Method of sampling and preparation of the cattle wool samples for analysis of the elemental composition
SUBSTANCE: invention relates to agriculture and can be used for biomaterial sampling to obtain reliable data on the content of macro- and microelements in the wool of cattle. Method of sampling and preparation of samples of wool involves shearing of desired sample on weight of not less than 2 g from the top of the animal withers from the area of skin with the size of 5×5 cm. Obtained sample of wool is soaked in electrochemically disinfectant activated catholyte water with temperatures from +40 to +60 °C for 2–3 hours. Then it is washed under running water with great pressure until the visible contamination is disappeared. Then it is cleaned in ultrasonic baths with a working frequency of 35 kHz, using consistently detergent environments: 40 % ethyl alcohol, bidistillated water for 60 min in each environment. After it the sample is dried in a drying cabinet at +40 °C.
EFFECT: convenience of wool sampling from the less contaminated area of the skin is provided.
1 cl, 3 tbl
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of developing occupational hyperkeratosis. Substance of the method consists in recovering DNA from peripheral venous blood lymphocytes, genotyping TP53 gene rs1625895 polymorphism by polymerase chain reaction with restriction enzyme digest analysis. Finding G/A genotype and A allele ensured predicting a risk of developing occupational hyperkeratosis.
EFFECT: using the invention enables predicting developing occupational hyperkeratosis after the body has been exposed to occupational hazards.
2 tbl, 2 ex
SUBSTANCE: invention relates to the field of medicine, namely to a method of diagnosing gestational diabetes mellitus in pregnant women, which includes carrying out a blood test in the morning on an empty stomach with the determination of the venous plasma glucose content. The essence of the method consists in the fact that if the level of venous plasma on an empty stomach is lower than 5.1 mmole/l, detection of diabetes risk factors: obesity with an initial, pre-pregnancy, excess body weight of more or equal 30 kg/m2, or diabetes mellitus of 2 type in the nearest relatives, or impairment of carbohydrate exchange in past medical history, or glycosoria, in pregnancy terms before 24 weeks, determination of capillary blood glucose is performed by continuous monitoring for 3 days. If registered are: one or several indices of the level of glucose in venous plasma blood on an empty stomach higher or equal to 5.1 mmole/l, but lower than 7.0 mmole/l, one hour after meal, index of the level of glucose in venous plasma higher or equal to 10 mmole/l, 2 hours after meal, index of the level of glucose in venous plasma higher or equal to 8.5 mmole/l gestational diabetes mellitus is diagnosed.
EFFECT: application of the claimed method makes it possible to increase the efficiency of diagnostics of gestational diabetes mellitus in pregnant women.
3 dwg, 2 tbl, 1 ex
SUBSTANCE: diagnostic technique for the presence and stage of hepatic fibrosis accompanying chronic viral hepatitis C consists in determining the concentrations of gamma glutamine transferase, thrombocyte growth factor-β1, tissue inhibitor of metalloproteinases-1 and MMP-9/TIMP-2 complex in blood serum; the first stage involves calculating a probability of HCV-associated hepatic fibrosis (PPHF) by formula: PPHF=(ey/(1+ey))×100%, wherein y=-13.6+3.78·X1-0.1·X2 wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TIMP-1, ey is an exponential function. If PPHF>50%, the presence of HCV-associated fibrosis changes in the liver are predicted. At the second stage, a probability of the stage of the HCV-associated hepatic fibrosis (PSHF) is specified by formula: PSHF=(ey/(1+ey))×100%, wherein y=-12+0.134317·X1+0.527778·X2-0.05·X3, wherein X1 is a serum value of the prognostic factor MMP-9/TIMP-2, X2 is a serum value of the prognostic factor TGF-β1; X3 is a serum value of the prognostic factor of gamma glutamine transferase. If PSHF>50%, the presence of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis accompanying chronic viral hepatitis C (F3-4 stage); PSHF<50% shows HCV-associated hepatic fibrosis of the primary stage (F0-2 stage).
EFFECT: method is easy to use, accessible for practical medical facilities, non-invasive and safe for patients, efficient and takes no time; it is effective for screening of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis.
3 ex, 3 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology, namely, to obtaining inhibitors of adhesion and/or aggregation of platelets, and can be used in medicine. A polypeptide, used as a component of a pharmaceutical composition and in sets for screening of the inhibitors of platelet adhesion or aggregation, is obtained in a recombinant way with the application of a matrix of the salivary gland cDNA of Anopheles stephensi.
