Microorganisms producing o-acetyl homoserine and method for o-acetyl homoserine production using this microorganism

FIELD: biotechnology.

SUBSTANCE: this microorganism is characterized by weakened or inactivated endogenous citrate synthase activity. As a result of this modification, the is microorganism capable of producing O-acetyl homoserine with high yield. The invention also relates to a method for O-acetyl homoserine production. This method comprises culturing of the said microorganism and separation of O-acetyl homoserine produced by culturing of this microorganism.

EFFECT: invention allows to obtain O-acetyl homoserine with high yield.

7 cl, 2 dwg, 10 tbl, 4 ex

 



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30°C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: biotechnology.

SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.

EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.

4 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: nutrient medium comprises sodium chloride, potassium chloride, magnesium sulphate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, sodium bicarbonate, L-arginine, L-glutamine, L-tyrosine, L-tryptophan, calcium pantothenate, pyridoxal HCl, thiamine, meso-inositol, nicotinamide, riboflavin, folic acid, glucose, enzymatic hydrolyzate of muscle proteins, lactalbumin enzymatic hydrolyzate, sodium salt of benzylpenicillin with activity of 0.09×105-1.1×105, kanamycin sulphate with activity of 0.09×105-1.1×105, nystatin with activity of 6×104 to 6.5×104 IU/l, choline chloride, succinic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to improve the quality of the product obtained.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.

EFFECT: invention makes it possible to increase the output of citric acid.

2 tbl, 6 ex

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25°C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70°C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns the strain Escherichia coli BL21(DE3)Gold/pETmin-CypA producing human recombinant cyclophilin A. The characterised strain is produced by cell transformation of the strain BL21(DE3)Gold by pETmin-CypA plasmid. The plasmid has a size of 5865 base pairs and contains XhoI-NdeI fragment of pET-22b(+) vector carrying ampR ampicillin resistance gene, a promoter and RNA-polymerase T7 phage terminator and a polylinker. A fragment produced by PCR with using the oligonucleotide primers 5'-TTATACATATGGTCAACCCGACCGTGTTCTTC and 5'-TTTCTCGAGTTATTCGAGTTGTCCACAGTCAGC of pCyPAwt/pGEX-2TK plasmid with the closed fragment of hrCpA (human recombinant cyclophilin A) gene having a size of 498 base pairs is cloned at XhoI-NdeI sites.

EFFECT: presented strain is high-producing; it requires no complicated clean-up system and can be used in cancer treatment.

2 dwg

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology, particularly to mutant O-phosphoserine sulfhydrylase (OPSS) from Mycobacterium smegmatis with an amino acid sequence corresponding to SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. The inventions also relate to a nucleic acid molecule encoding the mutant OPSS, an expression vector carrying the nucleic acid molecule, and a transformant transformed by the expression vector. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulphide in the presence of the mutant OPSS.

EFFECT: muntant OPSS has improved enzymatic activity and can be used for environmentally friendly production of L-cysteine in conditions through a simple enzymatic conversion reaction; the group of inventions provides high output of L-cysteine.

16 cl, 7 dwg, 20 tbl, 19 ex

FIELD: biotechnology.

SUBSTANCE: method of production of selenocystine consists in that 1 molar solution of sodium diselenide is prepared by adding 4.5 g of elemental selenium to 10 ml of 1 molar solution of sodium hydroxide (NaOH) in a three-necked flask. Then, 15 ml of distilled water is added and the solution is stirred using a magnetic stirrer. The separately prepared solution of natrium borane (NaBH4) by dissolving it in 25 ml of distilled water is added as drops to the three-necked flask with a suspension of elemental selenium to total decolorization of the solution, and another 4.5 g of elemental selenium are additionally added. 5 g β-chloro-l-alanine are dissolved in distilled water, and the solution is adjusted to pH 9 by adding 1M sodium hydroxide solution. Within 30 minutes the solution of β-chloro-l-alanine is added to the solution of sodium diselenide, located in the three-necked flask and stirred for 12-16 hours at a temperature of +37°C. Then, muriatic acid is added to the resulting solution dropwise to pH 2 and the air purging is carried out for 2-3 hours. The solution is filtered and adjusted with 10 M sodium hydroxide solution to pH 6-6.5. The solution is cooled to +5°C.

EFFECT: increase in the yield ratio and improved purity.

1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to biochemistry and represents a method for producing cysteine involving culturing a recombinant microorganism with the low activity of endogenous phosphoserin phosphatase (SerB) for producing O-phosphoserin (OPS) and contacting the prepared culture containing OPS, or OPS recovered from the culture with sulphide in the presence of O-phosphoserin sulphhydrilase (OPSS) or a microorganism expressing OPSS for preparing cysteine with the recombinant microorganism representing a recombinant bacteria. The invention refers to preparing a cysteine derivative which provides preparing cysteine as presented above to be transformed into the cysteine derivative.

EFFECT: invention enables producing cysteine and its derivative with high efficacy.

31 cl, 6 dwg, 35 tbl, 49 ex

FIELD: chemistry.

