Method for preparation of collagen product

FIELD: pharmacy.

SUBSTANCE: invention refers to processing and cosmetics industris, namely to a method for production of a collagen product with the following characteristics: reception of skins of freshwater fish such as silver carp, carp, grass carp, mirror carp, manual or machine cleaning from scale, muscle tissue, fins and mechanical impurities, washing for 30 minutes in drum type machines with a solution of soap and then in water - to remove residual soap, the rinsed skins are subjected to peroxide and alkaline treatment, for which they are poured with a mixture of hydrogen peroxide with mass concentration of 3% and sodium hydroxide with mass concentration of 3 % and kept at 20-25°C for 1.5-2 hours and liquor ratio of 1:4, the treated skins are washed with flowing water at 8-12°C for 10-15 min, then the skins are poured with citric acid solution with mass concentration of 6% and kept for 6 days, the resulting mass is homogenized, packaged in polyethylene and stored at 0…+4°C for° max. 4 months.

EFFECT: increase of collagen product quality indicators: increase in collagen output, improved organoleptic properties, reduced complexity and cost by reducing the number of process steps and reagents, intensification of the pre-treatment process and raw materials degreasing.

1 tbl, 2 ex

 



 

Same patents:

FIELD: chemistry.

SUBSTANCE: method of processing lake frog includes soak, fleshing, degreasing, fermentative processing, degreasing, washing, liming, partial de-ashing, softening, pickling, tanning, neutralisation, after-tanning, decoration operations. First fermentative processing of frog skin is realised by preparations Pectophoetidin G20x with concentration 2.0-2.5 g/dm3, Celloviridin G20x with concentration 2.0-2.5 g/dm3 and Protosubtilin G3x with concentration 2.2-2.7 g/dm3 with the total liquid coefficient1.6 and temperature 32°C in drum in rotation-stop mode for respectively 5 and 30 min for 90 min. Second fermentative processing - properly softening is carried out after the process of liming with application of Ca(OH)2 with concentration 10.0-13.0 g/dm3 and Na2S with concentration 1.5-2.5 g/dm3 and partial de-ashing to pH=9.0 by proteolytic enzymes: alkaline protease Protosubtilin G3x with concentration 2.0-3.0 g/dm3 for 90 min. Tanning is realised in three stages: at first with potash alums with concentration 10.0-15.0 g/dm3, then by chrome tanning agent with concentration 10.0-13.0 g/dm3 and after laying and neutralisation with Na2CO3 with concentration 4.2-4.9 g/dm3 after tanning is carried out with syntan "Basyntan" with concentration 10.0-18.0 g/dm3.

EFFECT: method of processing improves physical-mechanical characteristics of frog skin to a considerable extent.

1 tbl, 1 ex

FIELD: process engineering.

SUBSTANCE: invention relates to tannery and can be used for production of leather from fish skin. Proposed method comprises processing by organic tanning agent over surfactant. The organic tanning agent represents a compound based on the products of condensation of propylene carbonate, formaldehyde and monoethanolamine and used in amount of 6.0-7.8 wt % of skin weight. Said surfactant represents a water-organic compound based on nonionic surfactants, organic solvent and auxiliary admixtures used in amount of 0.25-0.45 wt % of skin weight. Processing is performed for 6-8 hours. Note here that in two hours after tanning beginning micro talc is added to the solution in amount of 0.5-2.0 wt % of skin weight.

EFFECT: higher quality of leather, expanded range of products.

2 tbl, 3 ex

FIELD: process engineering.

SUBSTANCE: proposed method comprises tanning, dyeing, oiling, drying, sorting semi-finished products into those with deep and shallow surface defects. Additionally, semi-finished products are processed laser liner radiation with wavelength of 1.064-1.075 mcm and power of 1.1-1.5 kW at processing speed of 8-10 m/min. After said additional processing, bottom composition is applied on semi-finished product with shallow defects that comprises, in wt %: 40%-water dispersion of methyl methacrylate copolymer, butyl acrylate, vinyl acetate and methacrylic acid at the ratio of 20:50:25:5, respectively 20-30, penetrator 5-7, and water of up to 100. Composition is applied on surface with deep defects that comprises the following components in wt %: pigment concentrator 7-9, 40%-water dispersion of butyl acrylate, acrylonitrile and methacrylic acid at the ratio of 70:25:5 respectively 35-45, wax emulsion 9-10, 10%-casein 7-8, mix of aminoparaffins containing 1-6 aminogroups and chlorinated paraffins at the ratio of 1:1-4-6, and water of up to 100. Obtained coatings are fixed by composition containing, in wt %: 40%-water dispersion of butyl acrylate and acrylonitrile copolymers at the ratio of 64:36 35-45, wax emulsion 3.5-4.5, 10%-casein 3,5-4,5, mix of aminoparaffins containing 1-6 aminogroups and chlorinated paraffins at the ratio of 1:1 3.5-4.5, and water of up to 100.

