Method for chronic endometritis diagnostics at medium stage of secrection phase
SUBSTANCE: invention is intended for chronic endometritis (CE) diagnostics at the medium stage of the secretion phase. Pipelle biopsy of the endometrium using an atraumatic aspiration curette is performed to take a fragment of the uterine cavity mucous membrane at the medium stage of the secretion phase. By quantitative RT-PCR in real time, the mRNA level of the VEGFA gene is determined. At a level greater than 0.250, a conclusion is made about the presence of CE.
EFFECT: effective diagnosis of chronic endometritis on the profile of genes mRNA expression in the endometrium during the implantation window in women with tubal peritoneal infertility.
2 dwg, 3 ex
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of developing occupational hyperkeratosis. Substance of the method consists in recovering DNA from peripheral venous blood lymphocytes, genotyping TP53 gene rs1625895 polymorphism by polymerase chain reaction with restriction enzyme digest analysis. Finding G/A genotype and A allele ensured predicting a risk of developing occupational hyperkeratosis.
EFFECT: using the invention enables predicting developing occupational hyperkeratosis after the body has been exposed to occupational hazards.
2 tbl, 2 ex
SUBSTANCE: invention relates to the field of medicine, namely to a method of diagnosing gestational diabetes mellitus in pregnant women, which includes carrying out a blood test in the morning on an empty stomach with the determination of the venous plasma glucose content. The essence of the method consists in the fact that if the level of venous plasma on an empty stomach is lower than 5.1 mmole/l, detection of diabetes risk factors: obesity with an initial, pre-pregnancy, excess body weight of more or equal 30 kg/m2, or diabetes mellitus of 2 type in the nearest relatives, or impairment of carbohydrate exchange in past medical history, or glycosoria, in pregnancy terms before 24 weeks, determination of capillary blood glucose is performed by continuous monitoring for 3 days. If registered are: one or several indices of the level of glucose in venous plasma blood on an empty stomach higher or equal to 5.1 mmole/l, but lower than 7.0 mmole/l, one hour after meal, index of the level of glucose in venous plasma higher or equal to 10 mmole/l, 2 hours after meal, index of the level of glucose in venous plasma higher or equal to 8.5 mmole/l gestational diabetes mellitus is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the efficiency of diagnostics of gestational diabetes mellitus in pregnant women.
3 dwg, 2 tbl, 1 ex
SUBSTANCE: diagnostic technique for the presence and stage of hepatic fibrosis accompanying chronic viral hepatitis C consists in determining the concentrations of gamma glutamine transferase, thrombocyte growth factor-β1, tissue inhibitor of metalloproteinases-1 and MMP-9/TIMP-2 complex in blood serum; the first stage involves calculating a probability of HCV-associated hepatic fibrosis (PPHF) by formula: PPHF=(ey/(1+ey))×100%, wherein y=-13.6+3.78·X1-0.1·X2 wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TIMP-1, ey is an exponential function. If PPHF>50%, the presence of HCV-associated fibrosis changes in the liver are predicted. At the second stage, a probability of the stage of the HCV-associated hepatic fibrosis (PSHF) is specified by formula: PSHF=(ey/(1+ey))×100%, wherein y=-12+0.134317·X1+0.527778·X2-0.05·X3, wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TGF-β1; X3 is a serum value of the prognostic factor of gamma glutamine transferase. If PSHF>50%, the presence of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis accompanying chronic viral hepatitis C (F3-4 stage); PSHF<50% shows HCV-associated hepatic fibrosis of the primary stage (F0-2 stage).
EFFECT: method is easy to use, accessible for practical medical facilities, non-invasive and safe for patients, efficient and takes no time; it is effective for screening of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis.
3 ex, 3 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology, namely, to obtaining inhibitors of adhesion and/or aggregation of platelets, and can be used in medicine. A polypeptide, used as a component of a pharmaceutical composition and in sets for screening of the inhibitors of platelet adhesion or aggregation, is obtained in a recombinant way with the application of a matrix of the salivary gland cDNA of Anopheles stephensi.
EFFECT: invention makes it possible to obtain the polypeptide, possessing inhibiting activity with respect to platelet aggregation and/or inhibiting activity with respect to platelet adhesion.
