A method of obtaining a biomaterial for use in ophthalmology
(57) Abstract:The proposed method can be used to prepare materials in surgical treatment of myopia of high to medium, to obtain scleroplastic material and antiglaucoma drains. How is that as an animal feedstock use pericard, which is mechanically peeled, cut, five frozen and thawed in 1% solution of sodium chloride and distilled water in the ratio 1 : 1 from 48 to 72 hours The invention relates to medicine, and more specifically to ophthalmology, and can be used to prepare materials in surgical treatment of myopia of high to medium to obtain scleroplastic material in surgical treatment of glaucoma for receiving antiglaucoma drainage, as well as a source of solutions of collagen in the treatment of myopia, mild and manufacturing exterior dosage forms.There is a method for the treatment of myopia, which is implemented through the preparation and use of material from the tissue of the pericardium. The material is poured saline with antibiotics for 1-2 h, and then treated with an aqueous solution AMI of this method is that, what treatment does not lead to the destruction of stromal cells of the pericardium, blood vessels are not reported other unrelated Vysokomolekulyarnye biological substance, and an aqueous solution of brilliant green has a relatively weak antiseptic properties.The aim of the invention is to obtain a biomaterial for use in ophthalmology, where the source is animal raw materials use the pericardium of a bull, which is appropriately cleaned and treated with solutions.The technical result that is achievable with the use of the invention is to obtain a versatile biomaterial, which can be widely used for some products, such as material for scleroplasty, antiglaucoma drainages, the material for the treatment of macular dystrophies and to obtain a liquid formulation.The technical result is achieved that is used as raw material pericardium of a bull, which is mechanically peeled, cut, five frozen and thawed in 0.9% sodium chloride solution and distilled water in the ratio 1:1, twice placed in a 1% solution of ammonia and ethanol in the ratio 1: 1 from 48 to 72 h, parilized-irradiation dose of 2.5 Mgrad.The pericardium is the connecting membrane of the heart, contains few blood vessels, cells, stroma it consists mainly of collagen, which is poorly soluble, has a high mechanical strength and is nishanthini. In addition, the solution of this collagen has a high enzymatic stability, which is very important in the manufacture collagenolytic materials.Mechanically cleaned pericardium is cut to the desired dimensions, subjected to five freeze and thawing in a solution of sodium chloride and distilled water to exit the tissue Vysokomolekulyarnye proteins and glycoproteins, which, as you know, are the main antigens of this fabric. The experiment showed that for their full output from this tissue sufficiently five times freezing and thawing.To release the tissue from lipids, lipoproteins fabric treated with 1% solution of alcohol and ammonia from 48 to 72 hours, This procedure is repeated twice. Experimentally it was found that incubation of slices of the pericardium should be conducted in such a solution is not less than 48 hours, because with less time sufficient output lipids occurs. Incubation for 72 h rises is the project of aqueous ammonia and alcohol pieces of tissue are placed in distilled water. It is established that the release of ammonia and alcohol (mainly ammonia) occurs after 5 times in 15 minutes, with less time washing noted traces of ammonia and acidic pH values. 30 minute cleaning completely removes the ammonia from the tissue and therefore more time for cleaning is not required.Preserving and simultaneously sterilizing medium for pieces is 70% ethyl alcohol, and the standard sterilization irradiation.The method is as follows.The pericardium mechanically cleaned of fat, blood vessels and other contaminants.Cut into pieces of desired size and configuration. Rinse in tap water.Prepare a solution of sodium chloride (0.9% ) and distilled water 1:1 and fill them with pieces of cloth, put them in the freezer and thawed after freezing. The procedure is repeated 5 times.Prepare 1% solution of ammonia and 1% solution of ethyl alcohol, mixing them in the ratio of 1:1 and pour this solution on the pieces.After 48-72 h, the solution is drained and replaced with fresh.After the second incubation, the solution is drained, and the pieces are washed five times for 15-30 min in distilled water, changing water every time n is m ethanol and sterilized by irradiation dose of 2.5 Mgrad.Examples of specific implementation method.P R I m e R 1. Receiving antiglaucoma drains.Well purified stroma pericardium cut into strips with a width of 0.2 cm and five were frozen and thawed in 0.9% sodium chloride solution and distilled water, then washed and placed the strips in a solution of ammonia (1%) and ethyl alcohol (1%), were incubated in this solution for 48 h twice, and then washed five times in distilled water for 15 min at pH of 6.7-7.0 laundering stopped and rolled strips in vials with 70% ethyl alcohol. Sterilized by irradiation and gave after bacteriological monitoring in the clinic.P R I m m e R 2. Preparation scleroplastic material.Well purified stroma pericardium was cut down to standard cutting, five were frozen and thawed in 0.9% sodium chloride solution and distilled water, double-incubated slices for 50 h in a solution of ammonia and alcohol (1%), then washed pieces in distilled water five times for 20 min at pH 6.5-7.0 and at the negative reaction to the ammonia washing was stopped, was packaged pieces in vials with 70% ethanol, sterilized by irradiation and passed to the clinic.
