Trametes hirsuta strain - producer of ethanol
SUBSTANCE: Trametes hirsuta MT-24.24 basidiomycete strain has the ability to produce ethanol. Trametes hirsuta basidiomycete strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM F-1288 and can be used in medicine and food industry.
EFFECT: invention allows to obtain high quality alcohol containing no impurities such as acetone and isopropanol.
1 tbl, 5 ex
SUBSTANCE: invention relates to the hydrolysis industry, particularly methods of removing acetone-butyl-alcohol fermentation inhibitors from hydrolysates of lignocellulose material, and can be used in preparing culture media for producing biethanol, biobutanol and acetone. The method includes treating hydrolysates with a group of microorganisms, wherein the consortium of microorganisms used is active sludge from sewage treatment facilities which is pre-adapted to a growth substrate based on substances which inhibit acetone-butyl-alcohol fermentation. The method employs active sludge from urban sewage treatment facilities, the bacterial component of which is represented by Pseudomonas (64%), Bacillus (18%), Zooglea (7%), Micrococcus (5%), Chromobacterium (3%), Acinetobacter (2%) and Citrobacter (1%) bacteria. The method also employs active sludge from sewage treatment facilities of pig-breeding farms, the bacterial component of which is represented by Nocardia (35%), Rhodococcus (28%), Micrococcus (18%), Pseudomonas (13%) and Bacillus (6%) bacteria.
EFFECT: invention eliminates inhibiting factors in the process of fermentation of hydrolysates of lignocellulose material, enables to avoid corrosion of the hydrolysis equipment and enables to optimise the microbial culture.
3 cl, 4 ex
SUBSTANCE: invention relates to the field of biotechnology. Claimed is a transformed microorganism - yeasts Saccharomyces for obtaining ethanol, where the said microorganism is transformed with a nucleotide sequence, coding xylose isomerise, and the said microorganism is transformed by a nucleotide sequence, coding xylulokinase, or the said microorganism is transformed with a promoter, capable of increasing the expression of endogenic xylulokinase. The said microorganism is capable of a higher xylose isomerise activity; higher growth rate in a growth medium or on a growth medium, which contains xylose; faster xylose metabolism; and/or faster ethanol production when grown in anaerobic conditions on xylose as a carbon source than an equivalent microorganism before transformation. An inoculant and culture medium, containing the said transformed yeasts and xylose or a source of xylose, are described. Claimed is a method of obtaining the said transformed microorganism, including a stage of the microorganism transformation with the xylose isomerise-coding nucleotide sequence, or the xylulokinase-coding nucleotide sequence, or the promoter, capable of increasing the expression of endogenic xylulokinasse. Described is a method of fermentation, including the cultivation of the said microorganism in the culture medium, which contains xylose or xylose source. Claimed is a method of obtaining ethanol-containing biofuel, where the said method includes a stage cultivation of the microorganism in accordance with the claimed invention in the culture medium, which contains xylose or source of xylose. Application of the yeasts in accordance with the claimed invention for obtaining ethanol is described.
EFFECT: invention makes it possible to obtain ethanol by means of the said transformant in a larger amount in comparison with the equivalent microorganism before transformation, on the xylose-containing medium.
11 cl, 7 dwg, 1 tbl, 7 ex
SUBSTANCE: invention relates to distillation industry in particular to method of beer heating by spent wash heat. The method includes beer supply to tube side of the shell-tube heat exchanger, at that spent wash is sent to the tube bundles of the other heat exchanger, and shell side is filled with liquid heating medium (luther, process water, rectified spirit) that is continuously transferred by the pump from shell side of one heat exchanger to the shell side of the other ensuring continuous circulation of the heating medium between two heat exchangers, and heat exchange in system spent wash-heating medium-beer.
EFFECT: method excludes blockage and necessity of shell side cleaning of heat exchangers.
1 dwg, 1 tbl, 2 ex
SUBSTANCE: method provides producing ethanol by ethanol extraction from fermentable wort in a distillation column, wash distillate cleaning from head and intermediate impurities in an epuration column operating by deep hydroselection, epurate rectification in an alcohol column, impurity recovery in a final purification column operating in the mode of re-epuration and purification of fractions containing head impurities and methanol in a head impurity concentration column. The alcohol column, final purification column and head impurity concentration column operate in vacuum and are heated by secondary alcohol water steam from other columns; between the alcohol column and final purification column, there is an ion exchange reaction for recovering impurities having the negative effect on the organoleptic parameters of the end product.
