Application of anabaena sp. pcc 7120 strain for silver nanoparticles production

FIELD: biotechnology.

SUBSTANCE: during biological recovery of silver with nanoparticles production, incubation is performed under constant illumination of the Anabaena sp. PCC 7120 in nitrogen-free medium with silver nitrate.

EFFECT: invention provides a solution of silver nanoparticles, wherein the nanoparticles exist in a free state.

 



 

Same patents:

FIELD: power engineering.

SUBSTANCE: objective of the invention is to convert an operable periodical biogenerator plant with manual charging-discharging for production of low-pressure biogas into an industrial continuous plant for production of biogas (biohydrogen) of high pressure (10-12 MSh) by installation of a bioreactor in the ground at the depth of around 2000 m, which will provide for optimal temperature conditions of reactions of anaerobic conversion of biomass, will develop conditions for self-flowing charging of a bioreactor with biomass, gaslift discharging of biogas and residual biopulp. For production of biohydrogen the following systems are provided: for biomass acidification up to pH=5.49: sowing of biomass with hydrogen-producing microorganisms; supply of biological catalyst into a zone of reaction of the bioreactor. Standard drilling equipment and materials may be used for arrangement of continuous underground generators of biogas (biohydrogen).

EFFECT: invention is ideally energy-saving and environmentally safe.

FIELD: chemistry.

SUBSTANCE: invention relates to field of biotechnology. Claimed is device for obtaining nanoparticles by reduction of metals from initial salts in presence of cultivated cells of microorganisms. Device includes control computer (1), connected with it electronic block of regulation and control (2) of all functional units and blocks of fermenter (3), pH-stabilising block (4) with pH sensor (5) and hoses for supply of titering solutions by pumps (6, 7), block (8) for regulation of redox-potential of culture mixture, provided with redox sensor (9), independently controlled pumps (10, 11) for introduction of initial solutions of metal salts, reducing agents and growth factors into fermenter (3), block (12) for regulation of dissolved oxygen level with sensor pO2 (13), pump (14) for supply of growth substrate, block (15) for measurement of optic culture density with application of optic fibre sensor (16), block (17) for measurement of spectral characteristics of culture mixture with application of optic fibre sensor (18), isolated with impermeable for cells membrane with pore size 100-250 nm, block (19) for thermoregulation of fermenter (3), equipped with temperature sensor (20), block (21) for regulation of culture mixture mixing, which brings into motion blade mixer (22), block (23) for regulation of culture mixture illumination in case of cultivating phototrophic microorganisms and control of spectral parameters of submersible diode lamp (24), block (25) for ultrafiltration of sampled culture mixture with sterilising membrane with pore size 100-250 nm with possibility of output of only nanoparticle suspension from fermenter, condenser of output moisture (26), preventing loss of culture mixture.

EFFECT: invention contributes to extension of arsenal of technological methods of obtaining nanoparticles of metals and makes it possible to achieve controllability of modes of nanoparticle formation.

2 dwg, 3 ex

FIELD: biotechnologies.

SUBSTANCE: proposed ferments are as follows: hydrogenase and dehydrogenase extracted from Thermococcus onnurineus NA1 and having the sequences specified in the description, as well as their coding genes. A vector containing the above genes combined into FDH2-MFH2-MNH2 cluster is described. A host cell containing the above vector is described. Hydrogen production methods involving the following stages are proposed: preparation of medium in a cultivation vessel; supply of formiate to gaseous phase present in the cultivation vessel; cultivation of recombinant host cell or strain T. onnurineus in the cultivation vessel; extraction of hydrogen from the cultivation vessel.

EFFECT: invention allows more effective production of hydrogen by means of a microbiological method in the medium containing gaseous phase of formiate.

13 cl, 14 dwg, 7 tbl, 4 ex

FIELD: chemistry.

SUBSTANCE: phytoplankton is cultivated in electromagnetic bioaccelerators. Oxygen and biomass, consisting of lipids, hydrocarbons and sugars are obtained. Biomass is oxidised. Thermodynamic energy is obtained. Carbon dioxide and NOx are returned to electromagnetic bioaccelerators.

EFFECT: invention makes it possible to obtain biofuel and reuse CO2 and NOx.

9 cl, 10 dwg

FIELD: medicine.

SUBSTANCE: method for preparing a composition containing colloidal nanosilver or nanogold involves incubation of probiotic bacteria specified in Lactobacillus fermentum species with an aqueous solution containing at least 4 mM of silver nitrate or auric chloride. The composition containing colloidal nanosilver is prepared by contact of said bacteria at 5-45C and the aqueous solution containing mixed silver nitrate, ammonium and/or ammonia salt, as well as alkali metal hydroxide. The composition containing colloidal nanogold is prepared by contact of said bacteria at 5-45C and the aqueous solution containing mixed auric chloride and alkali metal hydroxide.

EFFECT: prepared compositions are used as an antimicrobial agent or an algicidal agent.

