Method of adaptation of regenerated strawberry plants

FIELD: agriculture.

SUBSTANCE: invention is a method of adapting the regenerated plants of strawberries, including the adaptation phase where the regenerated plants of strawberry large-fruited in the period of adaptation to moisturize three times during the period at regular intervals freshly prepared aqueous suspension of silicon-mechanocomposite based on rice husk and green tea prepared by mixing siliceous mechanocomposite and room temperature water in a concentration of 3 g/l and subsequent infusion for 1 hour at room temperature, and in between is moistened with distilled water.

EFFECT: invention can successfully acclimate strawberries macrocarpa through feeding microplants stage adaptation siliceous mechanocomposites based on rice husk and green tea.

1 tbl

 



 

Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to methods of producing a plant with higher drought and salt tolerance compared to the wild-type plant by reducing expression/function of the protein transcription factor in the plant. The invention also relates to a plant with high drought and salt tolerance obtained using said method.

EFFECT: invention enables to efficiently obtain a plant with high drought and salt tolerance compared to the wild-type plant.

6 cl, 6 dwg, 1 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the field of chemistry, in particular to the transgenic plant Brassica juncea, in seeds of which the endogenic content of fatty acids includes from approximately 70 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid, where the plant contains fad.2 and fad.3 allele, coding a mutant protein of delta-12-desaturases of fatty acids, which has an amino acid sequence, selected from the group, consisting of SEQ ID NO:5-7, as well as to the seed of the said plant and to oil of the said seed, the content of fatty acids of which includes from approximately 70.0 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid, which in the rest has properties of the Brassica juncea seeds.

EFFECT: invention makes it possible to effectively obtain oil of the Brassica juncea seeds, in which the content of fatty acids includes from approximately 70 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid.

7 cl, 4 dwg, 6 ex

FIELD: chemistry.

SUBSTANCE: invention refers to biochemistry, particularly to a method for weed control near sunflower plants. The method involves applying an effective amount of any of the herbicides specified in a group consisting of imidazolinone herbicide, sulphonylurea herbicide, triazolopyrimidine herbicide, pyrimidinyloxybenzoate herbicide and their mixture, on weeds and sunflower plants. The above sunflower plant contains in its genome at least one copy of at least acetohydroxyacid synthase (AHAS) gene coding a herbicide-resistance protein having an amino acid sequence presented by SEQ ID NO:2.

EFFECT: invention enables controlling weeds near sunflower plants effectively.

9 cl, 13 dwg, 14 tbl

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and virology. What is presented is a method for producing viral-like influenza virus particles (IVP) in a plant or a part thereof. The method involves the expression of a new influenza virus protein HA in plants and purification thereof. The invention also aims at IVPs containing the influenza virus protein HA and herbal lipids. The invention also refers to nucleic acids coding an improved influenza virus HA, as well as to vectors. The IVPs can be used in developing the influenza vaccines or for the treatment of existing vaccines.

EFFECT: presented group of inventions can be used in medicine.

18 cl, 44 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to biochemistry, particularly to a gene expression construct containing: (a) an expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, b) a polylinker for facilitating a gene insertion into a gene expression system. What is disclosed is a gene expression vector containing the above construct and heterologous gene. What is also described is the gene expression vector containing: (a) the expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, (b) the heterologous gene, which codes a protein of interest, as well as a method for expressing the protein of interest with using it. A method for increasing translation enhancement activity of the heterologous protein of interest from the heterologous gene coding the above protein, a method for translation enhancement of the heterologous protein of interest from the heterologous gene or a heterologous open reading frame coding the above protein are disclosed. There are also described a host organism transformed by the above vector, as well as a method for producing the protein of interest, involving collection of the host organism tissue.

EFFECT: invention enables enhancing translation of the heterologous protein of interest from the heterologous gene.

26 cl, 15 dwg, 4 tbl, 6 ex

FIELD: biotechnology.

SUBSTANCE: expression cassette, the recombinant vector, the seed and the plant are disclosed, comprising the said isolated nucleic acid molecule, as well as the host cell and the plant comprising the said expression cassette. The invention also relates to the use of the said fusion protein in the insecticidal composition and the method of control of European corn worm, and also the method for its preparation. Also the method of production of a transgenic plant is disclosed, which is resistant to European corn worm, with use of the said fusion protein.

