Automated extraction of nucleic acids by size

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Invention is described, which includes method for selection of nucleic acids by size. Method involves stages of movement of nucleic acids from sample via channel by means of electrophoresis, automatic tracking of movement of reference fraction of nucleic acids via channel, assessment of estimated time of arrival of target fraction of nucleic acids in hole for extraction in channel, extraction of fluid medium containing target fraction from hole to extract at estimated time of arrival.

EFFECT: invention extends range of products for selection of nucleic molecules by size.

44 cl, 15 dwg

 



 

Same patents:

FIELD: measurement equipment.

SUBSTANCE: proposed invention relates to instruments of measurement of charged particles in samples for tests in biology, chemistry, industry or ecology, in particular, to instruments for measurement of ion concentration, for instance, lithium ion concentration in samples, such as blood samples. The technical result is achieved due to the fact that an instrument (1) for measurement of concentration of one type of charged particles in the sample (10), the first chain with a unit (54) of voltage control, which is connected to two first electrodes (30, 30'), located along the channel (12), containing a sample (10); the second chain with a unit (55) for detection of specific conductivity, which is connected to at least two second electrodes (5, 5'), placed in the channel (12), besides, the first chain and the second chain have electric isolation from each other; and the channel (12) contains fluid medium, containing gas, concentration of which inside the channel (12) is maintained at the level not exceeding the specified limit value. At the same time the first chain and the second chain have electric isolation for facilities of one or several transformers.

EFFECT: higher accuracy to measure concentration of charged particles.

10 cl, 8 dwg

FIELD: biotechnologies.

SUBSTANCE: differentiation of four Trichobilharzia species: T. szidati, T.regenti, T.franki and T.sp.var.narochanica is performed by amplification of sections of sequence of nuclear ribosomal DNA (rDNA) in specimens of reproductive helmints, their larval stage and/or on rDNA of fresh-water mollusks of Lymnaeidae family, which are infected with the above helmints by PCR and four oligonucleotide primers of the following composition: F: 5'-CTTTCCATCTATCACGATGCACT-3' R1: 5'-ATGATAATGTGCATAACACACC-3' R2: 5'-GCCGTTTATTTATATGTATGTG-3' R3: 5'-CAAGCCGTTTATTWATATATAACGG-3'. The obtained amplification products are visualised and differentiated and identified as per size (length); with that, one amplification fragment with the size of 255 base pairs is relevant for T.regenti, species, one fragment with the length of 316 base pairs is relevant for T.szidati species, two fragments with the size of 255 and 316 base pairs are relevant for T.sp.var.narochanica species, and amplification fragment with the size of 258 base pairs is detected only for T.franki species.

EFFECT: method allows simultaneous detection and species identification of fours types of ornithic schistosomes of Trichobilharzia species at different life stages.

2 dwg, 2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: diagnostic technique for urinary stone disease offers an integrated approach involving the determination of uric acid, creatinine, citrate, oxalate, cations and anions of inorganic salts, including urine sample preparation, quantitative analysis of these markers by capillary electrophoresis with UV detection at a certain polarity, detection wavelength and buffer electrolyte composition according to the analytical form and structure of the markers by preconditioning of a quartz capillary.

EFFECT: large number of the markers and their quantitative values allow a more accurate assessment of the deviations from the norm in the test object and improve the accuracy of the early diagnosis of urinary stone disease.

8 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: method involves adding C6-alkyloxybenzene (hexylresorcinol) into gel electrophoresis buffer. The invention increases the period of possible visual detection of separate DNA molecules during prolonged exposure thereof on a transilluminator at 254 nm, amplifies contrast properties of the background and preserves structural and functional characteristics of separate DNA molecules when detecting gel electrophoresis results by viewing in UV radiation.

EFFECT: improved protection.

