Bioreagent for purification of water and soil from oil pollution

 

(57) Abstract:

Usage: refers to applied Microbiology, may be used to clean the surface of the water and soil from spilled oil and petroleum products. Entity use: proposed bioreagent includes adapted to the hydrocarbon oil bacterial culture of Pseudomonas putida and biogenie diet containing nitrogen in ammonium form and phosphorus. When this bacterial culture and biogenic nutrition immobilized on gidrofobizirovannym peat. table 4.

The invention relates to applied Microbiology and can be used to clean the surface of the water and soil from spilled oil and petroleum products.

Known composition on the basis of nitrogen-lipophilic phosphate fertilizers, expanded perlite and bacterial preparation on the basis of the strain Pseudomonas putida 36.

The disadvantages of the known composition are limiting the scope of part only of the water surface, the complexity of the composition (the need for three components) and high cost structure, which includes the cost of production of dried bacterial preparation and the cost of imported lipophilic fertilizer UNIPOL ZAP-22).

The disadvantages of the known compositions are the limiting application in conditions of oxygen deficiency in water bodies with thick oil film, the high cost of obtaining dried bacterial preparation ( from 1 m3the culture fluid obtained 2 to 3 kg dry product), and also used as nutrient additive nitrogen in nitrate form can cause pollution of the environment with toxic substances.

Offers bioreagent for purification of water and soil from oil spills, including adapted to the hydrocarbon oil bacterial culture of Pseudomonas, which together with the components of biogenic nutrition, containing nitrogen in ammonium form and phosphorus, immobilized on gidrofobizirovannym peat in the following ratio, wt.

Bacterial culture of Pseudomonas 0,29 0,32

Sodium soap of synthetic fatty acids 3,70 of 4.38

Water-soluble aluminum salts and calcium 0,97 1,59

Ammonium salt of phosphoric acid 0,79 0,81

Peat Else

Media bacterial cells gidrofobizirovannym peat is the adsorbent of nefteproduktov the process of biodegradation of petroleum products. The presence of biogenic components supply of salts of nitrogen and phosphorus in bioreagent provides the best conditions for growth and oxidative activity of bacterial cells, and therefore does not require additional application of these components in the processing of oil-contaminated water and soil.

In the proposed bioreagent is much lower yields of bacterial cells that are capable of a long time to stay active in the area of biochemical oxidation (on the border of the oil-water). 1 m3the culture fluid can be obtained 1050 1080 kg bioreagent, which has a significant advantage compared with Luteolum".

All these features bioreagent ensure the intensification of the process of biodegradation of oil spills and allow to extend the scope of application of biological products, because they open the possibility of their use in flow and parinamah waters.

Example 1. To 20 g of dry peat, sifted through a sieve with cell diameter of 2 mm, poured 20 ml of 4% aqueous solution of sodium soap of FLC, stirred for 10 min, add 10 ml of 2% aqueous solution of aluminum sulfate, stirred for 10 min, add 20 ml of freshly prepared culture fluid in which pred Cup and dried in air. Get 21,3 g bioreagent.

Example 2. In the conditions of example 1 together with aluminum sulfate is used to 8.5 ml of 4% aqueous solution of calcium chloride. Gain of 21.2 g of bioreagent.

Example 3. To 500 g of sieved air-dried peat poured 590 ml of 4% aqueous solution of sodium soap of FLC, stirred for 15 min and add a mixture of 590 ml of the culture fluid and 43 ml of a 10% aqueous solution diammofosa, stirred for 15 min, add 213 ml of 4% aqueous solution of calcium chloride, stirred for 15 min and spread a thin layer (about 1 cm) on the glass surface, dried in the air. Get 530 g bioreagent.

