|
Method comprises isolation of neutrophilic granulocytes from venous blood of cattle by centrifugation in a density gradient of ficoll-urografin (density 1.077 g/cm3), application of 100 mcl of the cell suspension in two parallel wells of 96-well flat-bottomed plate for immunoassay. In one well of the plate 50 mcl of buffered saline or saline (spontaneous NBT-test) are added, and in another - 50 mcl of stimulator (BCG) at optimal dilution in buffered saline, or saline (induced NBT-test), then in both wells of the plate 50 mcl of 0.15% solution of nitroblue tetrazolium is added, stirred, incubated for 20 min at 37°C, centrifuged at 500 g for 10 minutes. Then the supernatant is separated, 200 mcl of absolute ethanol is added. The solution is centrifuged for 5 minutes at 500 g, the supernatant is separated. The packed cells is added to 200 mcl of saline. Centrifuged for 5 minutes at 500 g. 130 mcl dimexide is added to each well and incubated at 60°C for 20 minutes, 70 mcl of 2M KOH solution is added, mixed thoroughly. The results of the reaction are fixed through immunochemical analyser and are expressed in arbitrary units of optical density. After registering the reaction the stimulation coefficient is determined, and in determining the level of induced activity 50 mcl of levamisole is additionally added into the well at a final concentration of 0.5 mcg/ml. Results of the reaction are determined by the difference of the extinction at a wavelength of 630 and 490 nm. The stimulation coefficient (SC) is determined by the ratio of the induced level of cell activity to the spontaneous level. At SC≤0.95 the animal is considered sensitive to leukaemia infection of cattle. |
|
|
Enzyme immunoassay is conducted to determine a quantitative level of patient's oral fluid biomarkers prior to or at least 30 minutes after meals. The biomarker is matrix metalloproteinase (MMP 8) or matrix metalloproteinase (MMP 9). For a clinical reference group, MMP 8 is taken at 21.65-41.85 ng/ml; if the level is 171.25-524.7 ng/ml, a low adaptability is diagnosed, whereas the value of 58.7-162.7 ng/ml shows a complete functional adaptation of the patient suffering dentofacial new growths to removable palatal dentures. For a clinical reference group, MMP 9 is taken at 134.9-207.7 ng/ml; if the level is 526.5-919.1 ng/ml, a low adaptability is diagnosed, whereas the value of 236.8-492.3 ng/ml shows a complete functional adaptation of the patient suffering dentofacial new growths to removable palatal dentures. |
|
|
Invention represents a method for qualitative determination of an adaptability to prosthetic bridges based on the oral fluid biomarkers content in a patient with dentofacial new growths; if the oral fluid contents of tissue inhibitor of metalloproteinase 1 (TIMP 1) in an amount of 118.9-145.7 ng/ml and tissue inhibitor of metalloproteinase 2 (TIMP 2) in an amount of 42.4-56.9 ng/ml are detected, the low adaptability of the patient with dentofacial new growths to prosthetic bridges is diagnosed, whereas the oral fluid contents of TIMP 1 in an amount of 68.2-77.4 ng/ml and TIMP 2 in an amount of 21.9-37.7 ng/ml show the complete functional adaptation of the patient with dentofacial new growths to prosthetic bridges. |
|
|
Muteins of human lipocalin 2 (lcn2, hngal) with affinity to certain target Invention relates to the field of biochemistry, in particular to a method of obtaining muteins of human lipocalin 2 (Lcn2, hNGAL). The method is based on the mutagenesis of a molecule of a nucleic acid, coding the human Lcn2 protein. At least a nucleotide triplet, coding position 96 in a linear polypeptide sequence of the human Lcn2 is subjected to the mutagenesis. The muteins, obtained by the claimed method, which bind with a preliminarily specified target with identifiable affinity, where the said target does not bind with natural hGAL under physiological conditions are also claimed. |
|
|
Diagnostic technique and kit for diagnosing glomerulonephitis in cat Group of inventions refers to biotechnology. A diagnostic technique for glomerulonephritis in a cat provides measuring an expression level of one or more biomarkers specified in a group consisting of lumican; a collagen alpha 1 chain (III), version 12; decorin; secreted related frizzled protein 2; retinol-binding protein 5; MMP-2; MMP-7 and MMP-19 in a biological sample taken from the cat. A considerable difference in the expression of one or more biomarkers in the sample as compared to the reference expression in the sample taken from a healthy animal testifies to the presence of a renal disorder. The group of inventions also contains a kit for implementing the above technique, also applicable for diagnosing nucleotide sequences complementary to the above genes. |
|
|
According to the invention, the method comprises administering of diet to canines, comprising based on the dry weight: DHA+EPA 0.5-2.5%, vitamin C 75-1000 mg/kg, vitamin E 250-1000 mg/kg, L-carnitine 100-1000 mg/kg. The composition of the invention comprises based on the dry weight: DHA+EPA 0.5-2.5%, vitamin C 75-1000 mg/kg, vitamin E 250-1000 mg/kg, L-carnitine 100-1000 mg/kg. |
|
|