1 dwg, 4 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: patient's blood is analysed for the erythrocyte membrane glycophorin concentration by gel electrophoresis assay and the malonic aldehyde concentration by spectrophotometric analysis. Then a discriminant equation D =-2.719 glycophorin +{+25.331 malonic aldehyde} is solved. If D is equal to or exceeds a boundary value +9.52, higher glycophorin activity in erythrocyte membranes is predicted in pregnant women.

EFFECT: higher prediction accuracy.

2 tbl, 3 ex

FIELD: agriculture.

SUBSTANCE: invention may be used for selecting immune and highly resistant to cocco mycosis sweet cherry and cherry varieties and root stocks for the above crops. During active leaves growing, they are subject to diagnostic analysing by producing UHF extract of phenol compounds. The produced extract is diluted with the distilled water at a ratio 1:4-1:5 and a sample is supplied to amperometric analyser. The type resistance to infection is judged based on the availability of detector signal at 0.4 V potential on glassy-carbon electrode.

EFFECT: reduced time for infection-resistant types selection and essential improvement of results reliability.

2 dwg, 4 ex

FIELD: chemistry.

SUBSTANCE: invention concerns separation of free gene-coded amino acid mix by method of capillary electrophoresis and can be applied in quality control for medicines and in defining amino acid composition of bioactive peptides. Sodium dihydrophosphate solution with methanol (pH 1.9-2.1) added is proposed as background electrolyte. Analysis is performed at +9-+11 kV voltage. The result is enhanced selectivity of free gene-coded amino acid separation leading to complete separation of 16 amino acid mix.

EFFECT: possibility of amino acid analysis of bioactive peptides.

1 cl, 1 ex, 2 tbl

FIELD: physics.

SUBSTANCE: chamber for simultaneous experiments set using by electrophoresis method contains number integrated electrophoretic cells each of which has a platform, electrodes, covers and slides, temperature-sensitive element and heaters. Platforms are provided with stepped holes for slides with covers frames on the surface. At that the platform has pockets that are electrode boxes in which electrodes are placed. Electrode boxes are covered with condensing lids. Stepped holes grooves contain attached frames with fixed semipermeable membranes. Besides, the chamber is supplied with holding jigs for fixation on microscope stage and allowing for motion of electrophoretic cells relative to microscope objective.

EFFECT: higher testing accuracy and improved performance.

12 cl, 7 dwg

FIELD: medicine.

SUBSTANCE: identification method applied for complicated object identification by means of its characteristic electrophoretic profile structure by biological active compound in modes capillary zone or micellar electrophoresis with UV-detection includes initial off-line sample component concentrating - within sample preparation (solid- or liquid-phase extraction), and then online concentrating (stacking) - directly in quartz capillary within elecrophoretic separation using complexing agents both for sample preparation, and for elecrophoretic separation. Object identification is based on comparison of characteristic profiles corresponding to certain reference object.

EFFECT: provided express; simplicity and informativity; improved efficiency and selectivity of object characteristic component separation and lowered detection limit.

2 ex, 4 dwg

FIELD: agriculture, horticulture.

SUBSTANCE: claimed method includes production of microwave-extract from cherry leaves; separation thereof by capillary electrophoresis method. Presence of gallic acid derivative peak with retention time of 11.0-12.3 min on electrophoresis pattern is indicated as form having resistance to Coccomyces blight of cherry.

EFFECT: accelerated method with increased reliability.

20 dwg, 3 ex

FIELD: chemistry.

SUBSTANCE: method includes treating a sample with a denaturing buffer solution, sorbing short RNA on a fibre glass sorbent in the presence of a chaotropic agent, followed by washing off non-bonded biopolymers and chemical reagents from the sorbent and eluting the end product. The biological fluid sample undergoes two-step denaturing, wherein at the first step two volumes of the denaturing buffer solution are added to the sample and at the second step two volumes of 96% ethanol and equal volume of chloroform are added to the obtained mixture, followed by stirring and separating the residue by centrifuging. Non-bonded biopolymers are washed off from the sorbent twice with the buffer solution and elution of the end product from the sorbent is carried out with the buffer solution.