EFFECT: higher durability of leather from low-grade raw stock.

3 tbl, 1 dwg

FIELD: process engineering.

SUBSTANCE: fish skin is soaked in solution containing sodium chloride and antiseptic agent at 18-20°C for 4.0-6.0 hours. Then, fish skin is degreased by 0.8-1.0%-solution of enzyme preparation Liporesin "ГЗх" for 1.5-2.0 h at 37-40°C and intermittent mixing. Then, degreased fish skin is treated by 0.3-0.6%-solution of enzyme preparation Protosubtiline "ГЗх" for 1.5-2.0 h at 37-40°C and intermittent mixing. Fish skin treated by Protosubtiline "ГЗх" is flooded by 0.3-0.6%-solution of enzyme preparation Collagenase and treated for 1.5-2.0 h at 37-40°C and intermittent mixing. After every said treatment, fish skin is carefully rinsed by cold running water to remove residues of enzyme ppreraiton. Scales are remove from skin to be fleshed and, then, pickled for 8-12 h at 18-20°C. Now, fish skin is tanned by willow bark broth for 12-20 h at 18-20°C, aromatised, dubbed and dried.

EFFECT: perfected process.

1 dwg, 1 tbl, 3 ex

FIELD: machine building.

SUBSTANCE: collagen containing composition consists of tanned collagen containing waste and products of solution of not tanned collagen. Additionally composition contains a surface active substance. As tanned collagen containing waste there is used tanning powder produced from waste of leather of chrome tannage of shoe industry. This waste is preliminary crumbled in air-dry state to size of fibres to 0.7-0.9 mm. The composition has the following ratio of components, wt %: tanning powder 12-15; products of collagen solution 1.5-2.5; SAS 0.02; water - the rest. The advantage of produced material is simple content of collagen containing composition including mainly collagen containing waste, which facilitates production of material similar to natural leather by chemical contents.

EFFECT: natural and ecologically safe fibrous porous material of increased strength.

2 tbl

FIELD: chemistry.

SUBSTANCE: method involves tanning a semi-finished product with a chrome tanning agent, neutralisation, washing, dyeing and tanning with organic tanning agents twice. The first tanning is carried out with a polymeric tanning agent, while the second is carried out with a mixture of synthetic and vegetable tanning agents. Hydrophobicising greasing, treatment with an organic acid and re-tanning with a chrome tanning agent are then carried out. Additional washing is carried out before neutralisation. Dyeing is carried out after the second tanning. Neutralisation, tanning twice and dyeing are carried out at water-to-goods ratio of 0.5-0.7. Hydrophobicising greasing is carried out in the presence of a 20% solution of the product of reacting amino alcohol with fatty acids of vegetable oils of the C12-C22 fraction and boric acid in molar ratio of 2:1:1 in mineral oil in amount of 0.5-1.5% of the weight of the semi-finished product.

EFFECT: good hygienic properties of hydrophobic leather and prolonged fungus resistance of the leather in high humidity and temperature conditions.

