Yeast transformant schizosaccharomyces pombe, producing lactic acid (versions) method for production thereof (versions), method for lactic acid microbiological synthesis using this transformant

FIELD: biotechnology.

SUBSTANCE: transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed. Also a transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed, wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus,wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. Also, versions of the preparation method for these transformants and the method for microbial synthesis of lactic acid are proposed.

EFFECT: efficient production of lactic acid at low concentrations of by-product.

13 cl, 10 dwg, 8 ex

 



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: biotechnology.

SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.

EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.

4 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: nutrient medium comprises sodium chloride, potassium chloride, magnesium sulphate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, sodium bicarbonate, L-arginine, L-glutamine, L-tyrosine, L-tryptophan, calcium pantothenate, pyridoxal HCl, thiamine, meso-inositol, nicotinamide, riboflavin, folic acid, glucose, enzymatic hydrolyzate of muscle proteins, lactalbumin enzymatic hydrolyzate, sodium salt of benzylpenicillin with activity of 0.09105-1.1105, kanamycin sulphate with activity of 0.09105-1.1105, nystatin with activity of 6104 to 6.5104 IU/l, choline chloride, succinic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to improve the quality of the product obtained.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.

EFFECT: invention makes it possible to increase the output of citric acid.

2 tbl, 6 ex

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns the strain Escherichia coli BL21(DE3)Gold/pETmin-CypA producing human recombinant cyclophilin A. The characterised strain is produced by cell transformation of the strain BL21(DE3)Gold by pETmin-CypA plasmid. The plasmid has a size of 5865 base pairs and contains XhoI-NdeI fragment of pET-22b(+) vector carrying ampR ampicillin resistance gene, a promoter and RNA-polymerase T7 phage terminator and a polylinker. A fragment produced by PCR with using the oligonucleotide primers 5'-TTATACATATGGTCAACCCGACCGTGTTCTTC and 5'-TTTCTCGAGTTATTCGAGTTGTCCACAGTCAGC of pCyPAwt/pGEX-2TK plasmid with the closed fragment of hrCpA (human recombinant cyclophilin A) gene having a size of 498 base pairs is cloned at XhoI-NdeI sites.

EFFECT: presented strain is high-producing; it requires no complicated clean-up system and can be used in cancer treatment.

2 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. A disclosed polypeptide having the amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO: 1 or 2, revealed in description, which polypeptide has a capable of expressing the polynucleotide activity. Also a polynucleotide encoding D-lactate dehydrogenase originated from DNA construct in which the polynucleotide and a promoter capable of expressing the polynucleotide are linked is introduced. Also described a transformant for production of lactic acid, or transformed yeast, in which the polynucleotide or the DNA construct is introduced. A method of producing D-lactic acid, which comprises the step of culturing the said transformant.

EFFECT: transformant capable of highly producing D-lactic acid compared to the D-lactic acid produced with host cell.

15 cl, 2 dwg, 4 tbl, 13 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Claimed is recombinant strain of yeasts. Schizosaccharomyces pombe ARCIM Y-4041, producent of lactic acid. Strain is obtained by transformation of strain Schizosaccharomyces pombe ARCIM Y-3106 with integrative plasmid pcDNA-Km-leu1-pla, constructed on the base of vector pUC19 and containing gene of lactatedehydrogenase ldh1 of lactic acid bacteria Lactobacillus plantarum.

EFFECT: claimed strain in cultivation on YPD medium is capable of producing lactic acid in amount 50 g/l of culture liquid.

2 dwg, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to bioengineering of food products and can be used in processing fermentation solutions to obtain lactic acid. The method of extracting lactic acid from fermentation solutions involves extracting lactic acid with a salt of a quaternary ammonium base in a diluenet and re-extracting the acid, wherein extraction is carried out with a salt of a quaternary ammonium base in sulphate form [(R4N)2SO4], where R is an alkyl or aryl radical, in the presence of p-tertiary alkylphenols in molar ratio of (R4N)2SO4 to p-tertiary alkylphenols of 1:2, at solution pH 5.0-7.0; the acid is re-extracted with sodium hydroxide solutions and the extractant is regenerated via treatment with a stoichiometric amount of sulphuric acid.

EFFECT: high degree of extraction of lactic acid from fermentation solutions and preventing the chloride ion from falling into the aqueous phase after extraction.

4 cl, 5 tbl, 6 ex

FIELD: chemistry; food products.

SUBSTANCE: method of obtaining lactate-containing solutions involves cultivation Rhizopus oryzae mycelium fungus cells in medium with glucose-containing substrate, the cells immobilised in polyvinyl alcohol cryogel. Lactate ion sorption is performed over strong-base gel anionite, followed by elution of absorbed lactate ions from anionite. Obtained eluate is decoloured by passing through activated carbon layer at room temperature. Further concentration is performed by vacuum condensation at 30-40C. To obtain lactic acid solution, anionite in Cl--form is used, and elution is performed by hydrochloric acid solution with 0.15-0.5 M concentration. To obtain lactate solution, anionite in OH--form is used, and eluation is performed by 0.5-1.0 M NaOH or KOH alkali solution, or by KCl or NaCI salt solution, with concentration gradient from 0 to 3M or at constant concentration selected out of the same range, or by equimolar mix of indicated alkali and salt solutions.