10 cl, 4 dwg, 5 ex
SUBSTANCE: invention relates to medicine, namely to dentistry, and can be used in diagnostics of dental caries by method of infrared spectroscopy. For this purpose sample of patient's saliva is preliminarily dries, dry residue is crushed and suspended in vaseline oil. IR-spectroscopy is carried out in spectrum area 1200-800 cm-1 and height of peaks of absorption bands with maximums 1070, 1017, 960, 860 cm-1 is determined. After that, values of four ratios of peak height with maximum at 1070 cm-1 to peak height with maximum 1017 cm-1, peak height with maximum at 1070 cm-1 to peak height with maximum 960 cm -1, peak height with maximum at 1070 cm-1 to peak height with maximum 860 cm -1, ratio of peak height with maximum at 1017 cm-1 to peak height with maximum 860 cm-1. Dental caries is diagnosed at the following values of ratios of peak heights 1070/1017, equal to 11.64±0.14, peak heights 1070/960, equal to 1.08±0.12, peak heights 1070/860, equal to 6.40±0.41, and peak heights 1017/860, equal to 4.15±0.44.
EFFECT: invention provides self-descriptiveness of diagnostics and increases its accuracy.
SUBSTANCE: method consists in production of cryostat sections of nerve, washing them in two portions of maleate buffer, incubation in incubation medium. Incubation is carried out for 30-60 minutes and incubation medium has the following composition: acetylcholine iodide 10 mg; 0.1 M Na-maleate buffer 2 ml; 0.1 M sodium citrate 4 ml; 0.03 M copper sulphate 4 ml; 0.005M potassium ferricianide 4 ml; medium pH constitutes from 7.4 to 7.6 and temperature is from 50 to 55°C.
EFFECT: method by invention makes it possible to evaluate viability of peripheral nerves in short term, which is necessary during reconstructive-restoring and highly technological neurosurgical operations.
SUBSTANCE: functional activity of neutrophilic granulocytes is studied by means of hemiluminiscent analysis, calculated is an index of formation of active forms of oxygen (IFAFO), representing the ratio of the area under the curve of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by a live bacterial suspension of staphylococcus aureus, to the time of achieving the maximum of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by the live bacterial suspension of staphylococcus aureus. If IFAFO value is higher than 100 o.u./s, sensitisation to staphylococcus aureus in patients with allergic rhinitis is diagnosed.
EFFECT: application of the claimed method makes it possible to diagnose staphylococcal allergy in case of allergic rhinitis before beginning therapy and contributes to the optimal selection of the patients' treatment.
1 tbl, 2 ex
SUBSTANCE: invention represents a method for determining a probability of preserving the myocardium following infarction by creating an admission examination-based data array of 7 peripheral blood parameters, 11 biochemical analysis parameters and 6 parameters of standard 12-lead electrocardiogram in 200 patients with the Q-myocardial infarction and 200 patients without the myocardial infarction. The parameters are stratified with respect to 7 intervals, wherein values related to the probability of preserving the myocardium following infarction are derived by calculating a ratio of the patients without myocardial infarction to all the patients with acute coronary syndrome. The probability is evaluated in a specific patient by analysing the above parameters, searching the respective intervals and values related to the probability of preserving the myocardium in the data array. Summing up the derived values enables calculating an integrated index, which is normalised, and a dimension from 0 to 100% is reduced.
EFFECT: invention enables increasing the prediction accuracy of preserving the myocardium in the patients with acute coronary syndrome.
1 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine, namely to immunology, and can be used in laboratory diagnostics, as a test system and a method for measuring interferon alpha (IFN-α) antiviral activity in human blood serum. The test system for measuring IFN-α activity in the human blood serum comprises diploid cells, a virus-containing fluid and standard human interferon-α (IFN-α). As the diploid cells, the test system comprises cells of the characterised line of the diploid cells - human M-20 fibroblasts at 20-33 passages cultured in the medium with added 10% fibrinolytically active plasma (FAP). As the virus, the system contains the A vesicular stomatitis virus (VSV) adapted to M-20 cells, the Indiana strain; the test system additionally contains a viral dye based on two fluorochromes - acriflavine and rhodamine C. The group of inventions also involves a method for measuring IFN-α activity in the human blood serum with the use of the developed system.
EFFECT: using the given inventions enables the quantitative good-reproducible measurement of IFN-α activity in the examined blood serum sampled by the luminescent microscopy of the vital preparations coloured in a fluorochrome-based vital dye.
3 cl, 4 dwg, 1 tbl, 4 ex
SUBSTANCE: invention describes a diagnostic technique for infected pancreonecrosis with establishing the indications for a surgical intervention by examining a patient, wherein acetic, propionic, butyric and isovaleric acids are measured in the blood by gas chromatography, and if the acetic acid concentration is more than 0.11 mmole/l enables stating infected pancreonecrosis, and if the concentration of any of three acids: propionic more than 0.095 mmole/l, butyric more than 0.0035 mmole/l, isovaleric acid more than 0.0003 mmole/l enables stating infected pancreonecrosis with active development of an anaerobic infection requiring one of versions of the surgical intervention.
