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Nutrient medium for detection of necrobacillosis pathogen Nutrient medium for detection of the necrobacillosis pathogen comprises medium 199, blood serum of cattle, and 40% glucose solution at a predetermined ratio of components. |
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Method is proposed to detect availability of bacteria Escherichia coli of strain O157:H7 in biological fluids, environment and food, including disintegration of bacteria with fermentative treatment and lysis by a non-ionic detergent, adsorption on paramagnetic particles with the help of a specific monoclonal antibody, detection of antigen of bacteria E.coli O157:H7 with the help of a specific biotin-modified antibody, coupling of the produced complex with non-covalent conjugate of DNA-matrix with neutravidine, dissociation of solid-phase complex "antibody-antigen E.coli O157:H7 - biotin-modified antibody-conjugate" with addition of solution of glycine - HCl pH 2.6, PCR-amplification of DNA-matrix and detection of availability of bacteria E.coli O157:H7 by speed of fluorescent signal increase. |
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Mycoplasma (mycoplasma hominis) inhibitor producer Invention refers to biotechnology, microbiology. The strain Trichoderma harzianum Rifai VKPM (Russian National Collection of Industrial Microorganisms) F-180 is applicable as Mycoplasma hominis inhibitor producer for treating mycoplasma infections. |
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Method of cultivation of biomass with increased content of lipids Method provides for implementation of cultivation stages of the biomass of microalgae on the culture medium for 8 days, and creation of stress conditions for 3 days. At that addition of potassium nitrate in specified quantity to the culture medium is performed on 1st and 4th day of cultivation of biomass of microalgae. |
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Bacterial strain komagataeibacter xylinus - bacterial cellulose producer Invention refers to biotechnology. The strain Komagataeibacter xylinus is deposited in Russian National Collection of Industrial Microorganisms (VKPM) under VKPM registration No. B-12068. Bacterial cellulose is applicable in plastic surgery, tissue regeneration and skin repair. |
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Strain of legume bacteria bradyrhizobium japonicum for production of bacterial fertiliser for soy Strain of legume bacteria Bradyrhizobium japonicum VKM B-2629D for production of bacterial fertiliser for soy is offered. |
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Biological preparation for decontamination of soil and slimes from oil and oil products Biological preparation contains the hydrolyzate containing a product of anaerobic fermentation of mix of leaves of plants, beet or reed molasses and water taken in the pre-set ratios, and the cultural liquid formed during of obtaining of microbic mass of native carbon-oxidising microorganisms at the pre-set ratio of components. |
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Strain of staphylococcus aureus bacteria used as test-culture to select antibacterial agent Strain of Staphylococcus aureus 113 bacteria having anti-lysozyme activity is deposited in state collection of microorganism and normal flora of FBIS MNIIEM named after G.N. Gabrichevsky of Rospotrebnadzor under registration No. 1253, and can be used to select the antibacterial agents suitable for effective struggle with long-lasting pathogen staphylococcus. |
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Streptococcus salivarius strain vkpm v-11174, obtained on available medium Strain Streptococcus salivarius M-11 with high antagonistic activity is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-11174 and can be used for production of fermented milk products. |
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Method of production of bacterial concentrate Method provides for preparation of the culture medium based on clarified caseous serum, sterilisation and cooling. After cooling to the culture medium 3-5% of buckthorn oil and activated culture of propionate bacterium Propionibacterium freudenreichii "Ш85" are added. Cells growth, bacterial mass separation from culture fluid are performed with suspension production. The produced suspension is developed, sealed and cooled. |
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Strain Eremothecium ashbyi Guill. 503-ssa-II is a mutant, which was selected from the strain population Eremothecium ashbyi RNCIM F-36 (NRRL Y-1363) and deposited in the RNCM under the number RNCM F-4565D. |
