The method of obtaining crystalline lactulose patterns
(57) Abstract:Usage: as a medicinal product for the treatment of liver encefalopatia and dietary treatment of newborns. The inventive lactulose crystal structure, the water content of 0.8%. Reagent 1: syrup of lactulose with national concentration was 65.3%. Reaction conditions: evaporation under reduced pressure of less than 0.2 bar, the temperature is below 60°C to a concentration of about 85 wt.% with subsequent sowing of crystalline lactulose and continued evaporation at 65 - 75°C. for 3 h.p. f-crystals. The invention relates to methods for lactulose crystal structure that does not contain water in crystals.Lactulose is a carbon, which can be obtained by isomerization of lactose, which is usually carried out under alkaline conditions in water solutions.It has been proven that the connection pharmaceutically applicable due to its regular effect on the intestinal bacterial flora, in particular in the treatment of hepatic encephalopathy and dietary treatment of newborns.Lactulose is prepared in the form of a syrup, which after purification can be administered to the patient. It would be however, it is more convenient floor is Oia, requirements for getting so dry lactulose, are stability, high purity, lack of hygroscopicity.It was cooked a few dry forms of lactulose.They can be classified according to the following main types:
lactulose on solid media; suitable solid carriers for this purpose are vegetable fibers, which are coated with the syrup of lactulose (for example, by spray drying), or an adsorbent, such as diatomaceous earth, absorbent kaolin, activated lignin, synthetic resin-adsorbent or activated charcoal;
amorphous lactulose, which can be obtained by rapid or slow cooling of a concentrated syrup of lactulose, by drying the syrup spray, by caramelization syrup or by drying of lactulose in the form of foam;
crystalline lactulose, which is usually obtained by crystallization of the sugar from the alcohol solution. A serious disadvantage of this type of solid lactulose is the presence of an organic solvent (usually methanol) in the crystals.Lactulose is also possible to crystallize from aqueous solution.According to the publication of Japanese patent N the ri 60-110aboutWith the subsequent addition of lactulose crystals when the two are thoroughly mixed to obtain a final concentration of 94-98% and keeping the mixture at 35-70aboutWith as much time as required for complete solidification of lactulose, usually for about 12-18 hours With the aim of obtaining crystalotherapy powder, solid lactulose is required to break during milling operations.In the published European patent N 0348630 described a very long way that lactulose is crystallized at 5-40aboutC. in Addition to the duration of the procedure (up to 60 h) another disadvantage is the low yield of crystalline product (less than 70% of lactulose present in the solution, crystallizes in fact).The invention provides a quick way of complete metamorphosis lactulose present in the aqueous solution or suspension, crystalline powder during a single stage of obtaining.According to the present invention the method is characterized by simultaneous continuous mixing by reducing the water content by evaporation of an aqueous solution or suspension of lactulose, possibly in the presence of seeds of crystals, to obtain free flowing TEI water. Seeding crystals that can be used according to the invention preferably consist of crystalline lactulose, containing no water.The original solution may have a low concentration of lactulose, which rises during the process of reducing the water content by evaporation and continued stirring. This reduction of the water content by evaporation preferably takes place under reduced pressure, in particular at a pressure of less than 200 mbar, particularly when lower than 100 mbar. The content of lactulose on the basis of total dry matter may preferably be not less than 80% and more in particular may be at least 95%
The possible addition of seed crystals must occur when the concentration and temperature, when no significant dissolution of the crystals, preferably at a content of dry substance in the solution 80-95% If the initial solution is already satisfy this last requirement, the seed crystals may be present from the very beginning.The number of added seed crystals depends on the temperature of the product, and also correlated with the dry matter content in the solution of lactulose.It is advisable, h is predpochtitelno 1-5% while reducing the water content by evaporation the temperature of the product is necessary to keep below the temperature of decomposition of lactulose, and preferably it should not exceed 70-80aboutC.During the process, the viscosity of the material increases extremely significantly, especially at the beginning of the crystallization process. So you need a very powerful mixing equipment.The decrease in the content by evaporation and simultaneous mixing continued until until all of the material will not be turned into a powder, and preferably to a water content below 1 wt.Powder lactulose with the crystal structure obtained according to the invention, can be used for pharmaceutical purposes in the form of a pharmaceutical composition or in the form of a food product, either for people or for use in veterinary medicine.P R I m m e R. Crystallization of lactulose.50 kg of lactulose syrup with an initial concentration of lactulose in 65,3% by mass was evaporated in the drying equipment type ZA100 (R;p;ker) under reduced pressure of less than 0.2 bar (absolute) at a temperature below 60aboutWith concentrations up to about 85 wt. what took 100 minutesIn the thus obtained solution was seeded 2.4 kg of crystalline lactulose and continued concentration by evaporation for a further 40 min at 65-70about2. The method according to p. 1, wherein the seed crystals do not contain water.3. The method according to PP. 1 and 2, characterized in that the evaporation lead to a water content less than 1% by weight of dry matter.