EFFECT: invention enables providing the objective and accurate diagnosing of pancreonecrosis transformation into the stage of infection by using the qualitative parameters with no undesired side effects.
FIELD: veterinary medicine.
SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.
EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.
SUBSTANCE: invention refers to medicine and biology, namely to a method for additional electron-dense contrast enhancement of acid groups of biomolecules accompanying the histochemical detection of sodium cations in pulmonary and tracheal cell and tissue ultrastructures. Substance of the method consists in fixing tissue slices, washing the surface in bidistilled water, placing into a diluted agent containing 4% osmium tetraoxide 1 ml and 2% potassium hexahydroantimonate 8 ml. The tissue slices are stained for 4 hours while stirring strongly and washed in bidistilled water. The tissue slices are further prepared by slicing thinner and dehydrated; the semi-thin and ultrathin slices are produced and studied by a transmission electron microscope, which is followed by computer processing to detect diffuse selective staining of the acid cell ultrastructures and intracellular substance.
EFFECT: using the declared method enables the additional electron-dense contrast enhancement of the acid groups of biomolecules accompanying the histochemical detection of sodium cations in the pulmonary and tracheal cell and tissue ultrastructures.
5 dwg, 1 ex
SUBSTANCE: preparation method of a dielectric specimen for investigation on a focused-beam electronic microscope of its micro- and nanostructure involves application of a current-carrying coating onto the specimen surface and provision of electrical contact of the specimen coating with the current-carrying object table. The current-carrying coating is applied by wetting of the specimen surface with a solution of hydrophilic non-evaporable non-flammable non-toxic current-carrying ionic liquid in the form of tetrachloroferrate of N-decylpyridinium in acetone and further drying of the specimen in the air till complete removal of a volatile component.
EFFECT: prevention of accumulation of electrical charges on the surface of dielectric specimens.
FIELD: oil and gas industry.
SUBSTANCE: sampling device contains a main pipeline, a sampling section joined with the main pipeline with a possibility of sampling with coverage of liquid flow cross section, a sampling tap and a manometer. The sampling tap is designed as housing and bushing which is rigidly joined with a handle, and in a starting position the bushing shuts a drain hole of the housing, and the handle can move together with the bushing, opening the drain hole of the housing in working position. The hollow cylinder with the central channel is installed in the main pipeline, and the hollow cylinder from side of liquid flow movement is fitted with the input cone tapering the flow and the output cone expanding the flow on the other side of the hollow cylinder. The whirler fitted inside with tangential channels is installed in the central channel of the hollow cylinder from the input cone. The bushing of the sampling tap covers hermetically from outside the housing with the drain hole and can move restrictedly in axial direction with reference to the housing. The housing has the first and second external cylindrical grooves and the cutting spring lock ring is located inside. The bushing is fitted with internal ring sampler.
EFFECT: improvement of quality of liquid sampling, improvement of operational reliability of the sampling tap and increase of level of correctness of identified process parameters of wells and layers according to analyses of samplings.
SUBSTANCE: method comprises the steps: collecting from the bile ducts of liver of domestic and/or wild animals infected with fasciolas of only live adult F. hepatica. Placement them into individual tubes with filtered and centrifuged bile diluted with isotonic solution of sodium chloride 1:1. Exposure of tubes at t = 38-39°C if F. hepatica is from cattle, and t = 39-40°C if F. hepatica is from sheep and/or goats, under conditions of thermostat for 5 hours. Subsequent washing eggs in isotonic solution of sodium chloride.
EFFECT: invention enables to obtain up to 100 percent of fertilised eggs of Fasciola of species Fasciola hepatica and can be used for study in the laboratory or field experiments in solving fundamental and applied scientific tasks in the field of epizootiology, treatment and prevention of fascioliasis of domestic or wild animals.
SUBSTANCE: invention relates to the field of agricultural machinery industry. The device for sampling chopped straw from grain combine harvesters comprises collectors, a retainer, a bracket and a control lever. The collectors are arranged in rows, the number of which in the transverse direction is determined by the header coverage and the selected number of the studied areas, and in the longitudinal - replication of sampling. The collectors are interconnected in longitudinal rows by means of flexible connections equal in magnitude, so that the total length of the row does not exceed the distance to the ground mass descent area on the stubble. The first collectors in each longitudinal row have easily removed connections on the holes in the rod-float leveller which is located perpendicular to the direction of the combine movement and retained by the retainer driven by the control lever.
EFFECT: invention provides a boundary separation of the comparable areas in sampling and reduction of the probability of failure of experimental equipment.
