Method of regeneration of bone tissue in the experiment

 

(57) Abstract:

The invention relates to the field of treatment of various bone injuries, in particular cracks and fractures, and can be used in their treatment. With the aim of reducing the duration of the process of regeneration of bone tissue in the area of its damage or defect using a method in which before imposing a fixed bandage in place of damage or defect of the bone in the result of any injury (fractures, fracture and others) carry out the introduction of the solution containing the following ingredients (in g): 1-hydroxyethylidenediphosphonic acid 1,8-2,0; calcium chloride anhydrous 1,4-2,2; nitrate gadolinium, the uranyl 0.3 to 0.4; water to 1 l (pH of the solution set in the range of 7.5 to 8.0). The use of this method the process of regeneration of bone tissue at the site of injury is considerably accelerated and can be reduced in comparison with the traditionally used method of treatment of similar diseases in 5-8 times or more; correspondingly reduced and the recovery time of the normal physiological functions of the injured bone. While the above solution does not contain any toxic ingredients and does not cause the treatment process side of negati cracks and fractures, and can be implemented in medical and veterinary surgery.

There is a method of regeneration of bone tissue by fixing plaster cast [1] the Disadvantage of this known method is that the process of regeneration of bone tissue is happening here very slowly and restore normal physiological function of the injured part of the bone is delayed by a few weeks or even months, especially in elderly and senile age. Such a long involuntary restriction of mobility of the injured site of a bone having a negative impact on the surrounding muscle tissue, leading to negative side effects in the body.

There is also known a method of regeneration of bone tissue by fixing plaster cast with Windows in it at the level of fracture for installation of electrodes, applying a bandage soaked in a solution of calcium chloride, the flannel strip and impact on the location of the fracture electric current of 5 mA for 15 min, carried out for the entire period of treatment is 20 times [20] the Disadvantage of this technical solution, which is achieved technical effect and the totality of symptoms is the closest to the claimed object is to reduce the duration of regeneration of bone tissue at the site of damage or defect.

The declared objective is achieved by the fact that in the known method of regeneration of bone tissue by fixing plaster shell [2] in its damage or defect before applying plaster bandages carry out the introduction of a solution containing 1-hydroxyethylidenediphosphonic acid, calcium chloride, gadolinium nitrate and water in the following ratio of ingredients, wt.

1-Hydroxyethylidenediphosphonic acid, g 1,8 2,0

Calcium chloride anhydrous, g 1,4 2,2

Nitrate gadolinium, the uranyl, g 0,3 0,4

Water to 1 liter

pH 7,5 8,0

In use of the present invention, the process of regeneration of bone tissue, significantly accelerates time restore the normal physiological functions of the injured bone can be reduced compared with that for the prototype method [2] in 5 to 8 times and even more. Used to declare our object the solution does not cause any negative side effects, and in particular any significant necrosis adjacent to the bone muscle tissues.

In the literature so far has not been described any product or part, the introduction of which, in place of damage or defect of the bone tissue before applying plaster the Ani of the injured section of bone. This circumstance allows us to assert that the claimed object corresponds to the first criterial feature of the invention of "novelty". Comparison of the known characteristics of the prototype method [2] and characteristics make changes, namely the use of injection in place of damage or defect of the bone tissue of an aqueous solution containing 1-hydroxyethylidenediphosphonic acid, calcium chloride, gadolinium nitrate and an alkaline agent (required to bring the pH of the claimed our solution to the level of 7.5 to 8.0), it is not possible to predict that we observe in the experiment, a sharp reduction in the duration of recovery of normal physiological functions of the injured bone in the place of damage or defect compared with those for the known method [2] Therefore, there is every reason to believe, that claimed us technical solution is not obvious from the well-known in the art and consequently, it has the second criterion characteristic of the invention "inventive step". And finally, we state our method involves only one additional step compared with the method similar to [1] introduction (injection) solution, containing what can be accomplished even in the outpatient setting), and much easier to design compared with the method of the prototype [2] Therefore, we conclude that declare to us the object and satisfies the third criterion characteristic of the invention "industrial applicability".

