Method for detecting microbial and viral contamination of solutions and biological fluids
SUBSTANCE: invention refers to microbiology, namely to a method for detecting a contamination of solutions and biological fluids. Substance of the method consists in detecting the biological objects containing microorganisms or viruses by means of metal nanoparticles formed in situ from respective metal salts introduced into the analysed object, and analysing the dynamics of spectral characteristics of the forming nanoparticles. In the presence of microbial cells for the period of 20-40 minutes, the size of the forming metal nanoparticles reaches more than 15 nm; in the presence of viruses, the size of the forming nanoparticle reaches 6 nm, whereas the absence of any contamination of the analysed objects is shown by the size of the forming nanoparticle nuclei not exceeding 2 nm.
EFFECT: using the declared method enables the highly sensitive, reliable, technically simple and fast detection of the microbial contamination of solutions and biological fluids.
4 dwg, 3 ex
SUBSTANCE: saliva of affected child is analysed by method of infrared spectroscopy, value of ratio of peak height with maximum at 1070 cm-1 to peak height with maximum 1025 cm-1 is calculated. If value of ratio is in range from 1.1 to 1.9 nonspecific ulcerative colitis is diagnosed, and if value of said ratio is from 2.0 to 4.6 Crohn's disease is diagnosed.
EFFECT: invention ensures less invasive and simpler differential diagnostic of NUC and Crohn's disease in children, ensures simplification and acceleration of diagnosis determination.
SUBSTANCE: invention relates to medicine, namely to a method of diagnosing bipolar affective disorder. The essence of the method consists in the fact that reliable differences in the spectrum of protein distribution in blood serum without proteins albumin, immunoglobulin G, immunoglobulin A, antitrypsin, transferin and haploglobin in patients with endogenic psychosis. If protein spots are detected on electrophoretic gel in areas with the molecular weight of 200, 84, 75, 49, 40 kDa in the patient with endogenic psychosis, bipolar affective disorder is diagnosed.
EFFECT: application of the claimed method makes it possible to increase accuracy in diagnostics being simple in realisation.
SUBSTANCE: method involves determining female serum cytotoxicity to male lymphocytes, including a combined culture with reference male and analysed female serum in a 96-well tray in the presence of the nutrient medium RPMI 1640 in a CO2 incubator. One day later, lymphocytes are counted in the well in a Goryaev's chamber with the male (reference) and female (analysed) serum. That is followed by determining a cytotoxic index (CI), which represents a quotient of the analysed cell count and the reference cell count. The normal cytotoxic index makes approximately 0.7 and less.
EFFECT: invention enables studying the responses of female humoral immune factors to male antigens and evaluating a risk of miscarriage, early spontaneous abortions and missed miscarriages.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a high-sensitivity method for measuring the amount of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts. The high-sensitivity method for measuring the amount of glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts is characterised by the fact that a mixture of individual's blood plasma with methanol or ammonia water in the specific concentration is introduced into a solid phase having the reverse-phase distribution function and the anion exchange function; the solid phase is then washed with a cleaning fluid that is a single-component fluid or a mixed fluid of at least two components specified in a group containing water, alkali, alcohol and acetonitrile. That is followed by elution from the solid phase in acid alcohol specified in formic acid - methanol or formic acid - ethanol; that is followed by the stage of measuring glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts by liquid chromatography - mass spectrometry or liquid chromatography - mass spectrometry/mass spectrometry.
EFFECT: high-sensitivity method enables detecting and measuring the amounts of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts.
4 dwg, 17 tbl, 7 ex
FIELD: veterinary medicine.
SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.
EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.
SUBSTANCE: patient's peripheral venous blood is recovered to analyse genetic polymorphisms of coagulation factors VII 10976G/A FVII. A birth weight of a newborn of a woman delivering not for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6123.431-25.579x1+0.267x2+205.739x3, wherein y is an anticipated newborn's weight, x1 is a female's height in centimetres; x2 is an infant's weight at the previous delivery in grams, x3 is a genetic version of 10976G/A FVII locus with x3=1 for 10976 GG FVII genetic type, x3=2 for 10976 GA and 10976 AA FVII genetic types. A birth weight of a newborn of a woman delivering for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6278.037-21.739x1+232.170x2, wherein x1 is a female's height in centimetres; x2 is a genetic version of 10976G/A FVII locus with x2=1 for 10976 GG FVII locus, x2=2 for 10976 GA and 10976 AA FVII genetic types.