FIELD: biotechnology, biochemistry, enzymes.
SUBSTANCE: invention relates to a method for preparing 4-O-β-D-galactopyranosyl-D-xylose used for in vivo assay of lactase activity in human intestine. Method involves interaction of D-xylose with β-D-galactopyranoside for 2-48 h in a solution at pH 5-9, at temperature that changes from the mixture freezing point to 45°C, and the following addition of 10-1000 U of β-D-galactosidase per 1 g of β-D-galactopyranoside. Reaction is terminated by inactivation of enzyme followed by isolation and crystallization of fraction containing 4-O-β-D-galactopyranosyl-D-xylose in the crystallization mixture chosen from acetone/methanol = (5-1):(20-1) and acetone/water = (5-1):(20-1). Invention provides increasing content of 4-O-β-D-galactopyranosyl-D-xylose in the final mixture.
EFFECT: improved preparing method.
42 cl, 2 tbl, 9 ex
FIELD: cosmetics, pharmacy.
SUBSTANCE: invention relates to associates containing trehalose and calcium chloride as a crystalline monohydrate comprising above said components and water in the molar ratio = 1:1:1, or as anhydrous crystal containing above said components in the molar ratio = 1:2. Indicated crystals of two species of associates of trehalose and calcium chloride can be used in different branches in production of foodstuffs, cosmetic agents and others.
EFFECT: valuable properties of compounds.
3 cl, 20 tbl, 15 dwg, 30 ex
SUBSTANCE: use of two carbohydrate residues in the structure of a divalent neoglycoconjugate enables sixfold increase in binding efficiency compared to the monovalent derivative of monolactosyl-L-glutamate-succinate-dihexadecyl-L-glutamic acid, which can be used to produce high-efficiency directed systems for delivering medicinal agents. EFFECT: high affinity of carbohydrate residues to proteins by forming new divalent ligands based on L-glutamic acid diether, which are specific to corresponding receptors which contain residues of different carbohydrates, via a 1,3-dipolar cycloaddition reaction.
2 cl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as heparan sulphate-binding protein inhibitors. The invention also refers to a pharmaceutical or veterinary composition having heparan sulphate-binding protein inhibitory activity for preventing or treating a disorder in a mammal, and to the use of these compounds and compositions for antiangiogenic, antimetastatic, anti-inflammatory, antimicrobial, anticoagulant and/or antithrombotic therapy in a mammal.
EFFECT: preparing the new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as the heparan sulphate binding protein inhibitors.
10 cl, 31 ex, 11 tbl, 40 dwg
SUBSTANCE: invention relates to an oligosaccharide ingredient for adding to nutritional compositions or food products, which contains glycosylated amino acids and peptides of general formula RnSacm, where R is an amino acid residue, Sac is a monosaccharide selected from a group comprising N-acetyl-neuraminic acid, N-acetyl-galactosamine and galactose, n has a value between 1 and 10 with the proviso that if n equals 1, R is a threonine residue or a serine residue and if n has a value between 2 and 10, the peptide contains at least one threonine or serine residue, m has a value between 2 and 4 and at least 20 mol % of the ingredient is N-acetyl-neuraminic acid, where the oligosaccharide ingredient contains 15-20 mol % N-acetyl-galactosamine, 15-25 mol % galactose, 20-50 mol % N-acetyl-neuraminic acid and 15-25 mol % threonine or serine or mixture thereof.
EFFECT: obtaining an oligosaccharide ingredient.
