Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology and can be used to create bioprotection of plants against phytopathogens and to stimulate their growth. Group of inventions comprises the strains of the fungus of Trichoderma longibrachiatum species (3 options); biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of these strains and the method for producing a biological product. Strains of the Trichoderma longibrachiatum fungus are deposited at the ARRIAM FSBSI under registration numbers: RCAM 03324, RCAM 03323, RCAM 03325 respectively. Biological product contains spores and fragments of mycelium of above strains from 2×109 to 4×109 CFU/cm3; has antagonistic activity against phytopathogenic fungi of the Fusarium and Cladosporium species. Method of producing a biological product involves a two-stage joint cultivation of strains in a quantitative ratio of 1:1:1 consistently on two nutrient media for 4–5 days. This group of inventions provides increase in seed germination on 4–15 % and the increase of linear sizes of plants on 0.5–24 %.

EFFECT: increased seed germination and the increase of linear sizes of plants.

5 cl, 2 tbl, 5 ex

 



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Rhodococcus sp. LER-12 having the ability to dispose quickly of crude oil and petroleum products (diesel fuel, motor oil, hydraulic oil, gas condensate) is deposited in the RNCM under the registration number Rhodococcus sp. RNCM Ac-2626D and can be used for purification of soils and water from contaminations with crude oil and petroleum products, in a wide temperature range from +4 to +30°C.

EFFECT: invention enables to improve the quality of purification of soil and water from crude oil and petroleum products.

3 tbl, 5 ex

FIELD: biotechnology.

SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.

EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.

4 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: nutrient medium comprises sodium chloride, potassium chloride, magnesium sulphate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, sodium bicarbonate, L-arginine, L-glutamine, L-tyrosine, L-tryptophan, calcium pantothenate, pyridoxal HCl, thiamine, meso-inositol, nicotinamide, riboflavin, folic acid, glucose, enzymatic hydrolyzate of muscle proteins, lactalbumin enzymatic hydrolyzate, sodium salt of benzylpenicillin with activity of 0.09×105-1.1×105, kanamycin sulphate with activity of 0.09×105-1.1×105, nystatin with activity of 6×104 to 6.5×104 IU/l, choline chloride, succinic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to improve the quality of the product obtained.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.

EFFECT: invention makes it possible to increase the output of citric acid.

2 tbl, 6 ex

FIELD: biotechnologies.

SUBSTANCE: method of production of nanodisperse additive for concrete is offered. Cyanobacteria of the sort Pseudanabaena sp. 0411 or Leptolyngbya laminosa 0412 are cultivated in a nutrient medium at the temperature 23-25°C. The nutrient medium is Z-8 medium with adding of sodium silicate solution neutralized by 2 M HCl. Ratio of solution of silicate of sodium and Z-8 5:1 medium. Cultivation is carried out in the bioreactor at continuous lighting and mixing during 10 days with the subsequent removal of nutrient medium residue. Then the culture is poured by 30% hydrogen peroxide solution and heated up to 70°C. The produced biosilicated nanotubes are washed out by distilled water with subsequent treatment in a mechanical activator in the water medium of anion surface-active substance of naphthalene formaldehyde type at the ultrasound frequency 35 kHz and concentration of solid phase 5% up to the particle size 85-250 nanometres.

EFFECT: increase of mobility of concrete mix, curing acceleration, increase of strength and density of concrete, decrease of water absorption.

3 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanovermiculite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanobentonite and distilled water.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 14 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nano sapropel and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of phosphate-mobilising and nitrogen-fixing microorganisms.

1 tbl, 15 ex

FIELD: biotechnologies.

SUBSTANCE: nutritive medium contains potassium dihydrophosphate, potassium hydrophosphate, magnesium sulphate heptahydrate, sodium chloride, calcium sulphate dihydrate, sodium molybdate, iron (II) sulphate, saccharose, nanozeolite and distilled water with a defined component ratio.

