Composition for producing hydrogel based on polyvinyl alcohol for immobilization of microorganisms
SUBSTANCE: composition for producing hydrogel based on polyvinyl alcohol for the immobilization of microorganisms is described, consisting of a polyvinyl alcohol and a catalyst of cerium-ammonium nitrate crosslinking, characterized in that the initial components are taken in the molar ratio of EVOH: (NH4)2Ce(NO3)6 (160-110):(1-1.3).
EFFECT: ensuring formation of water-insoluble hydrogel for immobilization of microorganisms, improved accuracy and sensitivity of the assay using the biosensor.
18 cl, 1 dwg,1 tbl, 1 ex
SUBSTANCE: group of inventions relates to medicine, namely to therapeutic treatment of gout and prevention of accompanying side effects and complications in patients. method of prevention and prediction of risk of infusion responses in case of intravenous introduction of pegylated uricase to patients with gout consists in determination of uric acid content in patient's blood serum with the following indication to cessation of therapy at the level of approximately 4 mg/dl. Claimed method also makes it possible to identify risk group of patients, who receive pegylated uricase, with reduction of treatment efficiency due to formation of antibodies and higher risk of infusion response development.
EFFECT: group of inventions provides timely cessation of treatment to prevent infusion responses in patients with gout in treatment with pegylated uricase.
37 cl, 2 tbl, 6 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology. What is presented is an enzyme biocatalyst in the form of nanoscale particles for in vivo detoxification of organophosphorous compounds. The biocatalyst represents non-covalent polyelectrolyte complexes. The given complexes consist of a polyhistidine-containing polypeptide with the properties of organophosphosphorous hydrolase and polyethylene glycol-polyglutamic acid block copolymer in a charge relation of enzyme:block copolymer falling within the range of 2:1 to 1:5.
EFFECT: invention provides significant simplification of the biocatalyst technology, an increase of its catalytic efficiency, a dose decline, an immunotoxic suppression, as well as higher activity in hydrolysis reactions of pesticides and toxic materials.
4 cl, 6 ex
SUBSTANCE: invention describes a method for preparing a biospecific hydrogel sorbent for proteinase recovery by the radical polymerization of an aqueous solution containing ovomucoid 0.1-0.9 wt % of duck egg protein acylated by acryloyl chloride, acrylamide 7.0-12.0 wt % and N,N'-methylenebisacrylamide 0.5-1.1 wt % at room temperature under the action of a redox polymerisation catalyst; the prepared hydrogel is ground and washed in a bicarbonate buffer to remove the reacted compounds completely with the polymerisation conducted in mercaptoacetic acid 0.01-0.12 wt %.
EFFECT: more effective use of ovomucoid.
1 tbl, 14 ex
SUBSTANCE: ferment lovastatin esterase is proposed, which is immobilised on a water-insoluble hard carrier activated with a bifunctional agent. At the same time the hard carrier represents a modified di-(C1-6alkyl)amino-C1-6alkylcellulose, in another version the hard carrier represents a silica gel modified with amino-C1-6alkyl-tri(C1-6alkoxy)silane, and the bifunctional agent that activates the hard carrier represents O-sulfonate of cyanuric acid or acid halide of cyanuric acid. In the third version the hard carrier represents agarose, and the bifunctional agent is a compound that corresponds to the formula , as defined in the formula. Methods (versions) are proposed to immobilise the ferment lovastatin esterase on specified water-insoluble hard carriers. According to the methods, in process of mechanical mixing the bifunctional activating agent is brought in contact with the hard carrier in the dissolvent. The activated hard carrier is separated by filtration, then dried and suspended in a water mixture, containing the ferment lovastatin esterase, with performance of ferment immobilisation. The suspended substance is separated by filtration, washed with the buffer solution and dried. Also the method is proposed to treat simvastatin, including treatment of the simvastatin salt solution, containing the remaining amount of the lovastatin salt, immobilised witht the ferment lovastatin esterase, and a biocatalysed flow reactor is proposed with a layer for realisation of this method. The reactor comprises a reactor body (1) with the inner space (2), connected with a liquid inlet (3) and connected with a liquid outlet (4), in the inner space there is a perforated plate supporting the layer (5), containing the ferment lovastatin esterase, immobilised on the water-insoluble hard carrier.
EFFECT: immobilised lovastatin esterase according to the invention demonstrates at least 5 times higher hydrolytic activity in respect to lovastatin and lovastatin salts in presence of simvastatin and simvastatin salts than in respect to simvastatin and simvastatin salts.
