Method of occupational selection of persons for work on destruction of chemical warfare agents

FIELD: medicine.

SUBSTANCE: invention is intended for the occupational selection of persons for the work on the destruction of chemical warfare agents (CWA). In peripheral blood lymphocytes, the number of chromosomal aberrations is examined. The persons who pass the occupational selection are whose who did not show an increase in the number of cells with chromosomal aberrations which exceed the normal level of spontaneous chromosomal aberrations by more than 2 times.

EFFECT: increased objectivity of the occupational selection of persons for the work on the destruction of chemical warfare agents.

1 dwg, 4 tbl, 1 ex

 



 

Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to investigation and analysis of high-molecular weight materials via infrared spectroscopy when determining the composition of polyacrylate and polyacrylonitrile copolymers to control the quality of carbon fibre. The method includes measuring the infrared absorption spectrum of films of test samples of polyacrylonitrile fibre using Fourier transform infrared spectroscopy in the 3000-800 cm-1 region and determining the content of acrylonitrile and methyl acrylate from normalised spectra based on characteristic peaks thereof. During sample preparation, the number of sample preparation steps is maximally simplified and reduced and dimethyl sulphoxide which does not absorb in the operating infrared region is used. When processing the obtained infrared spectra, the method employs adjustment of the entire base line, smoothing of the shape of the peaks of the investigated compounds and decomposition of the composite peak at 1733±3 cm-1 into components.

EFFECT: invention provides a method for reproducible, precision and sensitive determination of basic components of polyacrylonitrile.

2 cl, 3 tbl, 1 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to genetic engineering and biotechnology. Disclosed is a method of evaluating bioactivity of chemical compounds, where the first step includes transient transfection of cell line HEK 293 with plasmid vector pX-Y-neo (X is any eukaryote transcription factor, Y is a proteotypic peptide corresponding to said transcription factor), which contains a minimal human adenovirus type 5 promoter; a green fluorescent protein gene; a nucleotide sequence which codes the binding site of the transcription factor; a nucleotide sequence which codes the proteotypic peptide; a neomycin resistance gene; the second step includes determining the activity of the transcription factor via fluorescent analysis and chromatographic-mass spectrometer measurement of the content of the proteotypic peptide in the transfected cell culture in the presence of the test substance compared to a transfected intact cell culture.

EFFECT: invention provides fast and highly sensitive evaluation of bioactivity of chemical compounds.

2 dwg, 1 ex

FIELD: agriculture.

SUBSTANCE: device for research of physical-mechanical properties of tuberous roots comprises a frame (1) with an electric motor (2) attached to it, on a shaft of which a removable disc (3) is mounted with the surface under study, and the guide (4) on which a movable trolley (5) is mounted. The movable trolley (5) is connected on one side to the screw mechanism (7) through the spring (6), and on the other side to a load (8) through the block (9). The device is provided with a frequency converter (13) which enables to adjust smoothly the speed of rotation of the removable disc (3), and also a screw mechanism (15) with a guide by which the gap between the trolley (5) and the removable disc (3) is implemented.

EFFECT: increase in the accuracy of the results of research on friction process of rest and movement of tuberous roots on different surfaces.

1 dwg

FIELD: measurement equipment.

SUBSTANCE: invention relates to the field of assessment of atmospheric air pollution degree and may be used in monitoring of atmospheric air in background and urbanised territory. The method provides for identification of a test site with size of 25×25 m, detection of external criteria of lichen on the test site, detection of available indicator types of lichen and frequency of their occurrence. On the basis of the produced data the lichenoindex is calculated, according to the provided classification of lichenoindex, they determine degree of atmospheric air pollution.

EFFECT: invention makes it possible to detect degree of atmospheric air pollution by lichen.

4 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: object is positioned on porous substrate, fixed to the substrate surface and scanned by probe microscopy method. Substrate with through pores of smaller size than the diameter of a study object is used, and an object is fixated by laminar flow of liquid or gas supplied to the substrate from the side of scanning, with clamping force exerted by the flow on an object within 10-12-10-3 N range.