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Method for manufacture of food protein product enriched with fungus protein One proposes a method for manufacture of a food product of husked sunflower seeds. The method involves seeds boiling, inoculation with mycelium of at least one fungus chosen from Pleurotus ostreatus, Rhizopus oligosporus and Actinomucor elegans. Inoculation is performed by way of fungus introduction into seeds in an amount of 1-4% of the seeds weight. Then one performs fungus mycelium incubation and mycelium thermal inactivation by way of treatment with unsaturated steam. |
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Fungal strain eremothecium ashbyi - producer of essential oil similar to rose "otto" oil Strain Eremothecium ashbyi Guill. 810-ssb-III is a mutant, it was selected from the strain population Eremothecium ashbyi RNCIM F-36 (NRRL Y-1363) and deposited in the RNCM under the number RNCM F-4566D. |
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Preparation for selective apoptotic elimination of tumour cells Invention relates to microbiological industry and can be used in biotechnology, genetic engineering, medicine and veterinary. Claimed is application of RNAase Bacillus sp. VKPM B-9862 as preparation for selective apoptotic elimination of tumour cells. |
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Authentication method of dairy products Group of inventions relates to biotechnology. A method for identifying the presence or absence of a lactic acid bacterial strain comprising an IS element in a dairy product. The method comprises obtaining a nucleic sample from a dairy product, providing a primer pair specific for a region of said uniquely located IS element and a region of a nucleic acid sequence adjacent to said uniquely located IS element, performing a PCR amplification reaction with said primer pair, identifying the presence or absence of a lactic acid bacterial strain by detecting the presence or absence of said amplification product. The lactic acid bacterial strain is the Pediococcus acidilactii under accession DSM 22981 and a primer pair comprising a first primer of SEQ ID NO: 1 and a second primer of SEQ ID NO: 2 and/or a lactic acid bacterial strain Lactobacillus delbrueckii subsp.lactis under accession No DSM 24025, and a primer pair comprising a sixth primer of SEQ ID NO: 6 and a seventh primer of SEQ ID NO: 7. The said lactic acid bacterial strains may be used for the marking of dairy products. Proposed are new bacterial strains, their use in the production of dairy products as well as the dairy products containing these bacterial strains. Proposed is a kit for the specific detection of a lactic acid bacterial strain, comprising a said primer pair. |
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Method of determination of antilysozymic activity of staphylococcus Studied culture of staphylococcus is grown under continuous jogging, for check mixture of broth and culture without lysozyme is used, double measurement of the optical density of test and check cavities is performed after 2 and 4 hours of incubation, and level of antilysozymic activity is estimated as per degree of sign evidence at definite stock of staphylococcus calculated as per equation: , where K is coefficient of antilysozymic activity of staphylococcus; VC is volume of broth culture of studied stock; VL is volume of lysozyme solution of initial concentration; CL is lysozyme concentration; MO4 is average value of test cavities with the broth culture of the studied stock with lysozyme after 4 hours of incubation, units of optical density; MO2 is average optical density of test cavities with the broth culture of the studied stock with lysozyme after 2 hours of incubation, units of optical density; MC4 is average optical density of check cavities with the broth culture of the studied stock without lysozyme after 4 hours of incubation, units of optical density; MC2 is average optical density of check cavities with the broth culture of the studied stock without lysozyme after 2 hours of incubation, units of optical density. At K<0.49 low degree of evidence of antilysozymic activity is identified, at K in range 0.5-2.49 - medium degree of evidence of antilysozymic activity, K>2.5 - corresponds to high degree of evidence of antilysozymic activity of staphylococcus. |
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Streptococcus thermophilus strain used for production of fermented milk products Strain Streptococcus salivarius Bo4-I with high antagonistic activity is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-10893 and can be used for production of fermented milk products and pro-biotic preparations. |
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Strain Lactobacillus plantarum B2-II with high antagonistic activity is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-10816 and can be used for production of fermented milk products and pro-biotic preparations. |