18 cl, 2 dwg, 8 tbl, 20 ex
SUBSTANCE: what is presented is a composition for producing a polymer film for microorganism immobilisation in biosensor analysers. The composition consists of polyvinyl alcohol and N-vinylpyrrolidone copolymer. The initial ingredients are taken in the molar ratio of PVA-VP 239:(9.0-56.2).
EFFECT: composition provides high biosensor sensitivity in stable operation up to 40 days.
1 dwg, 1 tbl, 1 ex
SUBSTANCE: what is used as a hepatoprotector is hyaluronidase immobilised on a low-molecular water-soluble polymer with using an electron-beam synthesis nanotechnology. Using this agent enables both stimulation of regional precursor cells and considerable extension of an elementary cell pool of depot tissue (bone marrow), their mobilisation in peripheral blood and targeted homing into an affected organ.
EFFECT: ensured manifested hepatoprotective effects with underlying lower risk of complications.
3 tbl, 1 ex
SUBSTANCE: in order to produce a biospecific polymer sorbent for extracting proteinase, an aqueous solution undergoes radical polymerisation, where said solution contains 0.1-1.5 wt % ovomucoid from duck egg albumen, acylated with acyl chloride of acrylic or methacrylic acid, 7.0-20.0 wt % hydrophilic monomer consisting of acrylamide, methacrylamide or N-vinylpyrrolidon, and 0.7-15.0 bifunctional cross-linking agent. The hydrophilic monomer is further mixed with N-ethylbromide dimethylaminoethyl methacrylate, taken in amount of 45-55 wt % with respect to weight of the hydrophilic monomer.
EFFECT: addition of N-ethylbromide dimethylaminoethyl methacrylate to the hydrophilic monomer increases efficiency of sorption of proteinase from biological fluids.
1 tbl, 2 ex
SUBSTANCE: offered is subtilisin immobilised on a water-soluble pharmacologically acceptable polymer and produced of Bacillus licheniformis bacteria by deep fermentation. The immobilisation is enabled by exposing an aqueous solution containing subtilisin and the pharmacologically acceptable polymer to ionising radiation. The polymer is specified in a group: polyethylene oxide, polyvinylpyrrolidone, polyacrylimide, Pluronic, dextrane. Before the exposure, the reaction mixture is frozen to (-20) - (-140)°C.
EFFECT: immobilised subtilisin exhibits anticoagulant and thrombolytic properties and has a less evident toxic effect.
4 cl, 9 tbl, 3 ex
SUBSTANCE: biologically active substance (BAS) is dissolved in a water solution of polymer concentrated more than 10 % to concentration of a saturated solution. The prepared reaction mixture is exposed to ionising radiation. The second version of the method consists in the fact that the BAS is dissolved in a water solution of polymer, then the mixture is frozen to temperature (-20°)-(-140°) C and exposed to ionising radiation. A BAS-carrier conjugate prepared by the offered methods is characterised by the fact that the BAS is specified in a group: proinsulin, tyrozyl-2-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate, alkalase, hyaluronidase, somatotropin, and the carrier is specified in a group: polyethylene oxide, polyvinyl pyrrolidone, dextrane, pluronic, acrylamide. A BAS molecule is covalent bounded with at least one polymer molecule.
EFFECT: invention enables immobilisation on the polymer carrier of those water-soluble BAS which are unstable, destroyed when immobilised by the other methods completely or almost completely, and increase in a portion of the preserved BAS when the immobilised BAV is destroyed incompletely.
23 cl, 34 dwg, 3 tbl, 20 ex
FIELD: chemistry of polymers, biochemistry.
SUBSTANCE: invention relates to a rapid method for preparing photoreactive polymers and immobilization of biomolecules on indicated polymers. Invention describes a method for preparing photoreactive polymers followed by immobilization of molecules on them that involves the following steps: (a) effect of microwave radiation on mixture of photolinker and solvent; (b) washing out polymer with a corresponding solvent followed by drying at the environment temperature to obtain photoreactive polymer; (c) addition of biomolecule dissolved in corresponding buffer on photoreactive polymer; (d) treatment of mixture by the photoenergy source for period from 2 min to 2 h for immobilization of molecule on indicated polymer, and (e) washing out indicated polymer having immobilized molecule in corresponding buffer and the following drying indicated polymer at the environment temperature to obtain photoreactive polymer with molecules immobilized on its.
EFFECT: improved preparing method.
