Producer antitumor melaninogenica drug "stromelysin"
(57) Abstract:Usage: biotechnology, medicine, Microbiology, obtaining substances anticarcinogenic actions. The inventive application of the Antarctic strain 365 black yeast Nadsoniella nigra var.hesuelica as producer melaninogenica drug with high antitumor effect and does not exhibit toxicity. The invention relates to biotechnology, in particular the production of biologically active substances of antitumor action.Known microorganism producer melaninogenica substances belonging to Streptomyces melanogenes. However, the resulting melanin does not possess antitumor properties 
There are various drugs having anti-tumor activity, used in the treatment of cancer: benzoate, cyclophosphamide, adriablastin (lung cancer), fluorouracil (tumors of the colon), olivomycin, vincristine, prospidin (melanoma) and others  All of them are known to have harmful side effects, contraindications, and, in addition, require intravenous or intramuscular use.Advantage or technical effect image the properties, not inherent in the known means of similar purpose, namely, the application of the known strain 365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica drug whose properties provide effective treatment of cancer.Summary of the invention the use of this strain Antarctic black yeast Nadsoniella nigra var. hesuelica 365 as unlimited and legkovozvodimih source of melanin with antitumor properties and non-toxic, which makes its practical use is particularly valuable.Strain 365 Antarctic black yeast Nadsoniella nigra var.hesuelica selected from the sample substrate Antarctic Islands Haswell and is known 
Produced by strain 365 black-brown pigment belonging to the group of melanin, is localized in the form of granules in the cell wall, making it quite dense outer layer of structurally and chemically closely related to other elements of the cell wall 
The process of selection of the target melaninogenica product from the biomass of cells of strain (and its main characteristics are described and include the destruction of the cells of the biomass, the Department piikoi resulting product is the presence in the structure of melanin strain 365 stable free radicals at a high concentration (1019/g) dry matter 
Get melaninogenicus drug called "Stromelysin". Discovered the useful properties of Astromania" lack of toxicity, antitumor effect were not previously known and is not described in the literature.Usage data obtained from strain 365 Nadsoniella nigra var. heselica melaninogenica product for the treatment of volunteers with various forms of cancer are listed below.Strain 365 cultivated by well-known methods in the conditions of aeration and mixing in liquid mineral medium of the following composition, g/l: NK2RHO40,1; l20,03; gSO40,05; NaCl, 0,05 with glucose (2%) and liquid yeast autolysate (1%) at 26-28oC and pH 3.5 during the week. The biomass was separated by centrifugation and repeatedly washed with distilled water, then biomass autoclave at 0.5 ATM with 0.5 n Paon within 3 hours of Cell mass is removed by centrifugation, centrifugal acidified with concentrated Hcl to pH 2-1. Sediment in the form of black flakes separated by centrifugation and repeatedly washed with distilled water. To obtain water-soluble drug residue dried and dissolved in a small amount of ammonia water (25% solution Ammi is Ernie crystals with bright shimmering Shine. Its physico-chemical properties correspond to the indicators listed in the above sources. The resulting powder was used to produce aqueous solutions of desired concentration of melanin.Obtained from strain 365 drug showed high activity against human cancer cells, in particular cell line carcinoma of the colon (80%), cell lines of lung cancer (89%), as well as in the treatment of patients-volunteers:
Example 1. Patient Century, lung cancer, ingestion 40-10 mg daily dose, recovery 2 years.Example 2. Patient I. (physician) melanoma, 1U stage, ingestion 60-10 mg daily dose, and locally to the lymph nodes, recovery 1 year 7 months old. high working capacity.The drug is non-toxic (experiments on cell cultures and on the author C. P. Order). Side effects were observed.Thus, from comparison with known means the same destination can be seen that the use of the drug melanin from strain 365 Nadsoniella nigra var.hesuelica avoids toxic side steps for effective mainly antitumor action. It follows that the strain Nadsoniella var. hesuelica 365 is a producer of high-performance protivoopujolevoe antitumor melaninogenica of the drug.
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: the strain-producer is isolated from soft coral Sinularia sp. relating to species Aspergillus fumigatus and deposited in Collection of Marine Microorganisms of Pacific institute of bioorganic chemistry DVO RAN (KMM TIBOKH) at № 4631. Method for preparing indole alkaloids involves surface solid-phase culturing the indicated strain on nutrient medium, milling mycelium and medium, two-fold extraction of prepared mixture with system chloroform - ethanol (2:1), concentrating extract, its chromatography on column with silica gel and the following separation of indole alkaloids by HPLC method. Method based on applying this strain provides enhancing yield of indole alkaloids. Invention can be used in preparing indole alkaloids eliciting an anti-tumor activity.
