The method of producing the antibiotic kanamycin
(57) Abstract:Usage: medical industry. The inventive method of producing the antibiotic kanamycin carry out the cultivation producer Streptomyces kanamyceticus in a nutrient medium containing sources of carbon, nitrogen, phosphorus and mineral salts under conditions of aeration and mixing, while in the process of cultivation spend dolive hydrolyzed corn flour or sterile water in a quantity sufficient to maintain the viscosity at 60 100103Pas, and the first topping is produced when reaching viscosity 40103A pass. The invention relates to the medical industry, namely the method of production of biological compounds, in particular of the antibiotic kanamycin.The known method  obtain the antibiotic kanamycin by cultivation of a producer in a nutrient medium containing sources of carbon, nitrogen, phosphorus, mineral salts under conditions of aeration and mixing. Upon reaching the concentration of kanamycin in the culture fluid of 1500 µg/ml produce adding sterile water to a volume of 3 to 4 m3for 8 to 12 hours to drain.During cultivation producer Str. kanamyceticus viscosity tion affects the mass and heat transfer between the cell and the environment. This ultimately leads to lower levels of antibioticaccutane biologically active kanamycin and increase the content in the culture fluid biologically inactive kanamycin Century To the end of fermentation to 100 hours, the concentration of kanamycin in the culture fluid is 3100 µg/ml Volume of culture fluid is 37,3 m3. Eat kanamycin in the culture fluid is 115,6 kg Solution to this problem is the improvement of mass transfer is proposed in the present method.The aim of the invention is to increase the level of antibioticaccutane kanamycin And by reducing the content of kanamycin In the culture fluid.The objective is achieved by the fact that in the claimed method of producing antibiotic by growing producer Str. kanamyceticus in a nutrient medium containing, by weight. corn flour 8,0 12,0; soy flour 2,0 2,5; aluminum sulfate 0,009 0,011; iron sulfate 0,005 0,007, under conditions of aeration and mixing is carried out maintaining the optimal viscosity in the range of 60 -10010-3Pass dolive sterile water or dolive hydrolyzed corn flour or a combination of both.Example 1. Vegetative AMF inoculum culture Str. kanamyceticus grown in kolbasy 0,25; soy flour 2,0; extract of corn 0,5; chalk 0,3; potato starch 2; animal fat is 0.1. The contents of one flask is transferred in the dry inoculant capacity 2000 l containing 800 l of nutrient medium of the following composition, wt. ammonium sulfate 0.3; the fat 1,25; chalk 0,3; corn flour 3,0; soy flour 2,0; salt 0,27; the extract of corn to 0.5, and is grown at a temperature of 27 1oC for 34 40 PM Contents inoculator passed in sowing machine with a capacity of 10 m3containing 5 m3the nutrient medium of the same composition as the environment in inoculate. The duration of the growing seed -24 hours the Contents of the sowing apparatus is passed into the fermenter with a capacity of 50 m3containing 30 m3fermentation medium of the following composition, wt.Ammonium sulfate 0,9
Animal fat 1,17
Iron sulfate 0,005
Corn flour 8,5
Flour soy 2,0
Zinc sulfate 0.003
The extract of corn 0,3
Propanol B-4 0,01
In the fermenter sets mode:
aeration from 0 to 44 h of growth flow increase speed from 0.25 to 1.0 volume 1 volume of medium per minute according to the following program:
Hours of growth, g air Consumption, the volume of the t 44 46 to 100 1,0
The temperature of cultivation 27 1oC, the rotational speed of the stirrer 3-1C.Upon reaching values of viscosity of the culture fluid 40 10-3Pass to 30-40 hours to produce the first topping 2 m3sterile water. The second and third dolive carry out the viscosity, support in the range of 60 - 10010-3A pass. The time between dolive 20 24 h, the amount of topping 2 m3.The concentration of the antibiotic kanamycin in culture liquid of 100 h is 3427 μg/ml, the amount of drain cultural liquid 39.5 m3, eat antibiotic to 136.4 kgIn a known method , the concentration of kanamycin in culture liquid of 100 h is 3100 µg/ml Volume