The way to select phenoxymethylpenicillin acid


(57) Abstract:

Usage: biotechnology, production of antibiotics. The inventive method of allocating phenoxymethylpenicillin acid involves filtering the culture fluid, the deposition from the native solution of the technical phenoxymethylpenicillin acid, its dissolution in acetone to a concentration of 100,000 - 200,000 IU/ml, subsequent filtration and isolation of the target product. 2 C.p. f-crystals,

The invention relates to biotechnology and relates to a method of obtaining biologically active substances (BAS), namely to receive the dentists used as an intermediate product to obtain a semisynthetic B-lactam antibiotics and chemotherapy of bacterial infections.

Known methods for producing dentists, consisting in the extraction of the antibiotic from native solution of organic solvents, mainly butyl acetate, and the deposition of the intermediate product from the extract by the action of potassium acetate or potassium with subsequent selection FMP acid (U.S. Pat. Russia N 17264796, CL C 07 D 499/16; U.S. Pat. Czechoslovakia N 124845; U.S. Pat. Czechoslovakia N 127853; industrial regulations on production phenoxymethylpenicillin process, the use of large amounts of organic solvents, the use of various types of raw materials.

In addition, the product (salt and SEF acid) obtained by the above methods, contains an admixture phenoxyalkanoic acid, which produces a lower output when receiving 6-aminopenicillanic acid.

Known such methods of obtaining dentists from native solution-based deposition of technical FMP acid by the introduction of a mineral acid in sodium solution, followed by dissolving technical acid in an organic solvent and precipitation from the solution obtained dentists To salts (U.S. Pat. USA N 4354971, class C 07 D 499/18; A. S. Czechoslovakia N 185103, class C and 121 D 9/12).

The closest in technical essence and the achieved result is a method for dentists outlined in the ed.St. Czechoslovakia N 185103 consisting of using as the source of raw materials of technical FMP allocated from native solution by the action of hydrochloric acid. Dried technical product twice suspension in butyl acetate, resulting in a gain SEF solution in butyl acetate with activity 31,600 units/ml of the obtained solution Clevo is whether SEF from reextract.

The disadvantages of this method are the multistage process, significant costs for raw materials, environmental pollution due to the use of large volumes of organic solvents (butyl acetate, butanol).

These disadvantages are completely eliminated in the present method of selection of dentists.

The aim of the invention is to increase the output and quality dentists, improving the environment, reducing the cost of production.

This goal is achieved by what is known of the process is eliminated several stages, leading to the loss of the antibiotic (dual suspension technical acid in butyl acetate, reextracted antibiotic aqueous solution of potassium acetate, followed by salting out K-salt).

Distinctive features of the proposed method is used for dissolving technical FMP-acid organic solvent miscible with water, for example acetone, and crystallization FMP acid from water-acetone mixture.

Example 1.1.Dissolution technical FMP acid.

58 g technical FMP acid and the -20oC, filtered off from nerastvorimogo precipitate protein impurities, wash the precipitate with 70 ml of acetone.

Get 710 ml acetone concentrate dentists activity 113718 μg/ml, the content of dentists 80,74 million units of Output at the stage of dissolution 96%

2. Clarification of acetone concentrate.

To the resulting clarified concentrate FMP added 16.5 g of activated charcoal brand OS-And stirred the reaction mass 20 min, filtered off from the coal, the coal is washed on the filter with 50 ml of acetone, receive 750 ml clarified acetone concentrate activity 105, 506 ág/ml, the content of dentists of 79.13 million units of chromaticity of 0.18.

The output stage of clarification 98%

3. Crystallization FMP acid.

To 750 ml of clarified acetone concentrate add 1500 ml of demineralized distilled water, cooled the reaction mass to 5 - 10oC and maintained at this temperature and stirring for 2 to 4 h until the residual activity of the stock solution of no more than 1000 IU/ml

This activity values of the reaction mass is filtered, the residue washed with 200 ml of desalted (distillirovanna the capacity of 1680 μg/mg, the content of the SEF 74,38 million units Output stage 94,0% yield from technical FMP acid 887,44%

Example 2. Analogously to example 1, but the dissolution technical FMP-acid lead from the calculate activity acetone concentrate approximately 200,000 ág/ml and used at the stage of crystallization of 4 volumes of water relative to the volume of acetone concentrate.