ezali slices of the required size, five were frozen and thawed as in the previous case, laundered and 72 h were incubated slices in a solution of ammonia and ethyl alcohol (1%), washed five times for 30 min and at pH 6.5-7.0 laundering stopped. Packaged in vials with 70% ethanol, sterilized by irradiation and passed to the clinic.A method of OBTAINING a BIOMATERIAL FOR USE IN OPHTHALMOLOGY, including the processing of pericardium farm animals solutions, characterized in that is used as raw material pericardium of a bull, which is mechanically peeled, cut, five frozen and thawed in 0.9% sodium chloride solution and distilled water in the ratio 1 : 1, twice placed in a 1% solution of ammonia and ethanol in the ratio 1 : 1 from 48 to 72 h, and then washed five times for 15 - 30 min in distilled water, placed in a solution of 70%aqueous ethanol and sterilized by irradiation dose of 2.5 Mgrad.
SUBSTANCE: method involves applying surgical intervention and antitumor therapy. After having removed a part or the whole lung, mediastinum is opened by cutting out horseshoe-shaped or rectangular flap of mediastinal pleura and carrying out lymphodissection. Then, hemostatic sponge is placed in mediastinum. The sponge is impregnated with antitumor chemotherapeutical preparations in intraoperative mode. Pleura is sutured above the sponge. Another hemostatic sponge impregnated with cytostatic preparations in intraoperative mode is attached to visceral pleura of interlobular sulcus.
EFFECT: prolonged chemotherapeutical preparations delivery to malignant neoplasm foci or subclinical metastases.
SUBSTANCE: the suggested multi-purpose heterogeneous collagen matrix for implantation is being flexible-elastic mass obtained out of two collagen sources, moreover, one source is a tissue of vertebrates of one and the same class, and another source - that of an animal of another class, moreover, collagen sources are animal tissues of, for example, mammalian class and avian class, matrix consists of two phases: solid phase - as microspheres out of mammalian tissue collagen, and liquid phase - out of denaturated avian tissue collagen at the ratio of phases being (1-10) : (1-10), at microspheres size being 100-300 mcm, as for final products of biodegradation they are represented by CO2 and H2O. Matrix, additionally, contains components of physiological culture media, and, also, additives that favor the growth and differentiation of cells and tissues, antibacterial and/or antiviral components, and, also, antiaggregation preparations in their efficient quantity, for example, additionally, it contains embryonic cells of nervous tissue. Another aspect of the present innovation deals with the method to obtain the matrix due to preparing mammalian collagen solution (MCS) and denaturated avian collagen solution (ACS), moreover, it is necessary to apply 0.3 M acetic acid, at final concentration for MCS being approximately 0.5-1.5%, and for ACS - approximately 3.0-5.0%, then MCS should be treated with γ -irradiation at the dosage of 1.0 Mrad to be further homogenized to obtain microspheres. Then both MCS and ACS should be washed off with distilled water up to pH of not less than 6.0 and with phosphate buffer solution to mix washed off mammalian collagen and avian collagen at 1:1 ratio at obtaining matrix. The matrix obtained should be additionally supplemented with antibacterial and/or antiviral components, and, also, stimulating agents for tissue regeneration and antiaggregation preparations. The matrix obtained should be sterilized due to γ -irradiation at the dosage of 0.5 Mrad/1 ml. The present innovation enables to obtain new heterogeneous collagen matrix which is considered to be a multi-purpose one applied for transplantology and substitution surgery of different tissues and organs in alive body in case of tissue lesions. Moreover, it is distinguished by controlled terms of biodegradation.
EFFECT: higher efficiency of application.
13 cl, 16 ex, 4 tbl
FIELD: medicine, traumatology.
SUBSTANCE: osseous defect after filling with collapan and usage of metallofixative should be covered with absorbable collagen film that provides orientation of osseous regenerate's organization.
