A method of reducing nitrogen excretion in humans and animals when feeding protein-containing product, a food and feed product

 

(57) Abstract:

The invention relates to the satisfaction of human needs. Summary of the invention in that to reduce nitrogen excretion in humans and animals when feeding protein-containing product of the incoming protein replaced by alphaketoglutarate acid; a combination of alphaketoglutarate acid and pyruvic acid, oxaloacetate or oxaloacetate in combination with alphaketoglutarate acid or pyruvate, or other ketokislot with the formula R CO-COOX, where R is the share of non-essential amino acids; X is hydrogen, alkali metal, alkaline earth metal or organic base, or a non-toxic intermediate or preceding their products, and the ketoacid (keto acid) present (present) in an amount of 1-10 wt. % and preferably 1-10% by weight of the dry protein. 2 C. and 10 C. p. F.-ly, 6 ill. , 6 PL.

The invention relates to a food and feed product with a content of ketoacids for people and animals.

The closest technical solution is a method of treatment of liver diseases using therapeutic preparations containing ketoanalogue amino acids.

This method involves partial replacement of the material or parenteral administration.

The purpose of this invention to provide food and feed products with a reduced content of protein, satisfying the need of animals in receipt belokoraya and energy-supplying substances. In addition, the food product should satisfy the need in receipt of biomolecules obtained from the nonessential amino acids.

Another aim of the invention is to reduce nitrogen excretion from the body.

The method is illustrated in Fig. 1 - 6.

The background to the invention.

In their natural habitats, many carnivores consume food that contain 30 - 55% protein (degree of substitution 90% ), as an example, salmon (aquatic habitat) and mink (terrestrial environment). The choice of feed is determined by the type of animal and its life phase. A significant proportion of the incoming protein can be oxidized with the formation of the energy-supplying compounds, for example, the adenosine triphosphatase (ATP), resulting in the release of nitrogen into the environment. In the case of intensive cultivation of fish and mink may have problems with pollution.

In natural conditions omnivores consume food that contains 15 to 30% WHI/P> Among the body tissues with the highest energy demand, should be called muscle energy intake from carbohydrate food comes directly from blood or from the liver through the blood as glucose. Glucose is formed in the liver from protein components. In this case the formation of glucose is derived primarily from alanine and its keto acid. Studies have shown that the corresponding keto-acid and some amino acids, for example, alphaketoglutarate (CS), can be precursors of amino acids, such as leucine KIC.

(I) NH4+connected with amino acids during the reaction

NH4++ KG + NADPH = Glutamate +

+NADP++ H2O

In the synthesis of many essential amino acids glutamate gives the alpha amino group:

(II) carbon skeletons that exist at the time of synthesis of essential amino acids are the intermediate products of glycolide, pentose-phosphate shunt or zinc, citric acid and therefore can be produced from pyruvate or KG (see Fig. 5).

(III) at physiological stress, excessive intake of energy, reproduction and other low-energy situations, the oxidation of amino acids increases, which is diaminononane with KG or the AET important role in the hydroxylation of Proline.

The Central metabolism is pyruvate (see Fig. 4), where we can distinguish the following stages:

(I) the formation of lactate to conduct glycolide with NAD+under aerobic conditions;

(II) formation of acetyl-CoA for fatty acid synthesis or absorption in the citric acid cycle.

(III) a high concentration of acetyl-CoA increases the formation of oxaloacetate, which either causes a citric acid cycle intermediate products (at low concentrations adenosinetriphosphatase), or becomes a breeding ground for gluconeogenesis (at large concentrations adenosinetriphosphatase),

(IV) during gluconeogenesis pyruvate may contribute to the pentose-phosphate shunt with the formation of NADPH (is of great importance in some anabolic processes such as the synthesis of the substituted amino acids), and ribose-5-phosphate, which serves as a nutrient medium for the synthesis of nucleotides;

(V) the formation of alanine and other nonessential amino acids, where pyruvate promotes the formation of carbon cores.

Studies have shown the ability to reduce nitrogen excretion by humans and animals, while maintaining the production of proteins by replacing part of the protein in food and feed product through D. the global acid and pyruvic acid, oxaloacetate or oxaloacetate in combination with alphaketoglutarate acid or pyruvate, or other ketokislot with the formula R-CO-COOX, where R is the share of non-essential amino acids, and X is hydrogen, alkali metal, alkaline earth metal or organic base or a non-toxic prior or intermediate their product.