Probability of injury to normal tissues, adapted with account of markers Group of inventions relates to medicine, oncology and deals with the formation of a patient's individual therapy plan. The method includes the formation of an initial plan of therapy by means of a normal tissue complication probability (NTCP) model and a tumour control probability (TCP) model for a target area. NTCP and TCP models are adapted on the basis of initial quantitative values of the patient's set of biomarkers. After the application of the initial therapy a corrected plan of therapy is formed by means of NTCP and TCP models, adapted on the basis of updated after the application of therapy quantitative values of the set of biomarkers. The initial and updated NTCP models are expressed in the form of the function of an equivalent uniform dose (EUD) modified with the application of a scalar value. The values of the set of biomarkers represent determined by an analysis values of parameters from the group: levels of Hb, CRP, PSA, TNF-α, ferritin, transferritin, LDH, IL-6, hepsidin, creatinine, glucose, HbA1c, complexes, binding DNA ends (DNA-EBC), HIF-lα, galectine-1, CAP43 and/or NDRG1; length of a telomere; type of the tumour; tumour degree; tumour stage; location of the initial tumour; degree of malignancy by the Gleason scale; data of the collagen level analysis; previous treatment in the form of abdominal surgery, hormonal drug therapy, anticoagulation drug therapy; presence of diabetes; patient's age. A machine-readable data carrier, which contains a programme, a controlling processor for carrying out the said method and a processor for carrying out method stages, is used. |
|
|
Shear device for shearing test of samples of fine-grained cohesive and non-cohesive soil and snow Shear device is used for shearing test of samples of fine-grained cohesive and non-cohesive soil and snow. The device comprises two vertical holders arranged coaxially. One of the holders is made stationary, and the other - with the possibility of horizontal movement under the action of horizontal shear force loading. In the inner area of the movable holder at least one sectional sample divider of the test body is fixedly located, which is made in the form of a vertical plate located perpendicularly to the direction of action of the horizontal shear force loading. The sample of the test body is placed inside. The presence of sectional soil dividers provides the condition when the shearing force is perceived by the entire the cross-sectional area of the holder, not by its separate part. |
|
|
Method of determination of fire resistance of brick columns with ferroconcrete holder Brick columns with ferroconcrete holder are tested without destruction by a complex of single parameters of quality, estimating the value of the actual degree of fire resistance by the loss of bearing capacity. For this purpose the geometrical sizes of brick columns and ferroconcrete holder, condition of heating of columns, the buckling coefficient, classes of concrete and reinforcing steel, their compression resistance, parameters of thermal diffusion of materials of concrete of the holder and the brickwork; the value of standard load during fire resistance test, degree of tension of hazardous sections of ferroconcrete holder and the brickwork. The degree of fire resistance of brick columns with ferroconcrete holder is determined by polyparametrical relations describing the process of resistance of stone structure to fire effect. |
|
|
Method of determining fire resistance of stone pillars with steel becket Testing of stone pillars with steel becket is conducted without destroying on the complex of individual quality indicators, assessing the value of the actual fire resistance limit on load-bearing capacity loss. To do this, the geometric dimensions of the stone pillars with steel becket is determined, as well as bearing conditions and the structure heating, the value of buckling coefficient, values of density, moisture content, thermal conductivity, heat capacity and thermal diffusion of the material of stone pillars with steel becket, the percentage of reinforcement with connecting plates of the steel becket; the value of standard loads in testing the fire resistance and the degree of tension of dangerous cross-sections of the stone structure. The fire resistance limit of stone pillars with steel becket is determined by the polyparametric mathematical relationship. |
|
|
Method of treating patients with rhinosinusitis Patient's pre-therapeutic blood serum is analysed to measure a substance P level. If the substance P level is 2,000 pg/ml and more, a common therapeutic regimen is added with preparations Prednisolone and Milgamma from the first therapeutic day during five days. Prednisolone in a dose of 30 mg is administered orally once a day. Milgamma is injected intramuscularly in a dose of 2 ml once a day. |
|
|
Indicator element for detecting leakage of liquid hydrocarbon fuel Indicator element comprises a substrate, an indicator and a white absorbent material attached to the substrate, and the indicator is made of finely dispersed dye which is soluble in liquid hydrocarbon fuel but insoluble in water, and is placed between the substrate and the white absorbent material, wherein the substrate is a waterproof opaque film with a sticky layer. |
|
|
Method for prediction of bronchial asthma exacerbation Peripheral blood is taken at the disease-free stage. The taken blood is divided into two samples. One sample is exposed to ultraviolet light for at least 60 seconds. They are used to prepare blood serum samples which are analysed by marginal dehydration in polarised light. If observing aggregations of a spherolith crystal and an integrated ball of small weakly anisotropic granules in the unexposed sample, and spherolith crystals surrounded by loose clusters of weakly anisotropic granules in the exposed sample, the bronchial asthma exacerbation is predicted. |
|
|
