Producer antitumor melaninogenica drug "stromelysin"


(57) Abstract:

Usage: biotechnology, medicine, Microbiology, obtaining substances anticarcinogenic actions. The inventive application of the Antarctic strain 365 black yeast Nadsoniella nigra var.hesuelica as producer melaninogenica drug with high antitumor effect and does not exhibit toxicity.

The invention relates to biotechnology, in particular the production of biologically active substances of antitumor action.

Known microorganism producer melaninogenica substances belonging to Streptomyces melanogenes. However, the resulting melanin does not possess antitumor properties [1]

There are various drugs having anti-tumor activity, used in the treatment of cancer: benzoate, cyclophosphamide, adriablastin (lung cancer), fluorouracil (tumors of the colon), olivomycin, vincristine, prospidin (melanoma) and others [2] All of them are known to have harmful side effects, contraindications, and, in addition, require intravenous or intramuscular use.

Advantage or technical effect image the properties, not inherent in the known means of similar purpose, namely, the application of the known strain 365 Antarctic black yeast Nadsoniella nigra var. hesuelica as producer antitumor melaninogenica drug whose properties provide effective treatment of cancer.

Summary of the invention the use of this strain Antarctic black yeast Nadsoniella nigra var. hesuelica 365 as unlimited and legkovozvodimih source of melanin with antitumor properties and non-toxic, which makes its practical use is particularly valuable.

Strain 365 Antarctic black yeast Nadsoniella nigra var.hesuelica selected from the sample substrate Antarctic Islands Haswell and is known [3]

Produced by strain 365 black-brown pigment belonging to the group of melanin, is localized in the form of granules in the cell wall, making it quite dense outer layer of structurally and chemically closely related to other elements of the cell wall [4]

The process of selection of the target melaninogenica product from the biomass of cells of strain (and its main characteristics are described and include the destruction of the cells of the biomass, the Department piikoi resulting product is the presence in the structure of melanin strain 365 stable free radicals at a high concentration (1019/g) dry matter [6]

Get melaninogenicus drug called "Stromelysin". Discovered the useful properties of Astromania" lack of toxicity, antitumor effect were not previously known and is not described in the literature.

Usage data obtained from strain 365 Nadsoniella nigra var. heselica melaninogenica product for the treatment of volunteers with various forms of cancer are listed below.

Strain 365 cultivated by well-known methods in the conditions of aeration and mixing in liquid mineral medium of the following composition, g/l: NK2RHO40,1; l20,03; gSO40,05; NaCl, 0,05 with glucose (2%) and liquid yeast autolysate (1%) at 26-28oC and pH 3.5 during the week. The biomass was separated by centrifugation and repeatedly washed with distilled water, then biomass autoclave at 0.5 ATM with 0.5 n Paon within 3 hours of Cell mass is removed by centrifugation, centrifugal acidified with concentrated Hcl to pH 2-1. Sediment in the form of black flakes separated by centrifugation and repeatedly washed with distilled water. To obtain water-soluble drug residue dried and dissolved in a small amount of ammonia water (25% solution Ammi is Ernie crystals with bright shimmering Shine. Its physico-chemical properties correspond to the indicators listed in the above sources. The resulting powder was used to produce aqueous solutions of desired concentration of melanin.

Obtained from strain 365 drug showed high activity against human cancer cells, in particular cell line carcinoma of the colon (80%), cell lines of lung cancer (89%), as well as in the treatment of patients-volunteers:

Example 1. Patient Century, lung cancer, ingestion 40-10 mg daily dose, recovery 2 years.

Example 2. Patient I. (physician) melanoma, 1U stage, ingestion 60-10 mg daily dose, and locally to the lymph nodes, recovery 1 year 7 months old. high working capacity.

The drug is non-toxic (experiments on cell cultures and on the author C. P. Order). Side effects were observed.

Thus, from comparison with known means the same destination can be seen that the use of the drug melanin from strain 365 Nadsoniella nigra var.hesuelica avoids toxic side steps for effective mainly antitumor action. It follows that the strain Nadsoniella var. hesuelica 365 is a producer of high-performance protivoopujolevoe antitumor melaninogenica of the drug.


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