7 cl, 1 tbl, 2 ex
SUBSTANCE: oligosaccharide ingredient, which is intended to increase content of sialic acid in baby formula, contains glycosylated amino acids and peptides of general formula RnSacm, where R is an amino acid residue, Sac is a monosaccharide selected from a group comprising N-acetyl-neuraminic acid, N-acetyl galactosamine and galactose n has a value from 1 to 10, under the condition that if n=1, then R is a threonine residue or a serine residue and if n has a value between (2) and (10) the peptide contains at least one threonine or serine residue, m has a value from 2 to 4 and at least 20 mol % of the ingredient is N-acetyl-neuraminic acid, where the oligosaccharide component contains 10-25 mol % N-acetyl galactosamine, 10-25 mol % galactose, 20-50 mol % N-acetyl-neuraminic acid and 15-50 mol % threonine or serine or mixture thereof.
EFFECT: obtaining an oligosaccharide ingredient intended for increasing content of sialic acid in baby formula.
6 cl, 2 ex
FIELD: preparative chemistry and technology.
SUBSTANCE: invention relates to a method for separating in fractionating a solution containing betaine and sucrose. Method involves chromatographic fractionation of this solution, nanofiltration and regeneration of fraction enriched with betaine and, possibly, fraction enriched with sucrose. Chromatographic separation is carried out in columns filled with material chosen from cation-exchange and anion-exchange resins, and nanofiltration is carried out with a membrane for nanofiltration chosen from polymeric and inorganic membranes having the limit value of passing through a column from 100 to 2500 g/mole. Solution for fractionation represents a solution prepared from sugar beet, for example, the black syrup solution.
EFFECT: improved method of betaine regeneration.
40 cl, 12 tbl, 3 dwg, 7 ex
FIELD: food industry.
SUBSTANCE: invention relates to food and medicine industry, namely to manufacturing of sugar substitutes for dietary and general health meals. The sugar substitute contains inulin and oligofructose in the proportions of 95:5 - 5:95, mainly 75:25 - 25:75, high intensive sweetener and additionally and/or polyol and/or fructose. Relation of total quantity of inulin and oligofructose in grams and of a product of sugar substitute weight in grams by its sweetness coefficient accounts for 1:2 - 1:7, mainly 1:4 - 1:6. The high intensive sweetener is steviaside, sucralose, aspartame, acesulfame K or their mixtures in the amount sufficient to ensure sugar substitute sweetness coefficient equal to 0.5 - 5.0, mainly from 1.0 to 3.0. Erythrite, sorbite, xylitol, maltitol are used as polyol in the composition of the proposed sugar substitute.
EFFECT: manufacturing of a sugar substitute ensuring the recommended level of inulin and oligofructose in the sweetened products.
4 cl, 10 ex
FIELD: food industry.
SUBSTANCE: proposed method provides using wheat or rye brans from vegetable materials. They are milled, mixed with water in mass ratio of 1:2. Solution with barium hydroxide concentration of 10.8 % kept during 20 minutes is injected into the solution, potassium hydroxide is added till its concentration in the solution is 10 %, mixed during 20 minutes and filtered for separation of manns sediments which is washed by ethyl alcohol or water. Washed sediment is mixed with sodium hydroxide solution, kept during 20 minutes. The mixture is filtered, acidified by acetic acid solution and galactoglucomannan is settled by ethyl alcohol at the filtrate and alcohol ratio of 1:4. The galactoglucomannan sediment is dried. For separation of the galactoglucomannan into the rest of the filtrate, the NaOH solution is injected till its concentration is 15%, kept during 20 minutes at the room temperature, filtered and added the solution of Ba(OH)2 in mass ratio of 1:2, the acetic acid is added in mass ratio of 1:1-1:2. Then ethyl alcohol is added for glucomannans sedimentation, the sediment is separated, filtered and dried.
EFFECT: invention produces two types of mannans: galactoglucomannan and glucomannan with high output percentage.
SUBSTANCE: present invention relates to a catalyst for hydrolysis of cellulose or hydrolysis of cellulose and reduction of products of hydrolysis and a method of producing sugar alcohols from cellulose. Described is a catalyst for hydrolysis of cellulose and hydrolysis of cellulose and reduction of hydrolysis products in which a group 8-11 transition metal is deposited on a solid substrate. Described also is a method of producing sugar alcohols involving: hydrolysis of cellulose in the presence of the catalyst in a hydrogen-containing atmosphere at high pressure and reduction of the product of hydrolysis of cellulose.
