Producer antitumor melaninogenica drug "stromelysin"
(57) Abstract:Usage: biotechnology, Microbiology, medicine, obtaining substances anticarcinogenic actions. The inventive use of a mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer melaninogenica drug with high antitumor effect and does not exhibit toxicity. The invention relates to biotechnology, in particular the production of biologically active substances of antitumor action.Known microorganism producer melaninogenica substances belonging to Streptomyces melanogenes.However, melanin produced by them do not show antitumor properties 
There are various drugs having anti-tumor activity, used in the treatment of cancer: benzoate, cyclophosphamide, adriablastin (lung cancer and breast cancer), karminomitsin, vincristine, methotrexate, thiotepa, Ftorafur (breast cancer) and others  they are All toxic in therapeutic doses, side effects and contraindications.The advantages and technical effects of the invention consists in the detection, not inherent in the known means of similar purpose, namely, the application of the known mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica of the drug. Its properties provide effective treatment of cancer.The inventive use of a mutant strain 1-365 Nadsoniella nigra var. hesuelica as unlimited and legkovozvodimih source of melanin with antitumor properties and non-toxic, which makes its practical use is particularly valuable.Mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica was obtained under the action of x-ray radiation (250 crad) on the cells of the original Antarctic strain Nadsoniella nigraa var. hesuelica 365 
Strain 1-365 characterized by altered cell wall and are able to accumulate pigment in environments in neutral, weakly acidic and weakly alkaline pH values. The pigment is associated with the cell wall, but is relatively easily removed by the alkaline solution. Electron microscopy showed quite loose packing of the pigment granules in the cell wall, part of which would clusives it 
The process is a violation of cell biomass Department of pigment deposition at acidic pH values and transfer in water-soluble form 
An important characteristic of the obtained product is the presence in the structure of melanin strain 1-365 stable free radicals in high concentrations in the cellular melanin strain 1-365 81018/g dry.prophetic. in the extracellular 31018/g dry. prophetic.Derived from strain 1-365 water-soluble melaninogenicus drug called "Stromelysin". He became the object of the research is to identify new valuable properties, non-toxicity and expression of antitumor action. Discovered the useful properties of Astromania were not previously known and is not described in the literature.Usage data obtained from strain 1-365 Nadsoniella nigra var. hesuelica melaninogenica product for the treatment of volunteers with various forms of cancer are listed below.Strain 1-365 cultivated by well-known methods in the conditions of aeration and mixing in liquid mineral medium of the following composition, g/l: KN2RHO4Oh,1; l20,03; gSO40,05; Nl 0,05 with glucose (2%), yeast autolysate (1%) at 26-28oWith in a week.The initial value of pH is adjusted to pH 3.Water-soluble drug "Stromelysin" obtained by dissolving obtained as described above, the precipitate in a small amount of ammonia water (25-cent solution of ammonia), followed by a soft evaporation of ammonia and water.The dry preparation "Stromelysin" appearance is a brown-black crystals with a bright shimmering Shine. Its physico-chemical properties correspond to the indicators listed in the above sources. The resulting powder was used to prepare aqueous solutions of desired concentration of melanin.Preparation from strain 1-365 showed high cytotoxic activity against human cancer, in particular, cell lines of lung cancer (85% of deaths) and breast cancer (two lines, more than 90%), as well as in the treatment of patients-volunteers.Example 1. Patient A. colorectal cancer, the daily dose inside 60-10 mg, healthy year and a half.Example 2. Patient S. oral cancer, daily goat food improvement in blood counts.The drug strain 1-365 nontoxic (experiments on cell cultures and on the author C. P. Order). Side effects were observed.Thus, from comparison with known means the same destination can be seen that the use of the drug melanin from strain 1-365 Nadsoniella nigra var. hesuelica avoids adverse toxic effects at effective mainly antitumor action. It follows that the strain Nadsoniella nigra var. hesuelica 1-365 is a valuable producer of highly effective anticancer agents. The application of strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica of the drug.
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: the strain-producer is isolated from soft coral Sinularia sp. relating to species Aspergillus fumigatus and deposited in Collection of Marine Microorganisms of Pacific institute of bioorganic chemistry DVO RAN (KMM TIBOKH) at № 4631. Method for preparing indole alkaloids involves surface solid-phase culturing the indicated strain on nutrient medium, milling mycelium and medium, two-fold extraction of prepared mixture with system chloroform - ethanol (2:1), concentrating extract, its chromatography on column with silica gel and the following separation of indole alkaloids by HPLC method. Method based on applying this strain provides enhancing yield of indole alkaloids. Invention can be used in preparing indole alkaloids eliciting an anti-tumor activity.
