The method of obtaining products pornstarsexy
(57) Abstract:The use of biotechnology. The inventive method of obtaining pornstarsexy products, which consists in the fact that the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2 to 10 cSt with further crystallization at a temperature of from minus 20°C to + 20°C, pH of 4.5 - 6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor by dehydration of the mother liquor at 60 - 180°C to obtain a second purenaturaldiva product. table 4. The invention relates to the production of biologically active substances obtained by microbiological synthesis, and can be used for the production of pornstarsexy products (inosine, guanosine, gipoksantina). These products can be used to obtain Riboxin, adenosine and derivatives based on them, as well as growth-stimulating feed supplements for agricultural animals and poultry.Known methods for producing pornstarsexy products and nucleosides methods of microbiological synthesis, followed by separation and chemical cleaning.In the method of obtaining ulturally liquid aluminium sulphate with bringing the pH to 5.2 to 5.5, separation of the supernatant liquid (native solution), removing purines using carbon-based sorbents, concentration by evaporation spirit and the ammonia eluate and crystallization during cooling from the receipt of raw sugar, which is then dissolved and purified getting Riboxin using sulfocationites.The disadvantages of this and other known methods are:
the low yield of the target products because of the multi-stage purification;
the presence of unusable waste in the form of depleted pornstarsexy solutions and waste in the form of acid-alkaline waste water formed by the solutions used for regeneration of ion exchange resins.The purpose of the invention is to increase the yield of target products.For this purpose, the method of obtaining pornstarsexy products by fermentation of strains - producers, branch biomass and concentration, the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2-10 cst with further crystallization at a temperature of from minus 20aboutTo + 20aboutWith a pH of 4.5-6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor with ptx2">Managed to establish that the concentration of native solution and subsequent crystallization in the selected conditions enable you to get the first pornstarsexy product containing purines 60-90%. Such a simple and effective process due to the fact that crystallization of purines and related impurities essentially depends, on the one hand, the viscosity of the obtained concentrate, on the other hand, temperature and pH during crystallization. Depending on the relative content of purines and impurities in the original native solution selected optimal degree of concentration, which is limited by the limit values of the kinematic viscosity coefficient, and the temperature and pH of crystallization. To study the optimal conditions for the concentration and crystallization are given in table. 1-4. At high content of purines and low impurity concentration degree can be high and crystallization temperature of 0-20aboutWith, otherwise (the inverse ratio of the target product and impurity) concentration degree below the temperature of crystallization - 20-0aboutC.Thus, by selective crystallization of the target product is holding the product, which can be purified by known methods to obtain inosine (Riboxin), guanosine, gipoksantina or transformed with getting adenosine, ribose and other products based on it.Remaining from the crystallization of the first mother liquor containing purine compounds, dehydrated at 60-180aboutWith receipt of the second purenaturaldiva of the product in which the content of purines is 5-15% . The specified product can be used as a feed additive. Thus, the yield of the target product rises to 90%, (in the prototype up to 60%), eliminated waste and wastewater salt solutions.P R I m e R 1. 12 l culture fluid containing at 14.7 g/l Riboxin, 1.5 g/l guanosine and 0.8 g/l gipoksantina was heated to 55aboutWith under stirring and kept at this temperature for 2 hours the Biomass was separated by separation. Biomass volume 1.2 l contained to 15.8 g/l Riboxin, 1.7 g/l guanosine and 1.0 g/l gipoksantina. Got 10,8 l native solution Riboxin content Riboxin 14.5 g/l guanosine 1.5 g/l and gipoksantina 0.9 g/L. Native solution was evaporated on a rotary vacuum evaporator plant at 70aboutC. Received 2,1 l one stripped off native solution Go solution at 20aboutS - 7,5 cSt. The importance of hydrogen ion exponent pH of 5.2. One stripped off native solution slowly (within 6 h) was cooled under stirring to a temperature of minus 4aboutC and kept at this temperature for 8 hours the Precipitated crystals of the first purenaturaldiva product was separated by filtration. First pornstarsexy the product was washed on the filter with 200 ml of demineralized water with a temperature of 2aboutC. Washing was added to the mother liquor after separation of the first purenaturaldiva product. Received 410 g of paste of the first purenaturaldiva product with a humidity of 60% and a content of purines,, Riboxin 121, guanosine 14,8, gipoksantina of 9.3. The paste was dried in a vacuum drying Cabinet at 60aboutC for 3 hours Got 160 g of the first purenaturaldiva product with a moisture content of 1.5%, the total content of purines 142 g (85,5%) including: Riboxin 118 g (71,1%) Guanosin of 14.7 g (8,8%) Gipoksantin 9.3 g (5,6%)
