The method of obtaining products pornstarsexy

 

(57) Abstract:

The use of biotechnology. The inventive method of obtaining pornstarsexy products, which consists in the fact that the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2 to 10 cSt with further crystallization at a temperature of from minus 20°C to + 20°C, pH of 4.5 - 6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor by dehydration of the mother liquor at 60 - 180°C to obtain a second purenaturaldiva product. table 4.

The invention relates to the production of biologically active substances obtained by microbiological synthesis, and can be used for the production of pornstarsexy products (inosine, guanosine, gipoksantina). These products can be used to obtain Riboxin, adenosine and derivatives based on them, as well as growth-stimulating feed supplements for agricultural animals and poultry.

Known methods for producing pornstarsexy products and nucleosides methods of microbiological synthesis, followed by separation and chemical cleaning.

In the method of obtaining ulturally liquid aluminium sulphate with bringing the pH to 5.2 to 5.5, separation of the supernatant liquid (native solution), removing purines using carbon-based sorbents, concentration by evaporation spirit and the ammonia eluate and crystallization during cooling from the receipt of raw sugar, which is then dissolved and purified getting Riboxin using sulfocationites.

The disadvantages of this and other known methods are:

the low yield of the target products because of the multi-stage purification;

the presence of unusable waste in the form of depleted pornstarsexy solutions and waste in the form of acid-alkaline waste water formed by the solutions used for regeneration of ion exchange resins.

The purpose of the invention is to increase the yield of target products.

For this purpose, the method of obtaining pornstarsexy products by fermentation of strains - producers, branch biomass and concentration, the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2-10 cst with further crystallization at a temperature of from minus 20aboutTo + 20aboutWith a pH of 4.5-6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor with ptx2">

Managed to establish that the concentration of native solution and subsequent crystallization in the selected conditions enable you to get the first pornstarsexy product containing purines 60-90%. Such a simple and effective process due to the fact that crystallization of purines and related impurities essentially depends, on the one hand, the viscosity of the obtained concentrate, on the other hand, temperature and pH during crystallization. Depending on the relative content of purines and impurities in the original native solution selected optimal degree of concentration, which is limited by the limit values of the kinematic viscosity coefficient, and the temperature and pH of crystallization. To study the optimal conditions for the concentration and crystallization are given in table. 1-4. At high content of purines and low impurity concentration degree can be high and crystallization temperature of 0-20aboutWith, otherwise (the inverse ratio of the target product and impurity) concentration degree below the temperature of crystallization - 20-0aboutC.

Thus, by selective crystallization of the target product is holding the product, which can be purified by known methods to obtain inosine (Riboxin), guanosine, gipoksantina or transformed with getting adenosine, ribose and other products based on it.

Remaining from the crystallization of the first mother liquor containing purine compounds, dehydrated at 60-180aboutWith receipt of the second purenaturaldiva of the product in which the content of purines is 5-15% . The specified product can be used as a feed additive. Thus, the yield of the target product rises to 90%, (in the prototype up to 60%), eliminated waste and wastewater salt solutions.

P R I m e R 1. 12 l culture fluid containing at 14.7 g/l Riboxin, 1.5 g/l guanosine and 0.8 g/l gipoksantina was heated to 55aboutWith under stirring and kept at this temperature for 2 hours the Biomass was separated by separation. Biomass volume 1.2 l contained to 15.8 g/l Riboxin, 1.7 g/l guanosine and 1.0 g/l gipoksantina. Got 10,8 l native solution Riboxin content Riboxin 14.5 g/l guanosine 1.5 g/l and gipoksantina 0.9 g/L. Native solution was evaporated on a rotary vacuum evaporator plant at 70aboutC. Received 2,1 l one stripped off native solution Go solution at 20aboutS - 7,5 cSt. The importance of hydrogen ion exponent pH of 5.2. One stripped off native solution slowly (within 6 h) was cooled under stirring to a temperature of minus 4aboutC and kept at this temperature for 8 hours the Precipitated crystals of the first purenaturaldiva product was separated by filtration. First pornstarsexy the product was washed on the filter with 200 ml of demineralized water with a temperature of 2aboutC. Washing was added to the mother liquor after separation of the first purenaturaldiva product. Received 410 g of paste of the first purenaturaldiva product with a humidity of 60% and a content of purines,, Riboxin 121, guanosine 14,8, gipoksantina of 9.3. The paste was dried in a vacuum drying Cabinet at 60aboutC for 3 hours Got 160 g of the first purenaturaldiva product with a moisture content of 1.5%, the total content of purines 142 g (85,5%) including: Riboxin 118 g (71,1%) Guanosin of 14.7 g (8,8%) Gipoksantin 9.3 g (5,6%)

The mother liquor by washing with a volume of 1.9 l containing purines, g/l: Riboxin 16, guanosin 0.8 and gipoksantin 0,6, obezvozhivani on the spray dryer at a temperature of coolant at the entrance to the drying chamber tI= 170aboutWith and at the outlet of the drying chamber to.= 80aboutC. Received 292 g of dry second purenaturaldiva product with Antin 1.1 g (0,40%)

The output of the first purenaturaldiva product by the sum of purine content in culture liquid of 70%. The output of the second purenaturaldiva product by the sum of purine content in the culture fluid of 13.8%.

With the increase of the coefficient of kinematic viscosity one stripped off native solution more than 10 cSt dramatically increases the time of crystallization of the first purenaturaldiva product and reduced output.

With increasing crystallization temperature of more than 20aboutWith dramatically increased loss of product from the mother liquor.

When lowering the crystallization temperature below minus 20aboutWith solidification one stripped off native solution.

With decreasing values of pH the pH is below 4.5 or when increased above 6.0 is a sharp reduction of the yield due to losses from the mother liquor.

The use of the proposed method in comparison with the prototype will allow you to:

partly to solve the environmental problem by eliminating waste production Riboxin and salt water;

get valuable peristeras products on the basis of production wastes;

to increase the yield of target products;

to simplify the technology to reduce the I and concentration of native solutions.

The METHOD of OBTAINING PORNSTARSEXY PRODUCTS by fermentation of the strain-producer, branch biomass, concentration of native solution and crystallization, characterized in that, to increase the yield of the target product, the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2 to 10 CST with further crystallization at a temperature of from -20 to +20oWith a pH of 4.5 - 6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor with subsequent dehydration of the mother liquor at 60 - 180oTo obtain the second purenaturaldiva product.

 

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