Methods of increasing yield of agricultural crops resistant to 2,4-d

FIELD: agriculture.

SUBSTANCE: to increase the yield of soybean plants resistant to 2.4-D, they are treated with 2.4-D at the growth stage of V3 and R2 with the application rate from 1000 to 2000 g ke/ha. To increase the yield of a soybean plant resistant to 2.4-D: (a) soybean cells are transformed with the molecule of a nucleic acid comprising a nucleotide sequence encoding aryloxyalkanoate dioxygenase (AAD); (b) transformed cells undergo the selection; (c) soybean plants are regenerated from the transformed cells; and (d) 2.4-Dis applied at the growth stage of V3 and R2 of a 2.4-D-resistant soybean plant which is treated with 2.4-D at the application rate from 1000 to 2000 g ke/ha.

EFFECT: invention allows to increase the soybean crop capacity.

18 cl, 3 dwg, 44 tbl, 24 ex

 



 

Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to an isolated nucleic acid molecule which codes a polypeptide, having delta-9-elongase activity, as well as a purified polypeptide having delta-9-elongase activity, which codes said isolated nucleic acid molecule. The invention also discloses an expression vector containing said isolated nucleic acid molecule, as well as a host cell, a transgenic plant and a transgenic seed containing same.

EFFECT: invention provides efficient production of polyunsaturated fatty acid-enriched oils.

18 cl, 9 dwg, 9 tbl, 6 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agricultural biotechnology. Method of producing dihaploid plants of barley from cultured microspores in vitro, comprising: growing donor plants at reduced air temperature of 15-20°C, light regime: 16 h day/8 h night, relative humidity of 60-70%, lighting intensity of 10000-15000 lux, with carrying out phytosanitary treatment, at that growing donor plants is carried out to the stage from opening of the sheath, when the inflorescence is in the flag leaf, - stress treatment of ears at 4°C, in tubes with a poor environment containing KCl - 1.5 g/l, MgSO4×7H2O - 0.25 g/l, CaCl2×2H2O - 0.1 g/l, mannitol - 60 g/l, potassium phosphate buffer - 1 ml/l, pH 7.0, for 7 days for shifting from gametophytic development path of microspore to sporophytic path - microspore isolation from spikelets in sterile conditions, - culturing the isolated microspores in a modified environment for embryogenesis induction, consisting of 6% maltose, 10 sets per 1.5 ml of the culture, and plant growth regulators in amount of 1 mg/l 2,4-D, 0.2 mg/l zeatin, and adding the aforementioned components is carried out before the addition of microspores, - regenerating plants from embryoids by culturing on solid nutrient Murashige and Skoog medium without the adding of plant growth regulators, - processing the barley haploid plants with antimitotic agent N-diacetyl-N-(β,γ-epoxypropyl) aminocolchicine for doubling chromosomes and obtaining dihaploid plants.

EFFECT: invention enables to obtain dihaploid homozygous lines of barley for breeding new varieties and hybrids with improved properties.

1 dwg, 2 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, providing a method for introducing a sequence specific nuclease (SSN) into a plant cell. The method comprising: providing the plant cell having a cell wall; coating a gold nanoparticle with a SSN; placing the cell having a cell wall and the coated nanoparticle in contact with each other; incubating the cell having a cell wall with a coated nanoparticle to allow uptake of the nanoparticle and the SSN into the plant cell comprising a cell wall.

EFFECT: invention provides a method for efficient introduction of SSN into a plant cell comprising a cell wall.

14 cl, 7 dwg, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to use of a protein-coding gene, which consists of an amino acid sequence represented in SEQ ID NO.2, to obtain transgenic rice with long, large and multiple grains and increased yield, more grains per panicle and rolled leaves. Disclosed is use of a transgenic cell line of rice and a recombinant vector to obtain transgenic rice with long, large or multiple grains and increased yield, more grains per panicle and rolled leaves.

EFFECT: invention enables to efficiently obtain transgenic rice with long, large or multiple grains and increased yield, more grains per panicle and rolled leaves.