2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: method involves chrome tanning and post-tanning a semi-finished product through chrome tanning in the presence of a product of reaction of 3-aminopropyltriethoxysilane and glycidylmethacrylate in molar ratio of 1:2 and consumption of the chrome tanning agent and reaction product of 3-4% and 2-3% of the weight of the semi-finished product respectively. Neutralisation and hydrophobisation with a polymer tanning agent are then carried out for 30-40 minutes with consumption of the tanning agent equal to 8-10% of the weight of the semi-finished product. After that dyeing is carried out in the presence of a synthetic tanning agent in amount of 3-4% of the weight of the semi-finished product. Post-tanning is carried out with a plant-material tanning agent and the semi-finished product is also treated for 20-30 minutes with a product of reacting hexamethylenediamine with phosphoric acid and urea in the presence of glycerin in molar ratio of 1:3:3:0.5 respectively, in amount of 3-4% of the weight of the semi-finished product and for 20-30 minutes with organic acid in amount of 0.5-1.0% of the weight of the semi-finished product. Further, the semi-finished product undergoes greasing and re-treated for 40-50 minutes with a product of reacting hexamethylenediamine with phosphoric acid and urea in the presence of glycerin in molar ratio of 1:3:3:0.5 and consumption of 7-8% of the weight of the semi-finished product, volume ratio of 0.8-1.0 and temperature of 20-22°C. Hydrophobisation with a polymer tanning agent, dyeing in the presence of a synthetic tanning agent, post-tanning with a plant-material tanning agent, further treatment with a product of reacting hexamethylenediamine with phoshoric acid and urea in the presence of glycerin in the said molar ratio and with organic acid is carried out in one bath. Separation and drying are carried out after all operations.

EFFECT: higher water-, fungus- and heat-resistance of leather under high moisture and temperature conditions, and wide range of special types of leather.

2 cl, 3 tbl, 5 ex

FIELD: leather industry; treatment of collagen-containing raw material of fish skin formed in the course of dressing.

SUBSTANCE: waste of fish skin treatment process in leather and fishing industries is frozen, ground in pieces of 20x20 mm, defrosted by washing with tap water which is changed for two or three times; washing is continued for 2 hours at temperature of 18-20 C. Then pieces of waste are treated with solution containing 2-4-% CH3OOO at volume ratio equal to 5 and are kept in solution at regular mixing for 12 hours at temperature of 18-20 C. After treatment, solution is drained. Collagen thus obtained is washed with running water for 30 minutes at temperature of 18-20 C. After washing, collagen is dissolved in alkaline salt bath for 4 hours: 2-3-% NaOH and 6-7-% Na2SO4 at volume ratio equal to 5 and temperature of 18-20 C. Then homogenization is carried out through capron cloth. In the course of homogenization, undissolved scale is removed from capron cloth.

EFFECT: enhanced dissolving of collagen; reduced duration of technological cycles; reduced consumption of reagents; low contamination of waste water.

3 ex

FIELD: polymer materials.

SUBSTANCE: invention relates to technology of synthetic fibers, in particular to producing composite bicomponent fibers of the islands-in-sea type and deerskin-type materials, in blends and stripped. In its island, polyethylene-terephthalate component, fibers comprise diol comonomer of formula I and formula II and, while its sea component is made from polyester copolymer easily soluble in alkali. Linear density of fiber varies from 0.001 to 0.5 denier. Deerskin-type materials is made completely or partly from ultrafine fibers with specified density and including indicated diol comonomer of formula I and II. Blended material and stripped material are manufactured using some parts of indicated islands-in-sea bicomponent fiber. Lower are formulas I and II: wherein R1 and R2 are С13-alkyl groups and X aliphatic ring with 5-8 carbon atoms.

EFFECT: enhanced color saturation.

14 cl, 3 dwg, 1 tbl

FIELD: leather industry.

SUBSTANCE: material and manufacturing procedure relate to macroporous collagen-containing materials accessible via processing of collagen-containing slurries showing complex of physico-mechanical and hygienic properties usually being characteristic of leather made from animal skins and which can be used in leather industry. Leather-like macroporous material contains collagen, tanned leather fibers, and polymer possessing capability of joining collagen fibers into one indivisible structure, amount of said polymer corresponding to 3-30% based on the summary weight of collagen and leather fibers. Method for manufacturing leather-like macroporous material envisages use of freezing followed by vacuum drying to residual moisture no higher than 12%, ageing at room temperature for 1 day period, treatment with aqueous polymer dispersion providing joining of collagen fibers into indivisible structure, and additional vacuum drying at temperature no lower than polymer film-formation temperature and no higher than temperature of the beginning of thermal shrinkage of collagen fibers.

EFFECT: improved physico-mechanical properties of leather-like macroporous sheet material and simplified manufacturing procedure.

4 cl, 4 ex

FIELD: biotechnologies.