EFFECT: invention enables to obtain plants resistant to European corn worm.

39 cl, 8 dwg, 9 tbl, 45 ex

FIELD: agriculture.

SUBSTANCE: invention relates to a method of weed destruction, providing the step of applying the herbicide of PPO-inhibitory type on the cultivated part of the plant, where the said plant is resistant to the said herbicide and expresses the protein with the ability to convert the said herbicide into the herbicide with lower herbicidal activity. The method of evaluation of resistance of the plant cell or the plant to herbicide of PPO-inhibitory type is disclosed, comprising bringing into contact of the herbicide of PPO-inhibitory type with the plant cell or the plant into which DNA is introduced and expressed, which encodes the protein capable to convert the said herbicide into the herbicide with lower herbicidal activity, and also the method of intake of plant cell or plant resistant to the herbicide of PPO-inhibitory type, providing the step of intake of the plant cell or plant based on the aforesaid method for evaluating the stability. Also the plant cell, the plant, the cell culture of plant are disclosed, resistant to herbicide of PPO-inhibitory type, as well as the method of treating the herbicide of PPO-inhibitory type and use of the protein or DNA encoding the said protein for treatment of herbicide of PPO-inhibitory type, where the protein is capable to convert the aforesaid herbicide into the herbicide with lower herbicidal activity.

EFFECT: invention enables to destroy selectively only the weeds on the cultivated part of the plant.

10 cl, 66 dwg, 35 tbl, 75 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to a plant, having high resistance to an AHAS-inhibiting herbicide, which includes at least one Shiloh-8 IMI nucleic acid, parts thereof, a plant cell and seeds. Described is a nucleic acid which encodes a polypeptide which increases herbicide resistance of a plant. Disclosed are an expression cassette and a plant transformation vector which include said nucleic acid. Described are methods of controlling weeds growing near a plant having high resistance to an AHAS-inhibiting herbicide. Disclosed is a method of producing a plant having high resistance to an AHAS-inhibiting herbicide, as well as a method of increasing AHAS activity in a plant. Described is a method of selecting a cell transformed by a vector containing IMI nucleic acid. Also disclosed is a method of increasing resistance to an AHAS-inhibiting herbicide and a weed control method which includes treatment with an AHAS-inhibiting herbicide.

EFFECT: invention enables to obtain a plant which is resistant to an AHAS-inhibiting herbicide, which provides effective control of weeds growing near said plant.

57 cl, 3 dwg, 5 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: leaf explants isolated from the thirty-day aseptic plants of initial varieties grown in 1 l containers, are placed in Petri dishes with the liquid medium of a certain composition and preliminary incubated, co-cultivated and cultivated on nutrient media of a certain composition. When appearance of regenerants of the size at least 10 mm they are cut and transferred to the nutrient media of a certain composition. The first stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, by culturing the cut regenerants on MS medium with 50 ml/L kanamycin sulfate for 30-45 days at 22-25°C and illumination of 6000-10000 lux at 16-hour photoperiod followed by selection of rooted regenerants of potatoes with green leaves and stems. The second stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, including checking of rooted plants by PCR method for the presence of target DNA and reproduction of regenerants with confirmed PCR target DNA insert by micrografting.

EFFECT: use of claimed method enables to obtain potato forms resistant to Phytophthora and Alternaria spot causative agents.

5 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biochemistry, in particular to a method of increasing biomass or improvement of cell proliferation, leaf development and/or reproductive development of a plant, which includes increasing expression in the plant of nucleic acid, coding the ubiquitin-specific protease, as well as to the plant, a part of the plant and a seed of the plant, biomass of which is increased, or cell proliferation, leaf development and/or reproductive development of which are increased by the said method. A vector for application in the method of obtaining plants with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the plant and the part of the plant, transformed with the said vector, are described. Application of nucleic acid, coding the ubiquitin-specific protease is disclosed. Also disclosed is a method of obtaining a transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development in comparison with the control plants, obtained as a result of increased expression of nucleic acid, coding the ubiquitin-specific protease.

