Combined application of proteins vip3ab and cry1fa to generate insect resistance

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, namely to transgenic plant, which has resistance to antler moth (FAW; Spodoptera frugiperda), containing DNA, which codes Vip3Ab, DNA, which codes Cry1Fa, and DNA, which codes third protein selected from group, consisting of CrylC, CrylD and CrylE, its seed, as well as to method of preventing generation of resistance to proteins Vip3Ab and Cry1Fa in antler moth using it. Also set of plants in field, containing plurality of said transgenic plants and plants, which do not contain proteins of Bacillus thuringiensis (non-Bt plants), and mixture of seeds comprising seeds of non-Bt plants and multitude of said seeds are also disclosed. Invention also relates to composition for controlling lepidoptera pests, containing cells expressing Cry1Fa, Vip3Ab and third protein selected from group consisting of Cry1C, Cry1D and Cry1E, as well as to method of fighting antler moth using it.

EFFECT: invention provides effective fighting against antler moth.

34 cl, 3 dwg, 2 tbl, 8 ex

 



 

Same patents:

FIELD: food industry.

SUBSTANCE: invention refers to the field of biotechnology and food industry. Presented is a barley plant that yields grain and is homozygotic in at least two loci for the genetic variations having been bred, representing: a) allele wherein most of or all the genes coding B-hordein in Locus Hor2 are removed, and b) mutant allele in the barley Locus Lys3 so that the grain contains neither B-, nor C- hordeins, the said genetic variations present in Lines Riso 56 and Riso 1508 barley accordingly; absence of B-hordeins is to be revealed by absence of amplified DNA using primers: 5'B1hor: 5'-CAACAATGAAGACCTTCCTC-3', 3'B1hor: 5'-TCGCAGGATCCTGTACAACG-3', while absence of C-hordeins is to be revealed by absence of the 70 kDa strip during study of the grain alcohol-soluble extract by means of SDS-PAGE. Additionally presented are: barley grain cropped from the said plant; B- and C-hordein-free products produced from the said grain such as flour, malt and beer. Additionally described are methods for production of food products barley (flour, whole-grain flour, starch, malt) and beverages using grain cropped from the barley plant having the above characteristics. Proposed is a method for identification of barley grain suitable for production of a malt-based food product and/or beverage suitable for consumption by a person suffering from gluten-sensitive enteropathy which method includes: a) production of one or more materials: i) sample of a plant capable to yield the said grain, ii) grain, iii) malt produced from the grain, and/or iv) extract of the said grain; b) analysis of Stage a) material for presence of at least one hordein and/or at least one hordein-coding gene with selection of grain having the gene pattern of the above plant.

EFFECT: invention allows to manufacture B- and C-hordein-free malt-based food products or beverages.

27 cl, 14 dwg, 10 tbl, 10 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a method of producing sunflower seeds which contain endogenous oil, which contains at least 12% of stearic acid based on the total fatty acid content, in which oleic acid content is higher than the linoleic acid content, and in which the distribution coefficient of saturated fatty acids α between positions sn-1 and sn-3 is at least 0.28. Disclosed is oil obtained from sunflower seeds with at least 12% stearic acid based on the total fatty acid content, higher oleic acid content than linoleic acid content, and the distribution coefficient of saturated fatty acids α between positions sn-1 and sn-3 is at least 0.28, as well as a product containing said oil. Disclosed is a method of producing sunflower plant having seeds which contain endogenous oil containing at least 12% stearic acid based on the total fatty acid content, and in which the distribution coefficient of saturated fatty acids α between positions sn-1 and sn-3 is at least 0.28.

EFFECT: invention enables to efficiently produce oil containing stearic acid, with higher content of oleic acid than that of linoleic acid, and a distribution coefficient of saturated fatty acids α between positions sn-1 and sn-3.

12 cl, 3 dwg, 8 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: present inventions relate to protein engineering, plant molecular biology and pest control, as well as a hybrid insecticide protein and use thereof. Described is a hybrid insecticide protein which includes from the N-end to the C-end an N-end portion of Cry3A protein which is fused with the C-end portion of Cry1Ab protein, wherein the position of the crossover of the Cry3A protein and the Cry1Ab protein is located in a conservative block 2, in a conservative block 3 or in a conservative block 4 and has anti-western corn rootworm activity. Also disclosed are nucleic acid molecules which code the novel proteins, methods of producing proteins, methods for use thereof, as well as transgenic plants and seeds thereof which contain such proteins.

EFFECT: inventions enable to obtain cheap means of controlling Diabrotica worms.

39 cl, 8 dwg, 9 tbl, 46 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of biochemistry, in particular, to oil of sunflower seeds of quality varieties, having a fatty acid profile comprising 3% or less of total content combined together palmitic acid (16:0) and stearic acid (18:0).

