Installation for freeze-drying of biomaterials

 

(57) Abstract:

Usage: for regulating the temperature of the dried material in the technique of freeze drying. The inventive installation for freeze-drying contains an absorption chamber, a cooling chamber, the collector-vigorazhival in the form of a tube with fittings for connection vials of dried material and flange for placement on the neck of the Dewar vessel and the vacuum system piping. The plant is equipped with a draining pipe with electric heating element and the control unit with temperature sensor. Item is located in the lower part of the vent tube. The venting tube is placed with a gap in the tube collector-memoratives. The electric heating element is connected with a control unit located inside of the vent tube below the level of nitrogen. The absorption chamber is in the form of a hollow cylinder with a lid. The cylinder is fixed on a part of the collector-memoratives. The last is placed in the cavity of the Dewar vessel and made a hole. The sensor is connected to the control unit and is installed in a cooling chamber connected by pipeline with a draining pipe . In the hollow cylinder installation the second control unit, connected to the sensor that is installed in the cooling chamber. 1 C.p. f-crystals, 2 Il.

The invention relates to the technique of freeze-drying and can be used in microbiological and other industries for drying of cultures of microorganisms.

The known device [1] [2] for the drying of biomaterials in vacuum at low temperatures, containing a cylinder with silica gel, placed in a Dewar flask, the neck of which it is drying chamber. Inside it is a cooling chamber, in which the capsules with dried material. In these devices it is necessary to make the regeneration of the adsorbent after each drying cycle. In addition there is no possibility of regulating the temperature of the material during freezing and drying.

Known also [3] for freeze-drying, containing adsorption chamber made in the form of a sealed cylinder to a side which is attached a tubular manifold which in turn are attached combs with capsules containing dried material and the pipeline connecting the system with a vacuum pump. Under collecto aleemi of the material in the drying process. The temperature of the material in the drying process is regulated manually by adding dry ice in the cooling chamber.

The disadvantage of this setup is the need for regeneration of gypsum after each drying cycle and the impossibility of automatically regulating the temperature of the material during drying.

The task solved by the proposed installation is to regulate the temperature of the dried material and capacitor-memoratives in automatic mode, as well as simplified maintenance installation.

To solve this problem, the apparatus for freeze-drying of biomaterials containing an adsorption chamber, the cooling chamber, the collector-you - marival made in the form of a tube with fittings for connection vials of dried material and flange for placement on the neck of the Dewar vessel, and a vacuum system piping inside the collector-memoratives placed with a gap vent tube with the electric heating element placed in its lower part, and a control unit with temperature sensor and heating element is located inside the vent tube below the level of nitrogen and connected to bloccati collector memoratives, made perforated. The sensor is connected to the control unit and is installed in a cooling chamber connected by pipeline with a draining pipe.

For the separate regulation of the temperature in the collector-wykorzystane and a cooling chamber in the proposed apparatus, in addition, in the hollow cylinder installed additional venting tube with electric heating element in the bottom part connected with the second control unit, connected in turn with the sensor installed in the cooling chamber. The first control unit connected with the sensor installed in the exhaust pipe, placed in the collector-wykorzystane.

In Fig. 1 presents the proposed installation, General view; Fig. 2 installation additional venting tube, a General view.

Installation of freeze drying (Fig. 1) contains the collector-vigorazhival made in the form of a tube 1, is provided in the collector part of the fittings 2 for connection of 3 vials with the biomaterial, and the submersible part of the flange 4, is installed through the gasket 5 on the neck of the Dewar vessel 6. Inside the tube 1 along its entire length with a gap set exhaust pipe 7. The immersion cha is the form of a hollow closed at the top and bottom of the cylinder 8. In the cover 9 of the cylinder made a hole in which is inserted a pipe 10, a connecting pipe 11 with the vacuum pump 12.

Part of the tube 1 located in the hollow cylinder 8 made of perforated. In the lower part of the exhaust tube 7 below the level of nitrogen in the Dewar vessel 6 posted by electric heating element 13 is connected by a wire 14 passing inside the exhaust tube 7, with the control unit 15.

In the cooling chamber 16 located under the collector part of the tube 1, has thermosensor 17 connected to the control unit 15. The exhaust pipe 7 is connected to the cooling chamber 16 18 pipe.

The installation shown in Fig. 2, provided additional venting tube 19 mounted in the hollow cylinder 8. In the lower part of the vent tube 19 has an electrical heating element 20 connected to the control unit 21. In the cooling chamber 16 has a temperature sensor 22 connected to the control unit 21.

The exhaust pipe 19 is connected to the cooling chamber 16. The sensor 17 is installed in the exhaust pipe 7, the output of which is open. The sensor 17 is connected to the control unit 15.

Installation (Fig. 1) works as follows.

Ampoules with 3 dried material connected to the socket 2. The exhaust pipe 7 is connected by a pipe 18 with the cooling chamber 16 mounted under the manifold part of the tube 1 so that the ampoule 3 is located in the chamber 16 of the cooling.

The control unit 15 sets the temperature of the freezing chamber 16. To achieve the desired temperature, the control unit 15 includes an electrical heating element 13. Nitrogen boils in the exhaust pipe 7, which is formed the pair arrive in the cooling chamber 16. When this cooled ampoule 3 and the surface of the exhaust tube 7, which is wykorzystaniem.

After reaching the set temperature in the cooling chamber 16 unit 15 turns off the heating element. Boiling of nitrogen ceases. When the temperature in the chamber 16, the cycle repeats.

After freeze turns on the vacuum pump 12, the valve on the pipe 11 opens, begins the drying process.

After drying ampoules with a dry material sealed.

In the installation (Fig. 2) on the block 15 is to control the temperature in the cooling chamber 16. When this is done independent control of the temperature in the cooling chamber and the collector-wykorzystane.

In comparison with known devices proposed installation is possible to regulate the temperature of the collector-WiMo - Raiatea and cooling chamber in a wide range (from room temperature to minus 190aboutC) and automatically maintain the desired temperature with high accuracy.

The location of the vent tube inside the reservoir to attach vials as possible to reduce the distance between the surfaces of sublimation and desublimation, reducing drying time.

Placing the electric heating element inside the vent tube allows the selection of nitrogen vapors for cooling the outer chamber without creating excessive pressure in the Dewar vessel, which increases the safety of operation and simplify installation.

1. INSTALLATION FOR FREEZE-DRYING of BIOMATERIALS containing an adsorption chamber, the cooling chamber, the collector-vigorazhival in the form of a tube with stutzeri to connect vials of dried material and flange for placement on the mountains of the ne tube with an electric heating element, placed in its lower part, and a control unit with temperature sensor and the exhaust tube is placed with a gap in the tube collector-memoratives, the electric heating element is connected with a control unit located inside of the vent tube below the level of nitrogen, and the absorption chamber is in the form of a hollow cylinder with the lid sealed on the part of the collector-memoratives which is placed in the cavity of the Dewar vessel and made perforated, the sensor connected to the control unit and is installed in a cooling chamber connected by pipeline with a draining pipe.

2. Installation under item 1, characterized in that the hollow cylinder installed additional venting tube with electric heating element connected to the second control unit connected to the sensor that is installed in a cooling chamber connected to the additional gas outlet tube, and a first control unit connected to the temperature sensor and the electric heating element mounted in the exhaust tube located in the tube collector-memoratives.

 

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