EFFECT: invention makes it possible to obtain the polypeptide, possessing inhibiting activity with respect to platelet aggregation and/or inhibiting activity with respect to platelet adhesion.
10 cl, 4 dwg, 5 ex
SUBSTANCE: invention relates to medicine, namely to dentistry, and can be used in diagnostics of dental caries by method of infrared spectroscopy. For this purpose sample of patient's saliva is preliminarily dries, dry residue is crushed and suspended in vaseline oil. IR-spectroscopy is carried out in spectrum area 1200-800 cm-1 and height of peaks of absorption bands with maximums 1070, 1017, 960, 860 cm-1 is determined. After that, values of four ratios of peak height with maximum at 1070 cm-1 to peak height with maximum 1017 cm-1, peak height with maximum at 1070 cm-1 to peak height with maximum 960 cm -1, peak height with maximum at 1070 cm-1 to peak height with maximum 860 cm -1, ratio of peak height with maximum at 1017 cm-1 to peak height with maximum 860 cm-1. Dental caries is diagnosed at the following values of ratios of peak heights 1070/1017, equal to 11.64±0.14, peak heights 1070/960, equal to 1.08±0.12, peak heights 1070/860, equal to 6.40±0.41, and peak heights 1017/860, equal to 4.15±0.44.
EFFECT: invention provides self-descriptiveness of diagnostics and increases its accuracy.
SUBSTANCE: method consists in production of cryostat sections of nerve, washing them in two portions of maleate buffer, incubation in incubation medium. Incubation is carried out for 30-60 minutes and incubation medium has the following composition: acetylcholine iodide 10 mg; 0.1 M Na-maleate buffer 2 ml; 0.1 M sodium citrate 4 ml; 0.03 M copper sulphate 4 ml; 0.005M potassium ferricianide 4 ml; medium pH constitutes from 7.4 to 7.6 and temperature is from 50 to 55°C.
EFFECT: method by invention makes it possible to evaluate viability of peripheral nerves in short term, which is necessary during reconstructive-restoring and highly technological neurosurgical operations.
SUBSTANCE: functional activity of neutrophilic granulocytes is studied by means of hemiluminiscent analysis, calculated is an index of formation of active forms of oxygen (IFAFO), representing the ratio of the area under the curve of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by a live bacterial suspension of staphylococcus aureus, to the time of achieving the maximum of luminol-dependent hemiluminiscence of the neutrophilic granulocytes, induced by the live bacterial suspension of staphylococcus aureus. If IFAFO value is higher than 100 o.u./s, sensitisation to staphylococcus aureus in patients with allergic rhinitis is diagnosed.
EFFECT: application of the claimed method makes it possible to diagnose staphylococcal allergy in case of allergic rhinitis before beginning therapy and contributes to the optimal selection of the patients' treatment.
1 tbl, 2 ex
SUBSTANCE: invention represents a method for determining a probability of preserving the myocardium following infarction by creating an admission examination-based data array of 7 peripheral blood parameters, 11 biochemical analysis parameters and 6 parameters of standard 12-lead electrocardiogram in 200 patients with the Q-myocardial infarction and 200 patients without the myocardial infarction. The parameters are stratified with respect to 7 intervals, wherein values related to the probability of preserving the myocardium following infarction are derived by calculating a ratio of the patients without myocardial infarction to all the patients with acute coronary syndrome. The probability is evaluated in a specific patient by analysing the above parameters, searching the respective intervals and values related to the probability of preserving the myocardium in the data array. Summing up the derived values enables calculating an integrated index, which is normalised, and a dimension from 0 to 100% is reduced.
EFFECT: invention enables increasing the prediction accuracy of preserving the myocardium in the patients with acute coronary syndrome.
1 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine, namely to immunology, and can be used in laboratory diagnostics, as a test system and a method for measuring interferon alpha (IFN-α) antiviral activity in human blood serum. The test system for measuring IFN-α activity in the human blood serum comprises diploid cells, a virus-containing fluid and standard human interferon-α (IFN-α). As the diploid cells, the test system comprises cells of the characterised line of the diploid cells - human M-20 fibroblasts at 20-33 passages cultured in the medium with added 10% fibrinolytically active plasma (FAP). As the virus, the system contains the A vesicular stomatitis virus (VSV) adapted to M-20 cells, the Indiana strain; the test system additionally contains a viral dye based on two fluorochromes - acriflavine and rhodamine C. The group of inventions also involves a method for measuring IFN-α activity in the human blood serum with the use of the developed system.
