Diagnostic technique for near-graft infection of joints
SUBSTANCE: invention relates to medicine, namely to traumatology and orthopedics and can be used to diagnose periprosthetic joint infection in endoprosthetics. Essence of method: conduct a microscopic examination of smear of aspirate, stained by Gram, and further measuring the concentration of D-lactate using the D-lactam test system, test sample is prepared by adding to the 25 mcl aspirate from the endoprosthetic joint 45 mcl of the enzyme mix and 80 mcl of the substrate mixta, turbidity control is obtained by adding 45 mcl of distilled water and 80 mcl of a substrate mix to 25 mcl of aspirate from the endoprosthetic joint; resulting mixtures are incubated in a thermostat at a temperature of 37 °C±1 °C for 30 minutes and measure the optical density of the mixtures on a plate reader using a 570–600 nm filter. Then optical density of each test sample is subtracted from difference in optical density of turbidity control and empty cell and for each test sample, the concentration is calculated from the calibration schedule and at a D-lactate value of 1.2 mmol/L and more diagnosed as periprosthetic joint infection.
EFFECT: highly accurate method for the diagnosis of periprosthetic joint infection has been proposed, which allows fast, within one hour, to identify the presence of infection in the endoprosthetic joint, caused even by hard-to-cultivate microorganisms, which will allow to widely use the claimed method in the departments of traumatology, orthopedics and endoprosthetics.
1 cl, 1 tbl, 1 ex
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of developing occupational hyperkeratosis. Substance of the method consists in recovering DNA from peripheral venous blood lymphocytes, genotyping TP53 gene rs1625895 polymorphism by polymerase chain reaction with restriction enzyme digest analysis. Finding G/A genotype and A allele ensured predicting a risk of developing occupational hyperkeratosis.
EFFECT: using the invention enables predicting developing occupational hyperkeratosis after the body has been exposed to occupational hazards.
2 tbl, 2 ex
SUBSTANCE: invention relates to the field of medicine, namely to a method of diagnosing gestational diabetes mellitus in pregnant women, which includes carrying out a blood test in the morning on an empty stomach with the determination of the venous plasma glucose content. The essence of the method consists in the fact that if the level of venous plasma on an empty stomach is lower than 5.1 mmole/l, detection of diabetes risk factors: obesity with an initial, pre-pregnancy, excess body weight of more or equal 30 kg/m2, or diabetes mellitus of 2 type in the nearest relatives, or impairment of carbohydrate exchange in past medical history, or glycosoria, in pregnancy terms before 24 weeks, determination of capillary blood glucose is performed by continuous monitoring for 3 days. If registered are: one or several indices of the level of glucose in venous plasma blood on an empty stomach higher or equal to 5.1 mmole/l, but lower than 7.0 mmole/l, one hour after meal, index of the level of glucose in venous plasma higher or equal to 10 mmole/l, 2 hours after meal, index of the level of glucose in venous plasma higher or equal to 8.5 mmole/l gestational diabetes mellitus is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the efficiency of diagnostics of gestational diabetes mellitus in pregnant women.
3 dwg, 2 tbl, 1 ex
SUBSTANCE: diagnostic technique for the presence and stage of hepatic fibrosis accompanying chronic viral hepatitis C consists in determining the concentrations of gamma glutamine transferase, thrombocyte growth factor-β1, tissue inhibitor of metalloproteinases-1 and MMP-9/TIMP-2 complex in blood serum; the first stage involves calculating a probability of HCV-associated hepatic fibrosis (PPHF) by formula: PPHF=(ey/(1+ey))×100%, wherein y=-13.6+3.78·X1-0.1·X2 wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TIMP-1, ey is an exponential function. If PPHF>50%, the presence of HCV-associated fibrosis changes in the liver are predicted. At the second stage, a probability of the stage of the HCV-associated hepatic fibrosis (PSHF) is specified by formula: PSHF=(ey/(1+ey))×100%, wherein y=-12+0.134317·X1+0.527778·X2-0.05·X3, wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TGF-β1; X3 is a serum value of the prognostic factor of gamma glutamine transferase. If PSHF>50%, the presence of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis accompanying chronic viral hepatitis C (F3-4 stage); PSHF<50% shows HCV-associated hepatic fibrosis of the primary stage (F0-2 stage).
EFFECT: method is easy to use, accessible for practical medical facilities, non-invasive and safe for patients, efficient and takes no time; it is effective for screening of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis.
3 ex, 3 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology, namely, to obtaining inhibitors of adhesion and/or aggregation of platelets, and can be used in medicine. A polypeptide, used as a component of a pharmaceutical composition and in sets for screening of the inhibitors of platelet adhesion or aggregation, is obtained in a recombinant way with the application of a matrix of the salivary gland cDNA of Anopheles stephensi.
EFFECT: invention makes it possible to obtain the polypeptide, possessing inhibiting activity with respect to platelet aggregation and/or inhibiting activity with respect to platelet adhesion.
