The method of producing ergosterol

 

(57) Abstract:

Usage: the invention relates to the field of biotechnology, and more specifically to methods of obtaining vitamins. The inventive method involves the production of ergosterol from yeast by processing a mixture containing 25-30% sodium hydroxide, 15-50% water and 25-50% of isopropyl alcohol extraction of ergosterin organic solvent selected from the group of substances comprising: isopropyl alcohol, distillation of isopropyl alcohol, a mixture of isopropyl alcohol and its distillate in a ratio of 1:1. table 2.

The invention relates to the field of biotechnology, and more specifically to methods of obtaining vitamins, namely a process for the production of ergosterol.

Vitamin D2get technology, including the cultivation of yeast Sacch. cerevisiae, the allocation of them ergosterol, photoisomerization of the latter under the action of UV-irradiation and selection of vitamin D2from photomay [1]

The biggest problem causes the stage of selection of ergosterol from yeast.

Known methods of extraction of ergosterol, its extraction from yeast, processed COHN, dichloroethane or benzene, with subsequent distillation of the solvent [2,3]

The disadvantages of these methods, I is the technology of obtaining ergosterol, includes treatment of yeast with a water-alcohol solution of KOH in boiling, after which the mixture is filtered, the filtrate concentrated and crystallized, getting ergosterol raw, which is optionally crystallized from 65% ethyl alcohol [1]

Ergosterol has a melting point of not less than 158oC a purity of 88% and output depending on the composition of the yeast from 73 to 81%

The disadvantages of the prototype are the high consumption of ethyl alcohol, the need for its rectification, the duration of solvolysis (not less than 6 h), the need for filtering of neomillennial residues of yeast and low output.

The purpose of the invention to develop more effective and manufacturable process.

It was found that the efficiency of the process can be improved by conducting solvolysis mixture containing 25-50% isopropyl alcohol (IPA), 15-50% water and 25-35% sodium hydroxide, followed by extraction of ergosterol isopropyl alcohol in the ratio to the original yeast is not less than 0.6:1. A higher ratio is practically not lead to increased output, demand additional expenses for the selection of the target product.

As solvent in the extraction step can be used as Tova what I solvolysis and extraction in the literature for obtaining vitamin D2not described, which confirms the compliance with the criterion of "world novelty."

The study of the new conditions of solvolysis and extraction shows that the application of the new formula allows, in contrast to the use of ethyl alcohol, to create a two-phase system that eliminates stage filtration and loss of ergosterol, and, in contrast to the use of higher alcohols, such as butyl, managed in the process to avoid emulsification and foaming. Application for the extraction of the distillate IPS simplifies the process and increases the yield of ergosterol to 85-90%

The mentioned set of features not previously found, leads to unexpected results, in particular, to increase the yield of ergosterol to 90% which indicates compliance with a criterion of "inventive step".

Example 1.

A 5-liter flask with stirrer, thermometer and reflux condenser load of 2.25 kg of yeast, 450 ml of an aqueous NaOH solution containing 260 g of dry alkali and 250 g technical IPS. The mixture is boiled for 4 hours with stirring, cooled and add 1.5 kg technical IPS or its distillate, stirred for 30 min, defend and separating the upper layer of the extract, which is filtered from neznachytelnaya), which is boiled with 10-fold volume of isopropyl alcohol, filtered, the solvent is distilled off, the residue is dried in vacuum.

The product contains 88-92% of ergosterol with tpl.159-160oC. Yield 90%

Example 2.

In the conditions of example 1 were solvolysis various alkaline mixtures. The results obtained are given in table. 1.

Example 3.

In the conditions of example 1 were extracted and IPA distillation of isopropyl alcohol. The results obtained are given in table. 2.

The application of new technology for production of ergosterol to produce vitamin D2"Farmakon" managed by reducing energy and labor costs, cost of reagents, increasing the output of ergosterol to get ready the economic effect of more than 4 million rubles (price 1992).

Literature

1. A. I. Vorobyov. Microbiological synthesis of vitamins. M MSU, 1982, S. 139-150.

2. HFG. 1971, No. 9, S. 41.

3. Berezovsky C. M. Chemistry of vitamins. M Food industry, 1973, S. 130.

The method of producing ergosterol from yeast by treating them with an alkaline reagent, extraction of ergosterol organic solvent and selection of the target product, characterized in that as the alkaline reagent, a mixture, startelem, selected from the group of substances including isopropyl alcohol, distillation of isopropyl alcohol, obtained at the stage of evaporation, the mixture of isopropyl alcohol and its distillate in a ratio of 1 to 1.

 

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