Microencapsulated bacterial consortium for gluten degradation in fermented dough and method for obtaining said fermented dough

FIELD: food industry.

SUBSTANCE: microencapsulated bacterial consortium for gluten degradation contains Lactobacillus plantarum ATCC 8014, Lactobacillus sanfranciscensis ATCC 27652 and Lactobacillus brevis ATCC 14869, encapsulating agents, prebiotics selected from the group including polydextrose, inulin and agave syrup, and trehalose in combination with a proteolytic enzyme of bacterial origin and a proteolytic enzyme of fungal origin commonly used for baking. The method for obtaining a consortium for gluten degradation comprises the stages of separately reacting each strain of lactic acid bacteria, separately cultivating each activated strain in a liquid culture medium until each strain reaches a predetermined concentration, removing for each strain the culture medium excess for concentrating the microorganisms, preferably by centrifugation, with obtaining granules, separately resuspending the granules for each strain in a suspension of the saline solution and adjusting to a predetermined volume, mixing the required amounts of each strain and adjusting to a final volume, dissolving the encapsulating agents in water at suitable proportions of dissolved dry substances from 20 to 30%, adding prebiotic and trehalose, inoculating the mixture of the three strains of lactic acid bacteria with obtaining approximately 1010 CFU of said mixture of three strains of lactic acid bacteria per gram of the encapsulated powdery substance, and spray drying with the inlet temperature of 110 to 160C and the outlet temperature of 60 to 80C, at a feed rate of 20 to 50 ml/min. Invention also relates to fermented dough, the method for its obtaining, and sweet baked products obtained from the fermented gluten-free dough.

EFFECT: group of inventions allows to obtain a consortium which is stable and quickly activated.

17 cl, 4 tbl, 6 ex

 



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: biotechnology.

SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.

EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.

4 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: nutrient medium comprises sodium chloride, potassium chloride, magnesium sulphate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, sodium bicarbonate, L-arginine, L-glutamine, L-tyrosine, L-tryptophan, calcium pantothenate, pyridoxal HCl, thiamine, meso-inositol, nicotinamide, riboflavin, folic acid, glucose, enzymatic hydrolyzate of muscle proteins, lactalbumin enzymatic hydrolyzate, sodium salt of benzylpenicillin with activity of 0.09105-1.1105, kanamycin sulphate with activity of 0.09105-1.1105, nystatin with activity of 6104 to 6.5104 IU/l, choline chloride, succinic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to improve the quality of the product obtained.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.

EFFECT: invention makes it possible to increase the output of citric acid.

2 tbl, 6 ex

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns the strain Escherichia coli BL21(DE3)Gold/pETmin-CypA producing human recombinant cyclophilin A. The characterised strain is produced by cell transformation of the strain BL21(DE3)Gold by pETmin-CypA plasmid. The plasmid has a size of 5865 base pairs and contains XhoI-NdeI fragment of pET-22b(+) vector carrying ampR ampicillin resistance gene, a promoter and RNA-polymerase T7 phage terminator and a polylinker. A fragment produced by PCR with using the oligonucleotide primers 5'-TTATACATATGGTCAACCCGACCGTGTTCTTC and 5'-TTTCTCGAGTTATTCGAGTTGTCCACAGTCAGC of pCyPAwt/pGEX-2TK plasmid with the closed fragment of hrCpA (human recombinant cyclophilin A) gene having a size of 498 base pairs is cloned at XhoI-NdeI sites.

EFFECT: presented strain is high-producing; it requires no complicated clean-up system and can be used in cancer treatment.

2 dwg

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Strain of spore bacteria Bacillus megaterium 501 GR, possessing growth-stimulating effect, is deposited in ARRIAM RAAS under registration number RCAM00875 and can be used as polyfunctional means for plant growing, improving phosphorus feeding of plants, as well as promoting bioremediation of pesticide-polluted soils.

EFFECT: invention makes it possible to increase plant productivity, as well as to protect plants from phytopathogenic fungi and microorganisms, causing diseases of plant roots.

13 tbl

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Halobacterium salinarum RNCIM B-11850 - producer of bacteriorhodopsin is described. The strain of Halobacterium salinarum ST 3 is obtained by multistage selection of halophilic bacteria strain Halobacterium salinarum RNCIM B-10425 without preliminary treatment with chemicals and is deposited under the number RNCIM B-11850.

EFFECT: strain Halobacterium salinarum RNCIM B-11850 exceeds the prototype RNCIM B-10425 in productivity.

2 tbl, 2 ex

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Halobacterium salinarum D96N RNCIM B-11953 - producer of bacteriorhodopsin, significantly surpassing in productivity (biomass and bacteriorhodopsin) as the closest analogue, as well as others selected as controls strains under different conditions of culturing.

EFFECT: increased properties of the strain.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to the strain of Bifidobacterium longum NCIMB 41675, a composition, containing thereof, and a food product, containing the said strain or composition. The claimed strain possesses an ability to induce the production of cytokins and control IL-10:IL-12 ratio is suitable for application in immunomodulation, treatment of an autoimmune disease.

EFFECT: claimed strain, composition and food product possess probiotic properties.

25 cl, 16 dwg, 4 tbl, 8 ex

FIELD: food industry.

SUBSTANCE: invention relates to bakery industry. Saccharomyces chevalieri and/or Saccharomyces cerevisiae var. boulardii yeasts are used for stabilisation of fermentation during bread bakery products manufacture. The dough composition for bakery products contains Saccharomyces chevalieri and Saccharomyces cerevisiae var. Boulardii yeasts, at least one baking improver chosen from baking enzymes, oxidising agents, emulsifiers, fats, reducers and fermentable sugars. The method for production of baked or fried product of the dough composition envisages dough moulding by way of mixing flour, water, yeasts, and, optionally, at least one baking improver chosen from baking enzymes, oxidising agents, emulsifiers, fats, reducers and fermentable sugars and 0.5 - 10 wt % of Saccharomyces chevalieri and Saccharomyces cerevisiae var. Boulardii yeasts, fermentation of the dough composition of bakery products at a temperature of 15 - 45C to produce fermented dough with specific volume equal to at least 1 mg/g, the fermented composition baking and cooling.

EFFECT: fermentation stabilisation.

17 cl, 8 dwg, 1 ex

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