17 cl, 4 dwg, 5 tbl, 16 ex

FIELD: medicine.

SUBSTANCE: there are presented enzymes: hydrogenase and dehydrogenase recovered from Thermococcus onnurineus NA1 and having the sequences presented in the description, as well as genes coding them. What is described is a vector containing said genes combined in the cluster CODH-MCH-MNH3. What is created is a host cell containing said vector. There are presented method for producing hydrogen involving the stages as follows: preparing a medium in a cultivation bottle, cultivating the recombinant host cell or the strain Thermococcus onnurineus NA1 in the presence of carbon monoxide, recoverying hydrogen from the cultivation bottle.

EFFECT: invention enables the most effective way to produce hydrogen by microbiological technique.

12 cl, 10 dwg, 5 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: there is offered a composition containing a combination of at least two phytases for hydrolysis of phytic acid (hexoctaphosphate myoinositole) to inorganic monophosphates, to phosphate myoinositole with smaller degree of phosphorylation and to free myoinositole. Said compositions are used in making a feed supplement or animal feedstuff. Also, there is offered a method of phytic acid hydrolysis with using the phytase combination. The compositions under the invention are catalysed by rapid and complete phytic acid hydrolysis in the wide range of pH values.

EFFECT: higher availability of phytic acid phosphorus and improved digestibility of animal feedstuff.

21 cl, 3 dwg, 4 tbl

The invention relates to the environment, and in particular to processes of preparing gases for inhalation, and can be used in enzyme-catalytic oxygen in the Autonomous life support devices used in medicine

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Strain of spore bacteria Bacillus megaterium 501 GR, possessing growth-stimulating effect, is deposited in ARRIAM RAAS under registration number RCAM00875 and can be used as polyfunctional means for plant growing, improving phosphorus feeding of plants, as well as promoting bioremediation of pesticide-polluted soils.

EFFECT: invention makes it possible to increase plant productivity, as well as to protect plants from phytopathogenic fungi and microorganisms, causing diseases of plant roots.

13 tbl

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Halobacterium salinarum RNCIM B-11850 - producer of bacteriorhodopsin is described. The strain of Halobacterium salinarum ST 3 is obtained by multistage selection of halophilic bacteria strain Halobacterium salinarum RNCIM B-10425 without preliminary treatment with chemicals and is deposited under the number RNCIM B-11850.

EFFECT: strain Halobacterium salinarum RNCIM B-11850 exceeds the prototype RNCIM B-10425 in productivity.

2 tbl, 2 ex

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Halobacterium salinarum D96N RNCIM B-11953 - producer of bacteriorhodopsin, significantly surpassing in productivity (biomass and bacteriorhodopsin) as the closest analogue, as well as others selected as controls strains under different conditions of culturing.

EFFECT: increased properties of the strain.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to the strain of Bifidobacterium longum NCIMB 41675, a composition, containing thereof, and a food product, containing the said strain or composition. The claimed strain possesses an ability to induce the production of cytokins and control IL-10:IL-12 ratio is suitable for application in immunomodulation, treatment of an autoimmune disease.

EFFECT: claimed strain, composition and food product possess probiotic properties.

25 cl, 16 dwg, 4 tbl, 8 ex

FIELD: electricity.

SUBSTANCE: metal-dielectric structure and method of its manufacturing are related to electronic industry and electronic engineering and may be used both in modern energy-saving systems and components being an integral part of modern processors, in particular for development of microsized and nanosized electromechanical systems. The metal-dielectric structure consists of dielectric and conducting layers made as an assembly of capillaries filled with metals to the required length, at that conducting layers are etched on selective basis at different butt ends and metalised. The conducting layers are represented by two different types of electroconductive materials etched on selective basis at different butt ends, at that the conductive layers may be made of semiconductor materials, conducting glass, carbon nanoparticles and nanotubes while the dielectric layers may be made of optical and electron-tube glass, polymer materials. In cross-section the dielectric and conducting layers may be made as concentric circles. The manufacturing method for the above metal-dielectric structure includes assembly, overstretching, stacking to the unit, at that upon multiple overstretching vacuum filling with conducting materials is performed, and butt ends are etched on selective basis with different chemical composites and then they are metalised.

EFFECT: invention allows increasing capacitance and breakdown voltage for capacitors.

5 cl, 7 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to pressure sensitive adhesives, suitable for use on a wide variety of substrates, including both high surface energy and low surface energy substrates. Pressure sensitive adhesives comprise an acrylate polymer and surface-modified nanoparticles. The surface-modified nanoparticles comprise a nanoparticle comprising a silica surface and surface modifying groups, covalently bonded to the silica surface of the nanoparticle. At least one surface modifying group is a polymeric silane surface modifying group. At least one surface modifying group is a non-polymeric silane surface modifying group.

EFFECT: disclosure also provides a method of preparing pressure sensitive adhesives, including exposing them to UVA and UVC radiation.

21 cl, 2 dwg, 12 tbl

Up!