5 cl, 16 dwg, 4 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention refers to biochemistry, particularly to a method for weed control near sunflower plants. The method involves applying an effective amount of any of the herbicides specified in a group consisting of imidazolinone herbicide, sulphonylurea herbicide, triazolopyrimidine herbicide, pyrimidinyloxybenzoate herbicide and their mixture, on weeds and sunflower plants. The above sunflower plant contains in its genome at least one copy of at least acetohydroxyacid synthase (AHAS) gene coding a herbicide-resistance protein having an amino acid sequence presented by SEQ ID NO:2.

EFFECT: invention enables controlling weeds near sunflower plants effectively.

9 cl, 13 dwg, 14 tbl

Farnesene-synthase // 2546877

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biotechnology. Claimed are: a separated from the tomato polynucleotide, corresponding to SEQ ID NO:1, presented in the description, or a polynucleotide, which has, at least, a 70% identity with the nucleotide sequence SEQ ID NO:1, or their fragment, which code a polypeptide with the activity of farnesene-synthase, corresponding to SEQ ID NO:2, presented in the description, or having, at least, a 70% identity with an amino acid sequence, corresponding to SEQ ID NO:2, or their fragment. Genetic constructions, containing the said polynucleotide, where genetic constructions represent a transformation vector and an expression vector are described. Described is the transformation vector, which contains the polynucleotide which is hybridised with a polynucleotide, coding the said polypeptide. A host cell for expression farnesene-synthase, which contains a genetic construction, selected from the said ones, is described. Claimed is a method of obtaining alpha-farnesene and/or beta-farnesene, including stages of the said host cell cultivation with obtaining the increased activity of alpha-farnesene synthase and/or activity of beta-farnesene-synthase and optionally obtaining a cell with farnesyldiphosphate; and the separation of the obtained alpha-farnesene and/or beta-farnesene. Described is a method of modulating the production of alpha-farnesene and beta-farnesene by a plant, which includes an increase or reduction of expression of the said farnesene-synthase, where the said increase or reduction is achieved by means of genetic modification to change the expression of polynucleotide, coding the said polypeptide. Claimed is the application of the polynucleotide in accordance with the claimed invention for coding a molecular (DNA) marker, bound with the said nucleotide sequence.

EFFECT: invention makes it possible to obtain alpha-farnesene and beta-farnesene in the host cell.

16 cl, 8 dwg, 1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to biochemistry, particularly to a gene expression construct containing: (a) an expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, b) a polylinker for facilitating a gene insertion into a gene expression system. What is disclosed is a gene expression vector containing the above construct and heterologous gene. What is also described is the gene expression vector containing: (a) the expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, (b) the heterologous gene, which codes a protein of interest, as well as a method for expressing the protein of interest with using it. A method for increasing translation enhancement activity of the heterologous protein of interest from the heterologous gene coding the above protein, a method for translation enhancement of the heterologous protein of interest from the heterologous gene or a heterologous open reading frame coding the above protein are disclosed. There are also described a host organism transformed by the above vector, as well as a method for producing the protein of interest, involving collection of the host organism tissue.

EFFECT: invention enables enhancing translation of the heterologous protein of interest from the heterologous gene.

26 cl, 15 dwg, 4 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biochemistry, in particular to a method of increasing biomass or improvement of cell proliferation, leaf development and/or reproductive development of a plant, which includes increasing expression in the plant of nucleic acid, coding the ubiquitin-specific protease, as well as to the plant, a part of the plant and a seed of the plant, biomass of which is increased, or cell proliferation, leaf development and/or reproductive development of which are increased by the said method. A vector for application in the method of obtaining plants with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the plant and the part of the plant, transformed with the said vector, are described. Application of nucleic acid, coding the ubiquitin-specific protease is disclosed. Also disclosed is a method of obtaining a transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development in comparison with the control plants, obtained as a result of increased expression of nucleic acid, coding the ubiquitin-specific protease.

EFFECT: invention makes it possible to efficiently obtain the plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development.

17 cl, 11 dwg, 3 tbl, 28 ex

Transgenic plants // 2515927

FIELD: chemistry.