EFFECT: invention enables improving the prevention and treatment of the pregnancy complications.
2 tbl, 4 ex
SUBSTANCE: pre-operative fasting venous blood 1 ml is sampled at room temperature 20-24°C into an anticoagulant-free vacuum system (test tube). The test tubes are delivered in a sealed container at temperature 2-8°C for 2 hours to a laboratory for immunoenzyme assay and analysed to determine anti-thyroid stimulating hormone receptor antibodies. If the antibody level is 1.5 units/l or more, a thyroidectomy is performed, whereas the antibody level of less than 1.5 units/l requires performing a subtotal thyroid resection according to standard techniques.
EFFECT: invention enables reducing a probability of recurrent thyrotoxicosis in the patients after subtotal thyroid resection.
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of a risk of early microvascular complications in the children suffering from type 1 diabetes mellitus. The substance of the method consists in defining a duration of the diseases in years, the patient's age in years, a desquamated endothelial cell count, high-density lipoprotein cholesterol, total cholesterol, triglycerides, atherogenic index, glycohaemoglobin, average daily glycaemic level; making a linear regression analysis and calculating a risk ratio (R) of early microvascular complications in the children suffering from type 1 diabetes mellitus by formula. If the risk ratio is ≥1, the high risk of early microvascular complications during one year is predicted; the ratio < 1 shows the low risk of microvascular complications during one year.
EFFECT: using the declared method enables increasing the prediction accuracy of microvascular complications in the children with type 1 diabetes mellitus.
2 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine and can be used for patient's status monitoring. A method for setting a time-varying physiological parameter warning signal involves patient's controlled parameter monitoring, comparing the controlled parameter to an initial cut-off criterion, varying the cut-off criterion temporarily by a cut-off criterion of deterioration after the therapy, and then after a certain period of time, by the cut-off criterion after the administration. The time allowed involves comparing the controlled parameter to the cut-off criterion of deterioration, and after the time allowed - to the cut-off criterion after the administration. The warning signal is initiated in response to the controlled parameter of one or more initial cut-off criteria, the cut-off criterion of deterioration and the cut-off criterion after the administration. The group of inventions also refers to a machine-readable carrier with software for implementing the method and to a system for user warning on the controlled parameter variation.
EFFECT: group of inventions provides higher accuracy and rate of assessing the patient's status according to the conducted therapy.
15 cl, 6 dwg
SUBSTANCE: invention can be used for the purpose of the early prediction of cystic periventricular leukomalacia (PVL) in the newborns with very low (VLBW) or extremely low body weight (ELBW). Substance of the method: the newborns with VLBW and ELBW on the 3rd-7th day of life are examined to assess the perinatal medical history, namely the presence of chorioamnionitis and amniotic fluid nature, 5th minute Apgar score, the absence of prolonged artificial pulmonary ventilation, a severity of respiratory distress syndrome, the presence of pneumonia, sepsis, convulsive disorder, anaemia, laboratory signs of the systemic inflammatory reaction, average values of carbon dioxide, anionic bicarbonate and base deficiency in capillary blood, interleukine-6 and receptor interleukine-1 antagonist in venous blood serum. Each sign is assigned with a prognostic coefficient (PC). That is followed by determining total PC, and it is expected cystic PVL that is decided for if total PC is at least (+)9.5, whereas no cystic PVL is expected if total PC is (-)9.5 or less.
EFFECT: invention provides the early (for the first 7 days of life) prediction of cystic periventricular leukomalacia in the most susceptible category of patients that are children with very low and extremely low birth weight.
6 tbl, 5 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to chemical-pharmaceutical industry, and can be used in drug quality control centres and analytical laboratories when measuring anthocyanes in such crude drugs, as bilberry, black chokeberry, black currant, etc. The presented method for measuring anthocyanes involves recovering anthocyanes from crude drugs in alcohol water mixtures containing 0.5-1.0% muriatic acid with the ethanol concentration of less than 70% and measuring anthocyanes quantitatively by optical density in 95% ethanol containing 0.5% ammonium as compared to a reference solution at analytical wavelength of peak absorption within the range of 611-630 nm with using a reference solution of cyanidine-3-O-glucoside or with using its specific absorption in 95% ethanol containing 0.5% ammonium.