of culture fluid 37,3 m3. Removal of antibiotic -115,6 kgExample 2. The medium composition, the growing seed, the cultivation conditions are the same as in example 1.The difference from example 1 is that when reaching a viscosity 4010-3Pass 30 40 h of growth produce first adding a hydrolysate of corn flour, the second topping produce the viscosity, keeping it in the range of 60 10010-3Pass through 20 24 hours 10 to 15 hours to drain topping produce sterile water to a volume of 2 m3according to the OEM topping hydrolyzed corn flour 3 m3.Indicators of biosynthesis of kanamycin at 100 h are: the concentration of kanamycin in the culture fluid 3858 μg/ml, the volume of the culture fluid is drained 40 m3, eat antibiotic 154,1 kgOperations previously known method  the parameters of the biosynthesis of 100 h of growth are 3100 µg/ml, the volume of 37.3 m3, eat antibiotic -115,6 kgLiterature
1. Chemistry of antibiotics. Publishing house of Academy of Sciences of the USSR, 1961, t, 1, S. 730. The method of producing the antibiotic kanamycin by culturing producer Streptomyces kanamyceticus in a nutrient medium containing sources of carbon, nitrogen, phosphorus, mineral salts under conditions of aeration and mixing, followed by separation of the target product, characterized in that in the process of cultivation carried out dolive hydrolyzed corn flour or sterile water in a quantity sufficient to maintain the viscosity level (60 100) 10-3Pas, the first topping is produced when reaching the viscosity of the culture fluid 40 10-3PA-C.
FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.
EFFECT: improved preparing method.
15 cl, 4 ex
FIELD: canned food industry.
SUBSTANCE: method involves preparing fruits; adding sugar solution; providing cavitational grinding of fruits in sugar solution and separating pulp under aseptic conditions; providing cavitational grinding by dispersing under atmospheric pressure in mixture of fruits and sugar solution of mycelium produced after extracting of Mortierella zychae micromycet biomass with non-polar liquefied gas; sequentially extracting biomass with water, alkaline, water, acid, water, alkaline, and water; introducing resultant solid residue into sugar solution in an amount of about 0.06% by weight of fruits.
EFFECT: reduced consumption of power for producing of canned food, improved quality of canned food owing to improved retention of color of basic material, and increased resistance to breaking of canned food during storage.
FIELD: food industry, confectionary industry.
SUBSTANCE: for the purpose to obtain jelly marmalade with a wide spectrum of vitamin activity and unconventional pleasant combination of organoleptic properties one should prepare the syrup by applying sugar, pectin and an aqueous extract of the mixture of baikhovi black tea, baikhovi green tea, Centaurea flowers, calendula flowers, mountain cranberry leaves, hibiscus flowers and dog rose fruit. While preparing it is necessary to supplement the syrup with citric acid, sodium lactate, "Mountain cranberry" and "Cream" aromatizing agents and a preparation obtained out of Mortierella exigua micromycete biomass by the preset technique to be further spread, formed, dried and packed as ready-to-use products.
EFFECT: higher efficiency.
SUBSTANCE: invention relates to technology of glycopeptides purification. Clarified liquid containing eremomycin is compacted with sulphuretted stirol-divinylbenzene cationite, elution is carried out and neutralised eluate is applied on the column with hydrophobic stirol divinylbenzene sorbent. After washing column with water, eremomycin is displaced with solution of isopropyl alcohol with addition of acetic acid and crystallisation of target product is carried out.
EFFECT: obtaining a pure product with a high output at a high process productivity.
6 cl, 3 ex
FIELD: microbiology; medicine.
SUBSTANCE: strain Amycolatopsis orientalis subsp.eremomycini All-Russian collection of industrial microorganisms Ac-807 is eremomycin producent and can be used as source of eremomycin in medicine for treatment of severe infections.
EFFECT: increase of eremomycin output.