Biomarker for alzheimer's disease or mild cognitive impairment Invention relates to the field of medicine, namely to a method of diagnosing Alzheimer's disease or a mild cognitive impairment. The essence of the method consists in the fact than the method includes the measurement of desmosterol, beta-amyloid, and gelsolin in blood. If the desmosterol level in the subject's blood is below the standard value, or if the desmosterol level and Aβx-42 or Aβx-42/Aβx-40 in the subject's blood are below the standard values, or if the desmosterol level and Aβx-42 or Aβx-42/Aβx-40 and the level of helsolin in the subject's blood are below the standard values, Alzheimer's disease or a mild cognitive impairment are diagnosed. |
|
|
Analysis for quantitative determination of clostridial neurotoxin Invention relates to the field of biochemistry, namely to a method of determining an unknown concentration of a clostridial neurotoxin in the first sample with respect to a known concentration of the clostridial neurotoxin in the second sample and to a method for determining the relative activity of the clostridial neurotoxin in the first sample relative to the activity of the clostridial neurotoxin in the second sample, where the method includes the following stages: (a) contact of a cell culture with the said second sample, containing the clostridial neurotoxin in the known concentration; (c) measurement of the second effect, caused in the said cell culture by the said clostridial neurotoxin in the known concentration; (d) repetition of stages (a)-(c) with different concentrations of the said clostridial neurotoxin; (e) registration of the measured second effect of stage (d) depending on the concentration of the clostridial neurotoxin with the registration, in such a way, of the second set of data; (f) contact of the cell culture with the said first sample, containing the said clostridial neurotoxin in the unknown concentration; (h) measurement of the first effect, caused in the said cell culture by the said clostridial neurotoxin in the unknown concentration; (k) determination of the concentration of the clostridial neurotoxin, at which the said first and the said second effects are identical; and (l) comparison of the concentration of the clostridial toxin, determined in (k), with the said unknown concentration of the clostridial neurotoxin; where before the said measurement at stage (c) and stage (h) and after contact at stage (a) and stage (f) the said cell structure is subjected for 0.5 to 100 h to contact with a water medium, which does not contain the clostrdial neurotoxin, and where stage (c) is performed in case the said second sample is missing, and stage (h) is performed if the said first sample is missing. Application of the method for the determination of the unknown concentration of the clostridial neurotoxin in the first sample relative to the known concentration of the clostridial neurotoxin in the second sample is described. |
|
|
That is ensured by using an enzyme immunoassay to measure patient's oral fluid biomarkers prior to or at least 30 min after meals. The above biomarker is matrix metalloproteinase 2 (MMP 2) or matrix metalloproteinase 8 (MMP 8). For a clinical reference group, MMP 2 is adopted as 1.89-2.69 ng/ml; if the observed level is 4.2-6.6 ng/ml, the low adaptation ability is diagnosed; the level of 2.9-3.8 ng/ml shows the complete functional adaptation in the patient suffering dentofacial new growths to removable appliances of prosthetic dentures. For a clinical reference group, MMP 8 is 21.65-41.85 ng/ml; if the observed level is 433.0-1,011.6 ng/ml, the low adaptation ability is diagnosed; the level of 52.8-186.3 ng/ml shows the complete functional adaptation in the patient suffering dentofacial new growths to removable appliances of prosthetic dentures. |
|
|
Early diagnostic technique for primary open-angle glaucoma Invention refers to medicine, namely to ophthalmology, and can be used for the early diagnosis of primary open-angle glaucoma. That is ensured by measuring and assessing an intraocular pressure, examining visual fields and lachrymal fluid, measuring pro-inflammatory and anti-inflammatory cytokines and additionally measuring them in blood serum. If observing an increase of blood serum IF-γ/IL-4, IL-1β/IL-10, TNF-α/IL-10 in the patients with suspected glaucoma, cytokine coefficients equal to 3.66±1.77, 1.9±0.47, 1.20±0.32 respectively in the patients suffering the initial stage of open-angle glaucoma, the relations IF-γ/IL-4, IF-γ/IL-10, IL-1β/IL-10, TNF-α/IL-10, cytokine coefficients equal to 1.85±0.44, 1.48±0.34, 2.85±0.74, 2.42±0.71 respectively, as well as an increase of lachrymal IF-γ/IL-4, IF-γ/IL-10, IL-1β/IL-10, TNF-α/IL-10 in the patients with suspected glaucoma, cytokine coefficients equal to 1.11±0.19, 1.06±0,09, 3.81±0.63, 4.04±0.36 respectively, and in the patients with the initial stage of open-angle glaucoma, an increase of IF-γ/IL-4, IF-γ/IL-10, IL-1β/IL10, cytokine coefficients equal to 1.26±0.22, 0.84±0.08, 3.98±0.61 respectively, primary open-angle glaucoma is diagnosed. |
|
|
Method of determination of fire resistance of brick columns with mortar holder Brick columns are tested without destruction by a complex of single parameters of quality, estimating the value of the actual degree of fire resistance by the loss of bearing capacity. For this purpose the geometrical sizes of brick columns with mortar holder, conditions of heating of columns, the value of the longitudinal bend coefficient, the parameters of thermal diffusion of material of brick columns and mortar of the holder, percentage of indirect reinforcing of brickwork; the value of standard loads during fire resistance test and level of tension of hazardous cross sections of brick walls. A degree of fire resistance of brick columns with a mortar holder is determined by the evidence of losses of bearing capacity. |