The keto acid can in General be described by the formula R-OO-COOX, where R is the share of non-essential amino acids, and X is hydrogen, alkali metal, alkaline earth metal or organic base or a non-toxic prior or intermediate their product. For simplicity, all these compounds will hereinafter be called keto acid. The number of these ketoacids is 1 to 20 wt. % and preferably from 1 to 10% by weight of dry matter of incoming proteins.

The invention can be applied when power of carnivores, omnivores and herbivores to reduce nitrogen excretion, as well as to conserve protein.

In accordance with this invention, the substrate is in the form of ketoacids nonessential amino acids supplied for the production of proteins or other biomolecules produced from these amino acids. The presence of ketoacids, which during the exchange of substances under aerobic conditions may enter mitochon the La active life processes. During ketoacid metabolism in mitochondria intermediate products are formed, for example, succinyl-COA. Succinyl-COA is present during the synthesis of molecules of porphyrin in hemoglobin and myoglobin. When the organism is added to a keto acid, there is an effect savings protein, because the body can use keto acid for nonessential amino acids instead of using amino acids for purposes other than the production of proteins. This increases the efficiency of protein supplements. The Central position when linking NH4+and subsequent synthesis of essential amino acids is KG, one of the above ketoacids. A more effective substrate in comparison with the amino acids may be different keto-acid non-essential amino acids because there is no need to diaminononane, and reduced volatile nitrogen from the body. The following example 1 shows that when feeding young fish product with the addition of 10% ketoacids decreases nitrogen excretion by more than 20% .

The invention may be useful in parenteral nutrition in the case of catabolic situations, e.g. for larger injuries, severe burns, and so on , and which you can add a keto acid, for example, pyruvate, optionally combining it with KG. In the result, it is possible to avoid a large loss of muscle protein. In addition, decreases the excretion of nitrogen.

P R I m e R 1. Test for growth. For 5 weeks annual salmon fry were fed partially control product designed for very young salmon, partially feedstuff according to this invention, in which lay the same materials as that of the control product, but containing 5% pyruvate sodium salt and 5% of alphaketoglutarate acid (KG), resulting in 10% protein in the diet of fish have been replaced by a keto acid. Under test 500 fingerlings in one pool received control feed, and 500 fingerlings in another pool were fed according to this invention. In the beginning of the test the average weight of fry was approximately 4, during the test, controlled growth, the nitrogen, the General analysis and the analysis of proteins. Results macroscopic studies of fry are given in table. 1 - 3.

Thus, the content of ketoacids in the test food product was 10% , they were replaced 20% of the amino acids and reduced the nitrogen content of approximately 16% . Amino acid analysis of the feed product and fish is the t of the three analyses, based on each 50 fry. Differences between groups not significant.

Tests on the excretion of nitrogen.

Test the release of nitrogen from each group was selected 10 of the fry and put them in an indoor pool filled with oxygenated water. In this environment fry lived 90 minutes, then they were cut, weighed and analyzed. The stomachs and intestinal tracts of all fry were filled with food. Analysis on the allocation of NH3and NH4+was conducted on water samples from both basins, the dierence in time between feeding, killing, and the final sample was the same. The analysis of ammonia was carried out in accordance with guidance from the book of Parsons and other "Manual of chemical and biological methods of analysis of sea water", ed. Pergamon Press, pages 15-16 (ISBN-0-08-030 288-2). The final test was conducted after the fish have reached the age of 20 weeks. Differences in mortality between the control and the test group for additional study periods were observed. The results are presented in table. 4.

The difference in n excretion between groups is 28% , the statistical significance according to t-test Student ravni between both groups regarding the state of biscuits, gastrointestinal filling and accumulation of fat.

The weight of fry in the control group at the beginning of the test was equal to the average 3,40 g, and at the end of 3.95 g, whereas the weight of the treated group feed according to this invention was on average equal to 3.56 g at the beginning and 4.11 g at the end. Growth was also the same.

P R I m m e R 2. In Haute test on rats according to the method of Eggum (Eggum C. O. , "a Study of some factors affecting the utilization of proteins in rats and pigs", the national Institute of zootechnics, 1973, S. 406) conducted a comparison of the feed, consisting of cereals, other carbohydrates, fish protein, fat, minerals and vitamins, with invented food, where to control the stern instead of 1.4 weight % fish protein and 0.15 wt. % soybean oil added to 0.6 weight % pyruvate and 0.8 wt. % alpha Ketoglutarate in order to compensate for the difference in fat content resulting from the reduction of the content of fish protein, with the following results were obtained.

The test is conducted in such a way that the total amount of feed (g DS) in both groups of animals were equal, showed no significant differences in growth despite the fact that the 1.4 weight % protein control feed were replaced by a keto acid.Amy to build their own protein, varies significantly in both groups mainly because of the great biological value (BV) in the test group. The effect of improving the protein in this case is explained by the addition of alpha Ketoglutarate in combination with pyruvate.