EFFECT: invention enables to reduce the level of plasmatic cholesterol which causes coronary heart disease.

3 cl, 3 dwg, 6 tbl, 5 ex

FIELD: food industry.

SUBSTANCE: invention relates to food and agricultural branches, in particular, to sunflower oil extracted from seeds. Sunflower seeds oil contains palmic and stearic acids in an amount of 15% - 45% of all fatty acids present in oil, oleic acid in an amount of 45% - 75% of all fatty acids present in oil, gamma- and delta- tocopherols sum exceeds 85% of all tocopherols present in oil. The invention relates to a mixture of oils containing the said sunflower seeds oil. The invention relates to press cake produced as oil extraction processes residue. The invention relates to the said oil application for human and animal alimentation and to a technology for production of biolubricant substances and biofuel.

EFFECT: sunflower seeds oil has a high thermal stability.

30 cl, 3 tbl

FIELD: medicine.

SUBSTANCE: what is described is a method for producing Botrytis-resistant tomato involving a) producing Botrytis-resistant donor tomato plant preferentially of S. Habrochaites species or S. habrochaites LYC 4/78 line, b) transfer of nucleic acid from said donor plant into one or more Botrytis-sensitive recipient tomato plants, c) selection among the recipient tomato plant of the one containing at least one quantitative trait locus (QTL) in a genome for Botrytis-resistance from said Botrytis-resistant donor plant wherein said selection involves detection of one genetic marker related to said at least one QTL for Botrytis-resistance on 4, 6, 9, 11 and/or 12 chromosome of said recipient tomato plant. What is presented is a method for producing the inbred tomato plant involving breeding of Botrytis-resistant tomato plant with the same plant or the other tomato plant. Besides, there are described DNA produced from the tomato plant of S. lycopersicum species, containing QTL specified in a group consisting of QTL on chromosomes 4, 6, 9, 11 and 12 Solarium habrochaites LYC 4/78; Botrytis-resistant tomato plant and Botrytis-resistant portion of the tomato plant. What is also presented is a method for detection of QTL for Botrytis resistance involving detection of at least one genetic marker coupled with QTL for Botrytis resistance formed of S. habrochaites LYC 4/78 on 4, 6, 9, 11 and/or 12 chromosome. What is presented is the use of the genetic marker specified in a group consisting of the genetic markers presented in Tables 1-5 in the description for detection of QTL for Botrytis resistance.

EFFECT: invention provides producing Botrytis-resistant tomato plant.

20 cl, 31 tbl, 8 dwg, 4 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of biochemistry. Seeds of transgenic corn is described containing more than 4000 parts per million (ppm) of free lysine, where seeds of corn contain native DNA and one or more exogenous cassettes for expression in plants containing a DNA molecule encoding a dihydrodipicolinatsynthase resistant to inhibition by free L-lysine by the feedback principle, the DNA molecule, transcribed to produce RNA molecule, which suppresses lysinketoglutarate reductase/ sugar pindehydrogenase, and a DNA molecule encoding aspartatkinase resistant to the action of lysine-mediated feedback, where the DNA molecules are functionally linked to one or more promoter molecules in such a way that transcription of one or more RNA molecules occurs mainly in the endosperm of corn seed, which, optionally, the corn seed contains an exogenous gene, which confers resistance to herbicides. A method of obtaining corn flour is proposed, including obtaining of the said corn seed and seed processing to obtain flour. Flour is described obtained by this method. Use of the said seed is proposed to produce fine flour or coarse flour, animal feed, obtaining protein concentrate or isolate, and use of this seed by a human or an animal.

EFFECT: invention enables to obtain food products rich in lysine that do not require further addition of lysine.

11 cl, 2 tbl, 8 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: invention can be applied for obtaining promoter, which can be used to perform impact on physiological changes of metabolism in storing organs of plants after harvesting.

EFFECT: promoter has organ-specific activity in plants, demonstrates higher activity in storing organs of plants during storage, than in other plant organs, and its activity after harvesting storing organs changes and is higher, than before harvesting storing organs.

2 cl, 7 dwg

FIELD: agriculture.

SUBSTANCE: varieties of sunflower O11601A, O12653R, O11601B and E83329, having high content of oleic acid and resistance to imidazolinon, producing seeds, deposited in ATSS under deposit numbers PTA-9470, PTA-9472, PTA-9471 and PTA-9473, accordingly, are crossed with other inbred plant.

EFFECT: collected hybrid seeds of sunflower have content of oleic acid of more than 80% in weight.

3 cl, 16 tbl, 4 ex

FIELD: agriculture.