|
|
Method of identification of antibodies to bacterial anti-genes Anti-gene is mixed with the tested blood serum in various dilutions in microplate cavities with V-shaped bottom with subsequent visualization of results of agglutination reaction using the ultra-violet radiation source, mainly the transilluminator. The anti-gene is the fluorescent and labelled suspension of inactivated bacterial cells which is obtained by treatment of bacterial cells by 1.0% water solution of the fluorescent dye selected from the group: bromic etidium, propidium iodide, acridic orange. |
|
|
Aerosol spraying device was used for indicator solutions spraying. An indicator formulation was sprayed over the tested surface, wherein the said formulation was sprayed in the form of a monodisperse aerosol at a distance of 10-15 cm, followed by a visual inspection of the indication effect. A 0.05-0.1% ethanol solution of mixed methyl red indicator and methyl yellow indicator at a ratio of 1:1 by volume was used for the detection of strong acids. A 0.05-0.1% solution of 4-diethyl-aminobenzene in ethanol was used for the detection of weak organic acids. |
|
Diagnostic technique for cystic fibrosis Na, B and Pb concentrations are measured in infants' sampled hair by atomic emission spectrometry with an induced argon plasma and/or mass spectrometry with an induced argon plasma; those patients who have the Na and B contents by 8.5 and 3.3 times as much as a reference group and the Pb content by 1.3 times as little as the reference group, are considered to suffer from mucoviscidosis. |
|
Method of extracting urea with removal of unwanted co2 Invention relates to medicine and describes method of removing CO2 from urea-containing plasma sample, which contains the following stages: a) obtaining plasma sample, b) addition of acid to partly remove CO2, c) sample lyophilisation to additionally remove CO2 and obtain dry sample, d) re-dissolution of dried sample and neutralisation with buffer solution to pH 4-7. |
|
Method for evaluating risk of duodenal ulcer in khakass by genetic analysis Risk factors are specified - IL-8 interleukin polymorphism is determined by restriction analysis with recovering DNA from venous blood lymphocytes; Helicobacter pylori is genetically typed by PCR with recovering DNA from gastric mucosa biopsy material in the patients being native inhabitants of the Republic of Khakassia. The risk factors are assigned with numerical values, and prognostic coefficients P1, P2 are determined. If P1>P2 a lower risk is predicted, whereas P1<P2 shows a high risk of peptic ulcer disease. The presented method possesses a high prognostic significance of the positive result in evaluating the risk of duodenal ulcer in Khakass (89.7%). |
|
|
Method involves conducting a histochemical reaction on single-layer peripheral blood films by exposing successively to solutions consisting of 5% silver nitrate, washing in 5% sodium thiosulphate and distilled water followed by finish dyeing 1% safranin in 96% ethanol, differentiating in 96% ethanol, de-watering in ascending alcohols, clearing in acid and embalming. The calcium content is studied in single erythrocytes by computed cytophotometry and stated. If cytomegaloviral infection appears to aggravate to an antibody titre of 1:1600 alongside with a decrease in calcium in erythrocyte membranes to 12.80±0.40 standard units as compared to a reference of 34.00±0.07 standard units, an increase in degenerative erythrocytes to 12.0±0.95% and a decrease in the oxyhaemoglobin content to 92.3±1.5%, a risk of hypoxia of a pregnant woman's body is stated. |
|
Peptoid ligands for autoimmune t-cell recovery and processing Group of inventions refers to molecular biology, immunology and medicine and aims at identifying populations of autologous responding T-cells in the individuals suffering autoimmune diseases. Sampling the autoimmune T-cells in the individual suffering autoimmune diseases is ensured by producing a peptoid, which specifically binds to the autoimmune T-cells. The above peptoid binds to a carrier. The sample taken from the above individual contacts the above peptoid bound to the carrier over a period of time sufficient to bind the autoimmune T-cells to the above peptoid bound to the carrier. The above carrier is then separated from the above sample. The other embodiments provide methods for destructing the autoimmune T-cells taken from the individuals suffering an autoimmune disease. |
|
Buckwheat groats freshness determination method Buckwheat groats sample is collected and boiled in water at a ratio of 1:3 during 15-20 min for intensification of aroma and then cooled to 20-25°C. Then one places five samples, 5 g each, into five vials and puts them into the automatic sampling device of a VOCmeter type multisensor system for identification of components of gas mixtures. The samples are heated to 50-55°C during 10-20 min with volatile substances removed from the containers and passed through four non-selective metal oxide sensors reacting to the sample volatile components with change of the sensitive layer electric conductivity that is converted into electric signal. The signal is registered by a PC, is processed and compared to reference samples by the principal components method. A report is retrieved in the form of a diagram by which one determines the coordinates of the gravity centre of the cluster of five points corresponding to the cluster centre along the principal components axis with buckwheat groats freshness established. Buckwheat groats are regarded fresh if the cluster gravity centre is not in excess of 45000 conventional units. |