EFFECT: simple method, short duration of the method and high output of short RNA.

3 cl, 3 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: claimed invention relates to the field of biotechnology and deals with a method of detecting a provirus of cattle leukosis. The characterised method includes the detection of LTR (Long Terminal Repeat) fragment - a sequence of the leukosis provirus. Determination of the size of an amplified fragment of a nucleotide sequence by the electrophoretic separation in an agarous gel is performed. As primers applied are oligonucleotides: BL1.F 5-GAGTTAGCGGCACCAGAAGC-3 and BL1.R 5-ATAGAGCTCGCGGTGGTCTC-3. The product of synthesis constitutes 175 pairs of the nucleotides. A genomic DNA is extracted by a sorbent method with the following elution in TE buffer, amplification is carried out in the mode: 95C for 3 minutes 1 time, 94C for 20 seconds - denaturation, 66C for 20 seconds - annealing of the primers, 74C for 25 seconds - elongation, 45 times, 74C for 3 minutes - chains completion. As a positive control of reaction proceeding applied is a preparation of a positive control of BLV antigen.

EFFECT: invention can be applied in the molecular-genetic diagnostics of animal diseases and scientific research in veterinary.

2 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to biochemistry. Described is method of detecting presence or absence of several serotypes of human papillomavirus (HPV) in biological sample by means of multi-channel analysis system, where said analysis system detects larger quantity of serotypes than the number of existing channels of detection. Method peculiarity consists in the fact that first and second sets of primers and probes, which are degenerate with respect to each other, are used; as well as the fact that each degenerate probe has signal grouping, each of which studies signal, detected on one and the same channel. Third set of primers and probes is not degenerate with respect to two other sets of primers and probes and is distinctive for third serotype, which does not belong to serotypes, to which mainly annealing of degenerate sets of primers and probes takes place, and where third set of primers and probes has signal grouping, which emits signal with wavelength, which is the same or differs from wavelength, emitted by signal grouping of degenerate probes. Respective primers and probes are presented.

EFFECT: invention makes it possible to detect larger quantity of HPV types than the number of existing channels of detection in analysis system.

11 cl, 3 dwg, 6 tbl

FIELD: medicine.

SUBSTANCE: inventions relate to the field of DNA-genealogy and deal with a method of determining haplogroups of human Y-chromosomes, a test-system and oligonucleotide primers. The characterised method is realised in two stages. At the first stage genetic typing is carried out by basic haplogroups R,N,J,I,Q,C,E,D,G,O of an Y-chromosome tree by multiplex PCR with the application of specific oligonucleotide primers of the first and second sets of the test-system. At the second stage, if mutation is detected, additional multiplex PCR for typing by subhaplogroups is carried out. If at the first stage mutation is not detected, multiplex PCR for typing by rare haplogroups A,C3,F,H,K,L,O3 and T is carried out. The area of annealing primers outside mutation zones within the limits of species specificity corresponds to sequences from the first set of the test-system. The area of annealing of the oligonucleotide primers, directly adjoining the mutation zone within the limits of species specificity corresponds to sequences from the second set of the test-system.

EFFECT: inventions can be applied for the determination of haplogroups of the human Y-chromosome.

3 cl, 2 dwg, 7 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: DNA is recovered from peripheral venous blood which is followed by a genetic typing of the APOE gene and detecting polymorphous alleles APOE*2, APOE*3, APOE*4; if the assays shows any genetic types containing alleles APOE*2, a high risk of endometrial cancer is predicted.

EFFECT: invention provides a highly specific criterion for predicting the risk of hyperproliferative diseases of the endometrium, including endometrioid adenocarcinoma in females with hyperplastic processes in the endometrium.

