Producer antitumor melaninogenica drug "stromelysin"


(57) Abstract:

Usage: biotechnology, Microbiology, medicine, obtaining substances anticarcinogenic actions. The inventive use of a mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer melaninogenica drug with high antitumor effect and does not exhibit toxicity.

The invention relates to biotechnology, in particular the production of biologically active substances of antitumor action.

Known microorganism producer melaninogenica substances belonging to Streptomyces melanogenes.

However, melanin produced by them do not show antitumor properties [1]

There are various drugs having anti-tumor activity, used in the treatment of cancer: benzoate, cyclophosphamide, adriablastin (lung cancer and breast cancer), karminomitsin, vincristine, methotrexate, thiotepa, Ftorafur (breast cancer) and others [2] they are All toxic in therapeutic doses, side effects and contraindications.

The advantages and technical effects of the invention consists in the detection, not inherent in the known means of similar purpose, namely, the application of the known mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica of the drug. Its properties provide effective treatment of cancer.

The inventive use of a mutant strain 1-365 Nadsoniella nigra var. hesuelica as unlimited and legkovozvodimih source of melanin with antitumor properties and non-toxic, which makes its practical use is particularly valuable.

Mutant strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica was obtained under the action of x-ray radiation (250 crad) on the cells of the original Antarctic strain Nadsoniella nigraa var. hesuelica 365 [3]

Strain 1-365 characterized by altered cell wall and are able to accumulate pigment in environments in neutral, weakly acidic and weakly alkaline pH values. The pigment is associated with the cell wall, but is relatively easily removed by the alkaline solution. Electron microscopy showed quite loose packing of the pigment granules in the cell wall, part of which would clusives it [4]

The process is a violation of cell biomass Department of pigment deposition at acidic pH values and transfer in water-soluble form [2]

An important characteristic of the obtained product is the presence in the structure of melanin strain 1-365 stable free radicals in high concentrations in the cellular melanin strain 1-365 81018/g dry.prophetic. in the extracellular 31018/g dry. prophetic.

Derived from strain 1-365 water-soluble melaninogenicus drug called "Stromelysin". He became the object of the research is to identify new valuable properties, non-toxicity and expression of antitumor action. Discovered the useful properties of Astromania were not previously known and is not described in the literature.

Usage data obtained from strain 1-365 Nadsoniella nigra var. hesuelica melaninogenica product for the treatment of volunteers with various forms of cancer are listed below.

Strain 1-365 cultivated by well-known methods in the conditions of aeration and mixing in liquid mineral medium of the following composition, g/l: KN2RHO4Oh,1; l20,03; gSO40,05; Nl 0,05 with glucose (2%), yeast autolysate (1%) at 26-28oWith in a week.

The initial value of pH is adjusted to pH 3.

Water-soluble drug "Stromelysin" obtained by dissolving obtained as described above, the precipitate in a small amount of ammonia water (25-cent solution of ammonia), followed by a soft evaporation of ammonia and water.

The dry preparation "Stromelysin" appearance is a brown-black crystals with a bright shimmering Shine. Its physico-chemical properties correspond to the indicators listed in the above sources. The resulting powder was used to prepare aqueous solutions of desired concentration of melanin.

Preparation from strain 1-365 showed high cytotoxic activity against human cancer, in particular, cell lines of lung cancer (85% of deaths) and breast cancer (two lines, more than 90%), as well as in the treatment of patients-volunteers.

Example 1. Patient A. colorectal cancer, the daily dose inside 60-10 mg, healthy year and a half.

Example 2. Patient S. oral cancer, daily goat food improvement in blood counts.

The drug strain 1-365 nontoxic (experiments on cell cultures and on the author C. P. Order). Side effects were observed.

Thus, from comparison with known means the same destination can be seen that the use of the drug melanin from strain 1-365 Nadsoniella nigra var. hesuelica avoids adverse toxic effects at effective mainly antitumor action. It follows that the strain Nadsoniella nigra var. hesuelica 1-365 is a valuable producer of highly effective anticancer agents.

The application of strain 1-365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica of the drug.


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