the antibiotic in the culture fluid, performance 203 h growth are 19330 U/ml; 2.2 m3; 42 billion Units. 0,07 billion Units./m3hExample 3.The fermentation is carried out as in example 2, but in the fermentation process for foam breaking is used only propanol B-400. The use of fat as a defoamer and a carbon-containing substrate is eliminated.The activity value of the culture fluid volume, removal of the antibiotic, performance 156 h of growth was 14000 Ed./ml 2.8 m3; eat 39,1 billion Units. performance 0,084 billion U/m3including Indicators of the biosynthesis of the prototype presented in example 2. The way to get oxytetracycline by cultivation of Streptomyces rimosus in a nutrient medium containing sources of nitrogen, carbon, mineral salts under conditions of aeration, mixing, regulation of the content of ammonia nitrogen and pH, followed by separation of the target product, characterized in that in the process of cultivation carried out dolive carbon-containing substrate in the first topping is produced when reaching the viscosity of the culture fluid 60 10-3A pass.
FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.
EFFECT: improved preparing method.
15 cl, 4 ex
FIELD: canned food industry.
SUBSTANCE: method involves preparing fruits; adding sugar solution; providing cavitational grinding of fruits in sugar solution and separating pulp under aseptic conditions; providing cavitational grinding by dispersing under atmospheric pressure in mixture of fruits and sugar solution of mycelium produced after extracting of Mortierella zychae micromycet biomass with non-polar liquefied gas; sequentially extracting biomass with water, alkaline, water, acid, water, alkaline, and water; introducing resultant solid residue into sugar solution in an amount of about 0.06% by weight of fruits.
EFFECT: reduced consumption of power for producing of canned food, improved quality of canned food owing to improved retention of color of basic material, and increased resistance to breaking of canned food during storage.
FIELD: food industry, confectionary industry.
SUBSTANCE: for the purpose to obtain jelly marmalade with a wide spectrum of vitamin activity and unconventional pleasant combination of organoleptic properties one should prepare the syrup by applying sugar, pectin and an aqueous extract of the mixture of baikhovi black tea, baikhovi green tea, Centaurea flowers, calendula flowers, mountain cranberry leaves, hibiscus flowers and dog rose fruit. While preparing it is necessary to supplement the syrup with citric acid, sodium lactate, "Mountain cranberry" and "Cream" aromatizing agents and a preparation obtained out of Mortierella exigua micromycete biomass by the preset technique to be further spread, formed, dried and packed as ready-to-use products.
EFFECT: higher efficiency.
SUBSTANCE: invention relates to technology of glycopeptides purification. Clarified liquid containing eremomycin is compacted with sulphuretted stirol-divinylbenzene cationite, elution is carried out and neutralised eluate is applied on the column with hydrophobic stirol divinylbenzene sorbent. After washing column with water, eremomycin is displaced with solution of isopropyl alcohol with addition of acetic acid and crystallisation of target product is carried out.
EFFECT: obtaining a pure product with a high output at a high process productivity.
6 cl, 3 ex
FIELD: microbiology; medicine.
SUBSTANCE: strain Amycolatopsis orientalis subsp.eremomycini All-Russian collection of industrial microorganisms Ac-807 is eremomycin producent and can be used as source of eremomycin in medicine for treatment of severe infections.
EFFECT: increase of eremomycin output.
2 tbl, 1 ex
SUBSTANCE: proposed strain of Nonomuraea roseoviolacea subsp carminata (Actinomadura carminata) BKIIM-S1081 is obtained as a result of multiple stage mutagenic effect of nitrimethylbiurets, methylnitrosoguanidine and gamma rays 60Co on the initial strain № All Russian collection of industrial microorganisms S-806 with subsequent extraction of mutants on media, containing streptomycin and daunorubicin as selective agents, and deposited under the number All Russian collection of industrial microorganisms S-1081. Carminomycine is obtained from biosynthesis of a strain producer, treatment of culture liquid with acetone and oxalic acid, alkalisation of the solution, extraction of antibiotic using chloroform, re-extraction of antibiotic using an aqueous buffer at pH=5.0, repeated extraction using organic solvents at pH=8.0, concentration of the extract, filtration through a neutral PTFE sorbent and extraction of antibiotic through precipitation from the solution in dichloromethane-hexane or petroleum-ether in form of a base.
EFFECT: increased output of carminomycine antibiotic, efficient in therapy of soft tissue sarcoma, lymphosarcoma, reticulosarcoma, chorionepithelioma, acute myeloblastoma and lymphoblastoma, chronic myeloid leukaemia (120 mcg in 1 ml of culture liquid).
2 cl, 1 tbl, 1 ex
FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and can be used in medical industry during production of antibacterial antibiotic lincomycin hydrochloride which is efficient in treating infections caused by gram-positive agents, including methicillin with stable and certain anaerobic bacteria. The method involves culturing a Streptomyces roseolus VKPM S-1082 strain, which is a lincomycin producer, on a culture medium with given composition, extraction of lincomycin with organic solvents from the native solution, extraction of lincomycin with subsequent isolation. The obtained extract is concentrated and crystallised to obtain lincomycin in hydrochloride form.
EFFECT: invention increases output of lincomycin.
2 cl, 1 tbl, 2 ex
SUBSTANCE: method involves cultivation of strain Streptoalloteichus cremeus subsp. tobramycini VKPM S-1084, producer of apramicyne. Emanation of apramicyne from culture fluid with sorption on carboxylic cationites followed by chromatographic purification with two-column method by stepwise elution with solutions (0.19-0.22)n of ammonia. The resulting eluate is concentrated in vacuum and precipitated with excess of acetone apramicyne in the form of sulfate.
EFFECT: increased output of apramicyne.
2 cl, 1 tbl, 2 ex
SUBSTANCE: Amycolatopsis fructiferi subsp. ristomycini 8765 strain comes from mutant transformation of Proactinomyces fructiferi var. ristomycini 5339 strain properties as a result of consequent three-step actions on the stock strain by methylnitrosoguanidine, ultraviolet and gamma-radiation of 60Co with following selection of mutated strain possessing increased antibiotic activity. Obtained strain differs from already known one with growth form on diagnostic agar media, carbon sources assimilation rate (saccharose, starch, rhamnose, and mannose) chromogenic ability and antibiotic activity which is 5 times higher of Proactinomyces fructiferi var. ristomycini 5339 strain activity and is equal to 2000 mcg/ml. Strain is deposited to All Russian Collection of Industrial Microorganisms, collection # S1085.
EFFECT: invention can be used in medical industry for antibacterial antibiotic ristomycin manufacture; ristomycin is effective in treatment of infections induced by gram-positive microorganisms.
SUBSTANCE: compound WS727713 is prepared by cultivation in a culture medium of Pseudonocardia FERM BP-7570 ray fungus strain producing such compound, and recovered from a culture fluid. The compound WS727713 is used as a melanocortin receptor modulator and applied for melanocortin receptor modulation while preparing a drug or a beauty preparation and preparing a pharmaceutical composition. Also, there are presented a pharmaceutical composition containing the compound WS727713, a method involving the introduction of specified compound in a human or animal body, and its application for treatment or prevention of the diseases responsive to melanocortin receptor regulation.
EFFECT: group of inventions exhibits the activity of a melanocortin receptor modulator.
12 cl, 3 dwg, 4 tbl, 1 ex