Declare on the subject of the invention, the method of regeneration of bone tissue can be illustrated by the following examples.

P R I m e R 1

(preparation of the proposed solution)

In 950 ml of distilled water successively dissolve the above number 1-hydroxyethylidenediphosphonic acid, calcium chloride and nitrate gadolinium. After that, the resulting mixture was cautiously added dropwise concentrated alkali solution (for example, 10% aqueous sodium hydroxide solution) to achieve the necessary medium acidity (pH 7.5 to 8.0). In conclusion, the resulting composition is diluted with distilled water to a volume of 1000 ml. of the resulting solution can be immediately used for the implementation of the proposed method; if necessary, it can be a long time (not less than 1 year) is stored in a closed vessel without loss of physiological activity.

P R I m m e R 2

Prepare a solution of the following composition:

1-hydroxyethylidenediphosphonic,5

Thus prepared solution is injected under anesthesia domestic cat Nordic breed, which artificially made fracture tibia hind legs, 1 hour later after causing her injury, in the amount of 2.0 ml for 2 min in place of damaged bones. After injection of the fracture site of the bone conclude fixed in a plaster cast, in which the injured limb segments can stand up to the recovery of locomotor function. The process of regeneration of the injured bone tissue is controlled by the radiographic method.

Some characteristics of the process of regeneration of damaged bone tissue in this specific case is presented in table 1.

P R I m e R 3

Perform as example 2, but for injection use a solution of the following composition: 1-hydroxyethylidenediphosphonic acid, g 1,9

Calcium chloride anhydrous, g 1.7

Nitrate gadolinium, the uranyl, g 0,35

Water to 1 liter

pH 7.8

in the amount of 2.0 ml.

P R I m e R 4

Carried out according to the scheme of example 2, but for injection take the solution of the following composition:

1-hydroxyethylidenediphosphonic acid, g 2,0

Calcium chloride anhydrous, g 2,2< e R 5 (similar to [1])

Perform as example 2, but the solution indicated there composition in place of damage to the bone before applying fixed plaster bandages do not enter. Some characteristics of the process of regeneration of damaged bone tissue for this case are also presented in table 1.

P R I m e R 6 (prototype [2])

Perform as example 5, but in a cast cut out "window" at the level designated trauma to the bone for the electrodes to the skin of the animal at the injury site put a piece of bandage soaked in 5% aqueous solution of calcium chloride, then the flannel strip and the active "+" electrode on the opposite surface of the indifferent electrode ("-") gasket, moistened with water, then attach the electrodes to the electroplating apparatus "Thread-1" and ignore the current 5 mA for 15 minutes, the procedure Described above (the so-called "electrophoresis") during bone regeneration periodically repeated (the number of such sessions for the entire course of treatment 20).

Some characteristics of the process of regeneration of damaged bone tissue for this case are also presented in table 1.

P R I m e R 7

Prepare a solution of the composition indicated in example 2, and then enter it under narkotoi front paw with a significant offset, after 6 hours after application to her injuries. The drug is administered in the amount of 2.0 ml for 2 min, after which travmirovannomu the sign in plaster shell. The process of bone regeneration control radiographic method.

Some characteristics of the process of regeneration of damaged bone tissue for this case are shown in table 1.

P R I m e R 8

Carried out using the General scheme of example 7, but for injection use solution, the composition of which is specified in example 3, in the amount of 2.0 ml.

P R I m e R 9

Carried out as example 7, but for injection use solution, the composition of which is given in example 4, in the amount of 2.0 ml.

P R I m e R 10 (similar to [1])

Perform under the scheme of example 7, but the solution indicated there composition before applying plaster bandages in travmirovannomu place the bone tissue does not enter.

P R I m e R 11 (prototype [2])

Perform under the scheme of example 6, but for fracture treatment, the nature of which is specified in example 7.

P R I m e R 12 (comparative)

Perform, using the General scheme of example 7, but for injection use solution of the following composition:

1-hydroxyethylidenediphosphonic acid, g 1,5

Calcium chloride is="ptx2">

P R I m e p 13 (comparative)

Done using the same scheme as in example 7, but for injection use a solution of the following composition:

1-hydroxyethylidenediphosphonic acid, g 2,2

Calcium chloride anhydrous, g 2,5

Nitrate gadolinium, the uranyl, g 0,5

Water to 1 liter

pH 8,2

in the amount of 2.0 ml.