SUBSTANCE: invention relates to field of biochemistry, in particular to genetic construction for expression of threonine-insensitive aspartate kinase in plant, where construction contains promoter, specific for phase of aging, functionally connected with coding sequence, which codes polypeptide, possessing activity threonine-insensitive aspartate kinase, and in which specific to aging promoter is selected from group, consisting of SAG12, SAG13, SAG101, SAG21 and SAG18, or their functional variants or fragments. Described are vector, plant cell, transgenic plant, material for plant reproduction, collected leaves, smoking tobacco, which contain claimed genetic construction. Also described is method of obtaining transgenic plant, which possesses ability to accumulate threonine in fading leaves in larger amount than in leaves of corresponding plant of wild type, and method of increasing the level of threonine in leaves of aging plant to the level of threonine, higher than the content of threonine in leaves of plant of wild type without impairing plant adaptation with application of said genetic construction.

EFFECT: invention makes it possible to obtain transgenic plant, which possesses ability to accumulate threonine in fading leaves in larger amount than in leaves of plant of wild type.

22 cl, 16 dwg, 2 tbl, 4 ex

FIELD: chemistry.

SUBSTANCE: invention relates to field of biochemistry, in particular to methods of targeted change of duplex acceptor DNA-sequence and improvement of efficiency of targeted mutagenesis in plant protoplasts. Claimed methods include introduction into protoplasts of plant cells of single-stranded mutagenic oligonucleotide, which in its composition has, at least, one wrong nucleotide with respect to duplex acceptor DNA-sequence, which is to be changed with application of PEG-mediated transformation.

EFFECT: invention makes it possible to increase frequency of targeted mutagenesis.

10 cl, 3 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture. Fertiliser-pesticide mixture for cereal crops contains liquid nitrogen fertilisers (UAN-32), water, cowboy super and kinmix with the following component ratio, wt %: (UAN-32) - 30.0-40.0; cowboy super - 0.11-0.20; kinmix - 0.2-0.5, water is the rest up to 100.

EFFECT: invention enables to reduce the rate of application of herbicide, to reduce the pesticide load on the environment.

2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention refers to an aqueous catheter lock solution which contains citrate, photochemical oxidant methylene blue and dispersed or dissolved paraben. The invention refers also to a catheter locking kit and an infusion device containing the aqueous catheter lock solution, and also to a method of prevention or delay of blocking or contamination of a permanent catheter.

EFFECT: catheter lock solution of density appropriate to be held in the catheter while locked, allows eliminating infection and cutting its probability while carrying a catheter.

28 cl, 6 tbl, 1 dwg, 9 ex

FIELD: agriculture.

SUBSTANCE: agent for treatment of sugar beet to protect from storage decay containing aqueous solution of fungicide is described. Derivatives of carboxylic acid are used as fungicide and has formula RCOOX, where X=H, Na, K, C1-C10-alkyl, NH4, R=H, CH3, C2H5, C6H5, CH3-CH=CHCH=CH-, excluding propanoic acid and its salt with consumption rate of 0.1-100 hectare/t.

EFFECT: increase of efficiency while controlling storage decay and decrease of sugar lost during storage.

3 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to means for plant protection. Composition in form of microemulsion consists of organic phase, which includes tebuconazole as active component, as well as emulsifier and solvent, and water phase, which includes water, diatomic alcphole and dye. In organic phase it additionally contains imazalyl as active component with ratio tebuconazole : imazalyl 0.35÷1.55:1. Composition can additionally contain in organic phase indolylacetic acid or ambiol as regulator in amount 0.005-1.0% from sum of active substances and imazalyl and tebuconazole.

EFFECT: invention allows to increase composition stability.

1 tbl, 1 ex, 2 cl

FIELD: agriculture.

SUBSTANCE: a herbicidal compound containing as the active substance a mixture of salts of the 2-methoxy-3,6-dichlorobenzoic acid (dicamba) (I) and herbicides from the row of substituted sulfonylureas selected from among chlorsulfuron (IIa) or tribenuron methyl (IIb) and metsulfuron methyl (III) at a ratio of I:(IIa or IIb): III, equal to (15- 20):(1-3):(0.5-2.0) according to the acid equivalent, is described. The compound also contains surfactants and filling materials. A synergic effect is observed. The formulations are wettable powders and water-dispersible granules.