EFFECT: invention enables improving the organoleptic parameters of ethanol, improving its qualities and reducing the production-related energy consumtion.
SUBSTANCE: group of inventions relates to biochemistry. Disclosed is a method for degradation of pretreated lignocellulose biomass, as well as a method for degradation of lignocellulose biomass. The method for degradation of pretreated lignocellulose biomass includes cultivating a microorganism, treating the microorganism and/or a microorganism-rich suspension and enzymatic hydrolysis of the pretreated lignocellulose biomass. The method for degradation of lignocellulose biomass includes steps of physical-chemical pretreatment of the lignocellulose biomass to obtain a pretreated slurry, separating the pretreated slurry into part A and part B, incorporating part A into a raw growth medium to obtain a final growth medium and cultivating a microorganism, capable of producing an enzyme, treating the microorganism and/or microorganism-rich suspension, enzymatic hydrolysis of the biomass of part B.
EFFECT: invention provides faster hydrolysis kinetics and increases monomeric sugars.
32 cl, 2 dwg, 4 ex
SUBSTANCE: invention relates to methods of carbohydrate feedstock preserving against microorganisms. Contacting the carbohydrate feedstock in a unit operation is executed. The unit operation involves the carbohydrate feedstock storage or transportation with stabilised chlorine dioxide at a pH of at least 2.6 over a period of at least one month before its use in the fermentation process.
EFFECT: carbohydrate concentration of at least 1% by the feedstock weight, the amount of added stabilised chlorine dioxide ranges from 10 to 10000 mg/kg, as ClO2 based on the total feedstock weight, the microbial population of the preserved feedstock does not undergo an increase of more than 1 log10 CFU/ml or 1 log10 CFU/g for at least one month.
11 cl, 2 tbl, 2 ex
SUBSTANCE: invention relates to method of obtaining ethyl alcohol and protein product from grain raw material. Method includes milling grain raw material, its extrusion and fermentative hydrolysis in one extrusion-hydrolytic installation with supply of water and enzyme preparations, saccharification and alcoholic fermentation. Saccharified wort with content of soluble dry substances 33% is obtained in extrusion-hydrolytic installation with 1.5 h exposure at 58°C. Applied are: α-amilase 3 units/g of starch, BrewZyme BGX 0.5 unit KS/g of raw material, glucoamilase 12 units GlS/g, neutral protease 0.15 units PS/g of raw material. Obtained wort is separated into liquid and solid fractions in centrifugal field. Solid deposit is subjected to first washing with water with hydromodulus 1:1, centrifuged and washing sugar-containing waters are returned into clarified wort, diluting it to 21% DS in this way. After that acid protease in amount 0.25 units PS/g of raw material is added, exposed for 60 minutes at 55°C and directed to fermentation, with solid deposit being subjected to second washing with water with hydromidulus 1:1, after that, centrifuged with separation of purified protein. Washing sugar-containing waters are returned to extruder-hydrolyser for the following fermentative hydrolysis.
EFFECT: method makes it possible to reduce loss of grain raw material in realisation of the process.
1 dwg, 1 ex
SUBSTANCE: invention relates to biotechnology and a vector, a host cell containing a vector, a genetically modified microorganism Clostridium thermocellum, a method of producing said microorganism and a method of converting lignocellulose biomass to ethanol. The present vector is designed for acetate kinase gene knockout in Clostridium thermocellum and has nucleotide sequence SEQ ID NO:1 or SEQ ID NO:2. The present genetically modified microorganism is transformed by the nucleotide sequence SEQ ID NO:1 or SEQ ID NO:2, and can also further contain a non-native gene which provides the capacity to metabolise pentose sugar, hydrolyse xylan or is involved in metabolic production of ethanol. The present method of converting lignocellulose biomass to ethanol includes bringing the biomass into contact with said genetically modified microorganism.
EFFECT: invention enables to obtain a larger amount of ethanol.