EFFECT: enhanced growth rate of nitrogen-fixing and phosphate-mobilising microorganisms.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns the strain Escherichia coli BL21(DE3)Gold/pETmin-CypA producing human recombinant cyclophilin A. The characterised strain is produced by cell transformation of the strain BL21(DE3)Gold by pETmin-CypA plasmid. The plasmid has a size of 5865 base pairs and contains XhoI-NdeI fragment of pET-22b(+) vector carrying ampR ampicillin resistance gene, a promoter and RNA-polymerase T7 phage terminator and a polylinker. A fragment produced by PCR with using the oligonucleotide primers 5'-TTATACATATGGTCAACCCGACCGTGTTCTTC and 5'-TTTCTCGAGTTATTCGAGTTGTCCACAGTCAGC of pCyPAwt/pGEX-2TK plasmid with the closed fragment of hrCpA (human recombinant cyclophilin A) gene having a size of 498 base pairs is cloned at XhoI-NdeI sites.

EFFECT: presented strain is high-producing; it requires no complicated clean-up system and can be used in cancer treatment.

2 dwg

FIELD: biotechnology.

SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.

EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.

4 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.

EFFECT: invention makes it possible to increase the output of citric acid.

2 tbl, 6 ex

FIELD: biotechnology.

SUBSTANCE: strain Aspergillus oryzae 12-84, having a high level of synthesis of the complex of proteases and peptidases, nucleases, chitinase, β-glucanase, mannanase and α-amylase, is deposited in State Scientific Institution of All-Russian Scientific Research Institute of Agricultural Microbiology of the Russian Agricultural Academy under the registration number Aspergillus oryzae RCAM01134. The strain may be used to produce complex enzyme preparations with their subsequent application for hydrolysis of raw materials in the preparation of biocorrectors of food and feed, amino acid additives and biologically active additives with functional properties.

EFFECT: invention enables to increase the yield of proteolytic, nuclease, chitinase, β-glucanase, mannanase and α-amylase activities.

2 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and specifically to a fermentation medium and a method of producing recombinant proteins using said medium. A fermentation medium for producing recombinant proteins selected from a group including GM-CSF, streptokinase and lipase, using microorganisms selected from a group including: E. Coli, Streptomyces sp. and Rhizomucor sp., is characterised by keeping concentration of urea or derivatives thereof in the range of 0.5 g/l to 2 g/l. The medium contains, per litre of water, basic salts in the following amounts: orthophosphoric acid (85%) 2.67-133.5 ml, calcium sulphate 0.093-4.65 g, potassium sulphate 1.82-91 g, magnesium sulphate-7H2O 1.49-74.5 g, potassium hydroxide 0.413-20.65 g, glycerine 4-200 g. The medium contains, per litre of water, trace elements in the following amounts: copper sulphate-5H2O 0.6-30 g, sodium iodide 0.008-0.4 g, manganese sulphate-H2O 0.3-15 g, sodium molybdate-H2O 0.02-1 g, boric acid 0.002-0.1 g, cobalt chloride 0.05-2.5 g, zinc chloride 2-100 g, iron (II) sulphate-7H2O 6.5-325 g, biotin 0.02-1 g, sulphuric acid 0.5-25 ml.

EFFECT: invention increases the output of end products.

6 cl, 27 dwg, 3 tbl, 16 ex

FIELD: biotechnology.

SUBSTANCE: strain of micromycete Aspergillus foetidus 379-K-5-1 is proposed, producing a complex of pectinases, β-glucanase, xylanase, cellulase, chitinase, mannanase and protease for the destruction of polysaccharides of a plant and microbial material. The strain is deposited in the Departmental collection of useful microorganisms for agricultural purposes of the Russian Academy of Agricultural Science (RCAM) of the State Scientific Institution of the Russian National Research Institute for Agricultural Microbiology under the registration number of RCAM 01136. The advantage of the new strain is to obtain the more active complex of enzymes catalyzing the hydrolysis of polysaccharides, as well as endopolygalacturonase and pectinesterase. The strain Aspergillus foetidus RCAM 01136 is produced using efficient methods of selection and mutagenesis using UV irradiation and nitrosoguanidine from the known strain.