|
Method for producing immune stimulant Method for producing an immune stimulant from cephalopods nerve tissue, marine mammal and fish brain, involving defrosting and grinding the raw material; thereafter, the ground raw material is processed in protein-degrading enzymes; the produced supernatant is separated, and the end product is dried in the specific environment. |
|
|
Method of evaluating efficiency of dialysis-filtration purification of blood Invention relates to medicine and describes method of evaluating efficiency of dialysis-filtration purification of blood, which consists in the fact that total concentration of phenylacetic (PAC), para-hydroxyphenylacetic (p-HPAC), phenyllactic (PLA) and para-hydroxyphenyllactic (p-HPLA) acids is determined in patient's blood serum before and after blood purification, and if it reduces 2 and more times, blood purification is considered effective. |
|
|
Group of inventions relates to control (monitoring) of content of mechanical impurities in flows of fluid media. The method to control content of mechanical impurities in working fluids, in particular, in liquid hydrocarbon fuel, consists in the fact that the flow of fuel is sent, maintaining the permanent flow, via a system of filtering partitions with serially reducing pore size, at the same time pressure is measured in front of each filtering partition, as well as pressure behind it, hydraulic resistance of the filtering partition in time is calculated on the basis of variation of pressure difference, then, using the produced data, they determine the degree of filtering partition clogging by comparison with available calibration data, showing variation of hydraulic resistance of the filtering partition depending on content of mechanical impurities, and on the basis of this data they determine quantity of mechanical impurities of certain size in fuel. Also the device and system for method realisation are described. |
|
Method of predicting fast virological response in patients with chronic hepatitis c Invention relates to field of medicine, in particular to hepatology, and can be used for prediction of fast virological response to therapy with pegylated interferon α and ribavirin in patients with chronic hepatitis C. For this purpose allele version of IL28B gene is identified and immunological assay is carried out. In case of presence of allele rs12979860CC/rs8099917TT immunoprediction coefficient 1 (IPC-1) is calculated by formula: IPC-1=2.774-0.833*[CD3+]-0.016*[CD4+]-0.063*[CD8+]-0.525*[CD19+], where [CD3+] is absolute content of CD3+ cells in 1 l of blood, [CD4+] is absolute content of CD3+/CD4+ cells in 1 l of blood, [CD8+] is absolute content of CD3+/CD8+ cells in 1 l of blood, [CD19+] is absolute content of CD19+ cells in 1 l of blood. If IPC-1 value is ≤1,5 development of fast virological response to standard therapy is predicted, in case of IPC-1>1.5 - its absence. If any other allele version is present IPC-2 is calculated by formula: IPC-2=3.994+0.014*[CD56+/ki-67+]-0.408*[CD25hi]-0.69*[CD25hi/FoxP3+], where [CD56+/ki-67+] is % of CD16+/CD56+/ki-67+ cells among blood lymphocytes, [CD25hi] is % of CD3+/CD25high cells among blood lymphocytes, [CD25hi/FoxP3+] is % of CD3+/CD25high/FoxP3+ cells among blood lymphocytes. If IPC-2 value is ≤3.0, fast development of fast virological response to standard therapy is predicted, in case of IPC-2 value >3.0 - its absence. |
|
Invention relates to veterinary and can be applied for reduction of toxic effect of enrofloxacin on opsonophagocytic activity of neutrophils with application of homeopathic preparations in vitro. For this purpose 0.15 ml of antibacterial preparation enrofloxacin 5% are added to leukocyte suspension in volume 0,5 ml. After that obtained solution is placed into thermostat for 3 hours at T=37±0.5°C, after incubation 0.5 ml of homeopathic preparation, diluted in physiological solution in selected dilution C6, C12, C30, C200. After that test-tubes are placed back into thermostat for 4 hours at T=37±0.5°C, after which suspension of microorganisms of reference strain Staphylococcus albus No 182 is introduced into each test-tube in dose 0.5 ml, containing 108 CFU in 1 ml of physiological solution, with the following placement into thermostat for 30 min at T=37±0.5°C, after incubation blood smears are fixed in alcohol-formalin 1:9, 40% solution of formalin and 96° alcohol, for 10 minutes and Romanowsky-Giemsa stained for 30 minutes. |
|