EFFECT: possible study of structures and mechanical properties of organic and inorganic objects, enhanced information content of nano and micro object studies by probe microscopy.

7 ex

FIELD: agriculture.

SUBSTANCE: invention relates to ecology. The invention provides a method of determining the quality of the environment by EPR-spectroscopy of lichens, including the collection of samples of lichen thalli from the trunks of trees growing in an industrial and background area, which is not contaminated by anthropogenic emissions into the environment, cleaning, drying, grinding, which is characterised by the fact, that drying is carried out at a temperature of 85-95°C to constant weight and ground, the EPR spectra are removed, from which the concentration of paramagnetic centres is determined, in the excess of the concentration of the paramagnetic centres in the lichen samples collected in the industrial zone, over the concentration of the paramagnetic centres of the lichen samples from the background area the low quality of the environment in the industrial zone is detected, and in case of equal concentrations of the paramagnetic centres - the acceptable quality of the environment is detected, and in the studies the samples of the same species of lichen are used.

EFFECT: invention provides the improvement of the method of lichenoindication, improvement of the quality of evaluation of the test objects, obtaining objective result.

2 ex, 2 tbl, 3 dwg

FIELD: ecology.

SUBSTANCE: method of determining ammonium compounds in the atmosphere of livestock complexes comprises collecting samples of lichen from trees growing in the background zone, which has no emissions of pollutants into the atmosphere. The data for the samples of lichen collected in the zone of pollutants emission to the atmosphere is compared with data for laboratory standards by IR spectroscopy method. For obtaining the standards under laboratory conditions the interaction process of lichen of the background zone with emission of pollutants contributing to formation of ammonium sulphate is simulated. Lichen Parmelia sulcata is used as bioindicator.

EFFECT: invention enables to determine the level of ammonium compounds in the atmosphere of livestock complexes.

2 tbl, 1 dwg

FIELD: metallurgy.

SUBSTANCE: invention relates to ferrous metallurgy and can be used to determine chemical composition of materials containing lump metal and used as raw material in cast iron production. The method involves separation of material into metal and slag fractions, measurement of metal fraction weight, grinding of slag fraction down to the fineness of 5 mm at most and determination of weight ratio of total ferrum and of necessary components in it by complete acid digestion, calculation of weight ratio of total ferrum and of components in the material, after grinding a sample is taken with the fineness of from 0.16 mm to 5 mm at most and chemical analysis is performed.

EFFECT: improved information value and reliability of analysis.

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for the prediction of the early stage of lymphocyte apoptosis. That is ensured by isolating cells, incubating for 48 hours at temperature 37°C with 5% CO2 with adding apoptosis inductor, dexamethasone in the concentration of 10-4 mole/ml. A lymphocyte viability is quantified by trypan blue inclusion, the recovered and oxidised glutathione concentrations are measured in lymphocyte lysate after the 30-minute pre-incubation with 2-vinylpyridine 10 mM. The early stage of lymphocyte apoptosis is stated, if observing an integrated decrease of the recovered glutathione concentration by 17% and more and an increase of the oxidised glutathione concentration by 19% and more as compared to the reference.

EFFECT: using the presented method in medical practice enables predicting the antioxidant state of the patient's body accompanying various diseases as shown by the early stage of lymphocyte apoptosis evaluated.

1 tbl

FIELD: chemistry.

SUBSTANCE: method includes the selection and preparation of samples to be analysed, selection of specified volumes of solutions of a test system components, placement of the samples to be analysed and the test system components into a cuvette, registration of chemiluminiscence with further quantitative estimation of its value with taking into account the background signal of chemiluminiscence. The weight of porting of the sample of the material to be analysed is taken such that corresponds to the value of a specific surface 0.20±0.05 m2/g, and in case when it is not possible to determine the value of the specific surface of the sample to be tested, the weight of the taken portion is 0.010±0.005 g. The portion of the sample of the material to be analysed is placed into a cuvette with the further successive addition of the test system components: 0.01M solution of luminal in 0.5 NaOH solution and a solution of hydrogen peroxide of a 20-30% concentration to fill the working space in the cuvette, keeping the ratio luminal:hydrogen peroxide equal to 2:5. After that, values of chemiluminiscence are registered for 125 minutes and the total value of chemiluminiscence is calculated.