EFFECT: improved method for preparing, valuable medicinal properties of indole alkaloids.
4 cl, 1 tbl, 2 ex
FIELD: biotechnology, medicine, antibiotics.
SUBSTANCE: invention proposes to the new compound amycomycin of the molecular formula C65H115NO18 (structural formula is given on the invention claim) that shows an antibacterial activity. Amycomycin, its pharmaceutically acceptable salts and derivatives in all their stereoisomeris and tautomeric forms can be obtained by culturing microorganism Amycolatopsis sp. ST 101170 (DSM 12216) under aerobic conditions on the nutrient medium containing the necessary nutrient components. The end product is isolated and purified and converted if necessary to its pharmacologically acceptable salt, ester, ether and other chemical derivatives and eliciting the same spectrum of antibacterial activity. Amycomycin is a component of the pharmaceutical composition eliciting an antibacterial activity. Amycomycin acts as an antibiotic. Invention provides inhibition of microorganisms with resistance to vancomycin and teicoplanin used in treatment of infections caused by Staphylococcus aureus.
EFFECT: improved preparing method, valuable medicinal properties of amycomycin.
7 cl, 2 tbl, 4 ex
FIELD: biotechnology, in particular production of melanin which represents pigment of high antioxidant and anti-tumor activity.
SUBSTANCE: claimed method includes providing by selection of yeast strain Nadsoniella nigra which under deep cultivation accumulates up to 25 g/l of yeast biomass after 96 hours from cultivation starting. Melanin is isolated both from yeast biomass (intracellular melanin) and from cultural liquid (extracellular melanin). Intracellular melanin is isolated by extraction with alkali solution followed by melanin deposition with hydrochloric acid. To isolate extracellular melanin cultural liquid is treated with ethanol followed by melanin deposition with hydrochloric acid.
EFFECT: method if increased effectiveness.
FIELD: biotechnology, biochemistry, enzymes, microbiology.
SUBSTANCE: invention proposes a method for microbiological oxidation of N-, O- or S-heterocyclic mono- or multinuclear aromatic compounds. The method involves culturing the recombinant microorganism that expresses cytochrome P-450-dependent monooxygenase BM-3 from Bacillus megaterium with amino acid sequence represented in SEQ ID NO:2 that comprises at least one functional mutation in region 86-88 and, if necessary, at least one functional mutation in one of regions 73-82, 172-224, 39-43, 48-52, 67-170, 300-335 and 352-356. The prepared oxidation product is isolated from medium, Invention provides carrying out oxidation of organic compounds with enhanced degree of effectiveness.
EFFECT: enhanced effectiveness of enzymes.
17 cl, 1 tbl, 7 ex
FIELD: organic chemistry, biotechnology.
SUBSTANCE: invention refers to biotechnology and can be applied in food industry for sweeteners production. Method of indole-3-pyruvic acid production includes indole-3-pyruvic acid production with enzyme catalyzing transformation of tryptophane to indole-3-pyruvic acid and deaerating treatment or oxygen-removing treatment in acid conditions. And enzyme made of microorganism characterized with amino-acid oxidase activity and catalase activity is used. 4-(indole-3-ilmethyl)-4-hydroxy-2-oxoglutaric acid is made of indole-3-pyruvic acid and oxaloacetic acid and pyruvic acid. Methods are easy realized and enable to produce high yield products.
EFFECT: production of indole-3-pyruvic acid.
15 cl, 17 tbl, 4 ex
SUBSTANCE: invention relates to a method of producing (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid, which includes enantioselective enzymatic reduction of an aryl ketone in a reaction mixture containing oxidoreductase and carbamination of the obtained alcohol.
EFFECT: obtaining (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid.
19 cl, 1 tbl, 5 ex
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
EFFECT: valuable medicinal properties of strain.
5 cl, 8 dwg, 10 ex
FIELD: biotechnology, microbiology, agriculture.
SUBSTANCE: the strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
EFFECT: valuable properties of strain.
3 tbl, 2 ex