EFFECT: higher efficiency of therapy.
FIELD: peptides, pharmacy.
SUBSTANCE: invention relates to low-molecular derivatives of peptides that are able to act as inhibitors in interaction between laminine and nidogen (interactions laminine/nidogen). Also, invention relates to a method for their preparing, pharmaceutical composition prepared on thereof and their using for preparing pharmaceutical agents, and for identification of inhibitors in interaction laminine/nidogen.
EFFECT: valuable properties of peptides.
5 cl, 12 dwg
FIELD: medicine, experimental medicine.
SUBSTANCE: one should introduce tripeptide Pro-Gly-Pro for laboratory animals as injections at the quantity of 0.09-1.0 mg/kg body weight, and, also, gelatin as fodder additive. The method suggested enables to suppress appetite, decrease the quantity of fodder intake that leads to decreased body weight as a result.
EFFECT: higher efficiency.
2 cl, 5 dwg, 5 ex
FIELD: medicine, dermatology, in particular treatment of trophic ulcer (TU) and long-term open septic wound (LTOSW).
SUBSTANCE: claimed method includes application of wound-healthing composition onto TU and LTOSW in the first step of wound process. Said composition contains (mass %): methyluracil 0.9-1.1; pepsin 3.4-4.4; bentaine hydrochloride 3.6-4.4; sodium chloride 9.0-11.0; and balance up to 100,0: distilled water. In the second and third phases fine cut collagen preparations in form of 3-4 mm thickness layer are applied on purified TU and LTOSW surface. Then cotton carrier freely moistened with wound-healthing composition diluted with water in ratio of 1:2 is layered onto collagen layer. Multihole microirrigator and further sterile cotton carrier are sequentially applied over abovementioned cotton carrier.
EFFECT: effective treatment due to accelerated abruption of necrotic tissues, inhibition of microbial growth, decreased risk of infective, toxic and allergic affects, and improved tissue regeneration.
2 ex, 3 cl
SUBSTANCE: the present innovation deals with the method to accelerate mucosal healing due to the following technique: one should apply a membrane consisted of purified collagenic material obtained out of natural collagen-containing tissue onto the part of affected mucosa to provide the chance for mucosal reconstruction in this part and, also, it deals with mucosa-regenerating preparation and application of purified collagenic material obtained out of collagen-containing natural tissue for preparing mucosa-regenerating preparation. The innovation provides more modified method that accelerates mucosal regeneration, as a whole, and, particularly, after surgical operations associated with the plasty of oral fornix.
EFFECT: higher efficiency.
12 cl, 3 dwg, 5 ex
FIELD: chemical engineering.
SUBSTANCE: method involves pretreating bone tissue of stock-farm animals, comminuting it, precipitating the end product and drying it by lyophilizing. The bone tissue is degreased with alcohol-ether mixture. Demineralization 0.5 and HCl is carried out after grinding during 20 h. Non-resolvable matrix is subjected to proteolysis in HCl of pH=2.0 in pepsin presence at 37°C during 18 h. Then it is centrifuged at 40000g. The end product is precipitated from supernatant with ammonium sulfate to 25% saturation and centrifuged at 6000g. The precipitate is lyophilized against distilled water and chromatographically purified using CM-Sephadex at pH=4.8 and dried with lyophilization.
EFFECT: low cost collagen production; high purity cosmetic preparation production.
FIELD: medicine, endocrinology, pharmacy.
SUBSTANCE: invention relates to a pharmaceutical composition comprising epidermal growth factor (EGF) used in treatment of wounds on skin and soft tissues of lower limb in diabetic patient. Method of treatment involves topical infiltration of EGF-containing solution into different points and by contours of tissue damaged zone to provide administration of the solution into wound in the total volume 4-20 ml and irrigation of all deep surface of wound base and edges with the indicated composition. Invention provides prevention of diabetic limb amputation, stimulation of cellular proliferation in patients with foot ulcer being especially in geriatrics.
EFFECT: valuable medicinal properties of pharmaceutical composition.
19 cl, 1 tbl, 9 ex
SUBSTANCE: method involves carrying out mechanical treatment of biological connective tissue and following treatment with solutions. Animal or human connective tissue is hold in solution of collagen and/or its derivatives and/or collagen processing products at 25-65°C and exposed to ionizing radiation treatment.
EFFECT: improved biocompatibility and physicochemical properties; prolonged drug action.