2 tbl, 1 ex
SUBSTANCE: proposed strain of Nonomuraea roseoviolacea subsp carminata (Actinomadura carminata) BKIIM-S1081 is obtained as a result of multiple stage mutagenic effect of nitrimethylbiurets, methylnitrosoguanidine and gamma rays 60Co on the initial strain № All Russian collection of industrial microorganisms S-806 with subsequent extraction of mutants on media, containing streptomycin and daunorubicin as selective agents, and deposited under the number All Russian collection of industrial microorganisms S-1081. Carminomycine is obtained from biosynthesis of a strain producer, treatment of culture liquid with acetone and oxalic acid, alkalisation of the solution, extraction of antibiotic using chloroform, re-extraction of antibiotic using an aqueous buffer at pH=5.0, repeated extraction using organic solvents at pH=8.0, concentration of the extract, filtration through a neutral PTFE sorbent and extraction of antibiotic through precipitation from the solution in dichloromethane-hexane or petroleum-ether in form of a base.
EFFECT: increased output of carminomycine antibiotic, efficient in therapy of soft tissue sarcoma, lymphosarcoma, reticulosarcoma, chorionepithelioma, acute myeloblastoma and lymphoblastoma, chronic myeloid leukaemia (120 mcg in 1 ml of culture liquid).
2 cl, 1 tbl, 1 ex
FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and can be used in medical industry during production of antibacterial antibiotic lincomycin hydrochloride which is efficient in treating infections caused by gram-positive agents, including methicillin with stable and certain anaerobic bacteria. The method involves culturing a Streptomyces roseolus VKPM S-1082 strain, which is a lincomycin producer, on a culture medium with given composition, extraction of lincomycin with organic solvents from the native solution, extraction of lincomycin with subsequent isolation. The obtained extract is concentrated and crystallised to obtain lincomycin in hydrochloride form.
EFFECT: invention increases output of lincomycin.
2 cl, 1 tbl, 2 ex
SUBSTANCE: method involves cultivation of strain Streptoalloteichus cremeus subsp. tobramycini VKPM S-1084, producer of apramicyne. Emanation of apramicyne from culture fluid with sorption on carboxylic cationites followed by chromatographic purification with two-column method by stepwise elution with solutions (0.19-0.22)n of ammonia. The resulting eluate is concentrated in vacuum and precipitated with excess of acetone apramicyne in the form of sulfate.
EFFECT: increased output of apramicyne.
2 cl, 1 tbl, 2 ex
SUBSTANCE: Amycolatopsis fructiferi subsp. ristomycini 8765 strain comes from mutant transformation of Proactinomyces fructiferi var. ristomycini 5339 strain properties as a result of consequent three-step actions on the stock strain by methylnitrosoguanidine, ultraviolet and gamma-radiation of 60Co with following selection of mutated strain possessing increased antibiotic activity. Obtained strain differs from already known one with growth form on diagnostic agar media, carbon sources assimilation rate (saccharose, starch, rhamnose, and mannose) chromogenic ability and antibiotic activity which is 5 times higher of Proactinomyces fructiferi var. ristomycini 5339 strain activity and is equal to 2000 mcg/ml. Strain is deposited to All Russian Collection of Industrial Microorganisms, collection # S1085.
EFFECT: invention can be used in medical industry for antibacterial antibiotic ristomycin manufacture; ristomycin is effective in treatment of infections induced by gram-positive microorganisms.
SUBSTANCE: compound WS727713 is prepared by cultivation in a culture medium of Pseudonocardia FERM BP-7570 ray fungus strain producing such compound, and recovered from a culture fluid. The compound WS727713 is used as a melanocortin receptor modulator and applied for melanocortin receptor modulation while preparing a drug or a beauty preparation and preparing a pharmaceutical composition. Also, there are presented a pharmaceutical composition containing the compound WS727713, a method involving the introduction of specified compound in a human or animal body, and its application for treatment or prevention of the diseases responsive to melanocortin receptor regulation.
EFFECT: group of inventions exhibits the activity of a melanocortin receptor modulator.
12 cl, 3 dwg, 4 tbl, 1 ex