P R I m e R 14

Prepare a solution of the composition indicated in example 3, and then put him under anesthesia dog (French bulldog), which was hit by a moving car and taken to the hospital with a diagnosis of closed fracture of both femurs" later, 3 days after injury, in the amount of 2.5 ml for 2 min in the corresponding damaged area. Then the damaged area is enclosed in a plaster cast. The process of bone regeneration control radiographic method.

Some characteristics of the process of regeneration of bone tissue for the specified cases are presented in table 1.

P R I m e R 15

Prepare a solution of the composition shown in example 3, and then put him under anesthesia dog (breed German shepherd), which was brought to the clinic with a natural way to the trauma (diagnosis: closed fracture p the solution is injected in the amount of 2.5 ml for 2 min and then enter into the damaged area of bone in a fixed plaster shell. The process of bone regeneration control radiographic method.

Some characteristics of the process of regeneration of bone tissue for this case are also presented in table 1.

P R I m e R 16

Prepare a solution of the composition indicated in example 2, and then enter it anesthetized rabbit, which artificially cause deliberate injury of both femur by drilling under General anesthesia with an electric drill with a drill diameter 5 mm, While the introduction of the above solution in the amount of 1.0 ml (time 2 min) is carried out in travmirovannomu only one of the injured bone (the other left as a control). After that all the injured place conclude fixed in a plaster cast. Control over the process of regeneration of bone tissue perform radiographic method.

Some characteristics of the process of regeneration of bone tissue for this case are shown in table 1.

P R I m e R 17

Carried out as described in example 16 scheme, but for injection use solution, the composition of which is specified in example 3, in the amount of 1.0 ml.

P R I m e R 18

Perform as example 16, n is 9 (comparative)

Perform according to the General scheme of example 16, but for injection use solution, the composition of which is specified in example 12, in the amount of 1.0 ml.

P R I m e R 20 (comparative)

Spend as example 16, but for injection use solution, the composition of which is given in example 13, in the amount of 1.0 ml.

P R I m e R 21 (similar to [1])

Carried out as example 16, but the solution indicated there into the injury site of any of injured bones do not enter.

Some characteristics of the process of regeneration of bone tissue for the marked case is given in table 1.

P R I m e R 22

Perform under the scheme of example 6, but for the treatment of damaged bone, the nature of which is specified in example 16.

Some characteristics of the process of regeneration of bone tissue for the marked case is also shown in table 1.

Of the produced data in table 1 clearly shows that the use of the claimed our method compared with the method of the prototype [2] significantly reduce the duration of regeneration of bone tissue at the site of injury, and this phenomenon is observed regardless of the type subjected to injury of animals. At the same time the trail is Vlada significant and reduce their quantities below the bottom of the proposed limits is accompanied by an increase in the duration of the process of regeneration of bone tissue, exceeding the upper declare does not give any appreciable reduction in the duration of the regeneration, but is accompanied unproductive expenditure included in this solution ingredients. Note also that we conducted to measure the strength of the regenerated using the method of [2] and using the claimed our way bone tissues showed no appreciable differences between them. And finally, we state our solution, according to the clinical picture of blood regeneration of the injured bone, and observations on the subjective condition of injured animals in the course of their treatment (appetite, reactivity, etc), does not have any pronounced toxicity; in addition, each of the members of this solution ingredients, according to [3-4] also not is a toxic compound.

Method of regeneration of bone tissue in the experiment by fixing the fragments of plaster cast with the subsequent announcement in the fracture area of calcium chloride, characterized in that the fracture area, introduce a solution containing 1-hydroxyethylidenediphosphonic acid, calcium chloride, gadolinium nitrate, the uranyl and water in the following, zootoxin the 1,4 2,2

Nitrate gadolinium, the uranyl 0,3 0,4

Water Up to 1000

pH 7.5 to 8.0

 

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