EFFECT: using the given inventions enables the quantitative good-reproducible measurement of IFN-α activity in the examined blood serum sampled by the luminescent microscopy of the vital preparations coloured in a fluorochrome-based vital dye.
3 cl, 4 dwg, 1 tbl, 4 ex
SUBSTANCE: invention describes a diagnostic technique for infected pancreonecrosis with establishing the indications for a surgical intervention by examining a patient, wherein acetic, propionic, butyric and isovaleric acids are measured in the blood by gas chromatography, and if the acetic acid concentration is more than 0.11 mmole/l enables stating infected pancreonecrosis, and if the concentration of any of three acids: propionic more than 0.095 mmole/l, butyric more than 0.0035 mmole/l, isovaleric acid more than 0.0003 mmole/l enables stating infected pancreonecrosis with active development of an anaerobic infection requiring one of versions of the surgical intervention.
EFFECT: invention enables providing the objective and accurate diagnosing of pancreonecrosis transformation into the stage of infection by using the qualitative parameters with no undesired side effects.
SUBSTANCE: saliva of affected child is analysed by method of infrared spectroscopy, value of ratio of peak height with maximum at 1070 cm-1 to peak height with maximum 1025 cm-1 is calculated. If value of ratio is in range from 1.1 to 1.9 nonspecific ulcerative colitis is diagnosed, and if value of said ratio is from 2.0 to 4.6 Crohn's disease is diagnosed.
EFFECT: invention ensures less invasive and simpler differential diagnostic of NUC and Crohn's disease in children, ensures simplification and acceleration of diagnosis determination.
SUBSTANCE: invention relates to medicine, namely to a method of diagnosing bipolar affective disorder. The essence of the method consists in the fact that reliable differences in the spectrum of protein distribution in blood serum without proteins albumin, immunoglobulin G, immunoglobulin A, antitrypsin, transferin and haploglobin in patients with endogenic psychosis. If protein spots are detected on electrophoretic gel in areas with the molecular weight of 200, 84, 75, 49, 40 kDa in the patient with endogenic psychosis, bipolar affective disorder is diagnosed.
EFFECT: application of the claimed method makes it possible to increase accuracy in diagnostics being simple in realisation.
SUBSTANCE: method involves determining female serum cytotoxicity to male lymphocytes, including a combined culture with reference male and analysed female serum in a 96-well tray in the presence of the nutrient medium RPMI 1640 in a CO2 incubator. One day later, lymphocytes are counted in the well in a Goryaev's chamber with the male (reference) and female (analysed) serum. That is followed by determining a cytotoxic index (CI), which represents a quotient of the analysed cell count and the reference cell count. The normal cytotoxic index makes approximately 0.7 and less.
EFFECT: invention enables studying the responses of female humoral immune factors to male antigens and evaluating a risk of miscarriage, early spontaneous abortions and missed miscarriages.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a high-sensitivity method for measuring the amount of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts. The high-sensitivity method for measuring the amount of glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts is characterised by the fact that a mixture of individual's blood plasma with methanol or ammonia water in the specific concentration is introduced into a solid phase having the reverse-phase distribution function and the anion exchange function; the solid phase is then washed with a cleaning fluid that is a single-component fluid or a mixed fluid of at least two components specified in a group containing water, alkali, alcohol and acetonitrile. That is followed by elution from the solid phase in acid alcohol specified in formic acid - methanol or formic acid - ethanol; that is followed by the stage of measuring glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts by liquid chromatography - mass spectrometry or liquid chromatography - mass spectrometry/mass spectrometry.
EFFECT: high-sensitivity method enables detecting and measuring the amounts of individual's blood plasma glycyrrhizin, glycyrrhetinic acid and their pharmaceutically acceptable salts.
4 dwg, 17 tbl, 7 ex
FIELD: veterinary medicine.
SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.
EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.