SUBSTANCE: inventions refer to biotechnology and can be used for collagen substrate obtainment. The method involves hide trimming, clipping and scouring. Raw hide is processed with peroxide alkali solution containing 1% to 10% of sodium hydroxide and 0.05% to 0.8% of hydrogen peroxide at 5°C to 20°C. Raw hide is degreased at inner side till fatty substance content is reduced to 0.05-1%, and flushed in flowing water to obtain neutral pH. Raw hide is aged for 6-24 hours at -20°C to -30°C and ground at -10°C - -20°C to a particle size of 2-5 mm. To obtain dry powder collagen substance, ground raw hide is dehydrated by sublimation to moisture level of 1-10% with further temperature rise to 20-40°C and hide milling to powder, thus obtaining the end product. To obtain liquid collagen substance, ground raw hide is treated with food acids and filtered, thus obtaining the end product.

EFFECT: improved quality of product obtained, reduced process duration.

3 cl, 2 ex

FIELD: chemistry.

SUBSTANCE: method involves soaking, first and second defatting in aqueous solution of an anionic surfactant and a preparation containing an oxyethylated compound, polyvinyl alcohol and a saturated monoatomic C1-C3 alcohol in ratio of 60:20:20, respectively; pickling in the presence of the preparation in amount of 2.0-3.0 g/l; tanning-oiling is carried out in a treatment solution containing sodium chloride; the oiling agent and chrome tanning agent are used in amount of 1.0-1.4 g/l per content of chromium oxide, in which before adding the chrome tanning agent, aluminium hydroxochloride and sodium formate are further added in amount of 10.0-14.0 g/l and 2.0-3.0 g/l, respectively. First defatting is carried out with amount of the anionic surfactant and the preparation of 2.0-3.0 g/l each; second defatting is carried out with amount of the anionic surfactant and the preparation of 3.0-4.5 g/l and 1.0-1.5 g/l, respectively; aluminium hydroxochloride is added in two equal portions with an interval of 50-60 min; the chrome tanning agent is added 4.0-5.0 hours after adding sodium formate.

EFFECT: improved quality of a half-finished product made from sheepskin owing to high strength thereof, reduced contamination of factory sewage with chromium compounds and high environmental safety of fur production.

5 cl, 1 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: in order to obtain quality collagen dissolution products, rawhide is treated with a solution in form of a lactic acid composition having titratable acidity of 250-300°T, concentration of lactic acid of at least 25-30 g/dm3 and medium active response of not more than 4.0. The process is carried out at temperature of 24±2°C, float ratio=1 and alternating mechanical action. Dissolution has duration of 11-13 days. At the end of dissolution, the samples are almost transparent, loose, swollen pieces of skin which easily break up under slight mechanical action.

EFFECT: improved properties of the product.

3 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: method of treating leather material, which involves successive two-step soaking with a surfactant-based composition, liming, deliming, softening, pickling and tanning, wherein at the first soaking step, at the second soaking step, as well as at the liming step, the composition used contains surfactants in form of a product of condensation of float tar with triethanolamine, taken in molar ratio (0.8-1.2):1.0, sodium alkylbenzene sulphonate and neonol with average degree oxyethylation 12, water and isopropyl alcohol, also employs sodium carbonate, sodium sulphide and calcium hydroxide depending on the step. All components are taken in a defined ratio.

EFFECT: invention improves quality of material, reduces concentration of toxic sulphides in waste water, enables to use domestically produced chemicals, including oleic acid production wastes, thereby reducing the cost of the process of treating leather material.

2 tbl, 3 ex

FIELD: process engineering.

SUBSTANCE: fish skin is soaked in solution containing sodium chloride and antiseptic agent at 18-20°C for 4.0-6.0 hours. Then, fish skin is degreased by 0.8-1.0%-solution of enzyme preparation Liporesin "ГЗх" for 1.5-2.0 h at 37-40°C and intermittent mixing. Then, degreased fish skin is treated by 0.3-0.6%-solution of enzyme preparation Protosubtiline "ГЗх" for 1.5-2.0 h at 37-40°C and intermittent mixing. Fish skin treated by Protosubtiline "ГЗх" is flooded by 0.3-0.6%-solution of enzyme preparation Collagenase and treated for 1.5-2.0 h at 37-40°C and intermittent mixing. After every said treatment, fish skin is carefully rinsed by cold running water to remove residues of enzyme ppreraiton. Scales are remove from skin to be fleshed and, then, pickled for 8-12 h at 18-20°C. Now, fish skin is tanned by willow bark broth for 12-20 h at 18-20°C, aromatised, dubbed and dried.