Macroscopic examination of the liver, kidneys and other internal organs have not indicated any differences between the two groups of animals.

In order to avoid electrolytic disorders, you should limit the amount of salt in the feed according to this invention, where a significant proportion of the protein replaced ketoacids. There could be other salts ketoacids instead of the sodium salt, for example, salts of potassium, magnesium and calcium. Can also be used free of acid. (56) U.S. Patent N 4100293, class A 61 K 31/40, 1978.

1. A method of reducing nitrogen excretion in humans and animals when feeding protein-containing product by use of a diet containing as a substitute for the protein ketoanalogue amino acids, characterized in that as its keto-analog of the amino acids used or alphaketoglutarate acid, or a combination of alphaketoglutarate acid with pyruvic acid or oxaloacetate, or oxaloacetate in combination with alphaketoglutarate acid, or Perova>X is hydrogen, alkali metal, alkaline earth metal or organic base,

or non-toxic precursors, or their intermediate products, and ketoanalogue acids are contained in amounts of 1 to 20 wt. % protein, counting on the dry matter.

2. The method according to p. 1, characterized in that ketoanalogue amino acids are contained in amounts of 1 to 10 wt. % protein, counting on the dry matter.

3. Food and feed product for humans and animals containing ketoanalogue amino acids, characterized in that as its keto-analog of the amino acids selected: or alphaketoglutarate acid, or a combination of alphaketoglutarate acid and pyruvic acid, or oxaloacetate, or oxaloacetate in combination with alphaketoglutarate acid or pyruvate or other ketokislot with the General formula

R-CO-COOH,

where R is the share of non-essential amino acids;

X is hydrogen, alkali metal, alkaline earth metal,

or organic base, or a non-toxic precursors, or their intermediate products, and ketoanalogue amino acids are contained in amounts of 1 to 20 wt. % protein, counting on the dry matter.

4. The product under item 3, characterized in that ketoanalogue amino acids stereo its keto-analog of the amino acids used alphaketoglutarate acid.

6. The product under item 3, characterized in that the two keto-acid are mixed in a mass ratio of 25 : 75 to 75 : 25.

7. The product under item 3, characterized in that the two keto-acid are mixed in a mass ratio of 40 : 60 to 60 : 40.

8. The product under item 3, characterized in that as its keto-analog of the amino acids used a combination of pyruvic acid and alphaketoglutarate acid.

9. The product under item 3, characterized in that as its keto-analog of the amino acids used oxaloacetate.

10. The product under item 3, characterized in that as its keto-analog of the amino acids used a combination of oxaloacetate and alphaketoglutarate acid.

11. The product under item 3, characterized in that as its keto-analog of the amino acids used a combination of oxaloacetate and pyruvic acid.

12. Product PP. 3 to 8, characterized in that as its keto-analog of the amino acids used in the acid and/or salts of alkaline metal, alkaline earth metal or organic base.

 

Same patents:

The invention relates to the chemistry of condensed heterocyclic systems and specifically relates to new compounds - 4-acetyl-3-benzyl-2-methylthiopyridine(4,5-b)indole of the formula I

< / BR>
Through the study of its biological activity is established that it has the property to protect the liver from carbon tetrachloride poisoning

The invention relates to new derivatives of 2-(21-cyano-31-dialkylamino-21- enylidene)indolinone-3 of the General formula I

(I) where R= R1= CH3(Ia),

R + R1= (CH2)5(IB) having antihypertensive activity

The invention relates to medicine, namely to obstetrics

FIELD: veterinary medicine.

SUBSTANCE: preparation has riboxin, glycolysis activator, vitamin B12, ascorbic acid, citric acid and glucose. The preparation calcium glycerophosphate as the glycolysis activator and additionally has succinic and folic acid and fructose taken in the following ingredient proportions in % by mass: riboxin -10,0-40, calcium glycerophosphate - 30,0-40,0, vitamin B12 - 0.02-0.04, ascorbic acid - 2-4, citric acid - 2-4, succinic acid - 2-4, folic acid - 1-2, fructose 5-10, glucose to 100.

EFFECT: increased laying ability of hens; reduced fodder consumption.

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with preparations and method for treating parasitosis in animals. The suggested curative-fodder additive contains, weight%: univerm 0.5-1.0; albendasol 0.05-0.1; fodder filler as concentrated feedstuffs and water - the rest. Water should be introduced into the additive to achieve 25-30% moisture of the mixture. While carrying out antiparasitic treatments animals should be fed with an antiparasitic preparation in its granulated preparative form in the dosages of 1.5-7.0 g/kg body weight, once for 3 d. Application of the present innovation enables to increase therapeutic efficiency and widen action range of antiparasitic treatments in animals.