22 cl, 2 tbl, 18 ex
SUBSTANCE: invention relates to water-soluble films. Water-soluble film has suitable thickness and contains, at least, 50 wt % of water-soluble resin, based on polyvinyl alcohol (PVA), resin, having average viscosity in the range from approximately 13.5 sP to approximately 20 sP and degree of hydrolysis in the range from approximately 84% to approximately 92%, film in addition has not more than 30 wt % of PVA-polymer, which has average viscosity lower than approximately 11 sP, in addition, film is characterised by solubility index in the range from approximately 620 to approximately 92, when film is approximately 76 micron thick, and stress index in the range from approximately 145 to approximately 626, when film is approximately 76 micron thick, and where film is thermally mouldable. Sack, containing water-soluble film, is also claimed.
EFFECT: water-soluble films and sacks, containing water-soluble films, have good cold water solubility, moisture resistance in treatment with wet hands and good mechanical properties.
9 cl, 15 tbl, 3 dwg
SUBSTANCE: invention relates to improved products, used in agriculture, including solid materials. A polymer-containing composition, intended for the application on solid products, applied in agriculture, includes water, a copolymer, which contains individual quantities of maleic and itaconic residues, and from approximately 5 to 60 wt % of a drying accelerator, representing an organic compound, selected from the group, consisting of organic alcohols and ketones. Also claimed is a version of the polymer-containing composition.
EFFECT: increase of the mass yield.
19 cl, 2 tbl, 2 ex
SUBSTANCE: described is a proton-conducting composite polymer material which includes a linear polymer matrix which is a 2-9% aqueous solution of polyvinyl alcohol, containing silver nanoparticles with size of 20-100 nm in concentration of 40-100 mg/l and a proton-conducting solid electrolyte dispersed therein in the form of a phosphoric-tungstic acid and a plasticiser in the form of glycerine, with the following ratio of components, wt %: aqueous solution of polyvinyl alcohol 38-69, phosphoric-tungstic acid 19-50, glycerine - the balance.
EFFECT: proton-conducting composite polymer material having high ion conductivity and the lowest possible electronic component of conductivity, which improves power characteristics of supercapacitors or other solid-state electronic devices, and longer charge storage duration thereof.
2 tbl, 13 ex
SUBSTANCE: composition for producing hydrophobic fire- and water-resistant films contains aqueous solution of polyvinyl alcohol, formaldehyde and an acid curing catalyst - phosphoric acid, a dispersion of solid amino plastic particles based on products of condensation of formaldehyde with urea in form of fine particles of a urea-formaldehyde resin, with overall molar ratio of the mixture of amines to formaldehyde of 1:0.8-1.3, diethanolamine, a water repellent in form of a 50% aqueous dispersion. In a second version, the composition contains a mixture of carbamide with 10-30% melamine besides formaldehyde.
EFFECT: obtaining fire-resistant films endowed with water-repellent properties and water-resistance.
5 cl, 3 tbl
SUBSTANCE: composite material can be used to produce sheet decorating and heat-insulating materials in residential, agricultural and industrial construction, as well as for producing moulded packaging elements and containers susceptible to biodegradation, i.e., having biodegradable properties. The polymer composite material consists of, wt %: fibre filler - waste cardboard and/or paper 11.0-12.0, cationic-anionic polyacrylamide resin Ultrarez DS-150 56.0-57.0, polyvinyl alcohol in form of a 15 wt % aqueous solution 27.0-28.0, sodium tetraborate 6.0-3.0. Described is a method of producing a composite material which involves separating fibres of the fibre filler on a rotary disperser with rotor speed of 2500-3000 rpm, wherein the polyvinyl alcohol is added at the mixing step in form of a 15 wt % aqueous solution together with the cationic-anionic polyacrylamide resin Ultrarez DS-150 and sodium tetraborate, pressing and drying.
EFFECT: improved physical and mechanical properties of the polymer composite material while simplifying the production technique.
2 cl, 3 ex
FIELD: process engineering.
SUBSTANCE: invention relates to production of porous polyvinylformal-based filters for removal water, impurities and biological contaminants from fluids and gases. Proposed method consists in chemical modification of the following composition containing in wt %: polyvinyl alcohol - 50-20, pore-forming agent - 2-30, cross-linking agent - 2-15, catalyst - 3-15, water making the rest. This is effected by dissolving water-soluble preset-amount polyvinyl alcohol inorganic slat in aqueous solution at heating it to 75-95°C and continuous mixing. Plus adding preset amount of cross-linking agent thereto and mixing for 10-30 minutes. Plus adding preset amount of catalyst at 30-40°C to convert soluble salt into insoluble one and mixing for 10-20 minutes. Plus pouring produced reaction mix into moulds to be held in oven at 55-80°C for 5-20 hours. Now, produced element is extracted from the mould and cleaned of catalyst and pore-forming agent. Finally, it is dried, first, at 15-25°C for 24-48 h and, then, at 30-50°C to constant current.