EFFECT: higher sensitivity and result reproducibility, as well as shortening the analysis procedure as compared to its prototype.
3 dwg, 7 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology, namely, to obtaining inhibitors of adhesion and/or aggregation of platelets, and can be used in medicine. A polypeptide, used as a component of a pharmaceutical composition and in sets for screening of the inhibitors of platelet adhesion or aggregation, is obtained in a recombinant way with the application of a matrix of the salivary gland cDNA of Anopheles stephensi.
EFFECT: invention makes it possible to obtain the polypeptide, possessing inhibiting activity with respect to platelet aggregation and/or inhibiting activity with respect to platelet adhesion.
10 cl, 4 dwg, 5 ex
SUBSTANCE: method is based on measuring a flavonoid amount by differential spectral photometry, calculated as Cinaroside at wave length 400 nm, extracting in water and alcohol and using 70% ethanol as an extraction fluid; the total flavonoid amount calculated as Cinaroside and an absolutely dry raw material in percentage (X) is calculated by formula.
EFFECT: method enables measuring the total flavonoid amount as biologically active components having a basic therapeutic action.
3 ex, 14 dwg, 2 tbl
SUBSTANCE: method of Novocain extraction from water solutions includes the preparation of an aqueous salt solution of Novocain by its dissolution in a saturated solution of a salting-out agent, extraction and analysis of an equilibrium water phase, with the application as an extragent of a solvotrophic reagent solution in chloroform with the concentration of 10 wt %, for which purpose the aqueous salt solution of Novocain with pH 8.0±0.5 is prepared due to the application of a saturated ammonium sulphate solution as the salting-out agent and addition of an ammonium buffer solution, Novocain is extracted for 5-7 minutes with the solution of the solvotrophic reagent in chloroform with the ratio of volumes of the aqueous salt solution of Novocain and extragent of 5:1, then the aqueous saline phase is separated from the organic one and analysed by a method of UV-spectrophotometry at a wavelength of 291 nm, the concentration of Novocain in the water solution is found by means of a calibrating graph; the coefficient of distribution (D) and the degree of extraction (R, %) of Novocain is are calculated by formulae.
EFFECT: method for the extraction of Novocain from the water solution, which is characterised by expressiveness, makes it possible to realise the practically complete single extraction of Novocain from the aqueous salt solution and can be applied in the analysis of Novocain-containing water solutions.
SUBSTANCE: invention relates to analytical chemistry and can be used in a system for monitoring content of sodium thiosulphate in solutions. The method of determining sodium thiosulphate in solutions is characterised by adding an analysed sample into a reaction vessel containing a corresponding amount of photogenerated iodine, obtained by blowing with air for 1-2 minutes and irradiating the reaction mixture with a stabilised light source, the mixture consisting of 0.5 M potassium iodide solution, an acetate buffer solution with pH 5.6 and a sodium eosinate sensitising agent, by detecting change in current in a cell and upon achieving constant current, re-blowing the reaction mixture with air for 2-3 minutes and re-irradiating with the stabilised light source until achieving the initial amount of iodine in the vessel, measuring the iodine generation time spent on achieving the decrease thereof, determining the amount of sodium thiosulphate from a calibration curve from the change in current and generation time.
EFFECT: invention provides a simple method of determining sodium thiosulphate in solutions and avoids use of expensive equipment.
10 tbl, 5 dwg
SUBSTANCE: invention describes a method for lipoic acid measurement in biologically active additives by cathode voltammetry involving transferring a substance from a sample into a solution and taking voltammetric measurement; the cathode voltammetry is performed on a mercury-film electrode at potential -0.373 V of a relatively saturated silver-chloride electrode with borate buffer solution pH 9.18 at continuously current potential trace at 0.06 V/s with the determined lipoic acid content range from 4.5·106 to 1.1·10-3 mole/l.
EFFECT: improving sensitivity and expressivity of the method for measuring lipoic acid in tabletted BAAs by cathode voltammetry.