10 cl, 4 dwg, 5 ex
SUBSTANCE: invention relates to medicine, namely to dentistry, and can be used in diagnostics of dental caries by method of infrared spectroscopy. For this purpose sample of patient's saliva is preliminarily dries, dry residue is crushed and suspended in vaseline oil. IR-spectroscopy is carried out in spectrum area 1200-800 cm-1 and height of peaks of absorption bands with maximums 1070, 1017, 960, 860 cm-1 is determined. After that, values of four ratios of peak height with maximum at 1070 cm-1 to peak height with maximum 1017 cm-1, peak height with maximum at 1070 cm-1 to peak height with maximum 960 cm -1, peak height with maximum at 1070 cm-1 to peak height with maximum 860 cm -1, ratio of peak height with maximum at 1017 cm-1 to peak height with maximum 860 cm-1. Dental caries is diagnosed at the following values of ratios of peak heights 1070/1017, equal to 11.64±0.14, peak heights 1070/960, equal to 1.08±0.12, peak heights 1070/860, equal to 6.40±0.41, and peak heights 1017/860, equal to 4.15±0.44.
EFFECT: invention provides self-descriptiveness of diagnostics and increases its accuracy.
SUBSTANCE: method consists in production of cryostat sections of nerve, washing them in two portions of maleate buffer, incubation in incubation medium. Incubation is carried out for 30-60 minutes and incubation medium has the following composition: acetylcholine iodide 10 mg; 0.1 M Na-maleate buffer 2 ml; 0.1 M sodium citrate 4 ml; 0.03 M copper sulphate 4 ml; 0.005M potassium ferricianide 4 ml; medium pH constitutes from 7.4 to 7.6 and temperature is from 50 to 55°C.
EFFECT: method by invention makes it possible to evaluate viability of peripheral nerves in short term, which is necessary during reconstructive-restoring and highly technological neurosurgical operations.
SUBSTANCE: functional activity of neutrophilic granulocytes is studied by means of hemiluminiscent analysis, calculated is an index of formation of active forms of oxygen (IFAFO), representing the ratio of the area under the curve of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by a live bacterial suspension of staphylococcus aureus, to the time of achieving the maximum of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by the live bacterial suspension of staphylococcus aureus. If IFAFO value is higher than 100 o.u./s, sensitisation to staphylococcus aureus in patients with allergic rhinitis is diagnosed.
EFFECT: application of the claimed method makes it possible to diagnose staphylococcal allergy in case of allergic rhinitis before beginning therapy and contributes to the optimal selection of the patients' treatment.
1 tbl, 2 ex
SUBSTANCE: invention represents a method for determining a probability of preserving the myocardium following infarction by creating an admission examination-based data array of 7 peripheral blood parameters, 11 biochemical analysis parameters and 6 parameters of standard 12-lead electrocardiogram in 200 patients with the Q-myocardial infarction and 200 patients without the myocardial infarction. The parameters are stratified with respect to 7 intervals, wherein values related to the probability of preserving the myocardium following infarction are derived by calculating a ratio of the patients without myocardial infarction to all the patients with acute coronary syndrome. The probability is evaluated in a specific patient by analysing the above parameters, searching the respective intervals and values related to the probability of preserving the myocardium in the data array. Summing up the derived values enables calculating an integrated index, which is normalised, and a dimension from 0 to 100% is reduced.
EFFECT: invention enables increasing the prediction accuracy of preserving the myocardium in the patients with acute coronary syndrome.
1 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine, namely to immunology, and can be used in laboratory diagnostics, as a test system and a method for measuring interferon alpha (IFN-α) antiviral activity in human blood serum. The test system for measuring IFN-α activity in the human blood serum comprises diploid cells, a virus-containing fluid and standard human interferon-α (IFN-α). As the diploid cells, the test system comprises cells of the characterised line of the diploid cells - human M-20 fibroblasts at 20-33 passages cultured in the medium with added 10% fibrinolytically active plasma (FAP). As the virus, the system contains the A vesicular stomatitis virus (VSV) adapted to M-20 cells, the Indiana strain; the test system additionally contains a viral dye based on two fluorochromes - acriflavine and rhodamine C. The group of inventions also involves a method for measuring IFN-α activity in the human blood serum with the use of the developed system.
EFFECT: using the given inventions enables the quantitative good-reproducible measurement of IFN-α activity in the examined blood serum sampled by the luminescent microscopy of the vital preparations coloured in a fluorochrome-based vital dye.
3 cl, 4 dwg, 1 tbl, 4 ex
SUBSTANCE: invention describes a diagnostic technique for infected pancreonecrosis with establishing the indications for a surgical intervention by examining a patient, wherein acetic, propionic, butyric and isovaleric acids are measured in the blood by gas chromatography, and if the acetic acid concentration is more than 0.11 mmole/l enables stating infected pancreonecrosis, and if the concentration of any of three acids: propionic more than 0.095 mmole/l, butyric more than 0.0035 mmole/l, isovaleric acid more than 0.0003 mmole/l enables stating infected pancreonecrosis with active development of an anaerobic infection requiring one of versions of the surgical intervention.
EFFECT: invention enables providing the objective and accurate diagnosing of pancreonecrosis transformation into the stage of infection by using the qualitative parameters with no undesired side effects.
FIELD: medicine, hepatology.
SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, infectology, hepatology.
SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: clinical medicine, pulmonology.
SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.
EFFECT: higher efficiency of prediction.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.
EFFECT: higher efficiency of therapy.
1 cl, 3 dwg, 4 ex
FIELD: medicine, gastroenterology.
SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.
EFFECT: higher accuracy of prediction.
SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.
EFFECT: high accuracy of diagnosis.
4 cl, 2 tbl
FIELD: medicine, gastroenterology.
SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.
EFFECT: higher efficiency and accuracy of detection.
FIELD: medicine, gastroenterology.
SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.
EFFECT: higher efficiency of diagnostics.