17 cl, 53 dwg, 6 tbl, 10 ex
SUBSTANCE: method includes distillation of wash in distillation-epuration column with condensation of part of epurated water-alcohol vapour in additional wash heater, supply of water-alcohol from main wash heater into boiler of preliminary purification column and supply of condensate from condenser of carbon dioxide separator into middle zone of said column, concentration of main admixtures on said column plates and in its dephlegmator and withdrawal of fraction of main admixtures from condenser of preliminary purification column. Bottom liquid of said column with fraction of fusel alcohol from rectification column and hydroselection water is supplied onto plate of epuration column feeding. Condensate of epurated water-alcohol vapour from additional wash heater of distillation-epuration column is supplied onto lower plate feeding distillation column of main and tailing fractions. Fraction of purified from admixtures ethyl alcohol from liquid phase of plates from middle zone of distillation column of main and tailing fractions is supplied onto plate of rectification column feeding. Fractions from condensers of rectification column, final purification column and liquid from alcohol trap of pure distillates are supplied onto upper plate feeding distillation column of main and tailing fractions.
EFFECT: invention makes it possible to increase alcohol quality and increase its output.
1 dwg, 2 ex, 1 tbl
SUBSTANCE: group of inventions relates to control of microbial contamination in the process of alcohol fermentation. Claimed is an antimicrobial composition, including from 1 wt % to 5 wt % of an antimicrobial agent of a guanidine family, representing poly(hexamethylbiguanide); from 0.05 wt % to 0.5 wt % of antibiotic; and from approximately 94.5 wt % to approximately 98.5 wt % of a surface-active substance. The claimed composition is intended for control of contaminations with yeasts of a wild type, Lactobacilli and bacterial microbiota, contained in a must for the fermentation, obtaining the effect of deflocculation in the said must and for the protection of fermenting yeasts against competition with contaminants for sugar, contained in the must for the fermentation. A method of control of microbial contamination in the process of alcohol fermentation includes the addition of the said composition to the must.
EFFECT: group of inventions provides the effective reduction of a quantity of contaminant microbiota without an influence on the fermenting yeasts.
5 cl, 1 tbl
SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.
EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.
4 tbl, 3 ex
SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.
EFFECT: invention makes it possible to increase the output of citric acid.
2 tbl, 6 ex
SUBSTANCE: strain Aspergillus oryzae 12-84, having a high level of synthesis of the complex of proteases and peptidases, nucleases, chitinase, β-glucanase, mannanase and α-amylase, is deposited in State Scientific Institution of All-Russian Scientific Research Institute of Agricultural Microbiology of the Russian Agricultural Academy under the registration number Aspergillus oryzae RCAM01134. The strain may be used to produce complex enzyme preparations with their subsequent application for hydrolysis of raw materials in the preparation of biocorrectors of food and feed, amino acid additives and biologically active additives with functional properties.
EFFECT: invention enables to increase the yield of proteolytic, nuclease, chitinase, β-glucanase, mannanase and α-amylase activities.
2 tbl, 2 ex
SUBSTANCE: invention relates to biotechnology and specifically to a fermentation medium and a method of producing recombinant proteins using said medium. A fermentation medium for producing recombinant proteins selected from a group including GM-CSF, streptokinase and lipase, using microorganisms selected from a group including: E. Coli, Streptomyces sp. and Rhizomucor sp., is characterised by keeping concentration of urea or derivatives thereof in the range of 0.5 g/l to 2 g/l. The medium contains, per litre of water, basic salts in the following amounts: orthophosphoric acid (85%) 2.67-133.5 ml, calcium sulphate 0.093-4.65 g, potassium sulphate 1.82-91 g, magnesium sulphate-7H2O 1.49-74.5 g, potassium hydroxide 0.413-20.65 g, glycerine 4-200 g. The medium contains, per litre of water, trace elements in the following amounts: copper sulphate-5H2O 0.6-30 g, sodium iodide 0.008-0.4 g, manganese sulphate-H2O 0.3-15 g, sodium molybdate-H2O 0.02-1 g, boric acid 0.002-0.1 g, cobalt chloride 0.05-2.5 g, zinc chloride 2-100 g, iron (II) sulphate-7H2O 6.5-325 g, biotin 0.02-1 g, sulphuric acid 0.5-25 ml.
EFFECT: invention increases the output of end products.