Method for immunohistochemical colouring of whole amounts of biological tissue samples (versions) Group of inventions relates to experimental biology and medicine for preparing whole amounts of biological tissues for optical projection tomography, confocal, multi-photonic and plane optical microscopy and can be used for preclinical tests of pharmacological preparations and evaluation of physiological effects on the body, as well as for working with biopsy material for diagnostic and research purposes. A method for immunohistochemical colouring of whole amounts of biological tissue samples includes fixing tissue with paraformaldehyde solution. The method also includes postfixation, which is first carried out with 0.5-1% paraformaldehyde for 48 hours at 4°C, and then after washing three times with a phosphate buffer at room temperature, in a solution of 20% dimethylsulphoxide in 100% methanol for 1-2 hours at room temperature. The tissue sample is then bleached in a solution with the ratio 100% methanol:dimethylsulphoxide:30% H2O2=4:1:1, for 2-4 hours in bright light at room temperature until complete discolouration. The sample is then washed 3 times for 60 minutes each in 100% methanol at room temperature and frozen for at least 1 hour at -70…-80°C, and then rehydrated successively in 50, 25 and 12.5% methanol solutions in a phosphate buffer for 45-60 minutes each at room temperature. The samples are then washed three times from methanol with phosphate buffer, two times for 1-2 hours each at room temperature, and then the last time for one night at 4°C. Subsequent permeabilisation of the sample is carried out in phosphate buffer containing 2% Triton X-100, 5% normal serum, 5% dimethylsulphoxide, for 2 hours at room temperature. The sample is then washed three times in phosphate buffer containing 0.2% Triton X-100, twice for 30-60 minutes each at room temperature and the last time for one night at 4°C. The sample then undergoes incubation successively in primary and secondary antibody solutions prepared on a buffer which consists of 0.05 M Tris-HCl with pH 7.5; 0.15 M NaCl; 0.2% Triton X-100, 5% dimethylsulphoxide and 2.5-5.0% normal serum of the host of the secondary antibodies, at 4°C for 2-7 days, while washing after each incubation in a buffer consisting of 0.05 M Tris-HCl with pH 7.5; 0.15 M NaCl; 0.2% Triton X-100, first 3 times of 60 minutes each at room temperature, and then for one night at 4°C. The coloured samples are dehydrated successively in 50, 96% ethanol solutions in phosphate buffer for 45-60 minutes each at room temperature. The samples are then held first in 100% ethanol at room temperature 2-3 times for 45-60 minutes each, and then in 2-butoxyethanol at 4°C for 12-18 hours. The dehydrated samples are brightened at room temperature in a mixture of benzyl benzoate and benzyl alcohol, taken in ratio of 2:1, for 3-4 hours. The colouring method, starting from the postfixation procedure, is carried out while mixing at a rate of 650-800 rpm. A second version of the method includes carrying out incubation in a solution of fluorescent labelled primary antibodies, which are prepared on a buffer consisting of 0.05 M Tris-HCl with pH 7.5; 0.15 M NaCl; 0.2% Triton X-100, 5% dimethylsulphoxide and 2.5-5.0% normal serum, at 4°C for 2-7 days. |
|
Total native DNA is recovered from skin and hair samples; that is followed by carrying out a polymerase chain reaction and amplification of 5.8S rRNA gene fragment of Trichophyton mentagrophytes with using specific primers. If the electrophoresis procedure enables an amplification product of 182 base pairs in size being detected, the agent is considered to be found. |
|
|
Method of life-time diagnostics of tuberculosis Invention relates to an immunological diagnostics of diseases of cattle (cattle) in the common complex of antituberculous measures. The life-time diagnostics of tuberculosis is carried out, which comprises the determining of induced luminal-dependent chemiluminescence in serum from cattle reacting positively to PPD-tuberculin. The main inductor is used as inactivated BCG vaccine. Carrying out the diagnostics enables to eliminate non-specific reactions to PPD-tuberculin in cattle in tuberculosis-satisfactory estations. |
|
|
Invention relates to physics of contact interaction of material medium, particularly, to determination of bearing capacity and stability of disperse medium under load brought about by flat stiff die. Claimed process comprises the steps that follow. Structure material medium characteristics are defined, i.e. internal friction angle φ = φstr, specific adhesion - c = cstr, specific weight - γ = γstr. At medium testing by static load process mean external pressure corresponding to mean initial (first) critical pressure applied by flat stiff B-wide die is calculated. Material medium bulk is considered to be a linearly deformed half-space. Mean barometric pressure is considered equal to pbar = 1.033 kg/cm2. Under barometric pressure, minimum initial (first) critical medium compression pressure is taken equal to where pg = (γstrh-cstr)ctgφstr is gravitational (overburden) pressure at depth h of medium mass. Note here that mean initial (first) critical medium pressure (at compression under foot and expansion beyond its edges) is defined by specified relationship. |
|
Compositions and methods for diagnostics and treatment of tumour Invention relates to field of biotechnology, in particular to isolated antibody, which specifically binds with TAT211, or its functional fragment. Also disclosed are: conjugate, which contains claimed antibody and which is specifically binds TAT211, nucleic acid, which codes claimed antibody, and cell, which produces claimed antibody. Disclosed are: method of antibody identification, method of inhibiting growth of cell, which expresses polypeptide TAT211, method of therapeutic treatment of mammal with cancer tumour, containing cells, which express polypeptide TAT211, method of detecting presence of TAT211 protein in sample and method of diagnostics of tumour presence in mammal. |
|
