Microbial inoculants and fertilisers composition containing them
SUBSTANCE: microbial inoculant for increasing of plant growth, plant productivity and/or soil quality contains strains of two or more species of bacteria selected from Lactobacillus parafarraginis, Lactobacillus buchneri, Lactobacillus rapi and Lactobacillus zeae. The microbial inoculant contains at least one bacterial strain selected from Lactobacillus parafarraginis Lp18, Lactobacillus buchneri Lb23, Lactobacillus rapi Lr24 and Lactobacillus zeae Lz26. Application of inoculant as a fertiliser. The fertiliser composition contains a microbial inoculant. Application of the fertiliser composition. A method for increasing plant growth and/or productivity. A method to improve the quality of soil. Method for degraded soil or pasture recultivation.
EFFECT: inventions make it possible to obtain fertilisers based on microorganisms that are effective in provision of nutrients for plant growth, and are environmentally safe.
35 cl, 11 dwg, 5 tbl, 9 ex
SUBSTANCE: method comprises applying to fermented milk products by mixing of liquid culture of strain of propionic acid bacteria Propionibacterium freudenreichii R13-slg RNCIM B-11325 in the amount of 0.5-2.5 vol. % or freeze-dehydrated powder of this culture in an amount of 0.04-0.10 wt %, after which the product storage at 0-4°C for up to 2-3 months, at 20-22°C for up to 30-35 days and at 30-35°C for up to 2-8 days.
EFFECT: enrichment of fermented milk products with viable probiotic cells and vitamin B12 and safety of fermented milk products, which is recorded by the absence of fungal growth, the absence of change of taste, smell, formation of bubbles.
2 dwg, 1 tbl, 23 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to health-promoting compositions and methods for preparing them. A method for preparing the composition of non-living lactic acid bacilli, possessing an ability of specific binding to Streptococcus mutans, involves the following stages: heating a cell suspension of lactic acid bacillus or a mixture of lactic acid bacilli possessing an ability of specific binding to Streptococcus mutans from an initial temperature of less than 40°C to a pasteurisation temperature of 75 to 85°C with a temperature variation within the range of 0.5 to 2°C/min, keeping the heated suspension at a pasteurisation temperature of 20 to 40 minutes and cooling the suspension to a final temperature of less than 40°C within the range of 0.5 to 2°C/min. The specific binding the cell suspension of the lactic acid bacillus or the mixture of lactic acid bacilli to Streptococcus mutans is stable to heat treatment and/or resistant to proteases and/or calcium-dependent and/or is observed within the range of pH values falling within the range of 4.5 and 8.5, and/or in the saliva environment.
EFFECT: invention enables producing the agent preventing or delaying the caries lesion formation.
9 cl, 4 ex
SUBSTANCE: method of obtaining of brucellous L-antigen is performed as follows. The strain Brucella abortus 19 is cultivated in the L-form in the dense nutrient medium prepared on the basis of meat-peptonic hepatic glucose-glyceric agar (1.3-1.5%) with adding of 10-15% normal horse Serum at the temperature 20-22°C within 2-3 days. The culture, obtained in the L-form, is emulsified with 0.5% phenolised normal saline solution, inactivated at the temperature 85-90°C within 60 minutes. The suspension is centrifuged at 3000-5000 rpm within 15-20 minutes and the concentration of brucellas is established at the level 50-60 billion m.k. The obtained antigen is titrated, standardised by specificity and activity. It is used for agglutination test in serum diagnostics of brucellosis.
EFFECT: invention allows to minimise the time of antigen preparation and to increase the yield of bacterial mass.
4 tbl, 3 ex
FIELD: machine building.
SUBSTANCE: vortex bioreactor includes cylindrical vessel1 with lid 2 provided with a device for medium mixing. Mixing device consists of bladed wheel 7, horizontally fixed on vertical shaft in upper part of vessel 1 and horizontal annular partition 9, installed in vessel 1 with a gap relative to its cylindrical walls, rod 10 vertically installed along axis. Horizontal annular partition 9 is located with possibility of rotation on vertical rod 10 and has retention mechanism of horizontal annular partition 9, installed on rod 10. Bioreactor is provided with pipe 19 or telescopic pipe 20, mated to axial hole 13 of horizontal annular partition 9, attached to the bottom of the last one and located around rod 10. On upper surface 11 of horizontal annular partition 9 there made are radial channels 12, located from axial hole 13 to the edge of annular partition 9 inclined downwards to the bottom of vessel 1. Horizontal annular partition 9 is designed floating, for example, from polypropylene. Inner diameter of pipe 19 or telescopic pipe 20 corresponds to diameter of axial hole 13 of horizontal annular partition 9. Area of axial hole 13 of horizontal annular partition 9 equals to cross sectional area at inlet 14 to radial channels 12 and total cross sectional area at outlet 15 from these radial channels 12.