Example 3. Analogously to example 1, but using at the stage of dissolution of isopropyl alcohol.

Example 4. Dissolution technical FMP acid analogously to examples 3,2 (isopropyl alcohol, the concentration of antibiotic 200,000 ág/ml).

Example 5. Analogously to example 1, but using at the stage of dissolving butyl alcohol.

Example 6. Dissolution technical FMP acid analogously to examples of 5.2 (butyl alcohol, the concentration of antibiotic 200,000 ág/ml).

1. The way to select phenoxymethylpenicillin acid comprising filtering the culture fluid, the deposition from the native solution of the technical phenoxymethylpenicillin acid, its dissolution in an organic solvent, filtering and selection of the target product, characterized in that the dissolution of the technical phenoxymethylpenicillin acid Prov is the amount of organic solvent, miscible with water, use acetone.

3. The method according to p. 1, characterized in that the dissolution of the acid in an organic solvent is carried out until the concentration of 100000 200000 IU/ml


Same patents:

The invention relates to biotechnology and relates to a method of extraction of biologically active substances (BAS)

The invention relates to a new connection, which is called "Thiomarind", as well as the method of preparation thimerisol, including enzyme using microorganism biological genus Alteromonas and especially the model of a new species of Alteromonas rava marked SANK 73390, which is novel in itself and is part of the present invention
The invention relates to medicine and pharmacology, more precisely to a method of allocating natural antibiotics directly from the fermentation media, without prior separation of biomass and provide a filtered fermentation medium with the dissolved antibiotic so-called native solution

The invention relates to a new antitumor chromophore derived from cedarcide antibiotic
The invention relates to the microbiological industry, in particular the production of antibiotics

The invention relates to biotechnology and relates to a new strain of the actinomycete Streptomyces pluricolorescens isolated from the marine domain precipitation, producer of the new polypeptide antibiotic palmeranian possessing inhibitory activity against gram-positive bacteria

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

EFFECT: improved preparing method.

15 cl, 4 ex

FIELD: canned food industry.

SUBSTANCE: method involves preparing fruits; adding sugar solution; providing cavitational grinding of fruits in sugar solution and separating pulp under aseptic conditions; providing cavitational grinding by dispersing under atmospheric pressure in mixture of fruits and sugar solution of mycelium produced after extracting of Mortierella zychae micromycet biomass with non-polar liquefied gas; sequentially extracting biomass with water, alkaline, water, acid, water, alkaline, and water; introducing resultant solid residue into sugar solution in an amount of about 0.06% by weight of fruits.

EFFECT: reduced consumption of power for producing of canned food, improved quality of canned food owing to improved retention of color of basic material, and increased resistance to breaking of canned food during storage.

3 ex

FIELD: food industry, confectionary industry.

SUBSTANCE: for the purpose to obtain jelly marmalade with a wide spectrum of vitamin activity and unconventional pleasant combination of organoleptic properties one should prepare the syrup by applying sugar, pectin and an aqueous extract of the mixture of baikhovi black tea, baikhovi green tea, Centaurea flowers, calendula flowers, mountain cranberry leaves, hibiscus flowers and dog rose fruit. While preparing it is necessary to supplement the syrup with citric acid, sodium lactate, "Mountain cranberry" and "Cream" aromatizing agents and a preparation obtained out of Mortierella exigua micromycete biomass by the preset technique to be further spread, formed, dried and packed as ready-to-use products.

EFFECT: higher efficiency.

FIELD: chemistry.

SUBSTANCE: invention relates to technology of glycopeptides purification. Clarified liquid containing eremomycin is compacted with sulphuretted stirol-divinylbenzene cationite, elution is carried out and neutralised eluate is applied on the column with hydrophobic stirol divinylbenzene sorbent. After washing column with water, eremomycin is displaced with solution of isopropyl alcohol with addition of acetic acid and crystallisation of target product is carried out.

EFFECT: obtaining a pure product with a high output at a high process productivity.

6 cl, 3 ex

FIELD: microbiology; medicine.

SUBSTANCE: strain Amycolatopsis orientalis subsp.eremomycini All-Russian collection of industrial microorganisms Ac-807 is eremomycin producent and can be used as source of eremomycin in medicine for treatment of severe infections.