EFFECT: perfected process.

1 dwg, 1 tbl, 3 ex

FIELD: process engineering.

SUBSTANCE: oil, fat or polymer is applied on leather in amount of less that 20%, preferably, less than 15%, particularly preferably less than 10%, per leather weight. Then leather is treated by compressed air. Carbon dioxide is used as compressed air, treatment if carried out at pressure below 70 bar and temperature below 25°C. Leather is processed by compressed air unless leather weight is at least 1% smaller than its weight prior to applying oil, fat or polymer.

EFFECT: improved leather structure due to increased non-blocking property of molecules.

1 ex

FIELD: technological processes.

SUBSTANCE: method includes treatment of fur stock with pickling solution, containing lactic acid and sodium chloride. Acid used is represented by combined whey with lactic acid concentration from 15 to 25 g/dm3. Process of pickling is done by dipping method with duration of 16 hr, fatty acids = 10, temperature of solution is 35°C, at periodical mechanical action. After treatment ageing is carried out.

EFFECT: increased strength, softness, plasticity of skin tissue in fur semi-finished piece and reduced aggressive effect at environment.

3 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: method involves degreasing sheepskin-fur raw material and can be used in fur production during degreasing of sheepskin-fur coat and fur raw material. Processing of the raw material is carried out in a degreasing bath containing a bacterial suspension, which has both lipolytic 50-60 units/gram and proteolytic 8-10 units/gram activity, with the total product of life activity of the microorganisms at a value of 5-6 g/dm3, synthetic surface-active substance - 0.5-1.0 g/dm3, catalase-positive procariotic culture of genus Erwinia sp. in the quantity 109-1010 cells/cm3 in the degreasing bath hydrogen peroxide is additionally added in the quantity 0.1-1.0 g/dm3, preliminarily dissolved in a tenfold amount of piped water. Processing is carried out at a temperature of 40±2°C, for 60 min., with some mechanical action from time to time and liquid crystallisation=10.

EFFECT: increase in quality of degreasing sheepskin and reduction in the level of toxic sewage production.

4 tbl, 4 ex

FIELD: material processing.

SUBSTANCE: method provides degreasing and/or drying of animals' skins. According to method, skins, being subject to degreasing and/or drying, are brought in contact with one or more extractants. At least one dissolvent, selected from group consisting of dimethyl ether, methylal, dioxolane, diethyl ether and methyl ethyl ketone, is used as single dissolvent. Sheep, cows', goat's or pig's skins are subject to degreasing and drying. Process is carried out in stator or rotor reactor under pressure of 1-12 bar and at temperature between 5°C and 40°C.

EFFECT: improved efficiency of cleaning; improved ecological safety of process.

14 cl, 1 dwg, 4 ex

FIELD: leather industry.

SUBSTANCE: method involves pickling leather depilated hide with sodium chloride and sulfuric acid, addition of chrome tanning agent to the same bath in its consumption 0.5-0.6% of depilated hide mass as measured for chrome oxide, and chrome-plating is carried out for 2-4 h. Aliphatic dialdehyde is added to same bath in the concentration 3-6% of depilated hide mass, and treatment is carried out for 2-3 h wherein glyoxal or glutaraldehyde is used as dialdehyde. Invention provides enhancing thermal stability and strength properties of leather and can be used in tanning leathers for different designation and assortment.

EFFECT: improved tanning method.

2 cl, 1 tbl, 9 ex

FIELD: chemistry.

SUBSTANCE: invention relates to leather industry and can be used in treating raw wastes to obtain an ecologically clean protein hydrolysate and for use in medicine, when producing cosmetic products and as a cattle feed additive. The method of producing a protein hydrolysate involves washing and treating raw wastes from leather production, the treatment being carried out in two steps: at the first step the wastes are treated with an extractive solvent selected from esters at boiling point of the solvent, followed by drying; at the second step the wastes are treated with unsaturated steam at pressure of 1 atm and temperature of 100°C with weight concentration of the steam in water of 5-10% until full extraction of collagen.

EFFECT: obtaining an ecologically clean protein hydrolysate with quantitative output and wider field of practical use thereof.

1 tbl

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