EFFECT: invention makes it possible to efficiently obtain the plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development.

17 cl, 11 dwg, 3 tbl, 28 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agricultural biotechnology. Method of producing dihaploid plants of barley from cultured microspores in vitro, comprising: growing donor plants at reduced air temperature of 15-20°C, light regime: 16 h day/8 h night, relative humidity of 60-70%, lighting intensity of 10000-15000 lux, with carrying out phytosanitary treatment, at that growing donor plants is carried out to the stage from opening of the sheath, when the inflorescence is in the flag leaf, - stress treatment of ears at 4°C, in tubes with a poor environment containing KCl - 1.5 g/l, MgSO4×7H2O - 0.25 g/l, CaCl2×2H2O - 0.1 g/l, mannitol - 60 g/l, potassium phosphate buffer - 1 ml/l, pH 7.0, for 7 days for shifting from gametophytic development path of microspore to sporophytic path - microspore isolation from spikelets in sterile conditions, - culturing the isolated microspores in a modified environment for embryogenesis induction, consisting of 6% maltose, 10 sets per 1.5 ml of the culture, and plant growth regulators in amount of 1 mg/l 2,4-D, 0.2 mg/l zeatin, and adding the aforementioned components is carried out before the addition of microspores, - regenerating plants from embryoids by culturing on solid nutrient Murashige and Skoog medium without the adding of plant growth regulators, - processing the barley haploid plants with antimitotic agent N-diacetyl-N-(β,γ-epoxypropyl) aminocolchicine for doubling chromosomes and obtaining dihaploid plants.

EFFECT: invention enables to obtain dihaploid homozygous lines of barley for breeding new varieties and hybrids with improved properties.

1 dwg, 2 tbl, 6 ex

FIELD: biotechnology.

SUBSTANCE: invention is a method of clonal micropropagation of apple tree rootstocks MM 106, CK 2, CK3, CK 4, CK 7, where in the steps of administering to the culture, the antibiotic griseofulvin 500 mg/l is added to the Murashige and Skoog medium as a sanifying agent.

EFFECT: invention enables to increase the yield of rehabilitated apple tree rootstocks obtained by meristem method in vitro by reducing the proportion of explants died from infection with fungal and morilioid infection.

1 tbl, 7 ex

FIELD: agriculture.

SUBSTANCE: invention is a method of reproduction of carnation in vitro, comprising separating explants, sterilisation, planting to Murashige and Skoog nutrient medium supplemented with sucrose 30 g/l and growth regulators 1 mg/l 6-benzylaminopurine and 0.2 mg/l naphthylacetic acid, micropropagation by removal of shoots from explants, their rooting in the Murashige and Skoog nutrient medium and further rooting in the ground, characterised in that in the micropropagation the explants are rooted on agar Murashige and Skoog nutrient medium supplemented with Ribav-Extra 0.01-0.1 mg/l, and rooted under the conditions ex vitro in the ground consisting of a mixture of humus - 50%, lowland peat with black mould humus - 30% and sand 20%.

EFFECT: invention enables to simplify the process of micropropagation.

1 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to agricultural biotechnology. The invention is a method of reproduction of tea tick trefoil, when the matured sterilized seeds of tea tick trefoil (Hedysarum theinum Krasnob) are planted in the Murashige-Skoog culture medium for insertion into tissue of the culture, in 20-30 days the developed shoots are transplanted to the MS medium of reproduction, then the formed conglomerates of microshoots are divided and transplanted to the media comprising 1.0 BAP + L-glutamine and adenine sulphate 100 mg/l. The plants-regenerants are reproduced, rooted and adapted to non-sterile conditions.

EFFECT: invention enables to improve the efficiency of the process, replicability, adaptation under conditions of hydroponics, and to obtain standard seedlings of tea tick trefoil.

4 dwg, 1 tbl

FIELD: biotechnologies.

SUBSTANCE: invention represents a method of clonal microreproduction of grapes in vitro with decontamination from mycoplasmal infection comprising the micro-grafting of lab plants, their planting in the solid Murashige and Skoog medium without growth stimulants and with decreased content of macronutrients. Meanwhile the nutrient medium is added by the antibiotic gentamycin in two stages: at the first subculturing gentamycin at the concentration 0.05-0.3 ml/l is added; at second stage- gentamycin at the concentration 0.01-0.04 ml/l is added simultaneously with adding of the preparation emistim deluted down to 10-6-10-12 ml/l.