SUBSTANCE: patient's peripheral venous blood is recovered to analyse genetic polymorphisms of coagulation factors VII 10976G/A FVII. A birth weight of a newborn of a woman delivering not for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6123.431-25.579x1+0.267x2+205.739x3, wherein y is an anticipated newborn's weight, x1 is a female's height in centimetres; x2 is an infant's weight at the previous delivery in grams, x3 is a genetic version of 10976G/A FVII locus with x3=1 for 10976 GG FVII genetic type, x3=2 for 10976 GA and 10976 AA FVII genetic types. A birth weight of a newborn of a woman delivering for the first time in the stage of 37 and more weeks of pregnancy is determined by equation: y=6278.037-21.739x1+232.170x2, wherein x1 is a female's height in centimetres; x2 is a genetic version of 10976G/A FVII locus with x2=1 for 10976 GG FVII locus, x2=2 for 10976 GA and 10976 AA FVII genetic types.
EFFECT: invention enables improving the prevention and treatment of the pregnancy complications.
2 tbl, 4 ex
SUBSTANCE: pre-operative fasting venous blood 1 ml is sampled at room temperature 20-24°C into an anticoagulant-free vacuum system (test tube). The test tubes are delivered in a sealed container at temperature 2-8°C for 2 hours to a laboratory for immunoenzyme assay and analysed to determine anti-thyroid stimulating hormone receptor antibodies. If the antibody level is 1.5 units/l or more, a thyroidectomy is performed, whereas the antibody level of less than 1.5 units/l requires performing a subtotal thyroid resection according to standard techniques.
EFFECT: invention enables reducing a probability of recurrent thyrotoxicosis in the patients after subtotal thyroid resection.
SUBSTANCE: invention refers to medicine, namely to a method for the prediction of a risk of early microvascular complications in the children suffering from type 1 diabetes mellitus. The substance of the method consists in defining a duration of the diseases in years, the patient's age in years, a desquamated endothelial cell count, high-density lipoprotein cholesterol, total cholesterol, triglycerides, atherogenic index, glycohaemoglobin, average daily glycaemic level; making a linear regression analysis and calculating a risk ratio (R) of early microvascular complications in the children suffering from type 1 diabetes mellitus by formula. If the risk ratio is ≥1, the high risk of early microvascular complications during one year is predicted; the ratio < 1 shows the low risk of microvascular complications during one year.
EFFECT: using the declared method enables increasing the prediction accuracy of microvascular complications in the children with type 1 diabetes mellitus.
2 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine and can be used for patient's status monitoring. A method for setting a time-varying physiological parameter warning signal involves patient's controlled parameter monitoring, comparing the controlled parameter to an initial cut-off criterion, varying the cut-off criterion temporarily by a cut-off criterion of deterioration after the therapy, and then after a certain period of time, by the cut-off criterion after the administration. The time allowed involves comparing the controlled parameter to the cut-off criterion of deterioration, and after the time allowed - to the cut-off criterion after the administration. The warning signal is initiated in response to the controlled parameter of one or more initial cut-off criteria, the cut-off criterion of deterioration and the cut-off criterion after the administration. The group of inventions also refers to a machine-readable carrier with software for implementing the method and to a system for user warning on the controlled parameter variation.
EFFECT: group of inventions provides higher accuracy and rate of assessing the patient's status according to the conducted therapy.
15 cl, 6 dwg
SUBSTANCE: invention can be used for the purpose of the early prediction of cystic periventricular leukomalacia (PVL) in the newborns with very low (VLBW) or extremely low body weight (ELBW). Substance of the method: the newborns with VLBW and ELBW on the 3rd-7th day of life are examined to assess the perinatal medical history, namely the presence of chorioamnionitis and amniotic fluid nature, 5th minute Apgar score, the absence of prolonged artificial pulmonary ventilation, a severity of respiratory distress syndrome, the presence of pneumonia, sepsis, convulsive disorder, anaemia, laboratory signs of the systemic inflammatory reaction, average values of carbon dioxide, anionic bicarbonate and base deficiency in capillary blood, interleukine-6 and receptor interleukine-1 antagonist in venous blood serum. Each sign is assigned with a prognostic coefficient (PC). That is followed by determining total PC, and it is expected cystic PVL that is decided for if total PC is at least (+)9.5, whereas no cystic PVL is expected if total PC is (-)9.5 or less.
EFFECT: invention provides the early (for the first 7 days of life) prediction of cystic periventricular leukomalacia in the most susceptible category of patients that are children with very low and extremely low birth weight.
6 tbl, 5 ex
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.