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Strain Streptococcus salivarius M-9 with high antagonistic activity is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-11177 and can be used for production of fermented milk products and pro-biotic preparations. |
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Enterococcus strain hirae vkpm v-11173 used for obtaining of fermented milk products Strain Enterococcus hirae O-45 with high antagonistic activity is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-11173 and can be used for production of fermented milk products. |
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Strain enterococcus durans used to produce sour-milk products and probiotic specimens Strain Enterococcus durans C-45 having high antagonist activity is deposited in All-Russia Collection of Industrial Microorganisms under registration No. VKPM B-11171, and can be used during production of sour-milk products and probiotic specimens. |
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Invention relates to field of biotechnology. Claimed is strain Micrococcus sp. VKM Ac-2632D, intended for reduction of content of total nitrogen, ammonium-ion, iron and aluminium in sewage water of treatment facilities of industrial enterprises. |
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Method of production of biomass containing plantaricyne and its use for medical purposes Strain of lactobacillus Lactobacillus plantarum DSM 23213 and strain of lactobacillus Lactobacillus rossiae DSM 23214 are suggested, their joint cultivation activates synthesis of plantaricyne A, K and N and production of the product containing plantaricyne A, K and N. Product is produced by cultivation of the said strains, co-inoculation by water suspension of substrate lactobacillus, incubation at 30-37°C, preferably 30°C for 18-24 h, preferable 18 h. Said suspension has cell density about 9.0 log CFU/ml for each type of lactobacillus, and is added to the substrate in percentage ratio to volume of substrate from 1 to 4%. The produced product is used as part of pharmaceutical and cosmetic compositions, and during manufacturing of the medicament to increase barrier function of the epidermis or intestine, or for wound repair. |
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Studied culture of V. cholerae is grown up on Martin's agar, the bacterial suspension 1x109 mk/ml is prepared according to the turbidity standard of departmental standard specimens of Tarasevich State Research Institute, is added into two vessels with nutrient medium with the subsequent cultivation at 37°C. According to the special procedure the parameters of extracellular and intracellular producing of cadaverine are determined. The samples are studied in the capillary electrophoresis system Backman. The results are registered by computer electroforegramms with obtaining of parameters of samples at the time of detection of cadaverine. Using the formula the amount of the produced cadaverine in the studied samples is determined. |
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Stenotrophomonas maltophilia strain is deposited in the State collection of pathogenic microorganisms and cell cultures "GKPM-Obolensk" under the registration number V-7520. |
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New fungus protease and its application Enzyme serine fungus protease with amino-acid sequence of mature enzyme is described which at least is 86% identical to the amino-acid sequence of mature Fe_RF6318 enzyme presented in the description. The following is described: the released nucleinic acid coding the named enzyme; the recombinant expression vector containing the named nucleinic acid functionally joined with signs; and the host cell for obtaining of enzyme of serine protease according to the present invention containing the named recombinant expression vector. The method of obtaining of enzyme or fermental preparation containing the named enzyme of serine protease is offered, also the method includes the stage of cultivation of the host cell according to the present invention and either the stage of restoration of protease from the cell, or the stage of release of the cell from the cultural medium and obtaining the supernatant containing protease. The fermental preparation containing the serine fungus protease described in the invention, obtained by the named method, is offered. |
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Consortium of microorganisms Exiguobacterium mexicanum VKPM V-11011 and Bacillus vallismortis VKPM V-11017 taken in the ratio 1:1 for cleaning of various types of cryosolic soils from oily wastes is offered. |