EFFECT: obtaining target product with 940 g/l concentration and 98,2% purity.

3 cl, 6 ex

FIELD: biotechnology, in particular method for lactic acid production based on microbiological synthesis.

SUBSTANCE: lactic acid id produced by fermentation of sugar-containing medium with lactic acid bacteria of Lactobacillus delbrueckii strain at age of 48-72 h in presence of 12-15 g/l malt sprout, treated with water acidified with lactic acid to pH 4.0-5.0 and enzyme preparation xyloglucanofoetidinenin amount corresponding to 200-600 U/l of xylanase activity. Said medium contains chalk in amount of 60 % based on total amount calculated for lactic acid neutralization; yeast extract and nutrient salts. As nutrient salts magnesium, manganese, and iron sulfates, ammonium citrate, mono- and disubstituted potassium phosphate. Formed lactic acid is neutralized is carried out over 24 h from fermentation process starting by introducing of 40 % of rest chalk. Method of present invention makes in possible to increase of total process productivity by 236 % and lactic acid yield by 8 %.

EFFECT: method of increased productivity and yield.

2 cl, 4 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention relates, in particular, to preparing L-(+)-lactate by microbiological synthesis. The strain Bacillus coagulants SIM-7 is isolated from overheated grains and deposited at number DSM 14043. This strain is used in a method for preparing L-(+)-lactate by its culturing at temperature 53-65°C in medium containing partially fermenting sugars. Invention provides preparing L-(+)-lactate at higher temperatures wherein its the yield is above 95%.

EFFECT: valuable properties of strain, high yield of product.

5 cl, 2 ex

FIELD: biotechnology.

SUBSTANCE: new lactic acid Lactobaccillus delbrueckii strain B-8744 strain as lactic acid producer is isolated from specific Lactobaccillus delbrueckii strain by selection of spontaneous mutations. In process of lactic acid fermentation with new strain on sugar-containing nutrient medium with sugar concentration of 12 % total productivity is 2.92-2.96 g/(l.h), and lactic acid yield is 93.9-95.2 %.

EFFECT: new genetically stable lactic acid Lactobaccillus delbrueckii strain of high productivity.

3 ex

FIELD: biotechnology, microbiology, genetic engineering.

SUBSTANCE: invention relates to methods for preparing lactic acid. Lactic acid is prepared by culturing the recombinant strain of yeast of genus Schizosaccharomyces as a producer of lactic acid comprising at least one foreign lactate dehydrogenase gene. The recombinant strain of yeast Schizosaccharomyces pombe VKPM Y-3127 is prepared by using the strain Schizosaccharomyces pombe VKPM Y-285 as a recipient and transformation of the strain is carried out with plasmid DNA carrying IdhA gene from fungus Rhizopus oryzae. Invention provides carrying out synthesis of lactic acid at low pH values and show capacity for producing lactic acid at pH 4.0 up to the concentration of lactic acid in medium 80-100 g/l.

EFFECT: improved preparing method.

3 cl, 5 dwg, 2 tbl, 4 ex

FIELD: biotechnology, in particular immobilized biocatalysts.

SUBSTANCE: invention relates to biocatalysts based on microorganism cells, incorporated in gel carrier matrix for microbiological lactic acid production. As immobilized biocatalyst matrix polymer-based cryogel in used, wherein polymer is selected from group containing polyvinyl alcohol, polygalactomannan, amylolytically stable starch, gelatin. Ratio of starting components (mass %) is: biomass of microorganism cells with lactate dehydrogenase activity 0.001-4.0 (calculated as dry material); polymer 2-15; and balance: water/aqueous buffer. Immobilized catalyst is obtained by biomass incorporating into cryogel matrix followed by immobilized cell treatment by incubation at 28-45°C for 10-200 h in medium capable of increasing lactate dehydrogenase activity of immobilized biomass. Lactic acid is obtained by substrate solution treatment with said immobilized biocatalyst at pH 5.5-6.5 and 28-45°C for 15-24 h followed by target product isolation by known methods.

EFFECT: strength carrier with high porosity; biomass with increased activity; effective and safe method for lactic acid production.

3 cl, 7 ex

The invention relates to processes and equipment for automated processing of municipal solid waste (MSW) (from the dump or obtained directly from urban services), sewage sludge and tire waste for disposal and utilization of all usable materials for the industrial production of lactic acid

FIELD: biotechnologies.

SUBSTANCE: proposed ferments are as follows: hydrogenase and dehydrogenase extracted from Thermococcus onnurineus NA1 and having the sequences specified in the description, as well as their coding genes. A vector containing the above genes combined into FDH2-MFH2-MNH2 cluster is described. A host cell containing the above vector is described. Hydrogen production methods involving the following stages are proposed: preparation of medium in a cultivation vessel; supply of formiate to gaseous phase present in the cultivation vessel; cultivation of recombinant host cell or strain T. onnurineus in the cultivation vessel; extraction of hydrogen from the cultivation vessel.

EFFECT: invention allows more effective production of hydrogen by means of a microbiological method in the medium containing gaseous phase of formiate.

13 cl, 14 dwg, 7 tbl, 4 ex

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