6 cl, 27 dwg, 3 tbl, 16 ex
SUBSTANCE: strain of micromycete Aspergillus foetidus 379-K-5-1 is proposed, producing a complex of pectinases, β-glucanase, xylanase, cellulase, chitinase, mannanase and protease for the destruction of polysaccharides of a plant and microbial material. The strain is deposited in the Departmental collection of useful microorganisms for agricultural purposes of the Russian Academy of Agricultural Science (RCAM) of the State Scientific Institution of the Russian National Research Institute for Agricultural Microbiology under the registration number of RCAM 01136. The advantage of the new strain is to obtain the more active complex of enzymes catalyzing the hydrolysis of polysaccharides, as well as endopolygalacturonase and pectinesterase. The strain Aspergillus foetidus RCAM 01136 is produced using efficient methods of selection and mutagenesis using UV irradiation and nitrosoguanidine from the known strain.
EFFECT: invention enables to expand the range of the enzymes obtained, used for the destruction of polysaccharides.
SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants against invasive and perennial weed vegetation of class Dicotyledones. The suspension is used on the basis of vegetative mycelium of phytopathogenic micromycetes with chemical herbicides in sublethal doses. The plants are treated by spraying an aqueous solution of the herbicide with the active substance of glyphosate, and suspension of mycelium of foma-like fungi. Treatment is carried out at all stages of plant development, first with herbicide at the flow rate by glyphosate active substance of 0.02-0.32 kg/ha, then by a suspension based on mycelium fragments of foma-like fungi in a concentration of from 25 to 100 g/l. The consumption rate of the working fluid is from 50 to 300 l/ha. The proposed method enables to reduce 1.5-10 times or more the chemical load on the ecosystem, 1.5-4 times the infectious load (concentration of phytopathogenic fungi mycelium).
EFFECT: method can be applied at all stages of plant development, without requiring additional protection of the cultivated crop.
10 tbl, 5 ex
SUBSTANCE: invention relates to method of obtaining 9-decen-2-one. Method includes cultivation of said mould, belonging to family Aspergillaceae or Mortierellaceae, addition of undecylenic acid as substrate in fermentation medium with consumption from 0.1 to 0.9 g/l/hour in presence of oil, substrate bioconversion in 9-decen-2-one, its extraction and purification. As oil, used is oil, selected from the group, containing traditional food oils or triglycerides, which contain short-chained fatty acids, or white oils.
EFFECT: invention makes it possible to obtain 9-decen-2-one with output 8-16,5 g/l and high degree of purification 99%.
13 cl, 1 dwg, 9 ex
SUBSTANCE: strain Trametes versicolor is proposed, used for production of antifungal agents against fungi of the genus Penicillium. The strain is deposited in the RCIM under the number F-1024.
EFFECT: strain has high chitinase and fungicidal activity.
4 dwg, 1 tbl, 5 ex
SUBSTANCE: invention relates to biotechnology and can be used in agriculture. The Trichoderma harzianum Rifai strain is deposited in the Russian National Collection of Industrial Microorganisms under registration number VKPM F-180. The strain is capable of producing L-lysine-alpha-oxidase and can be used particularly as an inhibitor of cucurbit bacterial spot caused by Acidovorax citrulli bacteria.
EFFECT: invention reduces loss of cucurbit crop.
SUBSTANCE: method for obtaining proteinase - protein C activator of blood plasma provides for solid-phase cultivation of fungus strain Aspergillus ochraceus BKM F-4104D on a substrate. The substrate contains the following components at a certain ratio: a solid substance or a carrier, glucose, amylum, hydrolysate of fish flour, peptone, NaCl, KH2PO4, MgSO4·7H2O, and water. As a solid substance or a carrier, vermiculite, polyurethane foam, silica gel or wheat middlings are used. Proteinase activity is up to 300 U/ml.
EFFECT: invention allows obtaining a target product with improved activity.
5 tbl, 15 ex
FIELD: biotechnology, in particular reagent for structural protein hydrolysis.
SUBSTANCE: method for production of protheolytic reagent includes cultivation of producer strain selected from dermatophyte of species Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton equinum, Microsporum canis, Microsporum gypseum, preferably from fungus strains capable to produce complex of structural protein proteinases (scleroproteases) with total protheolytic activity at least 0.7 U/mg including collagenase 0.1-4.5 U/mg; keratinase 0.1-0.5- U/mg; elastase 0.5-1.9 U/mg. Cultivation is carried out, for example, in wort agar-agar or in wort broth for 20-24 days.
EFFECT: scleroproteases with improved specific activity; method for protheolytic cleavage of specific substrates (scleroproteins) with increased rate and depth.
2 dwg, 12 ex