EFFECT: invention enables to expand the range of the enzymes obtained, used for the destruction of polysaccharides.

2 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants against invasive and perennial weed vegetation of class Dicotyledones. The suspension is used on the basis of vegetative mycelium of phytopathogenic micromycetes with chemical herbicides in sublethal doses. The plants are treated by spraying an aqueous solution of the herbicide with the active substance of glyphosate, and suspension of mycelium of foma-like fungi. Treatment is carried out at all stages of plant development, first with herbicide at the flow rate by glyphosate active substance of 0.02-0.32 kg/ha, then by a suspension based on mycelium fragments of foma-like fungi in a concentration of from 25 to 100 g/l. The consumption rate of the working fluid is from 50 to 300 l/ha. The proposed method enables to reduce 1.5-10 times or more the chemical load on the ecosystem, 1.5-4 times the infectious load (concentration of phytopathogenic fungi mycelium).

EFFECT: method can be applied at all stages of plant development, without requiring additional protection of the cultivated crop.

10 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to method of obtaining 9-decen-2-one. Method includes cultivation of said mould, belonging to family Aspergillaceae or Mortierellaceae, addition of undecylenic acid as substrate in fermentation medium with consumption from 0.1 to 0.9 g/l/hour in presence of oil, substrate bioconversion in 9-decen-2-one, its extraction and purification. As oil, used is oil, selected from the group, containing traditional food oils or triglycerides, which contain short-chained fatty acids, or white oils.

EFFECT: invention makes it possible to obtain 9-decen-2-one with output 8-16,5 g/l and high degree of purification 99%.

13 cl, 1 dwg, 9 ex

FIELD: biotechnology.

SUBSTANCE: strain Trametes versicolor is proposed, used for production of antifungal agents against fungi of the genus Penicillium. The strain is deposited in the RCIM under the number F-1024.

EFFECT: strain has high chitinase and fungicidal activity.

4 dwg, 1 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used in agriculture. The Trichoderma harzianum Rifai strain is deposited in the Russian National Collection of Industrial Microorganisms under registration number VKPM F-180. The strain is capable of producing L-lysine-alpha-oxidase and can be used particularly as an inhibitor of cucurbit bacterial spot caused by Acidovorax citrulli bacteria.

EFFECT: invention reduces loss of cucurbit crop.

FIELD: biotechnologies.

SUBSTANCE: method for obtaining proteinase - protein C activator of blood plasma provides for solid-phase cultivation of fungus strain Aspergillus ochraceus BKM F-4104D on a substrate. The substrate contains the following components at a certain ratio: a solid substance or a carrier, glucose, amylum, hydrolysate of fish flour, peptone, NaCl, KH2PO4, MgSO4·7H2O, and water. As a solid substance or a carrier, vermiculite, polyurethane foam, silica gel or wheat middlings are used. Proteinase activity is up to 300 U/ml.

EFFECT: invention allows obtaining a target product with improved activity.

5 tbl, 15 ex

FIELD: chemistry.

SUBSTANCE: group of inventions relates to application of lipochitooligosaccharide of formula for stimulation of plant mycorhisation, where n=2 or 3, R1 represents lipid substituent, which represents fatty acid chain, containing from 16 to 18 carbon atoms, which can be saturated or mono- or di-unsaturated, and R2 represents H or SO3H. Also claimed is mixture of lipochitooligosaccharides for stimulation of arbuscular-mycorhisal plant symbiosis, for stimulation of plant seed germination or for stimulation of plant seed germination or for stimulation of plant root system development and containing in effective amount lipochitooligosaccharide of formula (I), where R2 represents H, and lipochitooligosaccharide of formula (I), where R2 represents SO3H.

EFFECT: group of inventions effectively stimulates plant seed germination, of arbuscular-mycorhisal plant symbiosis and plant root system development.

9 cl, 16 dwg, 9 ex

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