SUBSTANCE: specified plant is a plant of Cucumis sativus type and includes at least one area at one chromosome, which gives resistance to closterovirus, and at least one area, which gives resistance to powdery mildew. The area of the chromosome, which gives resistance to closterovirus, is linked to at least one marker selected from the group, made of markers E16/M50-244, E16/M50-188, and E11/M48-251. The area of the chromosome, which gives resistance to powdery mildew, is linked to at least one marker selected from the group made of the following components: a marker of single-nucleotide polymorphism 39TG in SEQ ID NO:1, a marker of single-nucleotide polymorphism 29GA in SEQ ID NO:2, a marker of single-nucleotide polymorphism 193CT in SEQ ID NO:3, mutation of an insert 5'-AATTT-3' in position 221 in SEQ ID NO:4, and markers E16/M50-F-194, E11/M48-F-251, E23/M38-M001, E23/M40-M003, E24/M46-M002, E24/M46-M003, E12/M91-M003, E26/M43-M003, E14/M59-F-134 and E14/M59-F-200.

EFFECT: production of the plant resistant to closterovirus and to powdery mildew of cucumbers.

25 cl, 8 dwg, 4 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to methods of producing a plant with higher drought and salt tolerance compared to the wild-type plant by reducing expression/function of the protein transcription factor in the plant. The invention also relates to a plant with high drought and salt tolerance obtained using said method.

EFFECT: invention enables to efficiently obtain a plant with high drought and salt tolerance compared to the wild-type plant.

6 cl, 6 dwg, 1 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the field of chemistry, in particular to the transgenic plant Brassica juncea, in seeds of which the endogenic content of fatty acids includes from approximately 70 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid, where the plant contains fad.2 and fad.3 allele, coding a mutant protein of delta-12-desaturases of fatty acids, which has an amino acid sequence, selected from the group, consisting of SEQ ID NO:5-7, as well as to the seed of the said plant and to oil of the said seed, the content of fatty acids of which includes from approximately 70.0 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid, which in the rest has properties of the Brassica juncea seeds.

EFFECT: invention makes it possible to effectively obtain oil of the Brassica juncea seeds, in which the content of fatty acids includes from approximately 70 wt % to 78 wt % of oleic acid and from approximately 2 wt % to 3 wt % of linolenic acid.

7 cl, 4 dwg, 6 ex

FIELD: chemistry.

SUBSTANCE: invention refers to biochemistry, particularly to a method for weed control near sunflower plants. The method involves applying an effective amount of any of the herbicides specified in a group consisting of imidazolinone herbicide, sulphonylurea herbicide, triazolopyrimidine herbicide, pyrimidinyloxybenzoate herbicide and their mixture, on weeds and sunflower plants. The above sunflower plant contains in its genome at least one copy of at least acetohydroxyacid synthase (AHAS) gene coding a herbicide-resistance protein having an amino acid sequence presented by SEQ ID NO:2.

EFFECT: invention enables controlling weeds near sunflower plants effectively.

9 cl, 13 dwg, 14 tbl

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and virology. What is presented is a method for producing viral-like influenza virus particles (IVP) in a plant or a part thereof. The method involves the expression of a new influenza virus protein HA in plants and purification thereof. The invention also aims at IVPs containing the influenza virus protein HA and herbal lipids. The invention also refers to nucleic acids coding an improved influenza virus HA, as well as to vectors. The IVPs can be used in developing the influenza vaccines or for the treatment of existing vaccines.

EFFECT: presented group of inventions can be used in medicine.

18 cl, 44 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to biochemistry, particularly to a gene expression construct containing: (a) an expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, b) a polylinker for facilitating a gene insertion into a gene expression system. What is disclosed is a gene expression vector containing the above construct and heterologous gene. What is also described is the gene expression vector containing: (a) the expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, (b) the heterologous gene, which codes a protein of interest, as well as a method for expressing the protein of interest with using it. A method for increasing translation enhancement activity of the heterologous protein of interest from the heterologous gene coding the above protein, a method for translation enhancement of the heterologous protein of interest from the heterologous gene or a heterologous open reading frame coding the above protein are disclosed. There are also described a host organism transformed by the above vector, as well as a method for producing the protein of interest, involving collection of the host organism tissue.

EFFECT: invention enables enhancing translation of the heterologous protein of interest from the heterologous gene.

26 cl, 15 dwg, 4 tbl, 6 ex

FIELD: biotechnology.

SUBSTANCE: expression cassette, the recombinant vector, the seed and the plant are disclosed, comprising the said isolated nucleic acid molecule, as well as the host cell and the plant comprising the said expression cassette. The invention also relates to the use of the said fusion protein in the insecticidal composition and the method of control of European corn worm, and also the method for its preparation. Also the method of production of a transgenic plant is disclosed, which is resistant to European corn worm, with use of the said fusion protein.