The mother liquor by washing with a volume of 1.9 l containing purines, g/l: Riboxin 16, guanosin 0.8 and gipoksantin 0,6, obezvozhivani on the spray dryer at a temperature of coolant at the entrance to the drying chamber tI= 170aboutWith and at the outlet of the drying chamber to.= 80aboutC. Received 292 g of dry second purenaturaldiva product with Antin 1.1 g (0,40%)
The output of the first purenaturaldiva product by the sum of purine content in culture liquid of 70%. The output of the second purenaturaldiva product by the sum of purine content in the culture fluid of 13.8%.With the increase of the coefficient of kinematic viscosity one stripped off native solution more than 10 cSt dramatically increases the time of crystallization of the first purenaturaldiva product and reduced output.With increasing crystallization temperature of more than 20aboutWith dramatically increased loss of product from the mother liquor.When lowering the crystallization temperature below minus 20aboutWith solidification one stripped off native solution.With decreasing values of pH the pH is below 4.5 or when increased above 6.0 is a sharp reduction of the yield due to losses from the mother liquor.The use of the proposed method in comparison with the prototype will allow you to:
partly to solve the environmental problem by eliminating waste production Riboxin and salt water;
get valuable peristeras products on the basis of production wastes;
to increase the yield of target products;
to simplify the technology to reduce the I and concentration of native solutions. The METHOD of OBTAINING PORNSTARSEXY PRODUCTS by fermentation of the strain-producer, branch biomass, concentration of native solution and crystallization, characterized in that, to increase the yield of the target product, the concentration of the native solution is carried out to a coefficient of kinematic viscosity of the concentrate 2 to 10 CST with further crystallization at a temperature of from -20 to +20oWith a pH of 4.5 - 6.0 and separation of crystals of the first purenaturaldiva product from the mother liquor with subsequent dehydration of the mother liquor at 60 - 180oTo obtain the second purenaturaldiva product.
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: the strain-producer is isolated from soft coral Sinularia sp. relating to species Aspergillus fumigatus and deposited in Collection of Marine Microorganisms of Pacific institute of bioorganic chemistry DVO RAN (KMM TIBOKH) at № 4631. Method for preparing indole alkaloids involves surface solid-phase culturing the indicated strain on nutrient medium, milling mycelium and medium, two-fold extraction of prepared mixture with system chloroform - ethanol (2:1), concentrating extract, its chromatography on column with silica gel and the following separation of indole alkaloids by HPLC method. Method based on applying this strain provides enhancing yield of indole alkaloids. Invention can be used in preparing indole alkaloids eliciting an anti-tumor activity.
EFFECT: improved method for preparing, valuable medicinal properties of indole alkaloids.
4 cl, 1 tbl, 2 ex
FIELD: biotechnology, medicine, antibiotics.
SUBSTANCE: invention proposes to the new compound amycomycin of the molecular formula C65H115NO18 (structural formula is given on the invention claim) that shows an antibacterial activity. Amycomycin, its pharmaceutically acceptable salts and derivatives in all their stereoisomeris and tautomeric forms can be obtained by culturing microorganism Amycolatopsis sp. ST 101170 (DSM 12216) under aerobic conditions on the nutrient medium containing the necessary nutrient components. The end product is isolated and purified and converted if necessary to its pharmacologically acceptable salt, ester, ether and other chemical derivatives and eliciting the same spectrum of antibacterial activity. Amycomycin is a component of the pharmaceutical composition eliciting an antibacterial activity. Amycomycin acts as an antibiotic. Invention provides inhibition of microorganisms with resistance to vancomycin and teicoplanin used in treatment of infections caused by Staphylococcus aureus.
EFFECT: improved preparing method, valuable medicinal properties of amycomycin.
7 cl, 2 tbl, 4 ex
FIELD: biotechnology, in particular production of melanin which represents pigment of high antioxidant and anti-tumor activity.
SUBSTANCE: claimed method includes providing by selection of yeast strain Nadsoniella nigra which under deep cultivation accumulates up to 25 g/l of yeast biomass after 96 hours from cultivation starting. Melanin is isolated both from yeast biomass (intracellular melanin) and from cultural liquid (extracellular melanin). Intracellular melanin is isolated by extraction with alkali solution followed by melanin deposition with hydrochloric acid. To isolate extracellular melanin cultural liquid is treated with ethanol followed by melanin deposition with hydrochloric acid.
EFFECT: method if increased effectiveness.
FIELD: biotechnology, biochemistry, enzymes, microbiology.