EFFECT: improving efficiency of mixing and speeding up biochemical processes upon their carrying out using fluid mediums of various viscosity.
6 cl, 5 dwg
SUBSTANCE: banana fruit and skin are weighed, washed, crushed and mixed with water preheated to 45±1°C in the ratio of 1:3. It is alkalised to pH 8.2-8.3, the enzyme is added, which is used as the pancreas of cattle in the amount of 9-15% of total volume. The preservative is added, which is used as chloroform in an amount of 1% of total volume. The mixture is subjected to hydrolysis for 10 days at a temperature of 45-50°C. During the first 24 hours the mixture is stirred every 15 minutes for 5 minutes, and then every 2 hours for 5 minutes. The hydrolyzate is filtered through cheesecloth, then the filter towels (paper) and sterilised by ozonation. It is covered with cork and stored hermetically sealed at a temperature of from 2°C to 8°C.
EFFECT: invention provides obtaining of transparent hydrolyzate with increased sterility, with high biological activity, which leads to improvement of its properties when it is used as a growth stimulator of Leptospira.
2 tbl, 3 ex
SUBSTANCE: method provides culturing in the liquid culture medium of the virulent strain of bacteria Staphylococcus aureus No. 6 followed by separation of the culture medium from the bacteria by filtration through the filter PLASMAFILTER PLASMAFLUX PSu 2S with obtaining the filtrate. Ammonium sulphate to 80% saturation is added to the resulting filtrate to obtain the precipitate. The resulting precipitate is separated by centrifugation at 10000 g for 30 minutes and dissolved in phosphate buffer at pH 7.4 with subsequent microfiltration of the protein-containing fraction through the 0.22 mcm membrane and desalting on the column PD-10, purification and concentration by carrying out ion-exchange chromatography on the column of Q-sepharose, elution with 0.15 M NaCl and ultrafiltration on two filters of 100 and 30 kDa to obtain protein containing fraction with the molecular weight of 30-90 kDa and protein content of 0.5-1.0 mg/ml.
EFFECT: invention enables to obtain the products based on protective secreted protein-containing compound.
1 dwg, 5 tbl, 1 ex
SUBSTANCE: method to cultivate sublimated strains of microorganisms includes introduction of a growth stimulant and a source of carbon into a dense nutrient medium with subsequent seeding of microorganism cells, incubation of seeds and accounting of viable microbial cells. Sublimated strains of microorganisms include Yersinia pestis EV, Escherichia coli C600, Bacillus anthracis "СТИ", Vibrio cholerae NAG 504, Brucella abortus 19BA. The growth stimulant and carbon source is 96% ethyl alcohol in the amount of 1.0-2.0% of the medium volume. If necessary, they add gentian violet in concentration of 1:100000.
EFFECT: improved yield of viable strain cells on dense nutrient media.
1 tbl, 5 ex
SUBSTANCE: two suspensions are prepared. Clinical polyantibiotic-resistant strains Escherichia coli are added to an isotonic solution of NaCl to achieve the concentration of 30-40 thousand CFU/ml. Copper nanoparticles are added to the solution of NaCl to achieve the concentration of 0.01-0.05 mg/ml. The suspension of ethylenediaminetetraacetic acid - EDTA is prepared by its dilution in distilled water at the rate of 0.1-0.2:1, respectively. NaOH is added to the prepared suspension to obtain the solution of EDTA with pH=7.6-8. The prepared suspensions are connected with the solution of EDTA in the following ratios by wt %: suspension of copper nanoparticles - 70-85, suspension of microorganisms - 10-20, EDTA - 5.10. It is incubated in the shaker at 100-150 rev/min and a temperature of 36-38°C for 40-60 minutes. The resulting biomass is inoculated on the solid nutrient medium with the volume of 20-25 ml in the amount of 0.1-0.12 ml. It is incubated in the thermostat at a temperature of 36-38°C for 18-24 hours. The sensitivity of E.coli strains to antibiotics is determined.
