A method of obtaining a biomaterial for use in ophthalmology

 

(57) Abstract:

The proposed method can be used to prepare materials in surgical treatment of myopia of high to medium, to obtain scleroplastic material and antiglaucoma drains. How is that as an animal feedstock use pericard, which is mechanically peeled, cut, five frozen and thawed in 1% solution of sodium chloride and distilled water in the ratio 1 : 1 from 48 to 72 hours

The invention relates to medicine, and more specifically to ophthalmology, and can be used to prepare materials in surgical treatment of myopia of high to medium to obtain scleroplastic material in surgical treatment of glaucoma for receiving antiglaucoma drainage, as well as a source of solutions of collagen in the treatment of myopia, mild and manufacturing exterior dosage forms.

There is a method for the treatment of myopia, which is implemented through the preparation and use of material from the tissue of the pericardium. The material is poured saline with antibiotics for 1-2 h, and then treated with an aqueous solution AMI of this method is that, what treatment does not lead to the destruction of stromal cells of the pericardium, blood vessels are not reported other unrelated Vysokomolekulyarnye biological substance, and an aqueous solution of brilliant green has a relatively weak antiseptic properties.

The aim of the invention is to obtain a biomaterial for use in ophthalmology, where the source is animal raw materials use the pericardium of a bull, which is appropriately cleaned and treated with solutions.

The technical result that is achievable with the use of the invention is to obtain a versatile biomaterial, which can be widely used for some products, such as material for scleroplasty, antiglaucoma drainages, the material for the treatment of macular dystrophies and to obtain a liquid formulation.

The technical result is achieved that is used as raw material pericardium of a bull, which is mechanically peeled, cut, five frozen and thawed in 0.9% sodium chloride solution and distilled water in the ratio 1:1, twice placed in a 1% solution of ammonia and ethanol in the ratio 1: 1 from 48 to 72 h, parilized-irradiation dose of 2.5 Mgrad.

The pericardium is the connecting membrane of the heart, contains few blood vessels, cells, stroma it consists mainly of collagen, which is poorly soluble, has a high mechanical strength and is nishanthini. In addition, the solution of this collagen has a high enzymatic stability, which is very important in the manufacture collagenolytic materials.

Mechanically cleaned pericardium is cut to the desired dimensions, subjected to five freeze and thawing in a solution of sodium chloride and distilled water to exit the tissue Vysokomolekulyarnye proteins and glycoproteins, which, as you know, are the main antigens of this fabric. The experiment showed that for their full output from this tissue sufficiently five times freezing and thawing.

To release the tissue from lipids, lipoproteins fabric treated with 1% solution of alcohol and ammonia from 48 to 72 hours, This procedure is repeated twice. Experimentally it was found that incubation of slices of the pericardium should be conducted in such a solution is not less than 48 hours, because with less time sufficient output lipids occurs. Incubation for 72 h rises is the project of aqueous ammonia and alcohol pieces of tissue are placed in distilled water. It is established that the release of ammonia and alcohol (mainly ammonia) occurs after 5 times in 15 minutes, with less time washing noted traces of ammonia and acidic pH values. 30 minute cleaning completely removes the ammonia from the tissue and therefore more time for cleaning is not required.

Preserving and simultaneously sterilizing medium for pieces is 70% ethyl alcohol, and the standard sterilization irradiation.

The method is as follows.

The pericardium mechanically cleaned of fat, blood vessels and other contaminants.

Cut into pieces of desired size and configuration. Rinse in tap water.

Prepare a solution of sodium chloride (0.9% ) and distilled water 1:1 and fill them with pieces of cloth, put them in the freezer and thawed after freezing. The procedure is repeated 5 times.

Prepare 1% solution of ammonia and 1% solution of ethyl alcohol, mixing them in the ratio of 1:1 and pour this solution on the pieces.

After 48-72 h, the solution is drained and replaced with fresh.

After the second incubation, the solution is drained, and the pieces are washed five times for 15-30 min in distilled water, changing water every time n is m ethanol and sterilized by irradiation dose of 2.5 Mgrad.

Examples of specific implementation method.

P R I m e R 1. Receiving antiglaucoma drains.

Well purified stroma pericardium cut into strips with a width of 0.2 cm and five were frozen and thawed in 0.9% sodium chloride solution and distilled water, then washed and placed the strips in a solution of ammonia (1%) and ethyl alcohol (1%), were incubated in this solution for 48 h twice, and then washed five times in distilled water for 15 min at pH of 6.7-7.0 laundering stopped and rolled strips in vials with 70% ethyl alcohol. Sterilized by irradiation and gave after bacteriological monitoring in the clinic.

P R I m m e R 2. Preparation scleroplastic material.

Well purified stroma pericardium was cut down to standard cutting, five were frozen and thawed in 0.9% sodium chloride solution and distilled water, double-incubated slices for 50 h in a solution of ammonia and alcohol (1%), then washed pieces in distilled water five times for 20 min at pH 6.5-7.0 and at the negative reaction to the ammonia washing was stopped, was packaged pieces in vials with 70% ethanol, sterilized by irradiation and passed to the clinic.

ezali slices of the required size, five were frozen and thawed as in the previous case, laundered and 72 h were incubated slices in a solution of ammonia and ethyl alcohol (1%), washed five times for 30 min and at pH 6.5-7.0 laundering stopped. Packaged in vials with 70% ethanol, sterilized by irradiation and passed to the clinic.

A method of OBTAINING a BIOMATERIAL FOR USE IN OPHTHALMOLOGY, including the processing of pericardium farm animals solutions, characterized in that is used as raw material pericardium of a bull, which is mechanically peeled, cut, five frozen and thawed in 0.9% sodium chloride solution and distilled water in the ratio 1 : 1, twice placed in a 1% solution of ammonia and ethanol in the ratio 1 : 1 from 48 to 72 h, and then washed five times for 15 - 30 min in distilled water, placed in a solution of 70%aqueous ethanol and sterilized by irradiation dose of 2.5 Mgrad.

 

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