EFFECT: improved method for preparing, valuable medicinal properties of indole alkaloids.
4 cl, 1 tbl, 2 ex
FIELD: biotechnology, medicine, antibiotics.
SUBSTANCE: invention proposes to the new compound amycomycin of the molecular formula C65H115NO18 (structural formula is given on the invention claim) that shows an antibacterial activity. Amycomycin, its pharmaceutically acceptable salts and derivatives in all their stereoisomeris and tautomeric forms can be obtained by culturing microorganism Amycolatopsis sp. ST 101170 (DSM 12216) under aerobic conditions on the nutrient medium containing the necessary nutrient components. The end product is isolated and purified and converted if necessary to its pharmacologically acceptable salt, ester, ether and other chemical derivatives and eliciting the same spectrum of antibacterial activity. Amycomycin is a component of the pharmaceutical composition eliciting an antibacterial activity. Amycomycin acts as an antibiotic. Invention provides inhibition of microorganisms with resistance to vancomycin and teicoplanin used in treatment of infections caused by Staphylococcus aureus.
EFFECT: improved preparing method, valuable medicinal properties of amycomycin.
7 cl, 2 tbl, 4 ex
FIELD: biotechnology, in particular production of melanin which represents pigment of high antioxidant and anti-tumor activity.
SUBSTANCE: claimed method includes providing by selection of yeast strain Nadsoniella nigra which under deep cultivation accumulates up to 25 g/l of yeast biomass after 96 hours from cultivation starting. Melanin is isolated both from yeast biomass (intracellular melanin) and from cultural liquid (extracellular melanin). Intracellular melanin is isolated by extraction with alkali solution followed by melanin deposition with hydrochloric acid. To isolate extracellular melanin cultural liquid is treated with ethanol followed by melanin deposition with hydrochloric acid.
EFFECT: method if increased effectiveness.
FIELD: biotechnology, biochemistry, enzymes, microbiology.
SUBSTANCE: invention proposes a method for microbiological oxidation of N-, O- or S-heterocyclic mono- or multinuclear aromatic compounds. The method involves culturing the recombinant microorganism that expresses cytochrome P-450-dependent monooxygenase BM-3 from Bacillus megaterium with amino acid sequence represented in SEQ ID NO:2 that comprises at least one functional mutation in region 86-88 and, if necessary, at least one functional mutation in one of regions 73-82, 172-224, 39-43, 48-52, 67-170, 300-335 and 352-356. The prepared oxidation product is isolated from medium, Invention provides carrying out oxidation of organic compounds with enhanced degree of effectiveness.
EFFECT: enhanced effectiveness of enzymes.
17 cl, 1 tbl, 7 ex
FIELD: organic chemistry, biotechnology.
SUBSTANCE: invention refers to biotechnology and can be applied in food industry for sweeteners production. Method of indole-3-pyruvic acid production includes indole-3-pyruvic acid production with enzyme catalyzing transformation of tryptophane to indole-3-pyruvic acid and deaerating treatment or oxygen-removing treatment in acid conditions. And enzyme made of microorganism characterized with amino-acid oxidase activity and catalase activity is used. 4-(indole-3-ilmethyl)-4-hydroxy-2-oxoglutaric acid is made of indole-3-pyruvic acid and oxaloacetic acid and pyruvic acid. Methods are easy realized and enable to produce high yield products.
EFFECT: production of indole-3-pyruvic acid.
15 cl, 17 tbl, 4 ex
SUBSTANCE: invention relates to a method of producing (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid, which includes enantioselective enzymatic reduction of an aryl ketone in a reaction mixture containing oxidoreductase and carbamination of the obtained alcohol.
EFFECT: obtaining (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid.
19 cl, 1 tbl, 5 ex
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
EFFECT: valuable medicinal properties of strain.
5 cl, 8 dwg, 10 ex
FIELD: biotechnology, microbiology, agriculture.
SUBSTANCE: the strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
EFFECT: valuable properties of strain.
3 tbl, 2 ex
FIELD: biotechnology, agriculture, microbiology.
SUBSTANCE: invention relates to a new isolated strain of Lactobacillus acidophilus 1660/08 as a producer of the protein fodder. The strain Lactobacillus acidophilus 1660/08 is obtained by the selection method and selected by its ability to form significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.10.-DEP. Invention provides eliminating the environment pollution in producing the protein fodder, to enhance the specific protein yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process in its preparing, to simplify equipment fitting out and to utilize waste in manufactures using natural raw.
EFFECT: valuable properties of strain.
2 tbl, 10 ex