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Group of inventions relates to medicine and can be used for extraction, in particular oocyte, from organism of human patient or animal patient. Vaginal needle for oocyte extraction includes first tubular section, which is in hydrodynamic connection with second tubular section. First tubular section has guide end for penetration into patient's tissue, and second tubular section, which includes tracking end for hydrodynamic connection with means of fluid reception. First tubular section has external diameter, which is smaller than external diameter of second tubular section, and first tubular section has internal diameter, which is smaller than internal diameter of second tubular section. Second tubular section has length for passing vagina and reaching tissue with oocyte. First tubular section has lengthsuitable in application for penetration into said tissue for reaching oocyte, but without penetration of second tubular section into said tissue. Method of taking sample from patient's organism includes introduction of needle for oocyte extraction into area, from which sample is to be extracted, and extraction of sample from patient's organism. |
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Method of extracorporal fertilisation Invention relates to medicine, in particular to gynecology, and deals with extracorporal fertilisation. After induction of superovulation for puncture of follicle into vagina cavity introduced is heated to body temperature tubular element with ball-like tip, which has guide canal on axis for puncture-biopsy needle. By means of it respective side of posterior vaginal fornix is pressed into abdominal cavity for maximally possible distance. Ovary is moved with side of mature follicles to contact of selected mature follicle with posterior fornix wall. Follicle centre is superposed with axis of tubular element canal, follicle is fixed in said state, distance from place of puncture of vaginal mucous membrane from the side of its cavity to follicle centre is determined and needle is moved out of the canal for said distance. After that follicular fluid is aspirated. |
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It is necessary to determine the content follicle-stimulating hormone (FSH) in follicular liquid in female patients with the syndrome of "empty" follicles (SEF), insulin-like growth factor (IGF-1) and human chorionic gonadotropin (hCG) to calculate differential value (DV) by the following formula: DV=exp(IV)/1+expIV), where IV - intermediate variable calculated by the following formula: IV=1.821+1×FSH-0.18×IGF-1+0.136×hCG, Exp - exponential function and at DV being below 0.5 the development of SEF is of absolute nature and a female patient should be recommended to have donation of oocytes, at DV being above 0.5 the development of SEF is of sporadic character and a patient should be recommended to undergo repeated curative ECF cycles. The innovation enables to elaborate the method for predicting the procedure of extracorporal fertilization and embryo transfer. |
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Isolation of inner cell mass to produce human embryonic stem cell lines Claimed method includes step of immobilization of embryo having pellucid zone, trophoblast and inner cell mass on blastocyst stage; providing of opening in embryo on blastocyst stage by using laser ablation; and removing of inner cell mass through said opening. Opening is crosses through pellucid zone and trophoblast. Laser ablation is carried out using contactless diode laser. Inner cell mass, isolated from embryo on blastocyst stage is useful in production of human embryonic stem cell lines. |
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Container unit and method for fertilizing, cultivating and transferring an embryo Device has casing having an opening for introducing culturing medium, one or several ovocytes and sperm with following embryo(s) taking away using removal catheter and self-sealing closure means for selectively opening or closing the opening in container casing. The casing has basic chamber for placing culturing medium, ovocytes and sperm therein and micro-chamber for collecting and following taking out one or several embryos. The casing has means for restricting access to the micro-chamber with removal catheter or pipette. The micro-chamber is connected to the main chamber. Method involves preparing container, filling the container with culturing medium and introducing one or several ovocytes and sperm into the culturing medium, closing the opening, incubating the closed container for fertilizing and cultivating ovocytes, creating conditions favorable for produced embryo(s) migrating into the micro-chamber and taking one or several embryo(s) from the micro-chamber using catheter or pipette. |
Another patent 2551077.