EFFECT: invention enables to increase the sensitivity of the said bacterial strains to antibiotics gentamicin and ampicillin.
2 tbl, 2 cl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, namely to leukolectins, and can be used in medicine. What is prepared is the polypeptide leukolectin characterised by SEQ ID NO:1-8. The recombinant preparation is ensured by using a nucleic acid coding it and integrated into an expression vector which is used to transform a host cell. Testing absence-presence or determining an amount of the polypeptide leukolectin are ensured by using an antibody or an antigen-binding fragment of a variable region of the above antibody which is specifically bound to the polypeptide leukolectin. The polypeptide leukolectin or the nucleic acid coding it are used as ingredients of a pharmaceutical composition in therapy of pathological disorders of skin and mucous membranes.
EFFECT: invention enables treating or preventing autoimmune disorders of skin, inflammatory diseases of skin or mucous membrane, or injured skin in an animal effectively.
16 cl, 19 dwg, 3 tbl, 12 ex
SUBSTANCE: soil contaminated with oil products is detoxified by adding a natural sorbent with a biopreparation until a given concentration of the contaminant in the soil is achieved. The sorbent used is glauconite and the biopreparation used is polyculture. Before adding the sorbent with the biopreparation, concentration of the contaminant is measured, the volume of sorbent is determined and a shielding intermediate layer of biohumus is placed at the boundary where the contaminant penetrates the soil in the lower part of the contaminated layer. Concentration of the contaminant is determined layer-by-layer. The number of layers of contaminated soil with concentration of the contaminant in the layer differing from each other by at least double is not less than two. The contaminated soil is moistened after distributing the volume of sorbent with the biopreparation, effective microorganisms and fermented compost on the surface of the contaminated soil while simultaneously mixing. A water-swelling polymer is also added to the contaminated soil. Temperature conditions for activating biological processes are maintained by adding organic materials to the soil, wherein the volume of said organic materials in the shielding intermediate layer should be at least double that in the contaminated soil.
EFFECT: high efficiency and faster detoxification.
SUBSTANCE: invention relates to the field of processing wastes into compost. The processing line comprises devices of receiving, humidification, unloading, and modules made of heat-insulating enclosure panels. The modules are mounted sequentially in cascade coming down from the first to the final. The output part of the previous module is mounted above the inlet part of the next module. At the end of each module a stirring device is mounted in the form of horizontal shafts with beaters, and a hopper with dispenser of additives. The modules are equipped with conveyors and individual ventilation system with the ability of automatic regulation of the exchange and temperature of the air. The terminal devices of the ventilating system are made in the form of perforated air ducts. The air ducts are arranged along the conveyors and in the side walls of the modules with the descend to the final relative to each other. The modules comprise the moulding and the working chambers. The chambers are integrated by conveyors and blocked off by the valves-flanges. At least two modules are formed as modules of composting and germination of mycelium. The line comprises at least two modules of composting. At the beginning of the modules of composting the alignment devices are mounted.
EFFECT: reduction of time of composting and increase in the efficiency of waste processing is ensured.
2 cl, 2 dwg
SUBSTANCE: inventions relate to agriculture. Biopreparation for increasing crop productivity includes mixture of live microbial cells in form of culture liquid, containing as microorganisms Lactobacillus casei (ARSIM, B-11360) in amount 4-7% of preparation volume with number of cells 2-4 bln/ml, Lactococcus lactis (ARSIM, B-11341) in amount 8-11% of preparation volume with number of cells 0.5-1.0 bln/ml, Rhodopseudomonas palustris (ARSIM, B-11340) in amount 1/5 of preparation volume with number of cells 1-2 bln/ml, Sacharomyces cerevisiae (ARSIM, Y-3964) in amount 3-7% of preparation volume with number of cells 100-300 mln/ml and additionally contains sodium ascorbate and microelements B, Mn, Fe, Cu, Zn in the total amount respectively 0.7-1.0% and 0.1-0.5 mg/l of preparation volume. method of obtaining biopreparation for increasing crop productivity includes mixture of live microbial cells in form of culture liquid, with introduction into prepared sterile nutritional medium of inoculation material Lactobacillus casei (ARSIM, B-11360), Lactococcus lactis (ARSIM, B-11341), Sacharomyces cerevisiae (ARSIM, Y-3964), introduction of half of counted amount of microelements B, Mn, Fe, Cu, Zn and 2/9 of counted amount of sodium ascorbate, with further carrying out anaerobic fermentation, bringing pH value to 4.5 and introduction of counted amount of Rhodopseudomonas palustris (ARSIM, B-11340); after mixing, after fermentation stops, the remaining amount of sodium ascorbate and microelements is introduced.