EFFECT: higher efficiency.

3 cl, 6 ex, 5 tbl

FIELD: animal science, swine breeding.

SUBSTANCE: during the period of growing and fattening the youngsters for the purpose of increasing the performance in youngsters in case of simultaneous deficiency of selenium and iodine it is necessary to supplement the diet with microadditives. According the technique mentioned one should apply potassium iodide introduced subcutaneously at the dosage of about 6-9 mg/swine on the 60-90th d of their life, and sodium selenite introduced perorally at the dosage of 0.5 mg/kg feedstuff during the whole period of raising. The innovation enables to increase average daily body weight gains by 20.5%, shorten the age to achieve body weight of 100 kg by 24 d and decrease the expenditure of feedstuff per 1 kg body weight gain by 17.2%.

EFFECT: higher efficiency.

2 tbl

FIELD: agriculture, in particular, preparing of feeds for animals.

SUBSTANCE: method involves steaming-through raw material impregnated with acid solution; grinding raw material and delivering into reactor; sealing reactor; holding raw material under reduced pressure in reactor; feeding hot acid solution into reactor; providing impregnation of raw material with acid solution and hydrolysis under excessive pressure during time period of up to 3 hours; maintaining temperature of 85-100 C in reactor during hydrolysis process; upon termination of hydrolysis process, drawing acid solution from reactor through pipeline into working reservoir, wherein neutralizing acid using reactant having acidity of 6.0-7.0; drawing neutralized liquid from reservoir back into reactor and repeatedly impregnating raw material with liquid; holding raw material within reactor during 5-25 min; drawing liquid from reactor into reservoir and controlling its acidity; in case of occurrence of acidic reaction, providing liquid neutralization until pH value is 6.0-7.0; drawing liquid back into reactor containing raw material and impregnating the latter with liquid; repeating cycle until neutrality is stabilized within the range of pH=6.0-7.0 and drawing working liquid into reservoir; unsealing reactor; withdrawing and using resultant product as animal feed. Apparatus has reactor formed as reservoir with cover to be opened or hermetically closed, and working reservoir with cover to be opened and hermetically closed. Vacuum type delivery pump, hot water and gas generator are connected to reactor and working reservoir through pipelines equipped with locking-and-adjusting valves. Reactor and working reservoir are furnished with controlling and measuring instruments and safety-bypass valve.

EFFECT: improved quality of feeds and reduced power consumption.

6 cl, 1 dwg

FIELD: fodder production.

SUBSTANCE: the suggested method deals with reducing green plants and treating them with a filler and fodder additives with pressure and temperature, moreover, the treatment mentioned should be fulfilled in a press-extruder at pressure ranged 20-30 kg/sq.cm and temperature being about 60-80°C. with zeolite at the quantity of 0.5% against fodder's mass and ammonium carbonate at the quantity of 0.5% against fodder's mass. The innovation enables to increase fodder's nutritive value and prolong its storage period.

EFFECT: higher efficiency.

4 ex, 1 tbl

FIELD: fodder production.

SUBSTANCE: method for preparing fodder for ruminant animals involves milling and extrusion of grains and carbamide. As grains method involves using rye and sorghum that are wetted with carbamide solution before extrusion wherein 10-11 l of water at temperature 70-80°C and 10-11 kg of carbamide are used per 100 kg of grains. Extrusion is carried out at humidity 19-22%, temperature 130-140°C and under pressure 3.5-4.0 MPa. Method provides decreasing toxicity of carbamide, elimination of anti- nutritive substances from rye and sorghum grains, decreasing hydrolysis of proteins in rumen of ruminant animals.

EFFECT: improved preparing method of fodder.

2 tbl

FIELD: agriculture.

SUBSTANCE: invention concerns agriculture, and feed production particularly. The method of complete feed stabilisation involves addition of a stabiliser - IFO-6ET (6-ethoxy-2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline) in the ratio of 50 to 150 grams per ton. Thus, when cattle feeds on such feed it is less affected by negative influence of lipo- and hydroperoxide products.

EFFECT: inhibition of oxidation of nutrient and biologically active substances of the feed.

3 tbl, 1 ex

New application // 2353362

FIELD: medicine.