EFFECT: increase of eremomycin output.

2 tbl, 1 ex

FIELD: biology.

SUBSTANCE: proposed strain of Nonomuraea roseoviolacea subsp carminata (Actinomadura carminata) BKIIM-S1081 is obtained as a result of multiple stage mutagenic effect of nitrimethylbiurets, methylnitrosoguanidine and gamma rays 60Co on the initial strain № All Russian collection of industrial microorganisms S-806 with subsequent extraction of mutants on media, containing streptomycin and daunorubicin as selective agents, and deposited under the number All Russian collection of industrial microorganisms S-1081. Carminomycine is obtained from biosynthesis of a strain producer, treatment of culture liquid with acetone and oxalic acid, alkalisation of the solution, extraction of antibiotic using chloroform, re-extraction of antibiotic using an aqueous buffer at pH=5.0, repeated extraction using organic solvents at pH=8.0, concentration of the extract, filtration through a neutral PTFE sorbent and extraction of antibiotic through precipitation from the solution in dichloromethane-hexane or petroleum-ether in form of a base.

EFFECT: increased output of carminomycine antibiotic, efficient in therapy of soft tissue sarcoma, lymphosarcoma, reticulosarcoma, chorionepithelioma, acute myeloblastoma and lymphoblastoma, chronic myeloid leukaemia (120 mcg in 1 ml of culture liquid).

2 cl, 1 tbl, 1 ex

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology and can be used in medical industry during production of antibacterial antibiotic lincomycin hydrochloride which is efficient in treating infections caused by gram-positive agents, including methicillin with stable and certain anaerobic bacteria. The method involves culturing a Streptomyces roseolus VKPM S-1082 strain, which is a lincomycin producer, on a culture medium with given composition, extraction of lincomycin with organic solvents from the native solution, extraction of lincomycin with subsequent isolation. The obtained extract is concentrated and crystallised to obtain lincomycin in hydrochloride form.

EFFECT: invention increases output of lincomycin.

2 cl, 1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: method involves cultivation of strain Streptoalloteichus cremeus subsp. tobramycini VKPM S-1084, producer of apramicyne. Emanation of apramicyne from culture fluid with sorption on carboxylic cationites followed by chromatographic purification with two-column method by stepwise elution with solutions (0.19-0.22)n of ammonia. The resulting eluate is concentrated in vacuum and precipitated with excess of acetone apramicyne in the form of sulfate.

EFFECT: increased output of apramicyne.

2 cl, 1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: Amycolatopsis fructiferi subsp. ristomycini 8765 strain comes from mutant transformation of Proactinomyces fructiferi var. ristomycini 5339 strain properties as a result of consequent three-step actions on the stock strain by methylnitrosoguanidine, ultraviolet and gamma-radiation of 60Co with following selection of mutated strain possessing increased antibiotic activity. Obtained strain differs from already known one with growth form on diagnostic agar media, carbon sources assimilation rate (saccharose, starch, rhamnose, and mannose) chromogenic ability and antibiotic activity which is 5 times higher of Proactinomyces fructiferi var. ristomycini 5339 strain activity and is equal to 2000 mcg/ml. Strain is deposited to All Russian Collection of Industrial Microorganisms, collection # S1085.

EFFECT: invention can be used in medical industry for antibacterial antibiotic ristomycin manufacture; ristomycin is effective in treatment of infections induced by gram-positive microorganisms.

1 ex

Compound ws727713 // 2407785

FIELD: medicine.

SUBSTANCE: compound WS727713 is prepared by cultivation in a culture medium of Pseudonocardia FERM BP-7570 ray fungus strain producing such compound, and recovered from a culture fluid. The compound WS727713 is used as a melanocortin receptor modulator and applied for melanocortin receptor modulation while preparing a drug or a beauty preparation and preparing a pharmaceutical composition. Also, there are presented a pharmaceutical composition containing the compound WS727713, a method involving the introduction of specified compound in a human or animal body, and its application for treatment or prevention of the diseases responsive to melanocortin receptor regulation.

EFFECT: group of inventions exhibits the activity of a melanocortin receptor modulator.

12 cl, 3 dwg, 4 tbl, 1 ex