EFFECT: invention enables to obtain plants resistant to European corn worm.

39 cl, 8 dwg, 9 tbl, 45 ex

FIELD: agriculture.

SUBSTANCE: invention relates to a method of weed destruction, providing the step of applying the herbicide of PPO-inhibitory type on the cultivated part of the plant, where the said plant is resistant to the said herbicide and expresses the protein with the ability to convert the said herbicide into the herbicide with lower herbicidal activity. The method of evaluation of resistance of the plant cell or the plant to herbicide of PPO-inhibitory type is disclosed, comprising bringing into contact of the herbicide of PPO-inhibitory type with the plant cell or the plant into which DNA is introduced and expressed, which encodes the protein capable to convert the said herbicide into the herbicide with lower herbicidal activity, and also the method of intake of plant cell or plant resistant to the herbicide of PPO-inhibitory type, providing the step of intake of the plant cell or plant based on the aforesaid method for evaluating the stability. Also the plant cell, the plant, the cell culture of plant are disclosed, resistant to herbicide of PPO-inhibitory type, as well as the method of treating the herbicide of PPO-inhibitory type and use of the protein or DNA encoding the said protein for treatment of herbicide of PPO-inhibitory type, where the protein is capable to convert the aforesaid herbicide into the herbicide with lower herbicidal activity.

EFFECT: invention enables to destroy selectively only the weeds on the cultivated part of the plant.

10 cl, 66 dwg, 35 tbl, 75 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to a plant, having high resistance to an AHAS-inhibiting herbicide, which includes at least one Shiloh-8 IMI nucleic acid, parts thereof, a plant cell and seeds. Described is a nucleic acid which encodes a polypeptide which increases herbicide resistance of a plant. Disclosed are an expression cassette and a plant transformation vector which include said nucleic acid. Described are methods of controlling weeds growing near a plant having high resistance to an AHAS-inhibiting herbicide. Disclosed is a method of producing a plant having high resistance to an AHAS-inhibiting herbicide, as well as a method of increasing AHAS activity in a plant. Described is a method of selecting a cell transformed by a vector containing IMI nucleic acid. Also disclosed is a method of increasing resistance to an AHAS-inhibiting herbicide and a weed control method which includes treatment with an AHAS-inhibiting herbicide.

EFFECT: invention enables to obtain a plant which is resistant to an AHAS-inhibiting herbicide, which provides effective control of weeds growing near said plant.

57 cl, 3 dwg, 5 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: leaf explants isolated from the thirty-day aseptic plants of initial varieties grown in 1 l containers, are placed in Petri dishes with the liquid medium of a certain composition and preliminary incubated, co-cultivated and cultivated on nutrient media of a certain composition. When appearance of regenerants of the size at least 10 mm they are cut and transferred to the nutrient media of a certain composition. The first stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, by culturing the cut regenerants on MS medium with 50 ml/L kanamycin sulfate for 30-45 days at 22-25°C and illumination of 6000-10000 lux at 16-hour photoperiod followed by selection of rooted regenerants of potatoes with green leaves and stems. The second stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, including checking of rooted plants by PCR method for the presence of target DNA and reproduction of regenerants with confirmed PCR target DNA insert by micrografting.

EFFECT: use of claimed method enables to obtain potato forms resistant to Phytophthora and Alternaria spot causative agents.

5 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biochemistry, in particular to a method of increasing biomass or improvement of cell proliferation, leaf development and/or reproductive development of a plant, which includes increasing expression in the plant of nucleic acid, coding the ubiquitin-specific protease, as well as to the plant, a part of the plant and a seed of the plant, biomass of which is increased, or cell proliferation, leaf development and/or reproductive development of which are increased by the said method. A vector for application in the method of obtaining plants with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the plant and the part of the plant, transformed with the said vector, are described. Application of nucleic acid, coding the ubiquitin-specific protease is disclosed. Also disclosed is a method of obtaining a transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development, as well as the transgenic plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development in comparison with the control plants, obtained as a result of increased expression of nucleic acid, coding the ubiquitin-specific protease.

EFFECT: invention makes it possible to efficiently obtain the plant with increased biomass or improved cell proliferation, leaf development and/or reproductive development.

17 cl, 11 dwg, 3 tbl, 28 ex

FIELD: biotechnology, molecular biology, biochemistry.

SUBSTANCE: invention relates to regulatory sequences. Method involves isolation of DNA molecule with nucleotide sequence SEQIDNO:2 or SEQIDNO:3 that is necessary for expression of the required encoding sequence. Then vector comprising any of indicated sequences and the required sequence is constructed followed by transformation a plant with the prepared vector. Invention provides preparing transgenic plants with regulating expression of the required gene.

EFFECT: improved preparing method.

19 cl, 1 tbl, 6 ex

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