EFFECT: compound is effective against wide spectrum of weeds in agricultural crops.

5 cl, 8 tbl, 6 ex

FIELD: agriculture.

SUBSTANCE: claimed method includes seeds spraying with mixture of titus herbicide (30 g/hectare) and banvel herbicide (300 ml/hectare) with addition of surfactant-trend (300 ml/hectare) with consumption of processing liquid of 300 l/hectare.

EFFECT: method for weed controlling of increased effectiveness.

1 tbl

FIELD: biotechnology, agent for combating of domestic dust mites and mite allergens in process of fabric article washing.

SUBSTANCE: claimed method includes blending of benzyl benzoate with surfactants such as Tween-80 and Tween-30 and water and introducing of obtained acaricidal mixture into detergent. 33-34 % Ammonium sulfate aqueous solution is introduced into mixture of benzyl benzoate, Tween-80 and Tween-30 in ratio of 3:2:2 in amount of 76.6 ml per 100 ml of mixture. Further mixture is stirred to produce homogenous acaricidal emulsion followed introducing thereof into detergent in amount of 1-2 ml per 1 l of detergent at 30-60°C. Obtained preparation has storage time of 1 year in dark bottles.

EFFECT: agent with decreased toxicity, prolonged storage time and increased acaricidal activity.

3 ex

FIELD: horticulture, in particular, propagation of planting stock and growth promoters.

SUBSTANCE: method involves treating cuttings with growth promoting substances upon exposure to aqueous solution of stimulant, namely, para-aminobenzoic acid (PAB) used in concentration of 0.02-0.05% during 5-10 hours; thereafter, coating cuttings with 2-3 cm thick zeolite-containing clay layers and planting into soil.

EFFECT: increased amount and improved retention of planting stock, reduced susceptibility to various diseases and intensified growth of cells.

2 tbl, 2 ex

FIELD: biotechnology, in particular agent for removing of mite allergens and domestic dust mites.

SUBSTANCE: claimed method includes blending of benzyl benzoate with surfactants and water and introducing of obtained acaricidal mixture into detergent. As surfactants Tween-80 and Tween-20 are used. Copper sulfate and distilled water are introduced into mixture of benzyl benzoate, Tween-80 and Tween-20. All components are used in specific ratio. Abovementioned components are intensively agitated to produce homogenous acaricidal emulsion. Obtained emulsion are introduced into detergent in amount of 1-2 ml per 1 l of detergent at 30-60°C.

EFFECT: agent of decreased toxicity and increased acaricidal activity.

1 tbl, 3 ex

FIELD: agricultural engineering.

SUBSTANCE: composition has dialene, mixture of potassium and sodium dimethylammonium salts of MCPA (agritox) and surfactant agent of trend in mass proportion of ingredients equal to 2.06:1, respectively. Method for eliminating weeds or undesired plants involves applying tank mixture of dialene at a dose of 1.75 kg/hectare and agritox at a dose of 0.85 kg/hectare. Surfactant agent of trend is added to the mixture in the amount of 200 ml/hectare with normal working liquid expenditure being equal to 200 l/hectare in cereals and lawn grass crops.

EFFECT: wide range of herbicide action spectrum.

3 cl, 2 tbl

FIELD: agricultural engineering.

SUBSTANCE: composition has dialene, mixture of potassium and sodium dimethylammonium salts of MCPA (agritox) and surfactant agent of trend in mass proportion of ingredients equal to 2.06:1, respectively. Method for eliminating weeds or undesired plants involves applying tank mixture of dialene at a dose of 1.75 kg/hectare and agritox at a dose of 0.85 kg/hectare. Surfactant agent of trend is added to the mixture in the amount of 200 ml/hectare with normal working liquid expenditure being equal to 200 l/hectare in cereals and lawn grass crops.

EFFECT: wide range of herbicide action spectrum.

3 cl, 2 tbl

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