EFFECT: inventions make it possible to increase quality of agricultural products, activate growth and form final production of processed plants.
2 cl, 2 tbl, 3 ex
SUBSTANCE: bacterial fertiliser contains a bacterial association including stocks of microorganisms Bacillus mycoides var. B.A. VNIISKhM No. D138 and Azotobacter vinelandii var. NP VNIISKhM No. D24 and composite compound of components, at that association of stocks of the microorganisms Bacillus mycoides var. B.A. VNIISKhM No. D138 and Azotobacter vinelandii var. NP VNIISKhM No. D24 are taken in ratio (1.5-2.5): 0.8-1.4), and in composite compound potassium lingo-humate, microelements in chelate form based on EDTA and/or OEDF, amber and oxalic acids are used. All components are taken at definite ratio.
EFFECT: invention extended scope of application of the fertiliser for wide class of soils and different plant cultures.
9 cl, 3 ex
SUBSTANCE: method comprises the basic tillage, sowing, care and harvesting. And the soil tillage is carried out with chisel tool with the formation of ridge bottom of the furrow, and sowing the crop is carried out over the deepening of the furrow bottom in every second deepening in the first year of sowing. In the second year the sowing is carried out over the unused deepening of the furrow bottom of the first year, and the width of row-spacing after tillage is equal to half the distance between the rows. The direction of sowing the crop is oriented perpendicular to the motion of the dominant wind. Sowing the crop over the deepening of the furrow bottom alternates with the steam bands, which are loosened during the growing season to the depth of 0.08-0.12 m at least 2 times. After harvesting the crops the plant residues are treated with biomineral preparation of nitrogenous fertilizers, complex humic concentrate and water taken in the ratio of 5:0.2:94.8 based on 310-320 kg per hectare.
EFFECT: method enables to preserve the soil fertility, destroy weeds, obtain quality products and save seeding material.
5 cl, 4 dwg, 1 tbl
SUBSTANCE: invention relates to agriculture and can be used in the production of a peat-humic preparation used for root and foliar application of different crops in the open and closed ground, as well as a biologically active supplement for animals and fish. The dry peat-humic compound comprises a mixed composition on the basis of peat of preliminarily shredded peat and a biologically active organic supplement. The method of production of the dry peat-humic preparation comprises crushing, mixing and drying the organic raw material consisting of peat and the biologically active organic supplement to obtain the dry peat-humic product in the form of a homogeneous equally-dispersed mixture. The biologically active organic supplement is used as a plant composition consisting of needles of Scotch pine, Rhodiola rosea root, grass and root of anomalous peony, grass of rape and of Echinacea purpurea in the flowering stage to obtain an intermediate dry peat-humic product which is additionally crushed for 13-15 minutes, divided into four equal initial fractions, into each of which added is with stirring one dry culture of bacteria Bacillus subtilis, Bacillus licheniformis, Arthrospira fusiformis and Actinomyces. Mixing is carried out separately with each dry bacterial additive in a mixer with a water jacket, followed by chilling. Then, mixing of all received components is carried out to a homogenous mass in the mixer with a water jacket, followed by chilling the resulting mixture. Stirring is resumed to chilling to 9-11°C. The resulting ready dry peat-humic preparation containing at least 106 CFU/g dry culture of each bacterium is unloaded onto an open platform. After exposure for 24-26 hours it is packed in bags and exposed for 40-48 hours more.
EFFECT: embodiment of the inventions provides obtaining the dry peat-humic preparation having higher efficiency due to the high level of the bacterial content in the preparation, enhancing biochemical and microbiological processes in soil and plants that improve plant nutrition, effectively suppresses pathogenic and potentially pathogenic microorganisms in soil and plants.