EFFECT: efficient filter.
7 cl, 1 tbl, 3 ex
SUBSTANCE: method involves mixing equally concentrated, highly saponified aqueous solutions of polyvinyl alcohol and partially saponified polyvinyl alcohol and filler, where the filler is nano-bodies selected from fullerenes and nanotubes, taken in amount of 0.02-1.0 wt % with respect to the polymer.
EFFECT: combining two types of polyvinyl alcohol to obtain mixed compositions.
SUBSTANCE: invention relates to an oxygen impermeable polyolefin composition for use in making food packaging. The composition contains polyolefin, whose composition includes a copolymer of ethylene with a vinyl alcohol containing 27-44 mol % ethylene links, an active oxygen absorbent, a miscibility enhancing additive, as well as clay or a nucleating agent or a polyamide, optionally including said clay or nucleating agent. The clay has characteristic ratio of at least 10.
EFFECT: combining components in a defined ratio enables to obtain compositions with unexpectedly good properties as an oxygen barrier.
13 cl, 2 dwg, 3 tbl, 10 ex
SUBSTANCE: described is an aqueous solution of an optical brightener, containing (a) 10-50 wt % optical brightener of formula (I): where: M is hydrogen, an alkali metal atom, ammonia or an amine cation; R1 is hydrogen, C1-C4-alkyl or C2-C4-hydroxyalkyl; R2 - C1-C4-alkyl which can be substituted with -CN- or -CONH2-group or C2-C4-hydroxyalkyl; or R1 and R2 together with a nitrogen atom close a morpholine ring; (b) 0.5-9 wt % polyvinyl alcohol, having degree of hydrolysis 71-85.2% and Brookfield viscosity 3-5.4 mPa·s; and (c) water. The invention also describes use of said solution in a coating composition for paper and a method of obtaining paper coated with said coating composition.
EFFECT: disclosed aqueous solutions of optical brighteners have low viscosity, can be used directly by paper manufacturers and can be fed by a pump directly into the coating composition to provide a coating on paper with high whiteness.
10 cl, 2 tbl, 5 ex
FIELD: process engineering.
SUBSTANCE: invention relates to filters intended for separation of inhomogeneous systems, in particular, to fabrication of filtration PVFM-based materials to be used in cleaning fluids and gases of water, mechanical impurities and biological contaminants. Composition for filter fabrication includes the following components, in wt %: polyvinyl alcohol - 5-20, porophore - 2-10, paraformaldehyde cross-linking agent - 2-15, catalyst - 1-15, water making the rest. Porophore mat represent a mix of starches with average grain size, or mix of starch with dextrin. Preset amount of polyvinyl alcohol is diluted in water. It is heated to 75-95°C with continuous mixing. Porophore suspension in cold water is added to obtained solution and kept at 55-80°C for 15-30 min with continuous mixing. Preset amount of cross-linking agent is added thereto and mixed for 10-30 min. Preset amount of catalyst is added thereto at 30-40°C and mixed for 10-20 min. Obtained reaction mix is poured in the mould, kept in temperature controlled cabint at 55-80°C for 5-20 h. Produced element is withdrawn from said mould, washed of catalyst and porophore. Drying is carried out at 15-25°C and, then, at 30-50°C to constant weight.
EFFECT: simplified process, possibility to vary filter porosity, reduced contamination.
4 cl, 4 tbl, 16 ex
FIELD: production of high-molecular compounds.
SUBSTANCE: the invention is pertaining to the field of production of high-molecular compounds, in particular, to polymeric composition used for production of cryogels on the basis of polyvinyl alcohol. The offered polymeric composition having the interrelated macropores of cryogel of polyvinyl alcohol contains 3-25 mass % of polyvinyl alcohol, 0.001-1 mass % of ionogenic-cationic anionic either amphoteric or nonionogenic surface active agent and water (up to 100 mass %). In the process of the cryogenic treatment of the indicated polymeric compositions produce a macroporous cryogel with the size of interrelated macropores from 2 up to 10 microns, The macroporous cryogel may be efficiently used in the capacity of a biomedical application material.
EFFECT: the invention allows to obtain a macroporous cryogel, which may be efficiently used as a biomedical application material.
9 ex, 1 tbl