EFFECT: easy separation of the catalyst from the product, avoiding the need to regulate pH, neutralise acid or alkali when producing alcohols, possibility of recycling a catalyst without activation thereof, obtaining sugar alcohols directly from cellulose using said catalyst.
19 cl, 2 tbl, 8 dwg, 6 ex
SUBSTANCE: invention relates to method of obtaining xylose product and cellulose for chemical recycling from xylane-containing biomass, which has xylane content 10-35%, calculated per dry biomass substance. In accordance with claimed method prehydrolysis of xylane-containing biomass is carried out with obtaining dissolved hemicelluloses hydrolysate and non-dissolved prehydrolysis biomass, and separation of dissolved hemicellulose hydrolysate from non-dissolved prehydrolysis biomass. After that, carried out are: chromatographic fractioning of dissolved hemicellulose hydrolysate, nanofiltration or crystallisation of sediment for obtaining xylose product, which has xylose content, at least, 55% calculated per content of dry substance of xylose product. After that, boiling of non-dissolved prehydrolysis biomass is carried out by sulphate method to obtain cellulose for chemical recycling with permanganate number lower than 14 and viscosity higher than 600 ml/g.
EFFECT: method ensures obtaining high quality cellulose and xylane.
47 cl, 21 tbl, 12 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry. Disclosed is a method of reducing amount of acrylamide formed during production of molasses, according to which asparaginase is added to clarified sugar solution and water is evaporated. Further, prepared sugar syrup is separated into a crude sugar fraction and a molasses fraction.
EFFECT: method provides considerable reduction of acrylamide content in product without negatively affecting quality and characteristics.
9 cl, 2 dwg, 2 tbl
FIELD: food industry.
SUBSTANCE: invention relates to food industry. Method of producing fructose-glucose syrup from girasol tubers involves washing and grinding girasol, extraction for 15-20 minutes, separation of extract from solid phase, concentration and packaging. After grinding juice is squeezed from washed fresh girasol. After separation of pomace from solid phase, hot water is gradually added at temperature 80-85 °C until obtaining water duty juice/water (1:1)-(1:2), pomace is placed in an ultrasonic bath with heating, sugar is poured with water and extraction is carried out at temperature 70-80 °C. Extract is separated from solid phase by filtration, squeezed in hot form, then filtrate is mixed with water-diluted juice. Method then includes hydrolysis thereof to obtain fructose-glucose syrup by adding a solution of edible citric acid to pH 3.0-4.0 at temperature 80-85 °C for 4-4.5 hours with constant stirring. Hydrolysate is concentrated in vacuum-evaporator at temperature 80-85 °C to content in fructose-glucose syrup not less than 85-90 % of dry substances, then ready syrup is packed into containers.
EFFECT: invention increases dry substances content in fructose-glucose syrup to 85-90% compared to prototype.
1 cl, 1 tbl, 3 ex
SUBSTANCE: provides a method for increasing the availability of carbohydrates in the biomass feedstock. By cutting, grinding, crushing, grinding or chopping reduce the size of the original biomass material containing polysaccharides selected from cellulose, pectin, hemicellulose, and mixtures thereof. Initial biomass, containing less than 5% of water, is obtained. Initial biomass is irradiated by electron beam at a dose level of 1 to 10 Mrad/s and a power of 1 kW to 500 to produce a first processed biomass material. And then it is cooled and again, the treated first biomass material is irradited with an electron beam at a dose level of 1 to 10 Mrad/s and a power of 1 kW to 500 kW. The second processed biomass material is obtained having a number of average molecular weight from 3000 to 50,000 daltons of carbohydrates and increased accessibility.
EFFECT: invention allows to obtain a material having a nutrient availability greater than the nutrient availability of the biomass of the original.
17 cl, 46 dwg, 20 tbl, 45 ex
FIELD: food industry.
SUBSTANCE: method for producing maltitol-containing syrup and applying the above syrup to reduce the catalyst amount at the hydrogenation stage of maltose-containing syrup. The method includes liquefying starch milk, saccharifying the liquefied starch milk, adding maltogenic alpha-amylase or isoamylase to the liquefied starch milk during saccharification, molecular sifting of the maltose-containing syrup to obtain a fraction containing at least 95% of maltose, and the catalytic hydrogenation of said fraction.
EFFECT: obtaining a syrup with the high content of maltitol and the low content of sorbitol and maltotriitol.
11 cl, 2 tbl, 5 ex