After splenectomy, spleen is weighed; a CD8+ T-lymphocyte count is measured morphometrically in histologic sections of a lymphoid organ after immune histochemical staining. A weight is calculated by formula: M = m × D/100%, wherein M is the CD8+ T-lymphocyte weight (g), m is the spleen weight (g), D is the CD8+ T-lymphocyte count, %. If the CD8+ T-lymphocyte value is ≥9.75 g, the refractory clinical course of immune thrombocytopenia is predicted, whereas the value of <9.75 g enables stating the form of post-splenectomy immune thrombocytopenia with the favourable clinical course. |
|
|
Method of assessment of toxicity of pollutants of waters of azov-black sea basin In this method the test objects are maintained in the test solutions; the physiological response is registered, and the toxicity level of the pollutants is determined based on toxicological parameters. The novelty is the use the test objects as round goby in the early stages of ontogeny, which spawn fertilised in vivo is collected in natural water reservoir. |
|
Method includes taking an oil sample from an engine, application of an oil drop onto a sample substrate from a filtering material, analysis of a grease path pattern, identification of specific features of the grease path pattern with the separation of the grease path pattern by colouring into three control areas. The grease path pattern is compared to reference characteristics and on the basis of this comparison they determine a level of oil contamination with mechanical admixtures, oil oxidation degree, availability of water in oil, saturation of oil with surfactants, oil an alkaline number. During the determination of the alkaline number of the investigated oil they analyse the accuracy of detection of the filtering material pattern - "water signs", and they compare the accuracy of the filtering material structure pattern within the limits of external and medium areas of the grease path of the investigated oil with the similar pattern of the reference sample, and if the pattern of the structure of the filtering material is clearly seen, then the investigated oil is admitted for further operation, and if the figure of the filtering paper structure is seen weakly or is absent, then the oil is not admitted for further operation. |
|
Basophil count is measured in a blood smear, whereas a nasal swab is analyse to determine an eosinophil count. If the blood basophil count increases more than 3 in the visual field with the nasal eosinophil count - more than 1 in the visual field in the workers employed in the harmful working conditions characterised by the high chemical factor, the harmful chemical exposure is diagnosed. Using the inventions provides higher accuracy and reliability of the chemical exposure on the chemical workers and can be used as a predictor of occupational diseases in the chemical workers. |
|
Biological material is sampled from the patient by smearing a lateral wall of a vaginal mucosa behind a hymen, and a real-time PRC is conducted to analyse a qualitative and quantitative microbial count and a CD45 gene mRNA expression level. A bacterial inflammatory process in vulva and vagina of girls aged 0 to 8 years is diagnosed if genome equivalents appear to fall within the preset range per a microbial sample, whereas CD45 gene mRNA makes min. 0.5 units in relation to reference genes. |
|
|
Immunohistochemical analysis of the tumour tissue is carried out. The level of expression is estimated for the obtained parameters. The value of regression equation is calculated by formula: Y=(5.38-16.2X1-11.2X2+5.98X3+12.4X4-5.39X5), where X1 is the condition of the regional lymphatic apparatus (0 - absence of the metastatic affection of lymph nodes, 1 - affection of up to 4 lymph nodes, 2 - affection of 4-9 lymph nodes, 3 - affection of 10 and more lymph nodes); X2 is the level of EGFR1 expression ( 1 - lower than 10%, 2 - 10% and higher); X3 is the level of thymidylate phosphorylase expression in the tumour cell cytoplasm (1 - lower than 20%, 2 - 20% and higher); X4 is the level of Ki-67 expression (1 - lower than 20%, 2 - 20% and higher); X5 is the level of VEGFR-2 expression (1 - lower than 70%, 2 - 70% and higher). The value of the probability of achieving CMR - P is calculated by formula: P=eY/(1+eY), where e is the mathematical constant, equal 2.72, and if P<0.5, a low and if P>0.5, a high probability of achieving CMR in CAX-treated patients is predicted. |
|
Kinetic method of determining biomaterial antioxidant activity Invention relates to medicine, biology, pharmacy and food technology in application to research of homolytic properties of lipids and their participation in free-radical oxidation reactions, as well as to realisation of selection of antioxidants. Method is realised by extraction from biomaterial charge of fat-soluble antioxidant in form of heptane extract and water-soluble antioxidant in form of isopropyl extract, saturation of model substrate sample of each antioxidant with oxygen with mixing at temperature 60.0±0.2°C and determination of volume of absorbed oxygen in time by volumetric method in thermostated Warburg type installation with building graph in ΔV/t coordinates, further determination of induction period value (τi) from kinetic curves and calculation of the total antioxidant activity of biomaterial components in composition of model substrate taking into account control samples, which do not contain heptanes and isopropyl extract, with model substrate of fat-soluble antioxidant including biomaterial, methyl or ethyl ether of higher unsaturated fatty acids, solution of azo-bis-isobutyronitrile (AIBN) in chlorobenzene in concentration in sample (2-60)×10-3 M; obtained sample is brought to 2 ml with chlorobenzene; model substrate of water-soluble antioxidant includes biomaterial, methyl or ethyl ether of unsaturated fatty acids, water solution of copper (II) chloride in concentration in sample (1-3)×10-3 M, water solution of cetyltrimethylammonium bromide (CTMAB) in concentration in sample (1-5)×10-3 M; obtained sample is brought to 4 ml with water, with determination of the total antioxidant activity of fat-soluble and water-soluble components of biomaterial being realised on the basis of specified calculated dependence. |