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Improved method of producing cellulase and/or hemicellulase enzymes Invention relates to biotechnology and can be used for hydrolysis of lignocellulosic biomass. A method of producing cellulase and/or hemicellulase enzymes of Trichoderma ressei includes at least one step for growth in the presence of a carbon substrate selected from glucose, xylose, lactose, residues obtained after ethanol fermentation of monomeric sugars of enzymatic hydrolysates of cellulosic biomass and/or raw extract of water-soluble pentoses and at least one step for production in the presence of an inducing substrate, wherein said inducing substrate is a mixture containing 40-65 wt % glucose or cellulose hydrolysates, 21-25 wt % lactose and 10-39 wt % xylose or solution of hemicellulose lignocellulose hydrolysate, wherein the sum of said three components is equal to 100%. |
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Group of inventions relates to biotechnology, in particular to method of obtaining interleukin-2 preparation, interleukin-2 preparation, obtained by said method, and pharmaceutical preparation, containing said interleukin-2 preparation. Saccharomyces cerevisiae strain - interleukin-2 producent is cultivated in nutrient medium for from 36 to 50 hours with aeration from 0.2 to 0.8 litres of air per minute per 100 litres of medium. Sediment of yeast cells is obtained by centrifugation or filtration. Cells are mechanically destructed for from 15 to 40 minutes. Sediment is purified from admixture yeast lipids and proteins by washing for from 15 to 40 minutes. Sediment is purified from admixture yeast lipids and proteins by washing with n-butanol. Target protein is extracted and its chromatographic purification is carried out. |
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Biopreparation for bioremediation of oil-contaminated soils for climatic conditions of far north Biopreparation is proposed for the bioremediation of oil-contaminated soils for the climatic conditions of the Far North. The biopreparation comprises a solid substrate-carrier and a consortium of hydrocarbon-oxidizing microorganisms immobilized on its surface, comprising strains of Bacillus vallismoris RNCIM B-11017, Exguobacterium mexicanum RNCIM B-11011, Serratia plymuthica VKM B-2819D, Rhodococcus sp. VKM Ac-2626D, and the mineral nutrient substrate for bacteria. |
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Strain of Trichoderma citrinoviride RNCIM F-1228 is a producer of membrane-active antibiotics-peptaibols having antifungal and antibacterial activity. The strain was obtained by selection from a strain of Trichoderma citrinoviride TV4-1. The values of the diameter of the inhibition zone of growth of pathogenic fungi Candida albicans ATCC 2091 and Aspergillus niger INA 00760 with extracts of the culture fluid of the proposed strain are respectively 15±2 mm 25±2 mm. The value of the diameter of the inhibition zone of growth of methicillin-resistant strain of Staphylococcus aureus FDA 209R with the extract of the culture fluid of the proposed strain is 35±2 mm. |
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Claimed are strain Lactobacillus plantarum DSM 23755, strain Lactobacillus plantarum DSM 23756,strain Lactobacillus fermentum DSM 23757 and strain Lactobacillus fermentum DSM 23758, used for preparation of product, based on fermented soya, which includes isoflavone-aglycones, equol and lunasin. Mixture of said four strains can also be used for preparation of said product. To obtain it said mixture of four strains of lactic bacteria are cultivated, inoculation of obtained after cultivation water suspension of lactic bacteria into soya-based substrates with further incubation at 30-37°C, preferably 30°C, for 48-96 h, preferably 96 h, is realised. Product, obtained by claimed method is intended for treatment of hair loss. |
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K5 heparosan fermentation and purification Method of producing substantially pure heparosan from E.coli K5, comprises culturing E.coli K5 in a medium with glucose as the primary carbon source, binding heparosan to a solid-phase carrier with subsequent elution and precipitating heparosan from the eluate. Said culturing includes batch culturing phase and a batch fed culturing phase. The medium used at the batch culturing step contains (per litre) about 20 g glucose, 10-300 mg thiamine, about 13.5 g KH2PO4, about 4.0 g (NH4)2HPO4, about 1.4 g MgSO4·7H2O, about 1.7 g citric acid, and about 10.0 ml solution of trace elements, the solution of trace elements substantially consists of (per 1 l 5M HCl) 10.0 g FeSO4·7H2O, 2.0 g CaCl2, 2.2 g ZnSO·7H2O, 0.5 g MnSO4·4H2O, 1.0 g CuSO4·5H2O, 0.1 g (NH4)6Mo7O24·4H2O and 0.02 g Na2B4O7·10H2O. The feed medium used at the batch fed culturing step contains (per litre): 250-1000 g glucose, 20 g MgSO4, 0.15-0.5 g thiamine and, optionally, 47 g KH2PO4. Oxygen is supplied by bubbling air with or without additional supply of oxygen. |