EFFECT: identification of the free-radical activity of solid materials by the method of the chemiluminiscence registration by means of the system of chemical reagents without the application of biological substrates in the test system.

3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention represents a method for assessing a probability of progression of the clinical course of oral lichen planus in the patients with hypercholesterolemia and hepatobiliary disorders.

EFFECT: invention represents developing criteria for assessing a probability of progression of the clinical course of oral lichen planus in the patients with hypercholesterolemia and hepatobiliary disorders.

4 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: technique involves performing clinical laboratory blood analysis, measuring neutrophil, lymphocyte, thrombocyte and erythrocyte acid phosphatase activity; an integral diagnostic index of lysosomal blood activity is calculated by the presented formula. If the integral diagnostic index of lysosomal blood activity exceeds 26.2 activity units, the electromagnetic body exposure is diagnosed; if the above index is less than 26.2 activity units, the negative body response to the exposure is concluded.

EFFECT: invention enables providing the more reliable diagnosis of the electromagnetic exposure by the integral evaluation of lysosomal activity in all blood cell types that is of high importance for working capacity examination and employment assistance resolution.

3 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: chronic infectious-inflammatory diseases (CIIDs) are diagnosed. Clinical blood analysis and bacteriological tests are conducted. A sensibilisation index (SI) and an immune responsiveness index (IRI) are calculated; total microbial count per 1 m3 of the working space air is measured, and the total microbial number (TMN) is derived. If the TMN is less than 500 CFU/m3 with no CIIDs diagnosed accompanied by the SI of less than 1.08 standard units and the IRI of less than 13 standard units, the immunoassay is considered to be inadvisable. If the TMN falls within the range of 500-2,500 CFU/m3 with one CIID diagnosed accompanied by the SI from 1.08 to 1.3 standard units and the IRI from 13.1 to 15.7 standard units, the immunoassay with the first-level tests seems advisable. Whereas the TMN exceeding 2,500 CFU/m3 with at least two CIIDs accompanied by the SI of 1.4-1.5 standard units and the IRI of 15.8-18.3 standard units, the immunoassay with the second-level tests is thought expedient.

EFFECT: invention enables detecting the workers in need of further examination for the purpose of timely immune correction in the setting of mass routine examinations.

1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: senior guinea pigs are exposed to optical light generated by light emitting diodes or fluorescent tubes at a colour temperature of 4,500 K within the wavelength region of 360-460 nm for various time distances. The exposure is assessed by functional activity indices of neutrophilic granulocytes and a mononuclear cell count.

EFFECT: using the method requires no expensive equipment, critical chemicals; it is of a particular importance for evaluating the biological safety of new artificial light sources embedded into a light-colour medium; it provides expanded information on biological effects of optical light.

3 tbl

FIELD: medicine.

SUBSTANCE: invention relates to laboratory diagnostics, namely to a method for the preservation of protein of urine, sampled for the diagnostics of infectious haemorrhagic fever with renal syndrome (HFRS) by PCR method. The essence of the method consists in the following: urine is sampled from a patient in an acute period of a disease, the protein is separated, precipitated by a method of salting-out with ammonium sulphate with its further drying and preservation at a temperature of 20-24°C. Diagnostics by means of PCR method is carried out after salt removal by a method of dialysis with flowing water for 24-48 hours of a mass, obtained by mixing 3-5 g of the dry protein and 8-12 ml of distilled water.

EFFECT: application of the claimed method makes it possible to realise HFRS diagnostics after many years with the application of urine protein, preserved in a dry form.

1 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: method involves measuring blood leukocytes, blood leukocyte shift index (BLSI), neutrophil/lymphocyte ratio (NLR), leukocytal intoxication index (LII). If observing BLSI >4, NLR >5, LII >4 and detecting TNFα G-308A gene mutation, an increasing intensity of a systemic inflammatory reaction is predicted in the given category of patients.