SUBSTANCE: invention proposes a method for microbiological oxidation of N-, O- or S-heterocyclic mono- or multinuclear aromatic compounds. The method involves culturing the recombinant microorganism that expresses cytochrome P-450-dependent monooxygenase BM-3 from Bacillus megaterium with amino acid sequence represented in SEQ ID NO:2 that comprises at least one functional mutation in region 86-88 and, if necessary, at least one functional mutation in one of regions 73-82, 172-224, 39-43, 48-52, 67-170, 300-335 and 352-356. The prepared oxidation product is isolated from medium, Invention provides carrying out oxidation of organic compounds with enhanced degree of effectiveness.
EFFECT: enhanced effectiveness of enzymes.
17 cl, 1 tbl, 7 ex
FIELD: organic chemistry, biotechnology.
SUBSTANCE: invention refers to biotechnology and can be applied in food industry for sweeteners production. Method of indole-3-pyruvic acid production includes indole-3-pyruvic acid production with enzyme catalyzing transformation of tryptophane to indole-3-pyruvic acid and deaerating treatment or oxygen-removing treatment in acid conditions. And enzyme made of microorganism characterized with amino-acid oxidase activity and catalase activity is used. 4-(indole-3-ilmethyl)-4-hydroxy-2-oxoglutaric acid is made of indole-3-pyruvic acid and oxaloacetic acid and pyruvic acid. Methods are easy realized and enable to produce high yield products.
EFFECT: production of indole-3-pyruvic acid.
15 cl, 17 tbl, 4 ex
SUBSTANCE: invention relates to a method of producing (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid, which includes enantioselective enzymatic reduction of an aryl ketone in a reaction mixture containing oxidoreductase and carbamination of the obtained alcohol.
EFFECT: obtaining (R)-1-aryl-2-tetrazolylethyl ether of carbamic acid.
19 cl, 1 tbl, 5 ex
FIELD: organic chemistry, microbiology, medicine, oncology.
SUBSTANCE: invention relates to novel class of antitumor compounds found in isolation from new marine microorganisms of genus Actinomadura sp., strain PO13-046, namely, to compound denoted as IB-00208: and relates to class of compounds of the formula (I) or (II) , wherein each substitute R1 is similar or different and can represent hydrogen atom, acyl, alkyl, alkenyl, aryl, benzyl, alkaline metal and/or monosaccharide, disaccharide, trisaccharide or their derivative; R2 and R3 can represent hydrogen atom or alkyl. These compounds show antitumor activity.
EFFECT: valuable medicinal properties of compounds.
8 cl, 3 tbl, 9 dwg, 11 ex
FIELD: chemistry; biochemistry.
SUBSTANCE: invention refers to genetic engineering and biotechnology and can be used in food industry. Enzyme chosen from gamma glutamyl hydrolase, GTP cyclohydrolase, dihydroneopterin aldolase and 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase is superexpressed in lactobacillus used in enzyme method for production of monoglutamylpholate and in composition of food, including dairy product.
EFFECT: higher pholate content in bioavailable form.
9 cl, 5 dwg, 2 ex
FIELD: chemistry, biochemistry.
SUBSTANCE: invention relates to field of biotechnology, namely to bacteriochlorophyll derivatives and can be used for medical and diagnostic purposes. Anion-containing water-soluble tetracyclic and pentacyclic bacteriochlorophyll (Bchl) derivatives contain one, two or three negatively charged groups and/or acid groups, which transform into negatively charged groups at physiological pH. Obtained derivatives are used for photodynamic therapy and for tumor diagnostics, as well as for killing cells or infectious agents.
EFFECT: increasing selectivity of binding with target in photodynamic therapy and diagnostics.
25 cl, 34 dwg, 5 tbl, 57 ex
SUBSTANCE: there is recovered a fragment of genomic DNA Pseudomonas fluorescent A2-2, including full-size gene cluster of biosynthesis of safracin (A and B) analysis of which showed the presence of several "OPC" arranged in two operons: sacABCDEFGH and sacIJ. Expression of nucleic acid containing full gene cluster of safracin in a heterosystem enabled producing recombinant forms of natural safracins A and B.
EFFECT: removal or malfunction of separate genes found in operons, and applications of the produced modified forms of nucleic acid in the recombinant DNA technology have resulted in synthesised analogues of safracin to be used as an antimicrobial or antitumour agent, and also in synthesis of ecteinascedine compounds.
20 cl, 9 dwg, 7 ex
SUBSTANCE: invention relates to a method for regiospecific synthesis of rapamycin 42-ester derivatives of formula , where R is ketal isopropylidene substituted with 3-hydroxy-2-(hydroxymethyl)-2-methylpropionic acid, linear or branched C1-C10 alkyl, which possibly contains a halogen, C2-C8 alkenyl, or phenyl, where the said method involves acylation of 42-hydroxyrapamycin with an acyl donor in the presence of lipase. The invention also relates to regiospecific preparation of rapamycin 42-ester from ketal isopropylidene substituted with 3-hydroxy-2-(hydroxymethyl)-2-methylpropionic acid in the presence of lipase.