SUBSTANCE: disclosed is bacteria of the Enterobacteriaceae family - a producer of L-cysteine, which is modified such that expression of the following is intensified therein: one or more genes of the cysDNC cluster participating in sulphate activation; or one or more genes of the cysDNC cluster participating in activation of sulphate and a cysQ gene participating in decomposition of adenosine-3'-phosphate-5'-phosphosulphate (PAPS). Disclosed are methods of producing L-cysteine, L-cystine, S-sulphocysteine and L-cysteine thiazolidine derivative, involving: growing said bacteria in a culture medium which contains sulphate; and respectively separating L-cysteine, L-cystine, S-sulphocysteine or L-cysteine thiazolidine derivative from the culture fluid.

EFFECT: invention increases output of said products.

20 cl, 2 tbl, 5 dwg, 6 ex

FIELD: chemistry.

SUBSTANCE: described is L-cysteine producing bacteria, which relates to the Pantoea or Escherichia genus of the Enterobacteriaceae family, which is modified such that it has stronger expression of one or more genes selected from a group consisting of glutaredoxin coding genes grxC and grxB, and a glutathione reductase coding gene (gorA). Disclosed is a method of producing L-cysteine, involving growing said bacteria in a culture medium containing thiosulphate, and separating L-cysteine from the culture fluid.

EFFECT: invention increases output of L-cysteine.

9 cl, 4 tbl, 4 dwg, 5 ex

FIELD: food industry.

SUBSTANCE: starch-containing raw materials (cereals) c are milled to produce a milled product including solid starch-free components in an amount of no less than 20 wt % One prepares a water-based suspension of the milling product to ultimately obtain flour pulp with dry weight content equal to at least 45 wt %. The pulp is heated in a jet boiler by way of water steam delivery to a temperature exceeding that of starch gelatination. One performs flour pulp hydrolysis by way of dilution and subsequent saccharification in the presence of α-amylase enzyme in a quantity of 0.002 - 3.0 wt % of the total quantity of the starch-containing raw material used to produce water medium M. Water medium M is added to the fermentative medium wherewith one cultivates a microorganism capable of the organic compound hyperexpression for fermentative broth production.

EFFECT: method enables production of an organic compound represented by a solid or partly solid non-volatile product of microbial metabolism such as monocarboxylic, dicarboxylic and triocarboxylic acids acid with 3 - 10 carbon atoms carrying, in case of necessity, hydroxyl groups, proteogenic and non-proteogenic amino acids, vitamins and biopolymers.

13 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: disclosed method of producing methionine involves: a) culturing a recombinant microorganism C. Glutamicum, which is deposited at the German Collection Microorganism And Cellular Structures DSMZ under No.DSM 17322, in suitable conditions, and b) extracting methionine.

EFFECT: obtaining methionine.

2 cl, 16 dwg, 19 tbl, 13 ex

FIELD: medicine.

SUBSTANCE: method includes cultivation of methionine-producing microorganism, belonging to genus Corynebacterium glutamicum or E. coli, in presence of dimethyl disulfide or slowly releasing system of dimethyl disulfide (DMDS) delivery, ensuring methionine production.

EFFECT: invention makes it possible to obtain methionine with high degree of efficiency.

23 cl, 11 dwg, 14 tbl, 12 ex

FIELD: medicine.

SUBSTANCE: method includes heating of fermentation broth, which contains methionine and biomass and is produced in process of fermentation of microorganism-methionine producer, with further separation of biomass from fermentation broth to produce liquid phase enriched with methionine, besides separated biomass is washed with water, and produced solution is combined with mentioned liquid phase. Produced methionine is crystallised out.

EFFECT: increased efficiency of methionine extraction.

14 cl, 4 dwg, 5 ex

FIELD: biotechnology, genetic engineering, amino acids.

SUBSTANCE: invention relates to a method for preparing L-methionine by culturing the microorganism strain transformed with plasmid comprising gene yjeH or gene with homology with its by above 30% and used as a producer of L-methionine. Prepared L-methionine is isolated from medium. The claimed invention provides preparing L-methionine with high degree of effectiveness.

EFFECT: improved preparing method.

11 cl, 1 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of biotechnology. Claimed is method of obtaining 4-hydroxy-L-leucine or its salt by method of enzymatic conversion of L-leucine or its salt in presence of bacterial deoxygenase, selected from group, which consists of dioxygenases with amino acid sequence SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 54, SEQ ID NO: 62 or their versions. Described is method of obtaining 4-hydroxy-L-leucine or its salt in L-leucine-containing medium in presence of first bacterium, transformed with DNA molecule, encoding dioxygenase, which consists of dioxygenases with amino acid sequence SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 54, SEQ ID NO: 62 or their versions. Described is bacterium - producent of 4-hydroxy-L-leucine or its salt, transformed with DNA molecule, encoding bacterial dioxygenase, selected from group, consisting of dioxygenases with amino acid sequence SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 54, SEQ ID NO: 62 or their versions in medium, containing L-leucine or its salt.

EFFECT: invention makes it possible to obtain 4-hydroxy-L-leucine or its salt with high degree of efficiency.

9 cl, 24 dwg, 5 tbl, 11 ex

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