|
Method for determining ccr5 delta 32 allele polymorphism DNA is recovered from cryopreserved umbilical blood. A polymerase chain reaction (PCR) is conducted with using specific primers. The presence of specific DNA fragments is stated after electrophoretic separation of the PCR amplified mixture and ethydium bromide staining. A normal gene version is accompanied by the PCR fragment length making 224 base pairs. In CCR5 delta 32 allele polymorphism, the PCR fragment length is 192 base pairs. |
|
Method for prediction of early hypogalactia Method involves a comparative assessment of crystallographic morphology of breast secretion taken as soon as an act of delivery starts, and on the second postpartum day. If the crystallographic morphology of breast secretion remains unchanged on the first and second postpartum days as compared to that at the start of the act of delivery, an absolute probability of early hypogalactia is predicted. If the crystallographic morphology of breast secretion remains unchanged on the first postpartum day, however changes on the second postpartum day as compared to one at the start of the act of delivery, a probability of early hypogalactia is 70.3%; changes in the crystallographic morphology of breast secretion on the first postpartum day as compared to the start of the act of delivery testifies to physiological colostrum production. |
|
Method includes the determination of the seromucoid concentration in a supernatant part of the biological fluid of the nasopharyngeal aspirate. If values of the seromucoid concentration constitute 0.135-0.195 units of the optic density, a conclusion about the absence of inflammatory diseases of the brain and its meninges in term newborns with the cytomegalovirus-herpes infection is made. If the concentration of seromucoid is 0.196-0.256 units of the optic density, inflammatory diseases of the brain and its meninges in the term newborns with the intramegalovirus-herpes infection are diagnosed. |
|
Invention relates to medicine, namely to method of predicting syndrome of intrauterine foetal development retardation in third trimester of gestation in case of reactivation of chronic cytomegaloviral infection (CMI) in women with threat of miscarriage in second trimester of pregnancy. Essence of method consists in the following: in second trimester of gestation in case of reactivation of chronic cytomegaloviral infection in women, level of IgM antibodies to CMV and IgG antibodies to CMV is determined in paired blood serums, in points - A, degree of abortion threat expression is estimated in points - B, concentration of tumour necrosis factor-α (pg/ml) is determined in points C. Value of discriminant function D with boundary value equal +18.61 is calculated. If D is lower than boundary value of discriminant function, absence of syndrome of intrauterine foetal development retardation is predicted, and if D is equal or is larger than boundary value of discriminant function, development of syndrome of intrauterine foetal development retardation is predicted. |
|
|
Diagnostic technique for cirrhotic stage of chronic viral hepatitis c Invention can be used for carrying out the non-invasive diagnosis of the cirrhotic stage of chronic viral hepatitis C (CHC). The peripheral blood of a patient suffering from chronic viral hepatitis C is analysed to measure an absolute T-killer cell count with phenotype CD3+CD8+, and if the value is 295/l or less, the cirrhotic stage is diagnosed (F IV according to METAVIR). |
|
Rice groats freshness determination method Groats sample is collected and boiled in water at a ratio of 1:3 during 15-20 min for intensification of aroma and then cooled to 20-25°C. Then one collects five samples, 5 g each, into five vials and places them into the automatic sampling device. Analysis is performed using a VOCmeter type multisensor system for identification of components of gas mixtures. The samples are heated to 50-55°C during 10-20 min with volatile substances passed through the four non-selective metal oxide sensors. The electrical conductivity of the sensors sensitive layer changes in the presence of the ample volatile components, converted to an electrical signal. Then the signal is computer-processed and compared to reference samples data by the principal components method. The report is retrieved in the form of a diagram by which one determines the coordinates of the gravity centre of the cluster of five points corresponding to the cluster centre along the principal components axis. Rice groats are regarded fresh if the cluster gravity centre is not in excess of 25000 conventional units. |
|
Diagnostic technique for schizophrenia Technique involves a genetic analysis of the Vall58Met polymorphism of catechol-O-methyl-transferase gene; pre-stimulation suppression and a baseline latent period of acoustic startle are selectively assessed in Met/Met haplotype carriers. If the pre-stimulation suppression tends to decrease with increasing the baseline latent period of acoustic startle, schizophrenia is diagnosed. |
Another patent 2551113.