EFFECT: using the given method enables predicting a degree of the intensity of the systemic inflammatory reaction in geriatric patients suffering from acute myocardial infarction, performing primary prevention in the patients with TNFα G-308A polymorphism detected and the positive medical history of the ischemic heart disease, as well as the secondary prevention of the complicated acute myocardial infarction.

3 ex

FIELD: medicine.

SUBSTANCE: method involves analysing the newborn's clinical and functional health indicators, namely the ventilation parameters: fractional oxygen concentration in the inhaled mixture, mean airway pressure, partial oxygen pressure in the arterial postductal blood, and calculating an oxygenation index. If the oxygenation index is 3.5 or less, non-invasive pulmonary ventilation is considered to be effective, whereas the oxygenation index of more than 3.5 shows therapeutic ineffectiveness.

EFFECT: method enables predicting a success rate of various techniques for non-invasive pulmonary ventilation in premature newborns with a very low and extremely low birth body weight diagnosed with respiratory distress syndrome.

2 ex

FIELD: medicine.

SUBSTANCE: method involves analysing the newborn's clinical and functional health indicators, namely the ventilation parameters: fractional oxygen concentration in the inhaled mixture, mean airway pressure, partial oxygen pressure in the arterial postductal blood, and calculating an oxygenation index. If the oxygenation index is 3.5 or less, non-invasive pulmonary ventilation is considered to be effective, whereas the oxygenation index of more than 3.5 shows therapeutic ineffectiveness.

EFFECT: method enables predicting a success rate of various techniques for non-invasive pulmonary ventilation in premature newborns with a very low and extremely low birth body weight diagnosed with respiratory distress syndrome.

2 ex

FIELD: medicine.

SUBSTANCE: patient's pre-operative health is assessed with taking into account the presence/absence of pulmonary and renal diseases, erythrocyte Rh C-antigen, hyperinosemia of more than 4 g/l and erythrocyte count of 4.81×1012/l and less; a haemolytic perfusion risk (HPR) is calculated by formula HPR=(6.38×Xpulm+8.93×Xhyp+3.32×XEr)-(4.10×Xren+2.95×XC), wherein the numerical values 6.38; 8.93; 3.32; 4.10; 2.95 are coefficients; Xpulm is the presence (1)/absence (0) of pulmonary diseases; Xhyp is the presence (1)/absence (0) of pre-operative hyperinosemia; XEr is the presence (1)/absence (0) of the pre-operative erythrocyte count 4.81×1012/l and less; Xren is the presence (1)/absence (0) of renal diseases; XC is the presence (1)/absence (0) of erythrocyte Rh C-antigen; if HPR>6.1, pronounced erythrocyte lysis is predicted; HPR<6.1 shows moderate erythrocyte lysis.

EFFECT: method enables increasing accuracy, information value and accessibility of the erythrocyte lysis prediction.

2 ex, 1 dwg, 3 tbl

FIELD: biotechnologies.

SUBSTANCE: invention can be used for determination of atherogenicity of immune complexes containing multiply modified low density lipoproteins (IC-MMLDLP). For this purpose basic calcium superphosphate of IC-MMLDLP is prepared from human blood serum by treatment with the buffer containing 10% solution of polyethyleneglycol with a molecular weight 3350 (PEG-3350), in the ratio 1:2.5, then it is incubate within 10 minutes at a room temperature. IC-MMLDLP aggregates are pelleted, dissolved in the buffer without PEG-3350, analysed for the content of cholesterol in immune complexes (ChIK) and the level of guinea pig complement binding (EBC) by precipitated immune complexes. IC-MMLDLP atherogenicity is calculated as SSK to HIK ratio. If the value is below 24 units a high blood atherogenicity because of the reduced complement activating IgG function in IC-MMLDLP is stated.

EFFECT: method allows to assess IC-MMLDLP atherogenicity, diagnose atherosclerosis at a preclinical stage, and also to predict both the course of atherosclerotic process at individuals, and efficiency of the conducted therapy.

1 tbl

FIELD: veterinary medicine.

SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.

EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.

2 ex

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