EFFECT: increased output of the product under mild conditions.
13 cl, 12 ex
SUBSTANCE: method includes preparation of the inoculum of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D). Preparation of the nutrient medium containing one or more sources of carbon, nitrogen, metal ions in the form of soluble salts with fractional feeding of the carbon source during the productive stage. At that, as an additional carbon source the nutrient medium contains rhamnose, and a necessary component of the nutrient medium - MnS04 and sorbent from the group of amberlites XAD. Adding of the inoculate of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D) to the nutrient medium together with rhamnose and MnSO4 in a predetermined ratio and incubating of the medium at pH 7.0-7.5, temperature 25-35°C for 7-10 days. Separation of the residue is carried by centrifugation. The resulting residue is added to ethanol at a predetermined ratio, followed by extraction of tacrolimus at 30 for 2 hours and centrifuging to obtain the extract comprising tacrolimus.
EFFECT: invention enables to increase the yield of tacrolimus.
9 cl, 6 ex
SUBSTANCE: method of fugu toxin production provides for extraction of fugu toxin containing bacteria from the fugu toxin containing carriers, and their location on substratum for cultivation and fugu toxin extraction from the understratum. The bacteria of genus Bacillus are used as containing bacteria, their cultivation is performed to the stage of spore formation under conditions stimulating spore formation. Stimulation of the spore formation to increase fugu toxin yield value can be made, in particular, by use of medium of spore formation adjuvant, by thermal shock, long-term cultivation, by addition of the sporulating salt, nucleosides, bacterial lysates, by bacteria transfer to the distilled water. Wherein prior to fugu toxin extraction from the substratum it is necessary to extract the sporiferous clean strains of bacteria of genus Bacillus, each of them for the fugu toxin cultivation is located on the separate substratum.
EFFECT: invention increases yield value of fugu toxin, and simplifies the method of its production.
6 cl, 3 dwg, 8 ex
SUBSTANCE: structure of nucleic acid is suggested, it contains the cluster of genes of pyripyropene biosynthesis, and marker. The transformant is suggested, it is produced by injection of the above said structure of the nucleic acid to host cell of the filamentous fungus producing pyripyropenes. Also, the transformant is suggested, it is produced by simultaneous or separate introduction of the structure of the nucleic acid containing the cluster of genes of the pyripyropene biosynthesis, and structures of nucleic acid containing the marker, to the host cell of the filamentous fungus producing pyripyropenes. The method of pyripyropenes A, E or O production using the above said transformants.
EFFECT: group of inventions ensures increased production of the said pyripyropenes by transformant in comparison with the parent-cell.
10 cl, 7 dwg, 5 tbl, 20 ex
SUBSTANCE: group of inventions relates to biochemistry. Disclosed are methods of producing pyripyropene A, characterised by cultivation of a transformed microorganism, in which there is at least one specific polynucleotide or a recombinant vector comprising it/them introduced with pyripyropene E and isolating pyripyropene A through pyripyropene O or with deacetyl pyripyropene E and isolating pyripyropene A via pyripyropene E and pyripyropene O.
EFFECT: group of inventions enables to obtain said pyripyropenes by genetic recombination, making a significant contribution to technology of large-scale production of pyripyropenes A, E, O.
9 cl, 12 dwg, 7 tbl, 19 ex
SUBSTANCE: presented variants of tetrapyrrol compound production and bacterial cell for its production. Method envisages cultivation of Escherichia coli bacterial cells in culture medium to produce target compound from culture medium. At that, said compound the structure of phthalocyanine ring carrying 4 methyl group and ethyl ester group 4 or propionate groups on porphyrin ring. E. coli is selected from group consisting of e.coli K12, e.coli BL21, e.coli JD23504, which are able to express the gene ypjD (b2611) as result of transposon ypjD gene (b2611) insert into gene of e.coli cell. When implementing versions of method culture medium is used containing elementary Mn, Au, Cu, Zn or Ru able to be transformed into appropriate ions in culture medium, or Mn-containing, Au-containing Cu-containing Zn-containing Ru-containing compound capable of dissociating on corresponding ions in culture medium. Formed as a result of collecting or release from culture medium tetrapyrrol compound has structure of phthalocyanine ring in centre of which coordinated Mn, Au, Cu, Zn or Ru.
EFFECT: invention enables to obtain tetrapyrrol connection without using its precursor.
4 cl, 22 dwg, 6 ex