SUBSTANCE: group of inventions relates to agriculture. The air biosoil - an artificial soil consists of large clumps of 2-10 mm in size, uniformly distributed over the volume, the small clumps of 0.1-0.3 mm in size, and the ultrafine clumps of up to 10 microns, with a total porousness of at least 40%, with a content of organic matter of not less than 20%, the acidity of pH 6-8, having a colour from light brown to black, containing microflora characteristic to wild black soil. The method of air biosoil preparation consists in the fact that waste products of animals and other organo-mineral wastes are mixed, peat, scanty soil and other filling materials are added, the resulting mixture is crushed to obtain at least 40% of small clumps, 1-5% of mixture is selected and passed through a disintegrator to obtain ultrafine clumps, they are mixed with the rest of the mixture, the concentrated soil solution is added in an amount of 1-3% by volume of the mixture, then the sorbent additives are added to provide porousness of at least 40%, the mixture is stirred by pouring to the containers with the displaced centre of gravity to ensure homogeneity , the mixture is placed into a fixed container and maintained at an optimum temperature within 20-37°C for at least 24 hours.
EFFECT: invention enables to increase the humus content and to improve productivity of creating soil from wastes.
SUBSTANCE: method of production of biofertiliser comprises obtaining of bio-mixture by adding microbial cultures Pseudomonas sp. 114 deposited in the RNCIM under the number of B-5060, and Azotobacter chroococcum B-35deposited in the RNCIM under the number of B-6010, with a titre of 108 cells/ml at a ratio of 2:1 on a dry combined carrier with the rate of 60 ml per 1 kg and stirring. The carrier is used as cellulose-containing substance such as sunflower or rice husks and mineral-containing component such as perlite, taken in the ratio of 1:3 by weight, then the bio-mixture is applied to the floor of poultry premises at a dose of 30-70 g per 1 m2 at a humidity of the carrier of 15-20%, then the bio-mixture with the wastes of poultry premises is collected while accumulated and stored in piles.
EFFECT: invention enables to accelerate the process of bioconversion with simultaneous increasing the biological activity of the conversion product and environmental safety.
5 tbl, 1 ex
SUBSTANCE: method of increasing soil fertility includes pre-sowing treatment of Lucerne seeds with liquid biopreparation, cultivation and mowing of lucerne green mass. For seed treatment used is liquid bacterial biopreparation based on strain Sinorhizobium meliloti Yakutskiy No 2 SRI ARRIAM RCAM00826. Said preparation is taken in amount 1 billion of cells per 1 lucerne seed.
EFFECT: invention will make it possible to increase humus content in soil under old-aged lucerne grass stand.
2 ex, 2 tbl
SUBSTANCE: invention relates to biotechnology and can be used in microbiology. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, phosphorite and distilled water in a given ratio.
EFFECT: invention speeds up growth of nitrogen-fixing and phosphate-mobilising microorganisms.
1 tbl, 12 ex
SUBSTANCE: invention relates to biotechnology and can be used in microbiology. The culture medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, vermiculite and water in a given ratio.
EFFECT: invention speeds up growth of nitrogen-fixing and phosphate-mobilising microorganisms.
1 tbl, 12 ex
SUBSTANCE: invention relates to the field of agriculture. The method comprises searching on the arable land of sources of crop disease development, pest propagation, depressed areas requiring treatment. The characteristics of video images of crops in different areas of the arable land are compared with the database, and the presence of deviation of these characteristics from the standard. The sources of crop disease development, pest propagation, depressed areas requiring treatment are determined. Anthropogenic impact on the technological processes of crop cultivation on each of the revealed source of crop disease development, pest propagation, depressed areas of the arable land, which requires treatment, is carried out right after their determining. Impacts on the mentioned areas are performed by robotic devices, during their functioning the harmful effects on soil and crops are eliminated. In depressed areas requiring treatment or stimulation of growth and development of crops, impacts on crops and soil are carried out using laser radiation. The impacts on the sources of pest propagation, crop disease development are treated with gaseous substances. The device comprises an unmanned flying platform, which comprises a visual unit, a navigation unit, an information transfer unit, a control unit. The device also comprises a control system for comparing the video images connected with the information transfer unit, the unit of processing the crops with laser, the unit of processing the crops with gaseous substances. The outputs of the visual unit, the navigation unit, the information transfer unit are connected respectively to the first and second inputs of the control unit. The inputs of the unit of processing the crops with laser and the unit of processing the crops with gaseous substances are connected to the first and the second outputs of the control unit, respectively.
EFFECT: such technology and constructive implementation enable to improve the efficiency, responsiveness, to reduce the time of processes of elimination of sources of crop disease development, pest propagation, treatment of depressed areas of arable lands.
2 cl, 2 dwg