EFFECT: offered method allows to improve efficiency of clonal microreproduction of grapes in the culture in vitro, to improve the survival ability and yield of plants cured from mycoplasma with the improved morphogenesis and quality characteristics.

7 tbl

FIELD: biotechnology.

SUBSTANCE: invention is a method of clonal micropropagation of grape in vitro, comprising micro-grafting of test tube plants, planting them on nutrient medium in the presence of the growth substances and cultivation, where planting the cultivation is carried out on a solid Murashige and Skoog nutrient medium containing the growth-regulating substance as the Melaphen preparation in the concentration of 10-7-10-11%.

EFFECT: method enables to increase the yield of the plants due to effective survival of micro-grafts, good regeneration, improvement of quality indicators of plants.

6 tbl

FIELD: biotechnologies.

SUBSTANCE: invention represents a method of reproduction of buckwheat in vitro, comprising a buckwheat reproduction from aseptic seed plantlets. The seeds are pre-sterilised with concentrated sulphuric acid and cultivated in Murashige and Skoog medium without phytohormones. They are subcultivated, using the shoot apex with an auxiliary bud with obtaining of microplants in the nutrient Murashige and Skoog medium with phytohormones containing from 9.2 up to 23.0 mg/l cuprous sulphate, which are separated into quicksets, cultivated, subcultivated and implanted in this medium.

EFFECT: offered method allows to improve efficiency of process of reproduction of buckwheat microplants in conditions in vitro due to its simplification, acceleration, cost saving and gain in yield of lab plants.

3 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture, in particular to selection. The invention is a method of increasing the coefficient of white cabbage reproduction under conditions in vitro, comprising growing the explants, cultivation of them in a nutrient medium of Murashige and Skoog, applying to it of growth regulators thidiazuron at a concentration of 1 mg/l in combination with the indolyl-3-acetic acid - 0.5 mg/l, using receptacles with the size of 0.2-0.3 mm, isolated from buds with the length of 0.5-0.7 mm, where the receptacle is used 1-2 days before flower blooming, and after growing them in nutrient media it is cultured till buds formation for 14-21 days.

EFFECT: method enables to obtain greater amount of regenerated plants with signs of CMS and to obtain genetically stable crop of selective samples.

3 tbl

FIELD: agriculture.

SUBSTANCE: method of regeneration of adventive microshoots Hyssopus officinalis L. under conditions in vitro comprises separating of leaf discs from microshoots after 4-5 passage, obtained in culture in vitro, their additional cultivation on a modified Murashige and Skoog growth medium with half concentration of macronutrients and supplemented with substance of cytokinin type of action, namely, with tidiazuron, and the cultivation is carried out at reduced intensity of illumination.

EFFECT: improvement of the method.

FIELD: agriculture.

SUBSTANCE: invention relates to agricultural biotechnology. The invention is a method of propagation of plant of white cinquefoil (Potentilla alba) by the method of culture in vitro, comprising separation of vegetative buds from rhizome, sterilisation, planting on nutrient media, micropropagation of shoots, rooting of shoots, adaptation of regenerated plants to non-sterile conditions.

EFFECT: use of the claimed method enables to obtain more than 150 thousand seedlings per year from one explant.

5 dwg, 1 tbl

FIELD: biotechnology.

SUBSTANCE: method involves preparing plants-donors of meristems for freezing, wherein plants-donors are toughened on solid medium supplemented with substances of cytokinic effect; separating apexes and treating with cryogenic protectors; cooling apexes in cryogenic ampoules and providing cryogenic keeping; performing post-cryogenic plant regeneration from cryogenically kept meristems. Method for cryogenic keeping of meristematic apexes isolated from in vitro toughened plants allows plants to be recovered from cryogenically kept meristems of red raspberry (Rubus idaeus L.), with callus forming stage being avoided.

EFFECT: increased efficiency and simplified method.

1 dwg, 2 tbl, 2 ex

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