2 cl, 1 dwg
SUBSTANCE: invention relates to the method of analysis of a set of ferromagnetic particles. The method is characterized by that the particles of the named set are levelled in such a way that each of the named particles is oriented practically in the same direction. Then the particles of the named set are fixed in this aligned direction and the internal areas of the named particles levelled in such a way are uncovered. After that the nature of the alloy comprised by each of the named particles is identified, the named particles are grouped by categories depending on their nature and the metallurgical structure and chemical composition of one or more of the named particles in each category are determined.
EFFECT: improvement of accuracy and reliability of the analysis of ferromagnetic particles.
14 cl, 6 dwg
SUBSTANCE: invention relates to forecast of ageing processes of the synthetic polymer materials (SPM) depending on duration of their operation or storage. Analysis of the volatile organic compounds (VOC) migrating from SPM is performed by active sampling for sorbent, with further thermal desorption and gas chromatographic analysis. Forecast of the ageing processes of the material and estimation of toxicity of the gas discharge are performed as per dynamic of the qualitative and quantitative composition of gas discharge components in SPM initial state, and during artificial climatic thermal-humidity ageing. Dynamics analysis of the total gas discharge (ΣT) from each material is performed for all substances migrating from studied SPMs. Change of toxicity is estimated and forecast of the material ageing is performed as per developed indices of total gas discharge (ΣT) and as per hygienic index P=(ΣTinitial/ΣTn)/V, where Tinitial and Tn are indices of toxicity of gas discharge of each substance in initial state and after ageing, respectively, and ΣTinitial and ΣTn are total indices of toxicity of gas discharge of all components of SPM in initial state and after ageing, V is duration of ageing (year, month).
EFFECT: invention ensures high accuracy of the method of VOC qualitative and quantitative composition determination in gas discharge during materials ageing and the analysis results repeatability.
FIELD: physics, acoustics.
SUBSTANCE: group of inventions relates to an apparatus for irradiating a sample with focused acoustic energy, a device which is part of said apparatus, a cartridge for said device and a method of irradiating a sample with focused acoustic energy. The apparatus comprises a device, a cartridge, a completely solid-state connector and a source for generating acoustic energy. The cartridge has a chamber for receiving a sample, and the completely solid-state connector provides a completely dry coupling of acoustic energy between the source and the cartridge. The device and the cartridge are adapted for inserting the cartridge containing a sample into the device and are separable, and the focused acoustic energy is focused high-intensity ultrasound. The device has a source for generating acoustic energy and the cartridge has a chamber for receiving a sample.
EFFECT: improved sample processing.
17 cl, 24 dwg
FIELD: motors and pumps.
SUBSTANCE: simulation device contains an oil batcher, a dispersion chamber and a lubricant oil decomposition chamber (1). At the air outlet downstream the chamber the diffuser (2) is located. On the chamber the heater (3) with the thermocouple (4) and the thermorelay (5) is installed. The device includes the air duct (6) supplying the pumped-over hot air into the lubricant oil decomposition chamber connected through the manometer (7) to the air compressor (8). The device contains the cylinder (13) filled with ultra-pure nitrogen, (23, 24) the sealed gage tank with air cavity with oil and a cover for oil filling, with the oil pipeline connected to it through the gas pipeline with the regulator (12), the adapter (11) and cap nuts. The gage tank (9) is connected through the adapter (11) with cap nuts (20, 21) to the measured capillary (15) in a cooling jacket (16) with circulating water through the thermostat with the pump (18) and radiators, attached to the decomposition chamber by means of the cap nut (22) and the sealing cone (25). Also the device comprises the additional chamber (26) screwed to the main decomposition chamber of (1) coaxially and sealed with a gasket (27), with the rod with a flywheel (17) installed inside, with threaded and non-threaded parts. Meanwhile the threaded part is implemented with a possibility of movement in the internal washer with a thread (28) for adjustment of the decomposition chamber volume and change of conditions of simulation of oil concentration, while the non-threaded part is sealed in a gland with graphite seal (29).
EFFECT: improvement of accuracy of simulation of composition of oil decomposition products in aviation gas-turbine engines.
SUBSTANCE: invention relates to field of biotechnology, immunology and genetic engineering. Genetic modification contains deletion of endogenic low-affinity FcγR locus, where contain functional FcRγ-chain. Modified mice express up to five genes of low-affinity human FcγR on antigen-presenting cells of host immune system. Also described is method of screening substance, containing Fc site of human antibody by means of said mice. Invention can be used in the field of medicine.
EFFECT: presented are genetically modified mice, not related to man, and methods and compositions for their obtaining and application.
15 cl, 11 dwg, 6 tbl, 8 ex