1 tbl, 1 ex, 3 dwg
SUBSTANCE: in agar plate which is in a Petri dish and inoculated with one of the fungal species of the genus Fusarium, circular holes are cut with the diameter of 8 mm, 0.05 ml of the working solution of the test preparation is applied into them, and placed in a thermostat at a temperature of 24.5-25.0°C, after 2-5 days depending on the type of fungus the diameter of the zone of growth inhibition of fungal mycelium is measured and the activity of the preparations is calculated according to the formula , and it is considered that when A=1 the fungicide is ineffective - the zone of growth inhibition is not formed (D=d), when A=2-3 the preparation activity is low, when A=4 it is average, and when A≥5 it is high.
EFFECT: invention provides accuracy of determining the activity of seed disinfectants and fungicides used in agricultural production.
SUBSTANCE: invention represents a method for preclinical study of cardiotropic antiarrhythmic drugs, involving determining the bioelectric parameters in isolated multicellular perfused preparations and measuring an action potential duration, differing by the fact that the isolated multicellular perfused preparations are presented by rat's pulmonary vein myocardium; the parameters are measured in three operation modes of the multicellular preparations; a resting potential is additionally measured; varying APD 90%, related APD 50%/APD 90%, a spontaneous shear velocity of the resting potential, the most positive membrane potential in the resting preparation, a spontaneous activity train repetition rate, spontaneous action potential train repetition and variability frequency, post-depolarisation number and intensity, as well as a shear membrane potential corresponding to the beginning of train activity are used to evaluate the signs of antiarrhythmic and arrhythmogenic action.
EFFECT: more reliable prediction of the antiarrhythmic action of the potential pharmacological agents and reduction of experimental phase time.
SUBSTANCE: invention relates to method of determining platelet resistance to acetylsalicylic acid (ASA) by impedance analysis of aggregation function of platelets in vivo, in which aggregation activity is analyses after incubation of biological material sample with ASA with application of aggregation inducer, and aggregation of platelets is induced by collagen in optimal concentration 2 mg/ml, and simultaneously with measurement of impedance determination of dynamics of platelet granules release is performed by luminescent method, where before carrying out aggregation samples are calibrated by means of adenosine triphosphate (ATP) standard, value of aggregation amplitude are determined in Ohms by obtained aggregatograms and obtained values are given points: values ≤6 correspond to 0 points, values 7-9 correspond to 1 point, values 10-12 correspond to 2 points, values >12 correspond to 3 points; after that, intensity of ATP release from platelet granules is determined in nmoles and obtained values are given points: values <0.5 correspond to 0 points, values 0.5-1.0 correspond to 1 point, values 1.0-1.5 correspond to 2 points, values 1.5 correspond to 3 points, and then resistance index (RI) is calculated by formula, value of IR parameter points to presence of aspirin-resistance of platelets.
EFFECT: carrying out fast complex diagnostics of platelet resistance to ASA with estimation of functional state of platelet granules before beginning treatment and possibility of preventing development of undesirable thrombotic or hemorrhagic complications.
2 dwg, 1 tbl, 2 ex
SUBSTANCE: claimed is application of fat emulsion for parenteral feeding as solvent for compounds which are poorly soluble in water. Fat emulsion contains in 1 l of solution: 30 g of refined soybean oil, 30 g of triglycerides with the average chain length, 25 g of olive refined oil, 15 g of purified fish oil.
EFFECT: obtaining solvent for compounds, poorly soluble in water, which makes it possible to determine parameters and spectrum of biological activity of novel compounds of chemical nature at the stages of pre-clinical and clinical tests, which does not change basic biological constants and possesses biological inertness.
2 tbl, 2 ex
SUBSTANCE: first step includes obtaining low-hydroxylated insoluble fullerenols by reacting concentrated fullerene solution in o-xylene with aqueous ammonia solution in the presence of a tetrabutylammonium hydroxide phase-transfer catalyst at 35-40°C. At the second step, the obtained low-hydroxylated insoluble fullerenols are hydroxylated to transform them into a water-soluble form by mixing with 6-15% aqueous hydrogen peroxide solution and heating for 4-5 hours at 65°C. Water-soluble fullerenols are then precipitated from an alcohol-containing solution.
EFFECT: simplifying the method while preserving quality characteristics and full extraction of the end product.
2 cl, 1 dwg, 4 tbl, 3 ex