2 dwg, 10 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to a method for the prediction of developing occupational hyperkeratosis. Substance of the method consists in recovering DNA from peripheral venous blood lymphocytes, genotyping TP53 gene rs1625895 polymorphism by polymerase chain reaction with restriction enzyme digest analysis. Finding G/A genotype and A allele ensured predicting a risk of developing occupational hyperkeratosis.

EFFECT: using the invention enables predicting developing occupational hyperkeratosis after the body has been exposed to occupational hazards.

2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: presented group of inventions refers to medicine, molecular biology and biotechnology. A method for determining lung cancer cell sensitivity to cisplatin involving determining MLH1, ERCC1, DDB2, AKR1B1, FTL genes expression patterns vs. cisplatin IC50 for known cell lines; constructing cisplatin IC50 for these cell lines vs. derived gene expression patterns calibration straight, and hybridising on a microchip. What is also presented is a set of oligonucleotide probes presented by SEQ ID NO: 9-13.

EFFECT: presented group of inventions provides effective aids and methods for determining lung cancer cell sensitivity to cisplatin.

2 cl, 1 dwg, 2 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of biochemistry, in particular to method of identifying changes in gene expression, characteristic of ageing, in selected tissue. Selected tissue represents cardiac, muscular, cerebral or adipose tissue. Method includes selection of one or several genes, differentially expressed in tissue of old subjects in comparison with young subjects, with application of two criteria. Said criteria are: change of expression in at least 50% of lines, breeds or ethnic groups of tested species at preliminarily determined significance level p<0.10, as well as at least partial reversion of changes in gene expression by restriction in caloric content.

EFFECT: invention provides obtaining reliable tissue-specific biomarkers of ageing.

7 cl, 11 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: invention refers to molecular biology and can be used in diagnosing cardiomyopathies of various origin Presented is a set of synthetic oligonucleotides for identifying mutations of a coding part of desmin (DES) gene associated with cardiomyopathies. The mutations are identified by detecting a full sequence of the coding part of DES gene. The coding part of DES gene is amplified by means of 7 pairs of synthetic oligonucleotides at the same temperature and annealing time, and the prepared amplification products are sequenced by means of one pair of universal primers.

EFFECT: presented invention enables the sensitive and specific detection of DES gene mutations, with reducing the amplification reaction time, the number of manipulations, the time of agent application for the sequencing reaction, and reducing a probability of a reaction error.

3 cl, 1 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, more specifically to AXL signalling pathway inhibitors, and can be used in medicine. What is produced is a soluble AXL polypeptide version free from AXL transmembrane domain, which contains at least one amino acid modification in position No. n, wherein n is specified in 32, 72, 87, 92 or 127 or a combination thereof, wherein n+7 is described by numbering SEQ ID NO: 1 that are wild-type AXL sequences, wherein the above modification increases a AXL polypeptide binding affinity to protein 6 specifically inhibiting the growth (GAS6), which is twice as strong as the wild-type AXL polypeptide affinity. The polypeptide can be fused with Fc fragment and used in a method of treating, reducing or preventing tumour dissemination and invasion in a mammalian patient.

EFFECT: invention enables inhibiting the AXL/GAS6 signalling pathways effectively.

8 cl, 15 dwg, 6 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: method includes treating a sample with a denaturing buffer solution, sorbing short RNA on a fibre glass sorbent in the presence of a chaotropic agent, followed by washing off non-bonded biopolymers and chemical reagents from the sorbent and eluting the end product. The biological fluid sample undergoes two-step denaturing, wherein at the first step two volumes of the denaturing buffer solution are added to the sample and at the second step two volumes of 96% ethanol and equal volume of chloroform are added to the obtained mixture, followed by stirring and separating the residue by centrifuging. Non-bonded biopolymers are washed off from the sorbent twice with the buffer solution and elution of the end product from the sorbent is carried out with the buffer solution.

EFFECT: simple method, short duration of the method and high output of short RNA.

3 cl, 3 tbl, 4 ex

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