For received bioreagents determine the buoyancy by the following method under conditions simulating the presence of chemicals in water, covered with a film of oil. 1 g of the reagent is placed in a flat-bottomed flask 100 ml add 50 ml of distilled water, close the tube, vigorously shaken for 10 seconds and add 1 ml of octane. Stoppered flask and incubated for 3 days, then with a spatula remove floating on the surface of the reagent in a beaker containing 30 ml of distilled water, filtered off on a suction filter, and dried in air precipitate to constant weight in a Petri dish, weighed. Plavuchem the th of examples 1 to 3 are given in table. 1. The optimum amount of sodium Soaps, alcohols, metal salts (calcium or aluminum) and diammofosa are given in table. 1, provides the greatest combination of buoyancy and maximum oxidizing activity bioreagents. The introduction of more diammofosa leads to a significant decrease in the hydrophobicity of the reagent, and a reduction in the content diammofosa causes decreased cell growth and consequently reduce nefteokislyayuschey ability bioreagent.

When using immobilized bioreagents accumulation of cells of the producer of the drug occurred within 3 days. reaching a titer of 4 days. and on the 7th day. their content was equal in the experiments with the prototype (table.1).

Comparative evaluation of the effectiveness of the proposed bioreagents relative to the prototype were carried out in a series of experiments on water and soil in the presence of petroleum products. In all examples, the dose of drug was carried out based on 1 g of the product: backpropagate "Poutical" 30 mg, bioreagent 60 mg.

The decomposition efficiency of petroleum products was estimated by the residual content method gravimetry in relation to the original and the coefficient of biochemical activity kn(%), THE C19; i C20isoprenoid alkanes;

n C17; n C19normal alkanes.

Used for the analysis chromatograph "Chrome 5", equipped with a flame ionization detector.

During the biochemical oxidation decreases the total number of oil and increasing the value of Kithe higher this ratio, the more intensive the process. The efficiency of the oxidation process (Kn) of the proposed bioreagents was 85.5 94%

Example 4. In a flask with 5.0 l of water was made of different types of petroleum products a certain concentration

N 1 5.0 g of crude oil;

N 2 16.0 g of slurry waste motor plant;

N 3 6.0 g of diesel fuel.

Then were treated with bioreagents or bacterial preparation "Poutical".

The efficiency of the oxidation process was assessed on a 10 day method gravimetry in relation to the original. The proposed bioreagent provides effective biodegradation film layer of petroleum products in the absence of oxygen (table. 2).

Example 5. Evaluation of the effectiveness of bioreagent at different depths was conducted on an oil film in the liquid column. For this purpose the cylinders with a height of 50 cm, diameter 60 mm made of 1.0 l of water and 5 ml of oil. Treatment is of ducenta preparation and determination of the coefficient of biochemical activity (Kn) oil was carried out at 5, 20, 40 cm (PL. 3).

The ability of bioreagent to stay on the surface of the oil film and the property of immobilized microorganisms that can make you retain optimal concentration of microbial cells on contact with water, oil, and ensures a higher degree of biochemical activity.

Example 6. The ability of bioreagent to oxidation of petroleum hydrocarbons in soil was tested in the experiment in the grey forest soil.

3 kg of grey forest soil were introduced 300 g of crude oil were processed by bioreagents N 3 or bookreporter in the calculated dose. After 30, 45 and 90 days was determined by the efficiency of the oxidation process gravimetric method. The test results are given in table. 4.

Thus, the proposed bioreagent provides effective biodegradation of crude oil and petroleum products in soil and water bodies, including those where there are no conditions for the development of aerobic processes.

Bioreagent for purification of water and soil from oil spills, including adapted to the hydrocarbon oil bacterial culture of Pseudomonas components and nutrient supply, characterized in that as component of biogenic pemoline salt of phosphoric acid, when this bacterial culture together with components of the nutrient supply immobilized on gidrofobizirovannym peat in the following ratio, wt.

Bacterial culture of Pseudomonas 0,29 0,32

Sodium soap of synthetic fatty acids 3,70 of 4.38

Water-soluble aluminum salt or calcium 0,97 1,59

Ammonium salt of phosphoric acid 0,79 0,81

Peat Rest

 

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