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Strain of bacteria exiguobacterium sp. - decomposer of crude oil and petroleum products Bacterial strain Exiguobacterium sp. ELA-6, having the ability to dispose of crude oil and petroleum products, is deposited in Federal State Institution of Science the Institute of Biochemistry and Physiology of Microorganisms of RAS under the registration number RNCM B-2813D and can be used to purify soil and water reservoirs contaminated with crude oil and petroleum products, in a wide temperature range from +4 to +30°C. |
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Invention relates to field of biotechnology. Claimed is method of obtaining protein Bgl2p - homologue of antigen-marker of causative agents of mycoses in humans and animals. Dried biomass of commercial yeasts Saccharomyces cerevisiae is soaked in culture medium for 15-30 minutes at 27-33°C. After that, cells are precipitated by centrifugation for 2-5 minutes at 1500-2500 g and at room temperature. Obtained sediment is washed under the same conditions with distilled water. Washed cells are re-suspended in distilled water and frozen at temperature from -20 to -79°C. After that, cells are defrosted at room temperature. Toluene is added to final concentration 1-5%. Obtained mixture is incubated on rocker at 120-180 rev/min and temperature 35-45°C for 45-90 hours. After that, mixture is centrifuged, sediment of cell walls, which is washed with distilled water and re-centrifuged for 0.5-5 minutes at 2000-10000 g. Obtained sediment is re-suspended in distilled water and processed by ultrasound with frequency 20-30 kilohertz with power 30-50 Wt with cooling on ice for 2-5 minutes with 30 second interval each 20-40 seconds. After that cell walls are precipitated by centrifugation at 2000-10000 g. Washings of sediment are carried out by re-suspension and following centrifugation at room temperature in solution of 0.1-1% Triton X-100 one time, in solution of 0.2-1M NaCl three times, in 1-2% solution of sucrose one time and three times with distilled water. Target product is extracted from cell walls into distilled water with heating to 70-100°C for 5-15 minutes. After that, supernatant, containing target protein, is separated by double centrifugation at 3000-10000 g for 2-10 minutes. |
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Proposed strain is reproduced in a monolayer culture of pig kidney cells (PK), passaged cultures PSGK-30, BHK-21 and IBRS-2, for 18-24 hours of incubation the virus yield in these cell cultures reaches the values of 6.63-7.5 lg TCD 50/cm3. At high multiplicity of infection (1-10 TCD/cell) it causes TCD after 5 hours, while maintaining the initial characteristics in passaging in cell cultures for 10 passages. |
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Influenza virus strain a/17/anui/2013/61 (h7n9) for production of live intranasal influenza vaccine Vaccine strain of the influenza virus, Ortomyxoviridae, genus Influenza, A/17/Anui/2013/61 (H7N9) is deposited in the State Collection of Viruses FSBI D. I. Ivanovsky Scientific Research Institute of Virology under number 2738. |
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Method of culturing listerious microbe comprises preparing the nutrient medium containing the mother solution of leaf lettuce juice, agar and phosphate buffer in a predetermined ratio. On the prepared nutrient medium Listeria monocytogenes is applied and cultured at a temperature of 20-22°C for least 24 hours. At that the mother solution of leaf lettuce juice is prepared by sequential washing it with tap water and sterile water, followed by drying at room temperature, grinding and squeezing of juice from the resulting mass. |
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Invention relates to biotechnology. Method of producing a chitin-glucan complex and polymers containing glucose, mannose and/or galactose is disclosed. The method includes fermentation of Pichia pastoris DSMZ 70887 yeast in a culture medium containing a nitrogen source, different from a yeast extract or peptone, and a carbon source. The carbon sources used can be pure glycerine, pure methanol and glycerine- or methanol-rich mixtures. During fermentation, pH is kept I the range of 5.0-7.0 by adding an alkali, ammonia or an ammonium salt. |
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Invention relates to biochemistry. disclosed is an agent in the form of a Rhodococcus wratislaviensis VKM Ac-2623D strain for cleaning soils contaminated with hexachlorobenzene, lindane, dichlorodiphenyltrichloroethane, dichlorodiphenyldichloroethane, trillate and phthalates (dibutyl phthalate, dioctyl phthalate). |