SUBSTANCE: invention refers to medicine and veterinary science and concerns applications of 4-trifluoromethylbenzyl ester (S)-3-[3-(1-carboxy-1-methylethoxy)phenyl]piperidine -1-carboxylic acid in preparation of a medical product for palliative, preventive or medicinal therapy of negative energy balance in ruminants, where said disease is chosen from fatty liver syndrome, allotopia, dysimmunity, immunosuppression, toxification, primary and secondary ketosis, cow mortality syndrome, digestive disorder, appetite loss, retained placenta, slipped abomasum, mastitis, (endo-)-metritis, barrenesses, low fertility and claudification.

EFFECT: invention ensures high clinical effectiveness.

6 cl, 4 dwg, 8 tbl, 13 ex

FIELD: food industry.

SUBSTANCE: invention concerns feeding of pets. Fluid composition is proposed for oral consumption for assistance of increased water consumption containing fermentatively processed liver in quantity from 1 to 20 % wt mixed with water. Method of pet feeding with mentioned composition.

EFFECT: invention allows to increase speed of urine production as preventive measure of disorder of urinary tract lower department.

12 cl, 5 tbl, 5 ex

FIELD: agriculture.

SUBSTANCE: invention refers to veterinary. Forage composition contains the following components: riboxinum, calcium glycerophosphate, fructose, methyluracul, vitamin B12, ascorbic acid, citric acid, succinic acid, folic acid, glucose, chitosan, olygofructose and inuline at ratio 30/70, sources of protein, fats and poly- non-saturated fat acids at specified ratio of components. As components retarding lipogenesis in liver forage also contains: phospholipids - lecithin, L-carnitine, and β-carotene. As protein sources forage also contains soya bean flour, soya bean ground oil cake, and rape-cake. Also as sources of fats and poly-non-saturated fatty acids forage contains fish oil and linseed oil.

EFFECT: forage provides fortification of immunity and can be used for accelerated growth and for retarding development of liver hepatosis of agricultural and domestic animals and birds and fish.

4 tbl, 3 ex

FIELD: food raw and industry.

SUBSTANCE: invention relates, in particulate, to new nutrient sources of trace elements with using the milk proteins hydrolyzate enriched with trace elements. The nutrient chelate complex comprising the milk proteins hydrolyzate and trace elements bound to chelate compounds comprises trace elements in the range of concentrations 1-30 mg/g being from that at least 90% are in an organic form. Invention provides the preparing the nutrient source of trace elements with high assimilability of its components, improved functional properties and reduced adverse unfavorable effect in overdosing due to the elevated content of trace elements in organic form in its composition.

EFFECT: valuable properties of complex.

3 cl, 6 ex

FIELD: food raw and industry.

SUBSTANCE: invention relates, in particulate, to new nutrient sources of trace elements with using the milk proteins hydrolyzate enriched with trace elements. The nutrient chelate complex comprising the milk proteins hydrolyzate and trace elements bound to chelate compounds comprises trace elements in the range of concentrations 1-30 mg/g being from that at least 90% are in an organic form. Invention provides the preparing the nutrient source of trace elements with high assimilability of its components, improved functional properties and reduced adverse unfavorable effect in overdosing due to the elevated content of trace elements in organic form in its composition.

EFFECT: valuable properties of complex.

3 cl, 6 ex

FIELD: food raw and industry.

SUBSTANCE: invention relates, in particulate, to new nutrient sources of trace elements with using the milk proteins hydrolyzate enriched with trace elements. The nutrient chelate complex comprising the milk proteins hydrolyzate and trace elements bound to chelate compounds comprises trace elements in the range of concentrations 1-30 mg/g being from that at least 90% are in an organic form. Invention provides the preparing the nutrient source of trace elements with high assimilability of its components, improved functional properties and reduced adverse unfavorable effect in overdosing due to the elevated content of trace elements in organic form in its composition.

EFFECT: valuable properties of complex.

3 cl, 6 ex

FIELD: medicine, pediatrics.

SUBSTANCE: invention relates to improved compositions for children nutrition. Composition for children nutrition comprises protein component with the content of phosphorus less 0.75 g per 100 g of protein and lipid component easily digested by baby wherein palmitic acid residues are above 10% of total fatty acid residues presenting in triglycerides, and the content of triglycerides with residue of palmitic acid at position sn2 is at least 30% of indicated palmitic acid residues. Method for preparing protein hydrolyzate involves hydrolysis of the parent protein material with the content of phosphorus less 0.75 g per 100 g of protein with combination of trypsin, at least one endoproteinase and at least one exoproteinase. Invention provides preparing the composition for children nutrition providing reducing constipations, discomfort in stomach region and gastroenteric disorders.

EFFECT: improved preparing method, valuable properties of composition.

23 cl, 5 ex

Up!