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Method of producing forage protein product on grain raw material fermentolysat Invention relates to biotechnology and can be used in production of forage protein products. Method includes preparation of grain raw material (bran, substandard grain, fodder flour) by its milling to particles with size 1-20 mcm by means of mill under pressure with shift and preparation of water suspension of grain raw material with concentration of dry substances 15-16% with ratio of grain raw material and water 1:5. Carrying out fermentolysis of grain raw material by means of liquid α-amylase and glucoamylase with formation of glucose to 10-13%. Introduction of nitrogen and phosphorus sources into obtained fermentolysat; as nitrogen and phosphorus sources used are ammonium or phosphate salts or fertilisers, including ammophos, diammophos, nitrophosphate, with obtaining nutritional medium. Introduction into nutritional medium of culture-producent, as such used is strain of yeasts Saccharomyces cerevisiae Y-3585, and continuous growing in multisectional growing apparatus with supply of nutritional medium simultaneously by several sections with further concentration of suspension on vacuum-evaporator and drying. Moisture, released at stages of vacuum-evaporation and drying, is re-used for preparation of water suspension of grain raw material. |
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Claimed is strain of Streptomyces flavogriseus, capable of producing antibiotic complex, containing hexaenoic antibiotic of mediocidin subgroup and non-polyene antibiotic of heterocyclic structure. Strain is deposited in ARCIM under number AC-1947. |
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Nutrient medium for growing microorganisms deinococcus radiodurans Nutrient medium comprises soybean milk, glucose, MgSO4×7H2O, MnCl2, FeSO4×7H2O and distilled water in a predetermined ratio of components. |
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Strain of bacteria bacillus stratosphericus intended to produce ethanol from lignocellulosic biomass Strain of bacteria Bacillus stratosphericus Sol. NK 2, having the ability to produce ethanol, is deposited in RNCM under the registration number RNCM B-11678. The strain can be used for industrial production of ethanol, as well as in food industry, medicine, agriculture for feed production. |
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Strain of bacteria bacillus stratosphericus capable to produce ethanol from lignocellulosic biomass Strain of bacteria Bacillus stratosphericus RNCIM B-11677, producing ethanol from lignocellulosic biomass, is proposed. |
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Lactobacillus plantarum strain as hypocholesterolemic agents (versions) and application thereof Group of inventions relates to composition, comprising Lactobacillus plantarum strains, and application of strains and composition. Composition with cholesterol-lowering activity contains effective quantity of, at least, strains, selected from the group strain of Lactobacillus plantarum CECT 7527, strain of Lactobacillus plantarum CECT 7528 and strain of Lactobacillus plantarum CECT 7529. Said strains possess probiotic properties, and cholesterol-lowering activity. Pharmaceutical, veterinary or food product is obtained on the basis of claimed composition. Composition is also suitable for prevention or treatment of cardio-vascular disorders, as well as for lowering cholesterol in animals, including humans. |
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Stabiliser of colour in bacterial composition Invention relates to field of biotechnology. Claimed is application of carbonic acid salt as stabiliser of colour in bacterial composition, containing bacterial cells, belonging to genus Bifidobacterium, and ascorbate. |
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Strain of bacteria kocuria sp. - destructor of crude oil and petroleum products Strain of bacteria Kocuria sp., having the ability to dispose quickly crude oil and petroleum products (diesel fuel oil, motor oil, hydraulic oil, gas condensate), deposited in the RNCM under the registration number Kocuria sp. RNCM Ac-2624D and can be used to clean soil and water reservoirs contaminated with crude oil and petroleum products, under the wide temperature range from +4 to +30°C. |
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Strain of bacteria serratia plymuthica - decomposer of crude oil and petroleum products Strain of bacteria Serratia plymuthica ELA-9 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Serratia plymuthica VKM B-2819D and can be used for purification of soils and water reservoirs contaminated with crude oil and petroleum products, in a wide temperature range from +4 to +30°C. |
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Method of producing xanthan is proposed. Culturing the bacteria culture Xanthomonas campestris RNCM B-2228 is carried out on a nutrient medium under aeration. The nutrient medium comprises a preliminary hydrolysed low-grade timber, nitrogen sources, mineral salts. Xanthan is isolated from the culture liquid by extraction with low monohydric alcohol. |
Another patent 2550876.